• Lee Byeong-chun and Kang Seung-keun have been charged with faking, together with Wang Woo-suk, the results of embryonic stem cell research and of embezzling public funds for the studies. (asianews.it)
  • The use of embryonic stem cells, which can be produced through SCNT, in some stem cell research has attracted controversy. (wikipedia.org)
  • SCNT is a method of cloning mammalian cells that can be used to create personalized embryonic stem cells from an adult animal or human. (news-medical.net)
  • Embryonic stem cells are immortal, and have the potential to develop into any type of adult cell, even after months growing in culture dishes. (news-medical.net)
  • By transferring adult cell DNA into an embryonic stem cell, it is possible to create a line of immortal embryonic cells that are able to develop into any type of adult cell, genetically identical to the donor. (news-medical.net)
  • The cloning method is based on the fact that cytoplasmic factors in mature, metaphase II oocytes are able to reset the identity of a transplanted adult cell nucleus to an embryonic state. (news-medical.net)
  • In humans, a major roadblock in achieving successful SCNT leading to embryonic stem cells has been the fact that human SCNT embryos fail to progress beyond the eight-cell stage. (news-medical.net)
  • They derived several human embryonic stem cell lines from these cloned embryos whose DNA was an exact match to the adult cell that donated the DNA. (news-medical.net)
  • This was the first successful reprogramming of human somatic cells into embryonic stem cells using a cloning technique, SCNT. (news-medical.net)
  • Dermal fibroblasts were taken from a 35-year old male and a 75-year-old male and used to create embryonic stem cells. (news-medical.net)
  • The adult cell nuclei were transferred into metaphase-II stage human oocytes, producing a karyotypically normal diploid embryonic stem cell line from each of the adult male donor cells. (news-medical.net)
  • Although attempts have not yet been made to create a therapeutic transplant from embryonic stem cells, the methods have been developed to allow the creation of functional, mature cells using human cell cloning technology. (news-medical.net)
  • Instead, embryonic stem cells are isolated from the cloned blastocyst. (eurostemcell.org)
  • To date, no human embryonic stem cell lines have been derived using therapeutic cloning, so both these possibilities remain very much in the future. (eurostemcell.org)
  • If research involving human embryonic stem cells (ESCs) is to achieve its potential for creating breakthrough medical therapies, additional new cell lines should be created, and therapeutic cloning--or somatic cell nuclear transfer (SCNT)--should be employed, according to an expert panel of the National Academy of Sciences. (the-scientist.com)
  • Given the promise of stem cell research for treating and perhaps curing a variety of debilitating diseases, our committee felt strongly that research not be limited, but include work on both human adult and embryonic stem cells," stated committee chair Bert Vogelstein , professor of oncology and pathology at the Johns Hopkins University School of Medicine, in. (the-scientist.com)
  • But his fall from grace came swiftly at the end of 2005 and has been gathering momentum since the beginning of 2006 when a scientific verification panel found he had created no embryonic stem cells of any kind and that his research data was fabricated . (newscientist.com)
  • In 1907 the zoologist Ross Granville Harrison demonstrated the growth of frog embryonic cells that would give rise to nerve cells in a medium of clotted lymph . (wikipedia.org)
  • It covers the biological nuts and bolts of one of the hottest fields in medical research: human embryonic stem cells. (consumerwatchdog.org)
  • WARF , in this and two other patents, in essence owns virtually all imaginable characteristics of human embryonic stem cells. (consumerwatchdog.org)
  • Basically, if it looks like an embryonic cell, you'd better pay up. (consumerwatchdog.org)
  • The fact that the DNA of a fully differentiated (adult) cell could be stimulated to revert to a condition comparable to that of a newly fertilized egg and to repeat the process of embryonic development demonstrates that all the genes in differentiated cells retain their functional capacity, although only a few are active. (who.int)
  • Yes, I worked with Gail Martin on embryonic stem cells and then returned to Harvard in 1986. (medscape.com)
  • The stage-specific embryonic antigen 4 (SSEA4) is com- isolate the NSCs from neonatal mice and rats (Campos monly used as a cell surface marker to identify the pluri- et al. (lu.se)
  • 2005). Notch1 and syndecan-1 potent human embryonic stem (ES) cells. (lu.se)
  • The researcher claimed he had created stem cell lines from cloned human embryos. (asianews.it)
  • In January 2006, Hwang's home research institution, Seoul National University, delivered a damning report about Hwang's work on cloned human embryos, concluding it was all based on fraudulent data. (nature.com)
  • Some prohibit only cloning for reproductive purposes and allow the creation of cloned human embryos for research, whereas others prohibit the creation of cloned embryos for any purpose. (who.int)
  • This is currently the most popular method for the generation of targeted knock-out and knock-in models by ES-cell injections into 8-cell morulae and/or blastocysts of mouse embryos. (lu.se)
  • shRNAs that generate siRNAs intracellularly are expressed from amphotropic lentivirus viral particles, allowing screening in a wide range of mammalian cell lines. (sigmaaldrich.com)
  • Selected clones will beused to generate high-yield producer stable mammalian cell lines for antibody production in large quantity. (sbir.gov)
  • Cloning, characterization, and expression of a human calcitonin receptor from an ovarian carcinoma cell line. (jci.org)
  • We have previously reported the establishment and preliminary characterization of polyclonal hepatitis B virus (HBV) nucleoprotein (HBcAg)-specific CD4+ and CD8+ T cell lines derived from the hepatic lymphomononuclear cell infiltrate of several patients with chronic active hepatitis B. The isolated subsets from these lines were specifically activated by HBcAg and displayed antigen-specific help and suppression with respect to proliferation of the alternate subset. (unipr.it)
  • basic characterization, including cell authentication (STR), undifferentiated state (FACS) and molecular karyotyping. (lu.se)
  • One of these lines was recently cloned by limiting dilution, and four HBcAg-specific CD3+ CD4+ CD8-DR+ T cell lines were produced that had a 95.3% likelihood of monoclonality. (unipr.it)
  • additionally, certain cell types do not survive the limited dilution step due to a need for secreted factors from neighboring cells. (mirusbio.com)
  • If limited dilution does not work, the cloning process might need to be carried out at higher cell concentrations and repeated a few times to ensure monoclonality. (mirusbio.com)
  • In this method, selected cells are seeded sparsely but not at limiting dilution in 10 cm dishes and allowed to expand and form discernible colonies for 2-3 weeks. (mirusbio.com)
  • Selection of edited cell clones currently relies on limiting dilution or fluorescence-activated cell sorting (FACS)-based single-cell sorting to isolate single cells. (nature.com)
  • The therapeutic potential of cloned human cells has been demonstrated by another study using human oocytes to reprogram adult cells of a type 1 diabetic. (news-medical.net)
  • With Hwang discredited, both the field of therapeutic cloning and the public's trust in science have suffered a serious setback. (nature.com)
  • In therapeutic cloning, the blastocyst is not transferred to a womb. (eurostemcell.org)
  • Another long-term hope for therapeutic cloning is that it could be used to generate cells that are genetically identical to a patient. (eurostemcell.org)
  • Therapeutic monoclonal antibodies (MAbs) mostly are manufactured using bioengineered mammalian cells cultured in a bioreactor for two to three weeks. (bioprocessintl.com)
  • NEW YORK, September 21, 2003 - New research from Memorial Sloan Kettering Cancer Center (MSKCC), Cornell University, and The University of Connecticut describes a novel way of producing therapeutic nerve cells that can cure mice with Parkinson's-like disease. (mskcc.org)
  • They're keen to use procedures such as therapeutic cloning to create ES cell lines based on genetic material collected from people with specific diseases. (consumerwatchdog.org)
  • After many divisions in culture, this single cell forms a blastocyst (an early stage embryo with about 100 cells) with almost identical DNA to the original donor who provided the adult cell - a genetic clone. (eurostemcell.org)
  • To produce Dolly, the cloned blastocyst was transferred into the womb of a recipient ewe, where it developed and when born quickly became the world's most famous lamb. (eurostemcell.org)
  • Therefore, the 1H91 clonal cell line may provide an in vitro model for studying the underlying cellular mechanisms of the type-1 astrocyte in reactive gliosis. (illinois.edu)
  • XIAP mRNA levels were established by RT-PCR, the four X (XIAP knockdown) clonal cell lines show 82-93% reduction in XIAP mRNA when compared to the four L (luciferase control) cell lines. (nih.gov)
  • Here, we establish a method to enable clonal isolation, selection, and expansion of properly edited cells, using OptoElectroPositioning technology for single-cell manipulation on a nanofluidic device. (nature.com)
  • Each colony is split and sequentially exported for on-target sequencing and further off-chip clonal expansion of the validated clones. (nature.com)
  • Each clonal line must then be assessed by sequencing to find those that bear the desired edits. (nature.com)
  • Generating validated clonal lines can require several weeks. (nature.com)
  • Cell lines are usually clonal, meaning that the entire population originated from a single common ancestor cell. (alliedmarketresearch.com)
  • Although many species produce clonal offspring in this fashion, Dolly, the lamb born in 1996 at a research institute in Scotland, was the first asexually produced mammalian clone. (who.int)
  • Myelodysplastic syndrome (MDS) in childhood encompasses a diverse group of bone marrow disorders that share a common clonal defect of stem cells and that result in ineffective hematopoiesis with dysplastic changes in the marrow. (medscape.com)
  • Transfection of this cDNA into COS cells resulted in expression of receptors with high affinity for salmon CT (Kd = approximately 0.44 nM) and for human CT (Kd = approximately 5.4 nM). (jci.org)
  • HPV-16 E6/E7 Stable Expression Hacat Cell Line Clone #11 - 1x106 cells / 1.0 ml - 1 kit is backordered and will ship as soon as it is back in stock. (phantome.org)
  • HPV-16 E6/E7 Stable Expression Hacat Cell Line Clone #11 - 1x106 ce. (phantome.org)
  • CD45RB expression appears to decrease as T lymphocytes progress from naive to memory cells. (bdbiosciences.com)
  • Ernst DN, Weigle WO, Noonan DJ, McQuitty DN, Hobbs MV. The age-associated increase in IFN-γ synthesis by mouse CD8+ T cells correlates with shifts in the frequencies of cell subsets defined by membrane CD44, CD45RB, 3G11, and MEL-14 expression. (bdbiosciences.com)
  • Here we use an expression-cloning strategy to identify SIV receptors and have isolated genes encoding two members of the seven-transmembrane G-protein-coupled receptor family that are used not only by SIVs, but also by strains of HIV-2 and M-tropic HIV-1. (nih.gov)
  • Commonly used cell lines include Chinese hamster ovary (CHO) mammalian cell expression systems, such as CHOK1. (iptonline.com)
  • This allows the biopharma industry to make real, substantive gains in cell selection control and achieve enhanced levels of expression. (iptonline.com)
  • When genome editing induces a phenotypic alteration that is detectable by fluorescence (i.e., cell-surface expression of a target that can be non-lethally assessed with fluorescently labeled antibody), FACS provides a method of enriching edited cells 9 , narrowing the number of clones to propagate and analyze. (nature.com)
  • The ectopic expression of human papilloma virus (HPV) E6/E7 gene has successfully been employed to establish these cell lines. (lu.se)
  • To induce differentiation and maturation of the immortalized cell lines, terminating the HPV-E6/E7 expression through a gene induction system has been believed to be essential. (lu.se)
  • Here, we report that erythroid cell lines established from human bone marrow using simple expression of HPV-E6/E7 are capable of normal erythroid differentiation, without turning gene expression off. (lu.se)
  • Upon differentiation, other ELLU clones shift from fetal to adult hemoglobin expression, giving rise to more mature cells. (lu.se)
  • At the same concentrations, they decreased the number of invading DU145 cells and increased the adherence of DU145 cells to fibronectin and collagen matrix at tested concentrations, accompanied by an increase in integrin ß-1 (10 µg/mL) and integrin ß-4 (2.5 µg/mL) expression. (bvsalud.org)
  • Alterations in CD44 expression were not homogeneous but rather depended on the extract and the cell line. (bvsalud.org)
  • immature cell is then subjected to negative selection to delete grouped based on their previously defined pheno- self-reactive cells before it leaves the BM to enter peripheral typic features, and a gene expression pattern for lymphoid organs, where it becomes a mature B cell [9]. (lu.se)
  • B lymphocyte development is a highly ordered process pro- expression analysis without the use of intermediate amplifica- ceeding from the progenitor cells in the bone marrow (BM) to tion steps. (lu.se)
  • To reduce the risk of analyzing cell line- pathway can be divided into distinct stages based on the specific features, we used several representative cell lines for recombination status of the Ig genes and on the expression each of four major stages in B cell development: pro-B, pre-B, pattern of surface markers and the presence of intracellular proteins [1- 6]. (lu.se)
  • The present results also suggest that one potential role of the MP6-derived thioredoxin could be the up regulation of IL-6 receptor expression in EBV-infected B cells. (lu.se)
  • Another example of artificial cloning is molecular cloning, a technique in molecular biology in which a single living cell is used to clone a large population of cells that contain identical DNA molecules. (wikipedia.org)
  • Molecular cloning refers to the process of making multiple molecules. (wikipedia.org)
  • However, a number of other features are needed, and a variety of specialised cloning vectors (small piece of DNA into which a foreign DNA fragment can be inserted) exist that allow protein production, affinity tagging, single-stranded RNA or DNA production and a host of other molecular biology tools. (wikipedia.org)
  • Dianzani U, Luqman M, Rojo J. Molecular associations on the T cell surface correlate with immunological memory. (bdbiosciences.com)
  • Despite improvements in the CRISPR molecular toolbox, identifying and purifying properly edited clones remains slow, laborious, and low-yield. (nature.com)
  • Identification of the Menkes gene by positional cloning has enabled molecular diagnosis of females who carry the gene and at-risk fetuses in certain families, enhancing preventive efforts. (medscape.com)
  • Mistletoe extracts do block bladder cancer growth in vitro, with the molecular action differing according to the cell line and the host tree of the mistletoe. (bvsalud.org)
  • 1990. Cell replication and unscheduled DNA synthesis (UDS) activity of low molecular weight chlorinated paraffins in the rat liver in vivo. (cdc.gov)
  • cell authentication (STR), pluripotency and undifferentiated state confirmation, molecular karyotype, g-banding and iPS growth pattern and morphology analysis. (lu.se)
  • Typically, most of the cells that have not integrated the transfected plasmid will die while the cells that have undergone plasmid integration will survive by 9 days post-transfection. (mirusbio.com)
  • This method can be employed if a detectable marker is expressed on the cells post-transfection. (mirusbio.com)
  • Unlike murine-based MMLV or MSCV retroviral systems, lentiviral-based particles permit efficient infection and integration of the specific shRNA construct into differentiated and non-dividing cells, such as neurons and dendritic cells, 1 overcoming low transfection and integration difficulties when using these cell lines. (sigmaaldrich.com)
  • Self-inactivating replication incompetent viral particles are produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. (sigmaaldrich.com)
  • Transfection, selection, and single-cell cloning are all part of the cell line generation process. (alliedmarketresearch.com)
  • The artificial cloning of organisms, sometimes known as reproductive cloning, is often accomplished via somatic-cell nuclear transfer (SCNT), a cloning method in which a viable embryo is created from a somatic cell and an egg cell. (wikipedia.org)
  • In 1996, Dolly the sheep achieved notoriety for being the first mammal cloned from a somatic cell. (wikipedia.org)
  • One cloning technology that has been developed for mammalian and human cells is somatic cell nuclear transfer (SCNT). (news-medical.net)
  • Cloning, or somatic cell nuclear transfer (SCNT), is the technique used to produce Dolly the sheep, the first animal to be produced as a genetic copy of another adult. (eurostemcell.org)
  • 체세포 핵 치환 (Somatic-cell nuclear transfer, SCNT)은 난자 의 핵 을 제거한 후에, 체세포 의 핵을 이식하여 복제 를 하는 기술을 말한다. (wikipedia.org)
  • Somatic-cell nuclear transfer, the technique by which Dolly was created, was first used 40 years ago in research with tadpoles and frogs. (who.int)
  • The nucleus of an adult somatic cell (such as a skin cell) is removed and transferred to an enucleated egg, which is then stimulated with electric current or chemicals to activate cell division. (who.int)
  • if it implants and the pregnancy goes to term, the resulting individual will carry the same nuclear genetic material as the donor of the adult somatic cell. (who.int)
  • Scientists were initially interested in somatic-cell nuclear transfer as a means of determining whether genes remain functional even after most of them have been switched off as the cells in a developing organism assume their specialized functions as blood cells, muscle cells, and so forth. (who.int)
  • This has led to a lot of interest in SCNT, which is best known as the method used to pioneer whole animal cloning technology, such as Dolly the sheep. (news-medical.net)
  • Seoul (AsiaNews) Seoul National University (SNU) yesterday suspended two colleagues of Hwang Woo-suk, a vet and university professor charged with faking his research results on human cloning. (asianews.it)
  • Lee Byeong-chun and Kang Seung-keun were accused of faking the outcome of studies on cloning human stem cells while working with Hwang. (asianews.it)
  • The South Korean stem-cell researcher Woo Suk Hwang has been at the centre of one of the largest investigations of scientific fraud in living memory. (nature.com)
  • Disgraced cloning expert Woo-Suk Hwang has admitted making donations to politicians using funds donated for his research, said South Korean state auditors on Monday. (newscientist.com)
  • Hwang became a national hero in South Korea after he claimed to have cloned the first human stem cell line in 2004, and stunned medical experts in 2005, claiming to have created 11 patient-specific human stem cell lines. (newscientist.com)
  • The committee said Hwang had forced two of his junior researchers to give eggs for his stem cell research, contrary to his earlier denials of forcing any of his staff to contribute eggs. (newscientist.com)
  • When the cloning process is used in this way, to produce a living duplicate of an existing animal, it is commonly called reproductive cloning. (eurostemcell.org)
  • In most countries, it is illegal to attempt reproductive cloning in humans. (eurostemcell.org)
  • Elaboration of an international convention against reproductive cloning of human beings has been under consideration in the United Nations since December 2001 when the subject was included in the agenda of the fifty- sixth session as a supplementary agenda item at the request of France and Germany. (who.int)
  • Occasionally, the term cloning is misleadingly used to refer to the identification of the chromosomal location of a gene associated with a particular phenotype of interest, such as in positional cloning. (wikipedia.org)
  • Cloning is the process of producing individual organisms with identical genomes, either by natural or artificial means. (wikipedia.org)
  • In sexual reproduction, clones are created when a fertilized egg splits to produce identical (monozygous) twins with identical genomes. (who.int)
  • Moreover, treatment with imiquimod downregulated MHC class I molecules on tumor cells possibly resulting in a reduced binding affinity for inhibitory receptor NKG2A expressed on γδ T cells. (aacrjournals.org)
  • have combined two microscopy techniques called 'single-particle tracking' and 'photoactivated localization microscopy' to track how individual molecules of activated Ras move in human cells grown in the lab. (elifesciences.org)
  • From there, the cell constantly removed Ras molecules from these membrane domains and returned them back to their 'fast' diffusing state. (elifesciences.org)
  • The term applies not only to entire organisms but also to copies of molecules (such as DNA) and cells. (who.int)
  • But SCNT can also be used to clone human cells for transplant or other therapies. (news-medical.net)
  • Another successful attempt at human SCNT was made using cells from two adult males. (news-medical.net)
  • These stem cells are genetically matched to the donor organism, holding promise for studying genetic disease. (eurostemcell.org)
  • In 2001, Lorenz Studer, MD , Head of the Stem Cell and Tumor Biology Laboratory at MSKCC, and his colleagues at Rockefeller University published research in which they generated unlimited numbers of genetically matched dopamine nerve cells using cloned stem cells whose genetic material originated from the mouse's own tail. (mskcc.org)
  • The Vero cell line was initiated from the kidney of a normal adult African Green monkey. (topsan.org)
  • In this procedure, the nucleus of an egg cell is removed and replaced by the nucleus of a cell from another adult. (eurostemcell.org)
  • In Dolly's case, the cell came from the mammary gland of an adult ewe. (eurostemcell.org)
  • After being inserted into the egg, the adult cell nucleus is reprogrammed by the host cell. (eurostemcell.org)
  • For example, stem cells could be generated using the nuclear transfer process described above, with the donor adult cell coming from a patient with diabetes or Alzheimer's. (eurostemcell.org)
  • ELLU is heterogeneous and, unexpectedly, clones expressing adult hemoglobin rapidly differentiate and produce fragile cells. (lu.se)
  • A) Sex differences in density of -gal+ (PR+) cells in adult PRPL/PL mice. (cdc.gov)
  • The authors conclude that the Alamar- blue assay employing the NCTC-clone-929 subclone A12 is effective in the quantitation of low levels of TNF, particularly when a suitable immunoassay is unavailable. (cdc.gov)
  • Cell growth was assessed by a well-characterized MTT assay, while BrdU and clonogenicity assays provided information on the cell proliferation index. (bvsalud.org)
  • 1983) and the multipotent progenitor cells from fetal disease (Bjorklund and Lindvall, 2000). (lu.se)
  • General Assembly the following year,3 and the World Medical Association's Resolution on Cloning, endorsed in 1997, have confronted the issue but lack binding legal force. (who.int)
  • Progress has been made to enhance the efficiency of HDR-based editing 8 , however, a technology to identify cells with desired mono- or bi-allelic edits is urgently needed to realize the full potential of CRISPR. (nature.com)
  • if ordering 3 different clones, and 1 vial of each clone, the total quantity of Additional Clones would be 2). (mmrrc.org)
  • Furthermore, in order to clarify whether these 3 kinds of cells originate from one stem cell or not, monoclonal cell population were transplanted into nude mice, and its histological investigation revealed that 3 types of cells existed in the transplanted tumor, proving that they originate from one stem cell. (elsevierpure.com)
  • HIV persistence may arise from ongoing residual virus replication and/or from latently-infected cells defined as the cellular reservoir in which long-lived resting memory CD4+ T cells harbouring an integrated but transcriptionally silent provirus represent the largest pool in the blood (Chomont et al. (europa.eu)
  • The effect of bone marrow stromal cells appeared to be caused by a diffusable factor(s), but close cellular interaction could not be excluded since cloning in microwells produced consistent and optimal cell growth compared to growth in soft agar. (edu.au)
  • Cellular differentiation of this stem cell type occurs when hormonal precursors are added to the medium. (sigmaaldrich.com)
  • The data indicated that both cell lines, either together or alone, may be useful as the cellular substrate for an artificial liver device. (usda.gov)
  • No differentiation and self-organization into multi-cellular bile ductules, as observed in the parental PICM-19 cell line, occurred within the PICM-19H cell monolayer even after six months of maintenance culture. (usda.gov)
  • These data indicated that both cell lines, either together or alone, may be useful as the cellular substrate for an artificial liver device. (usda.gov)
  • Our findings propose that it is not necessary to employ gene induction systems to establish immortalized erythroid cell lines sustaining differentiation potential and describe novel cellular characteristics for desired functionally competent clones. (lu.se)
  • We have here examined various cell-derived cytokines for their ability to enhance both the cellular outgrowth of newly infected B cells and the clonability of infected B cells and lymphoblastoid cell lines. (lu.se)
  • The 16A monoclonal antibody specifically recognizes an exon B-dependent epitope of CD45 lycoprotein, which is found at high density on peripheral B cells, T cytotoxic/suppressor cells, a subset of T helper cells, and most thymocytes, and at low density on macrophages and dendritic cells. (bdbiosciences.com)
  • Bottomly K, Luqman M, Greenbaum L. A monoclonal antibody to murine CD45R distinguishes CD4 T cell populations that produce different cytokines. (bdbiosciences.com)
  • However, because of low transformability, low clonability, and the inherent instability of EBV-infected B cells, valuable antibody-producing B cells are often lost during this procedure. (lu.se)
  • All cloned lines were interleukin 2 dependent, produced interferon-gamma in an antigen-specific manner, and provided antigen-specific help to autologous B cells with respect to anti-HBc production. (unipr.it)
  • We conclude that HBcAg-specific, HLA-class II restricted helper T cells capable of inducing antigen-specific functional responses by autologous B lymphocytes and T lymphocytes are present at the site of viral antigen synthesis and hepatocellular injury in HBV infection. (unipr.it)
  • The CD45 isoforms play complex roles in T-cell and B-cell antigen receptor signal transduction. (bdbiosciences.com)
  • Interacts with T-cell surface antigen CD2. (lu.se)
  • An improved tumor necrosis factor (TNF) bioassay using an isolated subclone from the murine fibroblastoid NCTC-clone-929 cell line and a new fluorescence indicator system for detecting target cell viability was described. (cdc.gov)
  • The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture . (wikipedia.org)
  • Since Haberlandt's original assertions, methods for tissue and cell culture have been realized, leading to significant discoveries in biology and medicine. (wikipedia.org)
  • The present report gives an overview of the terms and methods used in cloning and summarizes the debates in the General Assembly. (who.int)
  • fication and isolation via fluorescence-activated cell sort- ing (FACS). (lu.se)
  • Two new cell lines, PICM-19H and PICM-19B, were derived from the parental PICM-19 pig liver stem cell line. (usda.gov)
  • Two cell lines, PICM-19H and PICM-19B, were derived from the bipotent PICM-19 pig liver stem cell line and assessed for their potential application in artificial liver devices. (usda.gov)
  • depictions commonly involve themes related to identity, the recreation of historical figures or extinct species, or cloning for exploitation (i.e. cloning soldiers for warfare). (wikipedia.org)
  • Cloning is commonly used to amplify DNA fragments containing whole genes, but it can also be used to amplify any DNA sequence such as promoters, non-coding sequences and randomly fragmented DNA. (wikipedia.org)
  • Tissue culture commonly refers to the culture of animal cells and tissues, with the more specific term plant tissue culture being used for plants. (wikipedia.org)
  • The cloned cDNA encodes a putative peptide of 490 amino acids with seven potential transmembrane domains. (jci.org)
  • We have established a permanent cell line (1H91) of putative type-1 astrocyte precursor cells that were clonally derived from a single cell isolated from E16 mouse cerebellum. (illinois.edu)
  • Most natural cloning occurs in those species that produce their descendants asexually, that is, without combining the male and female genetic material. (who.int)
  • Using cloning rings and/or trypsin discs is a viable option for adherent cells in this scenario. (mirusbio.com)
  • Cell culture techniques were advanced significantly in the 1940s and 1950s to support research in virology . (wikipedia.org)
  • Eigenmann, PA, Huang, SK, Ho, DG & Sampson, HA 1996, ' Human T cell clones and cell lines specific to ovomucoid recognize different domains and consistently express IL-5 ', Advances in experimental medicine and biology , vol. 409. (johnshopkins.edu)
  • Our scientists pursue every aspect of cancer research-from exploring the biology of genes and cells, to developing immune-based treatments, uncovering the causes of metastasis, and more. (mskcc.org)
  • The cloning of established human leukemia and lymphoma cell lines at limiting dilutions in microwells and soft agar is described. (edu.au)
  • In general, the cloning efficiency in microwells was greater than in soft agar. (edu.au)
  • abstract = "A cell line, designated RMG-I, has been established from ascites of a patient with ovarian clear cell carcinoma. (elsevierpure.com)
  • If artificial cloning and natural cloning both lead to the same result, which is the formation of a clone, that is, an organism with identical or nearly identical genes to another organism, then the plight of This creation is very different between the two creatures. (wikipedia.org)
  • The MISSION product line is based on a viral vector-based RNAi library against annotated mouse and human genes. (sigmaaldrich.com)
  • Subsequent differentiation allows for rear- analysis of a set of mouse B lineage cell lines rep- rangements of the Ig light-chain (IgL) genes that replace the resenting defined stages of B cell development us- surrogate light-chain genes on the surface of the B cell [8]. (lu.se)
  • Within this work, and considering the discussed background, another D. tertiolecta clone (CCMP 1320) was selected and tested against several cancer cell lines, and a bioactivity-guided fractionation approach allowed to identify the source of the biological activity observed within the extracts of this green alga. (frontiersin.org)
  • There are no in-vitro tests to identify clone quality. (lu.se)
  • Growth of most cell lines was improved by the addition of human AB serum and irradiated human bone marrow stromal cells. (edu.au)
  • Myelodysplastic syndrome refers to a group of related disorders in which abnormal blood-forming cells develop in the bone marrow. (msdmanuals.com)
  • White blood cells develop from stem cells in the bone marrow. (msdmanuals.com)
  • In myelodysplastic syndromes, a line of identical cells (clone) develops and occupies the bone marrow. (msdmanuals.com)
  • Bone Marrow Examination Red blood cells, most white blood cells, and platelets are produced in the bone marrow, the soft fatty tissue inside bone cavities. (msdmanuals.com)
  • Natural cloning occurs through a variety of natural mechanisms, from single-celled organisms to complex multicellular organisms. (wikipedia.org)
  • As a result, biopharma CDMO leaders have gravitated to a platform that efficiently produces robust and stable cell lines. (iptonline.com)
  • Media and feed have been optimised to support both the initial cell-line engineering process and stable cell-line selection through to large-scale growth and production. (iptonline.com)
  • Cell line development is the process of creating stable cell lines for use in a variety of applications, including the creation of biologics (recombinant proteins and monoclonal antibodies), drug screening, and gene functional investigations. (alliedmarketresearch.com)
  • For instance, in April 2020, CEVEC Pharmaceuticals GmbH announced the launch of the ELEVECTA platform, which will produce stable cell lines for AAV (Adeno-associated viral) vector for gene therapy. (alliedmarketresearch.com)
  • It can also cause TAC in other conditions in which increased red cell production is necessary to maintain stable red cell indices, as may occur in anemia due to blood loss. (cdc.gov)
  • In the field of biotechnology, cloning is the process of creating cloned organisms of cells and of DNA fragments. (wikipedia.org)
  • The work, which will be published in the October issue of Nature Biotechnology (available online September 21), provides the first evidence that cloned cells can cure disease in an animal model. (mskcc.org)
  • The majority of EGF-treated 1H91 cells are not immunoreactive for glial acid fibrillary protein (GFAP). (illinois.edu)
  • Immunoblot analysis showed a 67-89% reduction in XIAP protein in X cell lines compared to L. RNA was analysed by microarray and XIAP knockdown was confirmed in 7 probe sets, there was no significant compensation of other IAP family members. (nih.gov)
  • Through mRNA administration, vaccines prompt your cells to produce a harmless version of the spike protein, thereby stimulating your immune system to produce antibodies to fight off the potential threat. (moleculardevices.com)
  • In SARS-CoV-2, the aim is to disrupt the binding of the spike protein with ACE2 receptors, prohibiting the entry of the virus into the host cells. (moleculardevices.com)
  • Blocking and knock-down studies served to correlate protein alterations with cell growth. (bvsalud.org)
  • Red blood cells contain hemoglobin, a protein that enables them to carry oxygen from the lungs and deliver it to all parts. (msdmanuals.com)
  • Involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase. (lu.se)
  • Three-dimensional solution structure of the extracellular region of the complement regulatory protein CD59, a new cell-surface protein domain related to snake venom neurotoxins. (lu.se)
  • Desirable attributes of a CHO cell line include its ability to produce high titers of biotherapeutic proteins facilitate quick recoveries and selection processes maintain phenotypic and genetic stability throughout in-vitro aging of a culture. (bioprocessintl.com)
  • Six parasites ont montré une résistance in vitro au stibogluconate de sodium en utilisant le test de détection des amastigotes dans les macrophages J774 murins. (who.int)
  • Bioprocessing begins upstream, most often with culturing of animal or microbial cells in a range of vessel types (such as bags or stirred tanks) using different controlled feeding, aerating, and process strategies. (bioprocessintl.com)
  • Deliver 2 clonally expanded mycoplasma free iPSC lines (15 x 300k cells). (lu.se)
  • To optimize the chances of achieving germline transmission, investigators are encouraged to order more than one clone. (mmrrc.org)
  • Although good quality ES cells can provide chimeras, we do not guarantee germline transmission. (lu.se)
  • Mice derived from requested cell lines via Microinjection services will be pathogen tested prior to delivery of mice and health reports will be provided. (mmrrc.org)
  • F) The vast majority of -gal+ (PR+) cells in various regions in PRPL mice are neurons, as evidenced by co-labeling with NeuroTrace (fluorescent Nissl) in the arcuate and NeuN for the remaining regions. (cdc.gov)
  • G) No sex difference in nuclear or soma size of PR+ VMHvl cells in PRPL mice. (cdc.gov)