• proteins
  • Shark antibodies and their human equivalents are small and extremely stable with long loops that are able to penetrate into important regions of proteins. (edu.au)
  • Our group uses a library of shark antibodies and a library of the human version of these antibodies to identify molecules that will bind to proteins that have been shown to be involved in human diseases. (edu.au)
  • 3) Antibodies can also be generated that bind cell surface major histocompatibility complex class I (MHC-I)-presented peptides that are derived from intracellular proteins. (frontiersin.org)
  • In this study, we describe an effective protocol for use in a multiplexed high-throughput antibody microarray with glycan binding protein detection that allows for the glycosylation profiling of specific proteins. (jove.com)
  • Through the use of an antibody panel and probing with multiple biotinylated lectins, this method allows for an effective glycosylation profile of the different proteins found in a given human or animal sample to be developed. (jove.com)
  • Specific examples of this work include the automatic modeling of antibody variable domains, the general modeling of homologous proteins and studies of DNA base-pair mismatches. (stanford.edu)
  • The traditional approaches that individual and a panel of cancer biomarkers are selected by analytic methods such as analysis of variance (ANOVA), Lasso, pairwise, information theory and support vector machine (SVM) do not explicitly consider interaction between genes, proteins and metabolites. (jcancer.org)
  • However, non-antibody proteins that can be engineered to bind such targets are also of high interest in the biopharmaceutical industry. (patentsencyclopedia.com)
  • These "alternative scaffold" proteins may have advantages over traditional antibodies due to their small size, lack of disulphide bonds, high stability, and ability to be expressed in prokaryotic hosts. (patentsencyclopedia.com)
  • This fold is found in the variable regions of antibodies, as well as thousands of non-antibody proteins. (patentsencyclopedia.com)
  • Assays
  • The advancement of high throughput omic technologies during the past few years has made it possible to perform many complex assays in a much shorter time than the traditional approaches. (jcancer.org)
  • molecular
  • With the development of molecular cloning technology and the deep understanding of antibody engineering, there are diverse bispecific antibody formats from which to choose to pursue the optimal biological activity and clinical purpose. (mdpi.com)
  • Studies on single gene/protein/metabolite molecular signatures offer limited insight into the complex interplay among the molecules responsible for progression of complex diseases such as cancer. (jcancer.org)
  • amino
  • Interestingly, the same region of NS1, which comprises amino acids 224HWPKPHTLW232, was conserved for both kinds of antibodies displaying the consensus motif histidine-tryptophan-tryptophan or tryptophan-proline-tryptophan. (jove.com)
  • Questions we are trying to answer include: How can a protein be stabilized by a single amino acid change? (stanford.edu)
  • peptide
  • These tumor-associated peptide-MHC-I complexes can then be targeted by antibodies known as T-cell receptor mimic (TCRm) or T-cell receptor (TCR)-like antibodies, which recognize epitopes comprising both the peptide and the MHC-I molecule, similar to the recognition of such complexes by the TCR on T cells. (frontiersin.org)
  • genes
  • After successful expression of DENV type 2 NS1 protein, the genes of VHH antibodies against NS1 protein were biopanned from a non-immune llama library by phage display. (jove.com)
  • protein
  • The new protocol described in this study makes use of a chemically blocked antibody microarray with glycan-binding protein (GBP) detection and significantly reduces the time, cost, and lab equipment requirements needed to study protein glycosylation. (jove.com)
  • has recently invented a chemically blocked antibody microarray with a glycan-binding protein (GBP) detection method for high-throughput and multiplexed profile glycosylation of native glycoproteins in a complex sample 18 . (jove.com)
  • overcome
  • Bispecific antibodies that can inhibit two correlated signaling molecules at the same time can be developed to overcome inherent or acquired resistance and to be more efficient angiogenesis inhibitors. (mdpi.com)
  • Biosensors have shown tremendous promise to overcome these limitations and are being aggressively studied to provide rapid, reliable and sensitive detection platforms for such applications. (mdpi.com)
  • target
  • Antibodies have been developed to target externalized antigens, have also been engineered to enter into cells or may be expressed intracellularly with the aim of binding intracellular antigens. (frontiersin.org)
  • 1) It is possible for antibodies (or their derivatives) to target antigens that are normally intracellular but become externalized (for example, during disease). (frontiersin.org)
  • methods
  • Compared to traditional methods, pathway and network centric methods naturally provide a way to understand the underlying pathways and the interactions between individual signature markers and non-markers. (jcancer.org)
  • development
  • Our single domain antibody against CXCR4 was found to block HIV invasion as well as being able to reduce inflammation and stop the development of fibrosis in mouse models of lung fibrosis and eye fibrosis. (edu.au)
  • broad
  • Bispecific antibodies also have broad application prospects in bone disorders and infections and diseases of the central nervous system. (mdpi.com)
  • complex
  • The blocked, immobilized glycoprotein-specific antibodies are able to capture and isolate glycoproteins from a complex sample that is applied directly onto the microarray slides. (jove.com)
  • rapid
  • The impressive and rapid progress in micro- and nanotechnology as well as in biotechnology enables the integration of a variety of analytical functions in a single chip. (mdpi.com)
  • cells
  • The Vibrio cholerae T6SS can remodel the cytoskeleton of eukaryotic host cells by injecting an "evolved" VgrG carrying a C-terminal actin cross-linking domain 6,7 . (jove.com)
  • Thus
  • This leads to a wide concept for the construction of diverse bispecific antibodies, and thus, a large number of technologies for constructing bispecific antibodies have emerged. (mdpi.com)
  • include
  • Bispecific antibodies in the IgG format include asymmetric bispecific antibodies and homodimerized bispecific antibodies, all of which have an extended blood half-life and their own crystalline fragment (Fc)-mediated functions. (mdpi.com)