• proteins
  • Shark antibodies and their human equivalents are small and extremely stable with long loops that are able to penetrate into important regions of proteins. (edu.au)
  • Our group uses a library of shark antibodies and a library of the human version of these antibodies to identify molecules that will bind to proteins that have been shown to be involved in human diseases. (edu.au)
  • In this study, we describe an effective protocol for use in a multiplexed high-throughput antibody microarray with glycan binding protein detection that allows for the glycosylation profiling of specific proteins. (jove.com)
  • Through the use of an antibody panel and probing with multiple biotinylated lectins, this method allows for an effective glycosylation profile of the different proteins found in a given human or animal sample to be developed. (jove.com)
  • Specific examples of this work include the automatic modeling of antibody variable domains, the general modeling of homologous proteins and studies of DNA base-pair mismatches. (stanford.edu)
  • However, non-antibody proteins that can be engineered to bind such targets are also of high interest in the biopharmaceutical industry. (patentsencyclopedia.com)
  • This fold is found in the variable regions of antibodies, as well as thousands of non-antibody proteins. (patentsencyclopedia.com)
  • A second adhesive alignment forms when the interdigitated proteins separate by 70 +/- 10 A. A third complex forms at a bilayer separation commensurate with the approximate overlap of cadherin extracellular domains 1 and 2 (CEC1-2). (embl.de)
  • Sequence
  • The main advantage of this method is that it is not necessary to know all or almost all residues in a sequence as required for other traditional classification tools such as BLAST, FASTA, and HMM. (embl.de)
  • amino
  • Interestingly, the same region of NS1, which comprises amino acids 224HWPKPHTLW232, was conserved for both kinds of antibodies displaying the consensus motif histidine-tryptophan-tryptophan or tryptophan-proline-tryptophan. (jove.com)
  • Questions we are trying to answer include: How can a protein be stabilized by a single amino acid change? (stanford.edu)
  • targets
  • In addition we are collaborating with various groups to identify other single domains from our libraries against other targets of interest including Amyloid protein, which is involved in Alzheimer's disease. (edu.au)
  • sequences
  • In addition, we proposed a model for the secondary structure of the cytoplasmic domain of cadherins based on the principal relations between sequences and secondary structure multialignments. (embl.de)
  • protein
  • After successful expression of DENV type 2 NS1 protein, the genes of VHH antibodies against NS1 protein were biopanned from a non-immune llama library by phage display. (jove.com)
  • The new protocol described in this study makes use of a chemically blocked antibody microarray with glycan-binding protein (GBP) detection and significantly reduces the time, cost, and lab equipment requirements needed to study protein glycosylation. (jove.com)
  • has recently invented a chemically blocked antibody microarray with a glycan-binding protein (GBP) detection method for high-throughput and multiplexed profile glycosylation of native glycoproteins in a complex sample 18 . (jove.com)
  • overcome
  • Bispecific antibodies that can inhibit two correlated signaling molecules at the same time can be developed to overcome inherent or acquired resistance and to be more efficient angiogenesis inhibitors. (mdpi.com)
  • Biosensors have shown tremendous promise to overcome these limitations and are being aggressively studied to provide rapid, reliable and sensitive detection platforms for such applications. (mdpi.com)
  • molecular
  • With the development of molecular cloning technology and the deep understanding of antibody engineering, there are diverse bispecific antibody formats from which to choose to pursue the optimal biological activity and clinical purpose. (mdpi.com)
  • characteristics
  • The patterns of the secondary structure of this domain can serve as the distinguishing characteristics of cadherins. (embl.de)
  • orientation
  • The proper orientation of these domains is mediated by their hydrophobic interface and is stabilized by their linkage to disulfide-linked constant domains (CH1 and CL). (readbyqxmd.com)
  • development
  • Our single domain antibody against CXCR4 was found to block HIV invasion as well as being able to reduce inflammation and stop the development of fibrosis in mouse models of lung fibrosis and eye fibrosis. (edu.au)
  • complex
  • The blocked, immobilized glycoprotein-specific antibodies are able to capture and isolate glycoproteins from a complex sample that is applied directly onto the microarray slides. (jove.com)
  • Thus
  • This leads to a wide concept for the construction of diverse bispecific antibodies, and thus, a large number of technologies for constructing bispecific antibodies have emerged. (mdpi.com)
  • variety
  • The impressive and rapid progress in micro- and nanotechnology as well as in biotechnology enables the integration of a variety of analytical functions in a single chip. (mdpi.com)
  • multiple
  • In this method, multiple immobilized glycoprotein-specific antibodies are printed directly onto the microarray slides and the N-glycans on the antibodies are blocked. (jove.com)