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  • enzymology
  • In enzymology, an ubiquitin-protein ligase (EC 6.3.2.19) is an enzyme that catalyzes the chemical reaction ATP + ubiquitin + protein lysine ⇌ {\displaystyle \rightleftharpoons } AMP + diphosphate + protein N-ubiquityllysine The 3 substrates of this enzyme are ATP, ubiquitin, and a lysine residue on a protein, whereas its 3 products are AMP, diphosphate, and protein N-ubiquityllysine. (wikipedia.org)
  • In enzymology, a tubulin-tyrosine ligase (EC 6.3.2.25) is an enzyme that catalyzes the chemical reaction ATP + detyrosinated alpha-tubulin + L-tyrosine ⇌ {\displaystyle \rightleftharpoons } alpha-tubulin + ADP + phosphate The 3 substrates of this enzyme are ATP, detyrosinated alpha-tubulin, and L-tyrosine, whereas its 3 products are alpha-tubulin, ADP, and phosphate. (wikipedia.org)
  • In enzymology, an imidazoleacetate-phosphoribosyldiphosphate ligase (EC 6.3.4.8) is an enzyme that catalyzes the chemical reaction ATP + imidazole-4-acetate + 5-phosphoribosyl diphosphate ⇌ {\displaystyle \rightleftharpoons } ADP + phosphate + 1-(5-phosphoribosyl)imidazole-4-acetate + diphosphate The 3 substrates of this enzyme are ATP, imidazole-4-acetate, and 5-phosphoribosyl diphosphate, whereas its 4 products are ADP, phosphate, 1-(5-phosphoribosyl)imidazole-4-acetate, and diphosphate. (wikipedia.org)
  • In enzymology, phosphoribosylamine-glycine ligase, also known as glycinamide ribonucleotide synthetase (GARS), (EC 6.3.4.13) is an enzyme that catalyzes the chemical reaction ATP + 5-phospho-D-ribosylamine + glycine ⇌ {\displaystyle \rightleftharpoons } ADP + phosphate + N1-(5-phospho-D-ribosyl)glycinamide which is the second step in purine biosynthesis. (wikipedia.org)
  • PMID
  • Then, in a second, "editing" step, the ligase itself rapidly hydrolyses only the valylated products [ PMID: 9554847 , PMID: 10446055 ] as shown from the crystal structures. (ebi.ac.uk)
  • Enzymes
  • ligase Any of a class of enzymes that catalyse the formation of covalent bonds using the energy released by the cleavage of ATP. (encyclopedia.com)
  • In biochemistry, a ligase is an enzyme that can catalyze the joining of two large molecules by forming a new chemical bond, usually with accompanying hydrolysis of a small pendant chemical group on one of the larger molecules or the enzyme catalyzing the linking together of two compounds, e.g., enzymes that catalyze joining of C-O, C-S, C-N, etc. (wikipedia.org)
  • Ligases are classified as EC 6 in the EC number classification of enzymes. (wikipedia.org)
  • Human genes encoding formate-tetrahydrofolate ligases include: MTHFD1 - cytoplasmic MTHFD1L - mitochondrial As of late 2007, 3 structures have been solved for this class of enzymes, with PDB accession codes 1EG7, 1FP7, and 1FPM. (wikipedia.org)
  • Gene
  • Kletzin A. Molecular characterisation of a DNA ligase gene of the extremely thermophilic archaeon Desulfurolobus ambivalens shows close phylogenetic relationship to eukaryotic ligases. (ebi.ac.uk)
  • Purified DNA ligase is used in gene cloning to join DNA molecules together to form recombinant DNA. (wikipedia.org)
  • It is formed by alternative splicing of a proteolytic fragment of DNA ligase III and does not have its own gene, therefore it is often considered to be virtually identical to DNA ligase III. (wikipedia.org)
  • In yeast, GARS is part of a bifunctional enzyme (encoded by the ADE5/7 gene) in conjunction with phosphoribosylformylglycinamidine cyclo-ligase (AIRS). (wikipedia.org)
  • fragments
  • The common names of ligases often include the word "ligase", such as DNA ligase, an enzyme commonly used in molecular biology laboratories to join together DNA fragments. (wikipedia.org)
  • DNA ligase I: ligates the nascent DNA of the lagging strand after the Ribonuclease H has removed the RNA primer from the Okazaki fragments. (wikipedia.org)
  • The first catalytic RNAs produced by in vitro evolution were RNA ligases, catalytic RNAs that join two RNA fragments to produce a single adduct. (wikipedia.org)
  • All these ligases catalyze the formation of a 3'-5' phosphodiester bond between two RNA fragments. (wikipedia.org)
  • A mismatch at the 3′ end of the discriminating primer prevents the DNA ligase from joining the two fragments together. (wikipedia.org)
  • molecules
  • Ligases are important in the synthesis and repair of many biological molecules, including DNA (see DNA ligase ), and are used in genetic engineering to insert foreign DNA into cloning vectors . (encyclopedia.com)
  • Parkin is an E3 ubiquitin ligase that plays a critical role in ubiquitination- the process whereby molecules are covalently labelled with ubiquitin (Ub) and directed towards degradation in proteasomes or lysosomes. (wikipedia.org)
  • vitro
  • The RNA Ligase ribozyme was the first of several types of synthetic ribozymes produced by in vitro evolution and selection techniques. (wikipedia.org)
  • Okazaki
  • Secara biologis, DNA ligase diperlukan untuk menggabungkan fragmen Okazaki saat proses replikasi, menyambung potongan-potongan DNA yang baru disintesis, serta berperan dalam proses reparasi DNA. (wikipedia.org)
  • ligate
  • Does anyone have any experience with using T4 RNA ligase to ligate a single stranded DNA leader onto single stranded DNA? (bio.net)
  • Ubiquitination
  • Ubiquitination by E3 ligases regulates diverse areas such as cell trafficking, DNA repair, and signaling and is of profound importance in cell biology. (wikipedia.org)
  • proteins
  • one successful approach, for example, tested T4 DNA ligase fused to several alternative DNA binding proteins and found that the constructs with either p50 or NF-kB as fusion partners were over 160% more active in blunt-end ligations for cloning purposes than wild type T4 DNA ligase. (wikipedia.org)
  • E3 ligases are also key players in cell cycle control, mediating the degradation of cyclins, as well as cyclin dependent kinase inhibitor proteins. (wikipedia.org)
  • phosphodiester bond
  • DNA ligase is a specific type of enzyme, a ligase, (EC 6.5.1.1) that facilitates the joining of DNA strands together by catalyzing the formation of a phosphodiester bond. (wikipedia.org)
  • Single-strand breaks are repaired by DNA ligase using the complementary strand of the double helix as a template, with DNA ligase creating the final phosphodiester bond to fully repair the DNA. (wikipedia.org)
  • DNA ligase in E. coli, as well as most prokaryotes, uses energy gained by cleaving nicotinamide adenine dinucleotide (NAD) to create the phosphodiester bond. (wikipedia.org)
  • complementary
  • It plays a role in repairing single-strand breaks in duplex DNA in living organisms, but some forms (such as DNA ligase IV) may specifically repair double-strand breaks (i.e. a break in both complementary strands of DNA). (wikipedia.org)
  • residue
  • The co-factor ATP is cleaved to pyrophosphate and AMP, with the AMP being covalently joined to a highly conserved lysine residue in the active site of the ligase. (ebi.ac.uk)
  • activity
  • T4 ligase, AFAIK, does not have nuclease activity. (bio.net)
  • There are at least three different units used to measure the activity of DNA ligase: Weiss unit - the amount of ligase that catalyzes the exchange of 1 nmole of 32P from inorganic pyrophosphate to ATP in 20 minutes at 37°C. This is the one most commonly used. (wikipedia.org)
  • ligation
  • used anyone the T4 RNA ligase for ssDNA / oligonucleotide-ligation? (bio.net)
  • Regulation of inter‐ and intramolecular ligation with T4 DNA ligase in the presence of polyethylene glycol. (currentprotocols.com)
  • Polymer‐stimulated ligation: Enhanced blunt‐ or cohesive‐end ligation of DNA or deoxyribooligonucleotides by T4 DNA ligase in polymer solutions. (currentprotocols.com)
  • Its half-life is 48 hours at 65°C and greater than 1 hour at 95°C. Ampligase DNA Ligase has been shown to be active for at least 500 thermal cycles (94°C/80°C) or 16 hours of cycling.10 This exceptional thermostability permits extremely high hybridization stringency and ligation specificity. (wikipedia.org)
  • eubacteria
  • There are two forms of DNA ligase, one requires ATP ( EC:6.5.1.1 ), the other NAD ( EC:6.5.1.2 ), the latter being restricted to eubacteria. (ebi.ac.uk)
  • family
  • This family includes UDP-N-acetylmuramate-L-alanine ligase (MurC), UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase (MurE), and UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase (MurF). (wikipedia.org)
  • Like other members of the RING-between-RING (RBR) family of E3 ligases, parkin possesses two RING finger domains and an in-between-RING (IBR) region. (wikipedia.org)
  • nick
  • DNA ligase merupakan enzim yang mengkatalisis pembentukan ikatan fosfodiester antara ujung 5'-fosfat dan 3'-hidroksil pada DNA yang mengalami nick . (wikipedia.org)
  • known
  • Of the all known mammalian DNA ligases, only Lig III has been found to be present in mitochondria. (wikipedia.org)
  • The most active ligase known to date is the Class I ligase, isolated from random sequence (work of David Bartel, while in the Szostak lab). (wikipedia.org)