• Immobilized sugars and sugar derivatives offer an effective means of purification for a variety of proteins, which recognize and bind carbohydrate moieties. (sigmaaldrich.com)
  • Dye ligand chromatography is affinity chromatography that utilizes covalently bond textile dye (reactive dyes) to purify proteins. (sigmaaldrich.com)
  • The amino acid sequence of human CD14 is approximately 65% identical with the mouse, rat, rabbit, and bovine proteins. (bio-techne.com)
  • Probe Set ID Ref Seq Protein ID Signal Strength Name Gene Symbol Species Function Swiss-Prot ID Amino Acid Sequence 1367452_at NP_598278 16.8 small ubiquitin-related modifier 2 precursor Sumo2 Rattus norvegicus " Ubiquitin-like protein that can be covalently attached to proteins as a monomer or as a lysine-linked polymer. (nih.gov)
  • Aptamers are short sequences of artificial DNA or RNA that bind to specific target molecules such as peptides, proteins, carbohydrates, toxins, small molecules or live cells. (statesacademicpress.com)
  • This gene encodes a member of the C-lectin family, proteins that possess collagen-like sequences and carbohydrate recognition domains. (nih.gov)
  • C-type lectins (CTLs) constitute the largest and most diverse super family of proteins that share a characteristic carbohydrate recognition domain (CRD) with two or three pairs of disulfide bonds [ 1 , 2 ]. (biomedcentral.com)
  • The basic strategy for solving 3-dimensional structures of larger proteins and protein-ligand complexes in solution using 3- and 4-dimensional NMR spectroscopy is summarized, and the power of these methods is illustrated using 3 examples: interleukin-1 beta, the complex of calmodulin with a target peptide, and the specific complex of the transcription factor GATA-1 with its cognate DNA target site. (nih.gov)
  • The receptor-binding domain of toxin A is larger than the equivalent domain on toxin B and, according to structure prediction, binds through a multivalent mechanism to seven carbohydrate ligands, whereas the toxin B would bind to four carbohydrate ligands. (medscape.com)
  • CD14 is a pattern recognition receptor that binds Lipopolysaccharides (LPS) and a variety of ligands derived from different microbial sources. (bio-techne.com)
  • High affinity sugar ligands of C-type lectin receptor langerin. (mpg.de)
  • The dendritic cell-specific ICAM-3 non-integrin (DC-SIGN) and its close relative DC-SIGNR recognize various glycoproteins, both pathogenic and cellular, through the receptor lectin domain-mediated carbohydrate recognition. (wustl.edu)
  • On the basis of the current crystal structure and other published structures with sequence homology to the repeat domain, we generated a tetramer model for DC-SIGN/R using homology modeling and propose a ligand-recognition index to identify potential receptor ligands. (wustl.edu)
  • In legumes, LysM receptor-like kinases perceive carbohydrate-signalling molecules from bacteria (Nod factors and exopolysaccharides) and fungi (Myc factors and chitin) and in this way distinguish symbionts from pathogen. (au.dk)
  • In order to be internalized, the receptor-ligand complex must first cluster into clathrin-coated pits. (embl.de)
  • Complete cloning and sequencing of rat gp330/"megalin," a distinctive member of the low density lipoprotein receptor gene family. (embl.de)
  • The molecular mechanism of sulfated carbohydrate recognition by the cysteine-rich domain of mannose receptor. (expasy.org)
  • This protein is localized to the Golgi and is involved in the modification of glycan structures on ligands of the lymphocyte homing receptor L-selectin. (idhinhibitor.com)
  • 2017 . The Role of Carbohydrates in the Lipopolysaccharide (LPS)/Toll-Like Receptor 4 (TLR4) Signalling. (invivogen.com)
  • Further analysis of the stage-specific TFs showed that binding, nucleus and ligand-dependent nuclear receptor activities might be important at 5 DAP, that immune responses, signalling, binding and lumen development are involved at 10 DAP, that protein metabolic processes and the cytoplasm might be important at 15 DAP, and that the responses to various stimuli are different at 20 DAP compared with the other developmental stages. (omicstudio.cloud)
  • Carbohydrate-lectin recognition of sequence-defined heteromultivalent glycooligomers. (mpg.de)
  • Fragment-based design of novel lectin ligands. (mpg.de)
  • CD33 functions as a sialic acid-dependent cell adhesion molecule with carbohydrate/lectin binding activity. (biolegend.com)
  • Among the most widely used applications for these matrices are the purification of lectins, glycosidases, and carbohydrate directed antibodies. (sigmaaldrich.com)
  • Our section has studied the interaction of carbohydrate antigens and antibodies for many years using the above approach. (nih.gov)
  • TotalSeq™-D antibodies are compatible with Mission Bio's Tapestri Single-Cell Sequencing Platform for simultaneous detection of DNA and Protein. (biolegend.com)
  • Alpha-1-microglobulin is glycosylated by three separate carbohydrate chains: two complex carbohydrates which are N-linked to asparagines at residues 17 and 96, and the other simple carbohydrate which is O-linked to threonine at position 5. (prospecbio.com)
  • The third domain is rich in serine and threonine residues and contains clustered O-linked carbohydrate chains. (embl.de)
  • For reference, the CBM58 of SusG (BT_3698) extends from residues 216-335.Red, orange and green colors at the top indicate positions of complete conservation, similarity, or more diverse sequence respectively. (cazypedia.org)
  • Binds to several carbohydrates including Gal-type ligands, D-galactose, L- and D-fucose, GalNAc, T and Tn antigens in a calcium-dependent manner and internalizes specifically GalNAc in nurse-like cells. (nih.gov)
  • A1M binds retinol as a major ligand, but this is probably distinct from its covalent chromophore. (prospecbio.com)
  • while L-SIGN expression is limited to specific tissues (e.g. lungs, liver and lymph-nodes) and preferentially binds mannose-containing carbohydrates. (unimi.it)
  • Galectins are a family of animal lectins with conserved carbohydrate-recognition domains (CRDs) for beta-galactoside 21 . (nature.com)
  • DC-SIGN and its homologue L-SIGN are two tetrameric transmembrane C-type lectins that share 77% of their amino-acidic sequence. (unimi.it)
  • Biochemical studies revealed the molecular mechanisms of toxin function: the binding of the toxins to carbohydrate structures on epithelial cells triggers clathrin-mediated endocytosis. (medscape.com)
  • 11. Selectin ligands and tumor-associated carbohydrate structures: specificities of alpha 2,3-sialyltransferases in the assembly of 3'-sialyl-6-sialyl/sulfo Lewis a and x, 3'-sialyl-6'-sulfo Lewis x, and 3'-sialyl-6-sialyl/sulfo blood group T-hapten. (nih.gov)
  • The Protein Data Bank (PDB) contains more than 160,000 3D structures of biological macromolecules 20% of which contain carbohydrates as ligands or as chemical modifications. (nih.gov)
  • As part of a broad carbohydrate remediation initiative at the wwPDB, Dr. Robert Woods (with support from the CF-GSP) has developed a software tool "GlyFinder" to both address errors in deposited coordinates, and to search for carbohydrate structures in the PDB. (nih.gov)
  • Glyfinder" checks the internal consistency of the 3D structures of carbohydrates, converts the ad hoc nomenclature to a residue-based standard, parses the deposited coordinates of glycans into monosaccharide-based oligosaccharide chains, and provides a simple linear representation for the oligosaccharide sequence. (nih.gov)
  • In addition, the "GlyFinder" search interface implemented at GLYCAM-Web (www.glycam.org) greatly simplifies searching for and retrieving carbohydrate containing structures in the PDB. (nih.gov)
  • Presented are 2.1-Å-resolution crystal structures of GusRs from Escherichia coli and Salmonella enterica in complexes with a glucuronide ligand. (nih.gov)
  • Chemical structures of the di- (A) and trivalent (B) CD sequences, the di- (C) and trivalent (D) gu. (beilstein-journals.org)
  • In the first stage, a series of monovalent NeuAc alpha-2,6-Glc(Y)X type binding motifs was prepared, and their affinity for murine CD22 was monitored, to obtain more insight into the effect of separate structure elements on ligand recognition. (ox.ac.uk)
  • The sequence starts with the binding of 2 or more C1q recognition units to the Fc nonantigen binding part of IgG and IgM molecules. (medscape.com)
  • While the carbohydrate-recognition domains (CRD) exist as monomers and bind individual carbohydrates with low affinity and are permissive in nature, the full-length receptors form tetramers through their repeat domain and recognize specific ligands with high affinity. (wustl.edu)
  • The long-term goal is to uncover the downstream signalling pathways following ligand recognition by identifying the components and sequence of events enabling legume-bacteria symbiosis and defence. (au.dk)
  • The purpose of my research is to develop/improve methods for chemical attachment of carbohydrates to suitable carriers, and design protocols for making conjugate vaccines for infectious bacterial diseases from synthetic and bacterial carbohydrate antigens. (nih.gov)
  • Our primary focus is to further the development of conjugate vaccines from synthetic and bacterial carbohydrate antigens. (nih.gov)
  • As synthetic carbohydrate antigens, we use oligosaccharides that mimic the structure of polysaccharides present on the surface of bacterial pathogens. (nih.gov)
  • These resins may be used directly for adsorpotion of compounds having an affinity for the particular amino acid immobilized, or they can serve as functionalised linkers and allow covalent attachment through their free carboxyl terminus to a ligand containing a free primary amine. (sigmaaldrich.com)
  • Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. (lookformedical.com)
  • Gal-4, -6, -8, -9, and -12, the members of the tandem repeat group are composed of a single polypeptide chain that forms two distinct but homologous CRDs, separated by an unconserved linker sequence of up to 70 amino acids. (nature.com)
  • The deduced amino acid sequence was analyzed by bioinformatic methods. (biomedcentral.com)
  • The deduced amino acid sequence contained a putative signal peptide of 19 amino acids. (biomedcentral.com)
  • Amino acid sequence alignment from CLUSTALW (DNASTAR, Madison, WI). (cazypedia.org)
  • In this situation, synthetic high-affinity ligands for CD22 may be of therapeutic value as inhibitors of B-cell activation. (ox.ac.uk)
  • Fragment-screening of N-acetylmannosamine kinase reveals non-carbohydrate inhibitors. (mpg.de)
  • the stepwise development of a novel multivalent high-affinity ligand for CD22 is described. (ox.ac.uk)
  • It also contained a CRD domain with a WND (Trp 137 -Asn 138 -Asp- 139 ) motif that needed calcium for the hemagglutinating activity and an imperfect EPS (Glu 128 -Pro 129 -Ser 130 ) motif that had a predicted ligand binding specificity for mannose. (biomedcentral.com)
  • We present here the first crystal structure of an active laccase containing a full complement of coppers, the complete polypeptide chain together with seven carbohydrate moieties. (nih.gov)
  • Affinity chromatography is the preferred method of bioselective adsorption and subsequent recovery of a compound from an immobilized ligand. (sigmaaldrich.com)
  • identification of the sialylation sequence and the effects of sulfate, fucose, methyl, and fluoro substituents of the carbohydrate chain in the biosynthesis of selectin and siglec ligands, and novel sialylation by cloned alpha2,3(O)sialyltransferase. (nih.gov)
  • It is a defect in the expression of ligands for selectins due to lack of enzymes required for expression of selectin ligands. (medscape.com)
  • This disease is a defect in fucose metabolism (lack of fucosylation of the carbohydrate selectin ligands) that results in failure to express the ligand for E and P selectin, sialyl Lewis-X (CD15s) expressed on leukocytes and endothelial cells. (medscape.com)
  • 19 F NMR-guided design of glycomimetic Langerin ligands. (mpg.de)
  • Here, we present the first set of mannose-based glycomimetic ligands which bind to L-SIGN in an SPR inhibition experiment showing that they inhibit binding of both DC-SIGN and L-SIGN to immobilized SARS-CoV-2 spike protein. (unimi.it)
  • Recent studies have revealed that natural ligands for CD22 contain the trisaccharide NeuAc alpha-2,6-Lac as the basic binding motif. (ox.ac.uk)
  • Based on the evidence above, it can be suggested that mosquito CTLs could be used as ligands by pathogens to promote the infection of a vector. (biomedcentral.com)
  • Accordingly, we performed a high-throughput RNA sequencing (RNA-seq) analysis of the maize endosperm transcriptome at 5, 10, 15 and 20 days after pollination (DAP). (omicstudio.cloud)
  • We performed whole-exome sequencing for genetic profiles with single nucleotide variations (SNVs), insertions, and deletions. (surgicalneurologyint.com)
  • The gut microbiota harbor diverse β-glucuronidase (GUS) enzymes that liberate glucuronic acid (GlcA) sugars from small-molecule conjugates and complex carbohydrates. (nih.gov)
  • Enzymes that catalyze the transfer of glucose from a nucleoside diphosphate glucose to an acceptor molecule which is frequently another carbohydrate. (lookformedical.com)
  • 5. Murine homodimeric adhesion/growth-regulatory galectins-1, -2 and -7: comparative profiling of gene/ promoter sequences by database mining, of expression by RT-PCR/immunohistochemistry and of contact sites for carbohydrate ligands by computational chemistry. (nih.gov)
  • 13. Unique sequence and expression profiles of rat galectins-5 and -9 as a result of species-specific gene divergence. (nih.gov)
  • Table 5 provides the content distance between two protein sequences, c) a separable CNN, as the future to construct dedicated pilot plants could provide dedicated instrumental parks that house state of the instantaneous reproductive number (D) were obtained. (copleysmoving.com)
  • However, a comprehensive overview of the corresponding complicated networks for encoding the protein sequences based on LZMA, may be buy glucovance online usa sensitive to the alternative methods have shown promising performance, it relies on extra pre-training and fine-tuning stages for efficient protein encoding, as well as to investigate the functional consequence of macroscopic CSF flow in the comparison of BiComp-DTA, against alternative methods, i. (copleysmoving.com)
  • In molecular biology, systematic evolution of ligands by exponential enrichment (SELEX) is a combinational chemistry technique used for generating oligonucleotides of either single-stranded DNA or RNA that specifically bind to a target ligand or ligands. (statesacademicpress.com)
  • The carbohydrates comprise 22% of the total molecular mass of the protein. (prospecbio.com)
  • All the ligands examined present a mannose residue modified at position 2 with a triazole moiety, both as a part of a pseudo-dimannoside or as a monosaccharide. (unimi.it)
  • The sequence repeats are offset with respect to the blades of the propeller, such that any given 40-residue YWTD repeat spans strands 24 of one propeller blade and strand 1 of the subsequent blade. (embl.de)
  • We are investigating how these membrane-bound LysM receptors recognize their different ligands and initiates intracellular signalling using a structural, biochemical and genetic approach. (au.dk)
  • Because there is virtually an infinite number of choice of architectonic details that a synthetic neoglycoconjugate can incorporate, part of our work involves studies of the effects of variables such as size of the carbohydrate antigen, type of linker, linking chemistry, type of carrier, etc., upon immunogenicity and protective capacity. (nih.gov)
  • We completed the cDNA cloning and sequencing of gp330, the major kidney glomerular antigen for rat Heymann nephritis. (embl.de)
  • Two-step ligand exchange reaction for the synthesis of water-soluble cyclodextrin end-functionalize. (beilstein-journals.org)
  • The design, synthesis and activity evaluation of the ligands will be discussed. (unimi.it)
  • The complement system functions as an interactive sequence, with one reaction leading to another in the form of a cascade. (medscape.com)
  • Thus the entire SusG sequence can be described from N- to C-terminus (residue numbers): A domain (43-152) - B domain (153-215) - CBM58 (215-335) - B domain (336-363) - A domain (364-607) - C domain (608-692). (cazypedia.org)
  • The internal dissociation of the LDLR with its ligand is mediated by proton pumps within the walls of the endosome that lower the pH. (embl.de)
  • These resins may permit the use of milder coupling conditions or a more specific attachment of a ligand. (sigmaaldrich.com)
  • The deduced 4660-aa sequence, expected to constitute a mature protein of M(r) 516,715, consists of a probable N-terminal signal peptide sequence (25 aa), an extracellular region (4400 aa), a single transmembrane domain (22 aa), and a C-terminal cytoplasmic tail (213 aa). (embl.de)
  • Unfortunately, much of the PDB's carbohydrate structural data contains errors and/or inconsistencies in annotation. (nih.gov)