Ligand jurkat cells. Medical search. Frequent questions

Drugs that activate BAX, such as ABT-737, a BH3 mimetic, hold promise as anticancer treatments by inducing apoptosis in cancer cells. (wikipedia.org)
Apoptosis induction potential of each compound determined by mitochondrial membrane potential analysis (Rho123), cleaved caspase-3 expression analysis by flow cytometry and immunofluorescent staining and cell morphology analysis by giemza, hematoxylin and eosin and Papanicolaou protocols. (ijpsonline.com)
In the cell morphology analyzes, chromatin condensation and marginalization, changes in the cell membrane, ghost cells and apoptotic bodies were observed as evidence of apoptosis formation. (ijpsonline.com)
Pannexin-1 (Panx1) channels mediate the efflux of ATP and AMP from cancer cells in response to induction of extrinsic apoptosis by death receptors or intrinsic apoptosis by chemotherapeutic agents. (aspetjournals.org)
We previously described the accumulation of extracellular ATP /AMP during chemotherapy-induced apoptosis in Jurkat human leukemia cells. (aspetjournals.org)
Measured by its ability to inhibit Fas Ligand-induced apoptosis of Jurkat human acute T cell leukemia cells. (bio-techne.com)
Among TNF family members, DcR3 was shown to bind with Fas ligand (FasL) and LIGHT and inhibit FasL- and LIGHT-induced apoptosis. (bio-techne.com)
Thus, in addition to DcR1, DcR2 and OPG, DcR3 is another TNFR family molecule which modulates ligands that induce apoptosis. (bio-techne.com)
Another main TIM-3 ligand may be the membrane phospholipid phosphatidylserine (PS), open on the top of cells going through apoptosis and various other procedures [29,30], including T cell activation [31,32]. (biosemiotics2013.org)
The relationship between NKG2D and NKG2DLs sets off NK cell cytokine secretion and degranulation which induce apoptosis of focus on cells. (buyresearchchemicalss.net)
The colony-forming ability of the cells was evaluated using plate cloning assay, and cell cycle changes and cell apoptosis were analyzed with flow cytometry. (bvsalud.org)
It has been suggested in published literature that the beta-galactoside binding protein, galectin-9, acts on T cells to induce apoptosis, but whether this induced cell death is dependent on TIM3 remains an area of active debate and conflicting results. (emerging-researchers.org)
We hypothesized that Galectin-9 induced apoptosis of exhausted Jurkat T lymphocytes is dependent on T Cell Immunoglobulin and Mucin Protein 3 (TIM3). (emerging-researchers.org)
Galectin-9 protein ability to induce apoptosis was investigated using Jurkat T lymphocytes placed under an untreated negative control, a positive control of staurosporine, and 50, 100, or 200Nm of galectin-9 for 4, 12, and 18 hours. (emerging-researchers.org)
By using these methods to determine if galecin-9 induced apoptosis is dependent upon the T cell inhibitory receptor TIM3, researchers can develop TIM3 antibodies that can be used to block immune cell inhibition by cancer cells. (emerging-researchers.org)
I was responsible for treating and splitting all of the Jurkat cells used for the experiments, conducting caspase and tunnel assays to determine levels of apoptosis, determining the puromycin kill curve, performing CRISPR Cas9 gene knockout on Jurkat cells through insertion of puromycin resistance and CRISPR guide RNA. (emerging-researchers.org)
We have shown that Fn can induce apoptosis in immune cells (Jewett 2000), and it appears that aggregation of immune cells and upregulation of death ligands play a significant role (Huynh 2011, Fraser 2010). (umich.edu)
Peptidoglycan recognition proteins (PGRP-S) are responsible for detecting bacteria-derived peptidoglycans and for target recognition and induced death ligand mediated cell apoptosis (Sashchenko 2007). (umich.edu)
The purpose of this study was to determine if PGRP-S has a direct role in Fn induced apoptosis in Jurkat T cells. (umich.edu)
After co-culturing Fn with Jurkats overnight with monoclonal antibodies to PGRP-S, the levels of Jurkat apoptosis was assessed via caspase assay according to manufacturer instructions (Promega Caspase-Glo 3/7 Assay Kit). (umich.edu)
There was a significant dose dependent decrease in Jurkat-cell apoptosis upon co-culture with Fn in the presence of monoclonal anti-PGRP-S antibodies. (umich.edu)
Treatment of murine lung epithelial cells or Jurkat T cells with doses of Hg similar to those present at the injection site results in a death process that is morphologically and biochemically distinct from apoptosis. (temple.edu)
Extensive lymphocyte severe infections is a massive loss of lymphocytes, den- apoptosis has also occurred in humans and animals infect- dritic cells, gastrointestial epithelial cells, and other cell ed with several exotic agents, including Bacillus anthracis , types through apoptosis, or programmed cell death. (cdc.gov)
Prevention of lymphocyte apoptosis, same process by which these cell populations are regulat- through either genetic modification of the host or treatment ed during normal health ( 1 , 2 ). (cdc.gov)
acquired defects in the control of apoptosis may lead to a Apoptosis, or programmed cell death, is the method variety of disease states. (cdc.gov)
For example, excessive inhibition by which tissue remodeling takes place during normal of apoptosis is an underlying mechanism of cancer, while growth and development and the physiologic mechanism an inappropriate increase is seen in some neurodegenera- by which labile cell populations such as gastrointestinal tive diseases and other conditions. (cdc.gov)

We routinely test this antibody on the fixed and permeabilized Jurkat cells by flow cytometry with Cytofix/Cytoperm (Cat. (bdbiosciences.com)

Signal transduction pathways guided by cellular receptors commonly exhibit low-level constitutive signaling in a continuous, ligand-independent manner. (stanfordhealthcare.org)
Ligand-independent signaling by the precursors of mature antigen receptors regulates development of B and T lymphocytes. (stanfordhealthcare.org)
Our results highlight a new role for LFA-1 in the core architecture of the IS that can be exploited to study the spatiotemporal redistribution of surface receptors on SLBs, thereby extending the potential of E6.1 cells and their derivatives for fine-scale imaging studies. (ox.ac.uk)
TIM-3 doesn't have a definable intracellular ITIM (immunoreceptor tyrosine-based inhibitory theme) or ITSM (immunoreceptor tyrosine-based change theme), motifs that normally characterize co-inhibitory recruit and receptors SH2 IL17B antibody domain-containing phosphatases to lessen T cell signaling [25]. (biosemiotics2013.org)
To initially investigate a model system, the researchers generated Jurkat cells with chimeric receptors comprising an extracellular PD-1 domain, an intracellular human CD3ζ signaling chain, and a luciferase reporter gene. (acir.org)
Understanding the role of T cell inhibitory receptors, such as TIM3, and their associated ligands has become the forefront of immuno-oncology research. (emerging-researchers.org)
By understanding the mechanisms of cancer cell activation of T cell inhibitory receptors like TIM3, researchers can devise breakthrough immunotherapy treatments for cancer patients by preventing immune cell inhibition through the blockade of inhibitory receptors. (emerging-researchers.org)
This setting of ligand discrimination continues to be implicated in several natural processes, including the serial engagement of T cell receptors by low affinity ligands and enhancing G-protein receptor activation [54,55]. (bioinbrief.com)
after incubation with interferon-gamma or toll-like receptors (TLR) ligands. (memorial2014.com)
Janus Kinase 3 (Jak3) belongs to the Jak family of protein tyrosine kinases that couple to cytokine receptors and are activated by ligand binding to these receptors. (rndsystems.com)
Optimal T cell activation typically requires engagement of both the TCR and costimulatory receptors, such as CD28. (aai.org)
The KS model postulates that, when small receptors engage their ligands within areas of close (∼15 nm) contact in the T cell/APC interface, this facilitates phosphorylation by segregating the engaged receptor/ligand complex from receptor protein tyrosine phosphatases with large ectodomains, such as CD45. (aai.org)

DESIGN/METHODS/RESULTS: Our results show that Jurkat cells overexpressing CXCR1 or the receptor carrying single polymorphism CXCR1_300 or CXCR1_142 are able to adhere and migrate in response to both IL-8 and p17. (unibs.it)
Prior research show that Jurkat cells concomitantly express high levels of chemokine receptor CXCR4 [16] and integrin L2 [17] which recognize the chemokine CXCL12 and endothelial ICAM-1, respectively. (bioinbrief.com)

Galectin-9 is certainly considered to induce T cell loss of life by binding to sugars on TIM-3, although various other work provides refuted this [27,28]. (biosemiotics2013.org)
We show here that these lipids by themselves can induce calcium signaling in T cells, also when having protein ligands on the SLB. (ox.ac.uk)
Conclusion: The results indicate that PGRP-S has a direct role in Fusobacterium nucleatum 's ability to induce apoptotic cell death in Jurkat T cells. (umich.edu)

They used the inhibition of proprotein convertases ( PCSK1 ) in T cells and programmed death-ligand 1 ( CD274 ) in cancer cells. (emerginginvestigators.org)

In healthy mammalian cells, the majority of BAX is found in the cytosol, but upon initiation of apoptotic signaling, Bax undergoes a conformational shift. (wikipedia.org)
However, the applicability of such traditional chemotherapeutic approaches is primarily limited due to acquired Multi-Drug Resistance (MDR) in cells, which is acquired through the production of anti-apoptotic cascades and the overexpression of cell membrane-based drug efflux pumps, such as P-glycoprotein, for self-defense. (ijpsonline.com)
Robust extracellular ATP/AMP accumulation was observed in the FADD-deficient cells during necroptosis, but not during apoptotic activation of Panx1 channels. (aspetjournals.org)
Accumulation of extracellular ATP/AMP was similarly absent in RIP1-deficient Jurkat cells during apoptotic responses to chemotherapeutic agents. (aspetjournals.org)
Apoptotic activation triggered equivalent proteolytic gating of Panx1 channels in all three Jurkat cell lines. (aspetjournals.org)
Binding and Crystallographic research have got since verified that TIM-3 binds PS [34], ADL5747 and TIM-3 may also facilitate binding to and engulfment of apoptotic cells (efferocytosis) by macrophages like its family members TIM-1 and TIM-4 [34C36]. (biosemiotics2013.org)
We asked whether PS is certainly an integral regulatory ligand for TIM-3's co-receptor function, beyond its function to advertise the engulfment of apoptotic cells when TIM-3 is certainly portrayed on macrophages. (biosemiotics2013.org)
Other biological effects not leading to apoptotic cell death have also been described. (biolegend.com)
ED 50 = 10 - 50 ng/ml, corresponding to a specific activity of 0.02 - 0.1 x 10 6 units/mg, as determined by inhibition of apoptotic cell death induced by FASL on Jurkat cells. (biolegend.com)
These findings suggest that inter- the apoptotic destruction of lymphocytes and dendritic ventions aimed at reducing the extent of immune cell apop- tosis could improve outcomes for a variety of severe cells could have a particularly adverse effect on disease human infections, including those caused by emerging outcome. (cdc.gov)

We used a cell-permeable peptide (S3 peptide) that contains the phosphorylation site (Ser-3) of cofilin to inhibit the cellular function of LIMK1. (neuroprobe.com)
Since cancer cells inhibit T-cell activity, the authors investigated a method to reverse T-cell disfunction with gene therapy, so that the T-cells would become effective once again in fighting cancer cells. (emerginginvestigators.org)
e Relationship between TLR2 or TLR4 appearance and percent of B7-H1-positive cells (Pearsons relationship) Appearance.Furthermore, both ligands activate or inhibit T cells in AML, based on their degree of appearance, which may be modified in vitro and in vivo simply by chemotherapy [18]. (memorial2014.com)
Fig. 2: Anti-CD80 TKMG48 enables PD-1 to inhibit T cell activation in the presence of CD80. (nature.com)
Fig. 3: Anti-human CD80 TKMF5 enables human PD-1 to inhibit T cell activation in the presence of human CD80 by dissociating cis duplex of human PD-L1 and CD80. (nature.com)

FAS was initially purified and cloned from SKW6.4 cells and a cDNA library of human T cell lymphoma KT-3 cells. (biolegend.com)
LEADS TO vitro Characterization of T Lymphocyte Arrest To recognize feature dynamics of T lymphocyte arrest, we performed initial tests with Jurkat T cells, a lymphoma cell range found in research of T cell signaling and adhesion [3C5] widely. (bioinbrief.com)
These changes are thought to lead to tumor-microenvironment interactions via factors secreted by stromal or epithelial cells in response to lymphoma cell-produced cytokines ( 7 ). (spandidos-publications.com)
Because transduced normal B cells expressed only low levels of FasL and did not kill Fas-expressing Jurkat or A20 B lymphoma cells in vitro, these data suggest that gld B cells are not tolerogenic due to unique characteristics of these B cells rather than the lack of functional FasL expression. (usuhs.edu)
Angioimmunoblastic T-cell lymphoma (AITL) is one of the most common subtypes of peripheral T-cell lymphoma. (biomedcentral.com)
B cell lymphoma 6 (Bcl6) is considered to be the lineage defining transcription factor for T follicular helper cells (T FH ) [ 20 ]. (biomedcentral.com)

In the case of hematopoietic stem and progenitor cells, the dormancy of the most primitive HSPC is maintained by the bone marrow niche by means of several key molecular interactions between receptor-ligand pairs1C3. (conferencedequebec.org)

In this study, we compared how different signaling pathways determine extracellular nucleotide pools in control Jurkat cells versus Jurkat lines that lack the Fas-associated death domain (FADD) or receptor-interacting protein kinase 1 (RIP1) cell death regulatory proteins. (aspetjournals.org)
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. (bio-techne.com)
HIV-1 matrix protein p17, released from infected cells, was found to exert an interleukin (IL)-8 chemokine-like activity on human monocytes, promoting their trafficking and sustaining inflammatory processes, after binding to CXCR1. (unibs.it)
cells and T cells identifies the associates of two ligand households: MHC course I string related substances (MICA and MICB) and UL16 binding protein (ULBP1-6). (buyresearchchemicalss.net)
To disentangle the elusive lipid-protein interactions in T-cell activation, we investigate how externally imposed variations in mobility of key membrane proteins (T-cell receptor [TCR], kinase Lck, and phosphatase CD45) affect the local lipid order and protein colocalisation. (scilifelab.se)
Our data suggest that the cellular membrane is poised to modulate the frequency of protein encounters upon alterations of their mobility, for example in ligand binding, which offers new mechanistic insight into the involvement of lipid-mediated interactions in membrane-hosted signalling events. (scilifelab.se)
Genome-wide CRISPR-Cas9 screening reveals ubiquitous T cell cancer targeting via the monomorphic MHC class I-related protein MR1. (bmj.com)
Stromal cell-derived factor 1 α (SDF-1α), the ligand for G-protein-coupled receptor CXCR4, is a chemotactic factor for T lymphocytes. (neuroprobe.com)
To elucidate the distinctive molecular characteristics and functional implications of the CXCR4-LPA 1 heteromer, we performed various assays, including cAMP, BRET for G protein activation, β-arrestin recruitment, ligand binding, and transwell migration assays. (biomedcentral.com)
T cell immunoglobulin and mucin protein 3 (TIM3) is a cell surface protein expressed on T helper type 1 cells and is proposed to be a regulator of human T cell exhaustion. (emerging-researchers.org)
TIM3 interacts with cellular ligands by functioning as an inhibitory protein to limit normal T cell function, thus enhancing cellular exhaustion. (emerging-researchers.org)
Results Immunohistochemical analysis eldepryl va availability personnel svc of S100A7 locution in verbal leukoplakia lesions and cancers To determine the clinical significance of S100A7 protein in head-and-neck tumorigenesis, its saying was analyzed in clinical specimens from HNSCC, strong leukoplakia lesions with squamous cell hyperplasia or dysplasia, and histologically conventional tissues, using a specific monoclonal antibody alongside immunohistochemistry. (upb.ro)
LAMP-1 is a ~110 kDa type I transmembrane protein that is heavily glycosylated and widely expressed by cells primarily on the luminal surface of their lysosomes. (bdbiosciences.com)
Targeting METTL3 by single-guide RNA, nanoparticle small interfering RNA (siRNA), or pharmacological inhibitor (STM2457) in combination with anti-programmed cell death protein 1 (PD-1) synergized to reinvigorate cytotoxic CD8+ T cells and mediate tumor regression. (bvsalud.org)
After induction with lipopolysaccharide, the expression of tsGILT mRNA was upregulated in spleen and kidney and recombinant tsGILT protein transferred to late endosomes and lysosomes in HeLa cells. (bvsalud.org)
In susceptible mice, Hg treatment induces both polyclonal activation of B cells, as well as the production of highly specific autoantibodies targeting the nucleolar protein fibrillarin. (temple.edu)
We have observed peripheral movement of the nucleolar protein fibrillarin via confocal microscopic study of Hg-induced cell death. (temple.edu)
These include members of the Bcl-2 protein family, course, and extent of programmed cell death will aid man- which have both proapoptotic and antiapoptotic activity agement of patients with severe infections. (cdc.gov)

These NKG2D ligands (NKG2DLs) are self-proteins having limited expression on regular tissues. (buyresearchchemicalss.net)
Furthermore to mRNA rules, the proteins degree of NKG2DLs on cell surface area can be controlled by dropping mediated through metalloproteinases (17C19). (buyresearchchemicalss.net)
For instance, the membrane-bound ULBP2 could be proteolytically released from malignancy cell lines such as for example HCT116 (cancer of the colon), HL60 and Jurkat (leukemia) cells which the proteins shedding is definitely reducible by matrix metalloprotease (MMP) inhibitors (19). (buyresearchchemicalss.net)
Aggregation and mobility of membrane proteins interplay with local lipid order in the plasma membrane of T cells. (scilifelab.se)
Using spectral imaging with polarity-sensitive membrane probes in model membranes and live Jurkat T cells, we find that partial immobilisation of proteins (including TCR) by aggregation or ligand binding changes their preference towards a more ordered lipid environment, which can recruit Lck. (scilifelab.se)
Ligand binding induces conformational changes in GPCRs followed by intracellular signaling through activation of heterotrimeric G proteins and recruitment of β-arrestins. (biomedcentral.com)
In this paper, the authors use MD simulations to construct and simulate a model of the periplasmic space, the peptidoglycan layer and its associated proteins, in an Escherichia coli cell. (emerginginvestigators.org)
These analyses also showed that constraining proteins to two sizes in the cell surface greatly enhances random relationships those between the membrane and the cytoplasm. (tech-strategy.org)
Simulations of TCR-pMHC complex formation based on these findings suggest how unclustered TCR triggering-associated proteins might nevertheless be capable of generating complex signaling outputs via the differential recruitment of cytosolic effectors to the cell membrane. (tech-strategy.org)
Very recently however high resolution measurements of the behavior of proteins in the cell/glass interface suggested the TCR is definitely instead preclustered in groups of 7-25 molecules in resting cells (5). (tech-strategy.org)
Tim proteins are a family of immune cell surface molecules that have been implicated in modulation of autoimmune diseases in both humans and animals. (temple.edu)
LPS-activated B cells, transduced with IgG fusion proteins, are highly tolerogenic APCs. (usuhs.edu)
When B cells from B7 knockout donors were transduced to express Ig fusion proteins, they were not tolerogenic in two different mouse strains and Ag models. (usuhs.edu)

Leukemia cells in patients are intrinsically resistant to standard chemotherapy, and resistance development in leukemia cells toward drugs results in treatment failure[ 4 , 5 ]. (ijpsonline.com)
The inducibility of B7-H1 appearance by TLR or IFN- ligands shows that several stimuli, either produced through the immune system response against leukemia cells or released by infectious microorganisms, could secure leukemic cells from T cells. (memorial2014.com)
Mouse J774 macrophage cells and human Jurkat T-cell leukemia cells. (alomone.com)

Inhibition of cytotoxic effect of FASL on Jurkat cells in the presence of recombinant human FAS. (biolegend.com)
The interaction of FAS/FASL in immature dendritic cells (DCs) induces functional maturation. (biolegend.com)
In addition, FAS/FASL interaction increases the production of chemokines in NK-T cells, and FASL acts as a survival factor for human CD34 + cells and increases their colony formation. (biolegend.com)
tolerogenic, initially suggesting a role for Fas ligand (FasL) in tolerance. (usuhs.edu)

Methods The MR1-restricted T cell clone, MC.7.G5 (7G5.TCRT), with reported cancer specificity and pan-cancer activity, 1 was cloned and expressed via lentiviral transduction in Jurkat E6.1 TCRαβ- β2M- CD8+ NF-κB:CFP NFAT:eGFP AP-1:mCherry cells or in human donor T cells (with or without CD8 or after knockout of the TRBC1 and 2 genes with CRISPR Cas9). (bmj.com)
Conversely, CXCL12-induced calcium signaling and migration were increased in LPAR1 knockout cells, and LPA 1 -selective antagonists enhanced CXCL12-induced Gα i/o signaling and cell migration in the parental MDA-MB-231 cells but not in LPA 1 -deficient cells. (biomedcentral.com)
CRISPR-Cas9 knockout was used to inactivate TIM3 in Jurkat T lymphocytes and in vitro CRISPR-Cas9 digest was performed to determine the efficiency of each CRISPR guide RNA used during the CRISPR-Cas9 knockout. (emerging-researchers.org)
Upon TLR ligand challenge, IRAK4 knockout mice exhibit severely impaired NF-kappa B activation. (rndsystems.com)

Very latest studies, however, have got revealed the fact that inhibitory ramifications of TIM-3 on anti-tumor immunity in fact originate in dendritic cells, rather than T cells [18]. (biosemiotics2013.org)
Analysis of the tumor-infiltrating myeloid populations at days 3, 7, and 14 post tumor inoculation revealed that the majority of myeloid cells were macrophages, which far outnumbered cross-presenting dendritic cells (DCs). (acir.org)

Mitochondrial membrane potential was found at low levels in cells treated with Schiff base compounds and doxorubicin. (ijpsonline.com)
TTYH family members form tetrameric complexes at the cell membrane. (cuni.cz)
Supported lipid bilayers (SLBs) are one of the most common cell-membrane model systems to study cell-cell interactions. (ox.ac.uk)
Gene transcription, microRNA rules, and proteolytic cleavage from your cell membrane are some systems that may regulate NKG2DL manifestation on tumor cells (1). (buyresearchchemicalss.net)
Sawada R, Lowe JB, Fukuda M. E-selectin-dependent adhesion efficiency of colonic carcinoma cells is increased by genetic manipulation of their cell surface lysosomal membrane glycoprotein-1 expression levels. (bdbiosciences.com)
Using two biophysical methods that mitigate these effects bioluminescence resonance energy transfer and two-color coincidence detection microscopy we display that within the uncertainty of the methods the membrane components of the TCR triggering apparatus the TCR complex MHC molecules CD4/Lck and CD45 are specifically monovalent or monomeric in human being T cell lines implying that TCR triggering depends only within the kinetics of TCR/pMHC relationships. (tech-strategy.org)
The effect is therefore to impair repolarization of the neuronal cell membrane, leading to hyperexcitability of the central nervous system (CNS). (medscape.com)

Inhibition of PRL-3 can stop the migration and invasion of metastatic cancers cells both in vitro and in vivo (27, 28). (buyresearchchemicalss.net)
Consistent with in vitro observations, in mice with PD-L1-deficient MC38 colorectal tumors where only host cells were the source of PD-L1, antibody blockade of both PD-1/PD-L1 and PD-L1/B7-1 interactions reduced tumor growth, while blocking only PD-L1/B7-1 interactions slightly accelerated tumor growth due to an increased availability of PD-L1. (acir.org)
Interest in events occurring at the site of Hg injection has prompted us to perform in vitro studies of Hg-induced cell death. (temple.edu)
By day 7 after injection, ∼95% of the transduced cells had divided at least once, presumably an effect of the in vitro LPS activation into the cycle, because resting cells did not divide. (usuhs.edu)

Transcription of MICA and MICB offers been shown to become inducible by warmth shock, oxidative tension or cell proliferation (8C10), whereas ULBP1-3 manifestation could be suppressed by histone deacetylase 3 (HDAC3) in epithelial tumor cells (11). (buyresearchchemicalss.net)

In this study, anticancer properties of heterocyclic salicylaldimines and their Cu(II) complexes on K562 human chronic myelogenous leukemia cell line were investigated. (ijpsonline.com)
Compounds (C1-8 and doxorubicin) were placed to 96-well culture plate in triplicate order at doses of 1, 10, 100, 1000 μM and then K562 cells were seeded into the wells at the dose of 105/ml. (ijpsonline.com)
K562 cells expressing PD-L1, B7-1, or both were used as artificial APCs. (acir.org)
The lack of NK cell-mediated cytotoxicity was confirmed with K562 cells as goals. (memorial2014.com)
We viewed B7-H1 appearance in a number of myeloid lines (U937, K562, KG1a, HL60, THP-1) and lymphoid lines (Raji, Jurkat). (memorial2014.com)
a Stream cytometry evaluation of B7-H1 appearance in myeloid leukemic cell lines (LAMA-84, HL60, K562, U937, KG1a, THP-1) and lymphoid lines (Raji, Jurkat) with or without incubation with 500?IU/ml IFN- for 24?h. (memorial2014.com)

Differential expression of particular ectonucleotidases in tumor cell variants will determine whether chemotherapy-induced activation of Panx1 channels drives accumulation of immunostimulatory ATP versus immunosuppressive adenosine within the tumor microenvironment. (aspetjournals.org)
The enhancement in early signaling E6.1 LFA-1high cells did not affect expression of the early activation marker CD69 but led to an increase in IL-2 expression. (ox.ac.uk)
Hence, it is possible that the nickel-chelating lipids act as ligands to the cell's TCRs, an interaction that needs to be blocked to avoid unwanted cell activation. (ox.ac.uk)
Furthermore, activation from the ataxia telangiectasia mutated (ATM)/ ATM-and Rad3-related (ATR) kinase-mediated DNA harm response has been proven to upregulate the manifestation of NKG2DLs in a variety of tumor cell types (12, 13). (buyresearchchemicalss.net)
SDF-1α significantly induced the activation of LIMK1 in Jurkat human leukemic T cells and peripheral blood lymphocytes. (neuroprobe.com)
CD6 attenuates early and late signaling events, setting thresholds for T-cell activation. (ox.ac.uk)
The T lineage glycoprotein CD6 is generally considered to be a costimulator of T-cell activation. (ox.ac.uk)
Calcium responses, and also late indicators of T-cell activation such as IL-2 release, were also diminished in TCR-activated Jurkat cells expressing human CD6, compared with CD6-deficient cells or cells expressing a cytoplasmic deletion mutant of human CD6. (ox.ac.uk)
Finally there has been speculation that MHC class II forms practical dimers of dimers centered principally within the 1st crystal structure of HLA-DR (11 12 However other evidence points to there becoming no higher level of business above the MHC heterodimer (discussed in Ref. 13) and a role for its oligomerization in T cell activation is definitely unproven (12). (tech-strategy.org)
In addition to TCR binding to cognate peptide-MHC (pMHC), the engagement of a second "costimulatory" receptor is usually required for the full activation and differentiation of naive T cells. (aai.org)

For instance, binding of HA-BAD to BCL-xL and concomitant disruption of BAX:BCL-xL interaction was found to partly reverse paclitaxel resistance in human ovarian cancer cells. (wikipedia.org)
Human pluripotent stem cell-derived hepatocytes (hPSC-Heps) may be suitable for treating liver diseases, but differentiation protocols often fail to yield adult-like cells. (bvsalud.org)
Furthermore, these mice display increased expression of ASD-associated RORα target genes in both the brains of the BTBR mice and in a human neuroblastoma cell line treated with SR1078. (epiphanyasd.com)
A similar PD-L1 expression distribution was observed in RNASeq data from human non-small cell lung cancer (NSCLC) tumors. (acir.org)
We observed that CXCR4 forms heteromers with LPA 1 in recombinant HEK293A cells and the human breast cancer cell line MDA-MB-231. (biomedcentral.com)
Human FAS, amino acids (Arg17-Asn173) (Accession# NM_000043), was expressed in 293E cells. (biolegend.com)
Measuring calcium mobilization in single cells responding to superantigen, we found that human T cells expressing rat CD6 react significantly less well compared with T cells not expressing the exogenous receptor. (ox.ac.uk)
These authors find that genetic editing of both RPS6KB1 and PPARGC1A revitalizes a human skin fibroblast cell line. (emerginginvestigators.org)
nevertheless, its appearance in human cancers cell lines provides were modest. (memorial2014.com)

Strategies and Components Cell lifestyle The NF-B/Jurkat/GFPTM Transcriptional Reporter cell range was extracted from Program Biosciences, and Raji B cells had been extracted from the American Type Lifestyle Collection. (biosemiotics2013.org)
NF-B reporter Jurkat cells and Raji cells had been cultured in RPMI-1640 mass media supplemented with 10% FBS, 100?U/ml penicillin, and 100?g/ml streptomycin. (biosemiotics2013.org)
NF-B reporter Jurkat, Raji, HEK293 LTV, and PBMCs had been cultured at 37C with 5% CO2 within a humidified environment. (biosemiotics2013.org)

Era of cytotoxic T cells T cells in the peripheral bloodstream of a wholesome donor had been isolated utilizing a Skillet T Cell Isolation Package (Miltenyi Biotec) and cultured in RPMI 1640 (Lifestyle Technology) supplemented with 10% fetal leg serum, 100?IU/ml penicillin, 100?mg/ml streptomycin, 2?mM l-glutamine, 50?M -mercaptoethanol and 20?IU/ml interleukin 2 (PeproTech, Rocky Hill, USA). (memorial2014.com)

Blocking the SLB with bovine serum albumin reduced the signaling fraction to 11%, while preserving CD2 binding and the exclusion of the phosphatase CD45 from the cell-SLB contacts. (ox.ac.uk)
CD45 has no apparent ligand but there WK23 has been much desire for the WK23 possibility that it too is definitely controlled by oligomerization. (tech-strategy.org)
Confocal microscopy revealed that elongated CD80 molecules were less well segregated from CD45 at the T cell/APC interface. (aai.org)

This compound showed a half-maximal inhibitory concentration value of 300 pM (4.3?ng/mL) in a migration assay using Jurkat cells36. (conferencedequebec.org)

Many malignant cells release the NKG2D ligand ULBP2 off their cell surface area to evade immunosurveillance by organic killer cells and CD8 T cells. (buyresearchchemicalss.net)
In addition to mobilizing HSPC, the interference with the CXCR4-SDF1 axis has also been proposed as a possible strategy to mobilize malignant stem cells from their protective niche, hence making tumor stem cells even more susceptible to irradiation or chemo- therapy. (conferencedequebec.org)
CD20 is a B-cell-specific antigen expressed on malignant FL cells. (spandidos-publications.com)

It is also expressed on the surface of activated platelets, activated lymphocytes, cytotoxic T cells and NK cells, and some tumor cell lines, including U937 and KG1a. (bdbiosciences.com)

Despite these variants, the topography of different ligands with NKG2D, and in addition their efficiency of triggering NK cytolysis are equivalent (5, 6). (buyresearchchemicalss.net)
1 2 The diversity of MR1-ligand biology has broadened with the observation that single nucleotide variants (SNVs) exist within MR1 and that allelic variants can impact host immunity. (bmj.com)
Conclusions These data demonstrate that healthy individuals can harbor T cells reactive to MR1 variants displaying self-ligands expressed in cancer and benign tissues. (bmj.com)
With this scenario, a solid chemokine agonist can be expected to stimulate solid T cell arrest of multiple cell populations despite wide variants in surface area receptor expression. (bioinbrief.com)

By comparison, Jurkat cells have been used only scantily for imaging on supported lipid bilayers (SLBs) incorporating laterally mobile TCR and integrin ligands, which allow to study synaptic rearrangements of surface molecules and the fine architecture of the mature IS, likely due to limitations in the assembly of immune synapses with well-defined architecture. (ox.ac.uk)
GPCRs have been extensively studied as drug targets because they are involved in essential physiological processes and pathological conditions and have drug-accessible sites at the cell surface [ 3 ]. (biomedcentral.com)
The concentration of surface ligands were 102 sLex/m2, 103 CC/m2, and 103 ICAM-1/m2. (bioinbrief.com)
Another key molecular axis is the interaction between MK2-IN-1 hydrochloride stromal cell-derived factor 1 (SDF1 or CXCL12) and its receptor CXCR4, expressed on the cell surface of HSPC. (conferencedequebec.org)
approach that examined TCRs diffusing in the apical surface of T cells resting on a glass surface which strongly suggested the TCR is definitely monovalent (4). (tech-strategy.org)
As cells die, there is extrusion of fibrillarin from the nucleus and exposure at the cell surface. (temple.edu)

To test this hypothesis, we examined the effect of elongating the extracellular region of the CD28 ligand, CD80, on its ability to costimulate IL-2 production by primary T cells. (aai.org)

Here we have explored whether upregulating the low levels of endogenous LFA-1 expression on Jurkat E6.1 cells through transduction with CD11a- and CD18-encoding lentiviruses can improve IS architecture. (ox.ac.uk)

More recently, these cells have been exploited in imaging studies to identify key players in immunological synapse (IS) assembly in superantigen-specific conjugates and to track the dynamics of signaling molecules on glass surfaces coated with activating anti-CD3 antibodies. (ox.ac.uk)
Certainly, preventing TIM-3 engagement in mice with antibodies or soluble TIM-3 extracellular area was found to improve TH1 cell proliferation, and TIM-3 lacking mice showed flaws in immune system tolerance. (biosemiotics2013.org)
Significantly, however, the function performed by PS binding in modulating TIM-3 function in T cells is not elucidated though it was lately reported the fact that epitopes destined by immunomodulatory TIM-3 antibodies all overlap using the PS-binding site on TIM-3 [37]. (biosemiotics2013.org)
As experts in innate immunity and cell culture, we offer a large selection of products including PRR ligands , cell lines , selective antibiotics , antimicrobial agents , vaccine adjuvants , and antibodies . (invivogen.com)
Such cell death may allow recognition of fibrillarin by the immune system thereby promoting the formation of anti-nucleolar antibodies. (temple.edu)

T cell receptor-independent basal signaling via Erk and Abl kinases suppresses RAG gene expression. (stanfordhealthcare.org)
Here we describe a basal signal that controls gene expression profiles in the Jurkat T cell line and mouse thymocytes. (stanfordhealthcare.org)
To analyze the mechanisms for this B cell-delivered gene therapy, we first followed the fate of CFSE-labeled B cell blasts. (usuhs.edu)

2023 Jurkat-Lucia™ NFAT Cells for ADCC & ADCP Assays Hederman A.P. et al. (invivogen.com)

Introduction Functions of somatic stem cells are strictly governed by an appropriate balance between self-renewal and differentiation. (conferencedequebec.org)
Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. (bdbiosciences.com)
Transcriptome analysis revealed that Th1/Th2 differentiation, NK cells, Th17 cell, complement system and calcium signaling pathways were down-regulated post-treatment of Pam3CSK4. (bvsalud.org)
Precisely how mutant TET2 contributes to AITL oncogenesis is unclear, but experimental data has shown that hematopoietic stem cells (HSCs) are particularly vulnerable to disruption of TET2 function resulting in increased self-renewal capacity and altered terminal differentiation [ 18 ]. (biomedcentral.com)

T cells expressing CD28 harboring a key tyrosine-170 mutation were less sensitive to CD80 elongation. (aai.org)
These results demonstrate the importance of ligand dimensions for optimal costimulation of IL-2 production by T cells and suggest that the KS mechanism contributes to CD28 signaling. (aai.org)

A specific PLEKHA4 siRNA was transfected in glioma U251 and T98G cells, and the changes in cell proliferation ability were assessed by real-time cell analysis technology and Edu experiment. (bvsalud.org)
Likewise, lysophosphatidic acid receptor 1 (LPA 1 ) is implicated in cancer cell proliferation and migration. (biomedcentral.com)
Based on this, they used small interfering RNA to decrease the expression of KCNMB2 in A549 lung cancer cells, finding decreased cell proliferation and increased lung cancer cell death. (emerginginvestigators.org)

Jurkat T cells transfected with the non-signaling molecule rat CD48 were found to bind to ligand-free SLBs containing ≥2 wt% nickel-chelating lipids upon which calcium signaling was induced. (ox.ac.uk)
Targeted blockade of the checkpoint molecule programmed cell death 1 (PD-1) can activate tumor-specific T cells to destroy tumors, whereas targeted potentiation of PD-1 is expected to suppress autoreactive T cells and alleviate autoimmune diseases. (nature.com)
Tim-3, originally reported as a Th1 specific molecule, is expressed on T cells of various subsets, as well as antigen presenting cells and granulocytes. (temple.edu)

CXC chemokine receptor 4 (CXCR4) and its ligand CXCL12, both of which are overexpressed in many cancers, play a pivotal role in metastasis. (biomedcentral.com)
LPA or alkyl-OMPT inhibited CXCL12-induced migration in various cancer cells that endogenously express both CXCR4 and LPA 1 . (biomedcentral.com)
Ultimately, complete inhibition of cell migration toward CXCL12 and alkyl-OMPT was only achieved in the presence of both CXCR4 and LPA 1 antagonists. (biomedcentral.com)
The presence and impact of CXCR4-LPA 1 heteromers on CXCL12-induced signaling and cell migration have been evidenced across various cell lines. (biomedcentral.com)
Several research indicated that seductive get in touch with between CXCR4 portrayed on tumor cells and SDF1 in the specific niche market might represent an integral system for metastatic spread and tumor level of resistance37,38. (conferencedequebec.org)
Debate and Outcomes Effect on HSPC-niche connections mediated via SDF1-CXCR4 axis Amount?2A displays the adhesion behavior of HSPC towards the surrogate specific niche market model displaying SDF1 seeing that the ligand. (conferencedequebec.org)

The Jurkat E6.1 clone has been extensively used as a powerful tool for the genetic and biochemical dissection of the TCR signaling pathway. (ox.ac.uk)
We show that, while forced LFA-1 expression did not affect TCR recruitment to the IS, E6.1 LFA-1high cells assembled better structured synapses, with a tighter distribution of signaling-competent TCRs at the center of the IS. (ox.ac.uk)
studies resulted in the suggestion of the co-inhibitory signaling function for TIM-3 in T cells [16,17]. (biosemiotics2013.org)
Actually, most released research in T cells reveal a co-stimulatory than inhibitory function for TIM-3 in TCR signaling [6 rather, 19C22] although experimental support for co-inhibitory signaling continues to be reported [23 also,24]. (biosemiotics2013.org)
Right here, we explored the need for PS in regulating the consequences of TIM-3 on TCR signaling, utilizing a Jurkat cell model. (biosemiotics2013.org)
We discovered that the co-stimulatory aftereffect of TIM-3 on TCR signaling in Jurkat cells requires the TIM-3 extracellular area, suggesting ligand-dependent legislation. (biosemiotics2013.org)
Calcium Signaling in T Cells Is Induced by Binding to Nickel-Chelating Lipids in Supported Lipid Bilayers. (ox.ac.uk)
This is important to avoid "false" signaling events in cell studies with SLBs, but also to better understand the molecular mechanisms involved in T-cell signaling. (ox.ac.uk)
Both the signaling fraction and signaling time did not change significantly compared to ligand-free SLBs when adding the CD48-ligand rat CD2 to the SLB. (ox.ac.uk)
In addition, more cells signaled on ligand-free SLBs with copper-chelating lipids instead of nickel-chelating lipids and the signaling was found to be predominantly via T-cell receptor (TCR) triggering. (ox.ac.uk)
Our data suggest that CD6 is a signaling attenuator whose expression alone, i.e. in the absence of ligand engagement, is sufficient to restrain signaling in T cells. (ox.ac.uk)
of TCRs is definitely significantly reduced at Jurkat T cell/glass interfaces inside a signaling-sensitive manner. (tech-strategy.org)
The mechanisms of action by which RTX kills FL cells include complement-dependent cytotoxicity (CDC) ( 3 ), antibody-dependent cellular cytotoxicity (ADCC) ( 4 , 5 )-herein referred to as 'effector function' mechanisms-as well as CD20 signaling effects ( 6 ). (spandidos-publications.com)

Appearance also increased in Jurkat and U937 cells on 24-h incubation in 500?IU/ml IFN-, suggesting that leukemic cells could exhibit B7-H1 below appropriate conditions. (memorial2014.com)
Open up in another window Fig.?1 TLR and B7-H1 expression in leukemic cell lines and blast cells from AML. (memorial2014.com)

The metal complexes of the compounds had stronger cytotoxic effects and cleaved caspase-3 expression than their ligands. (ijpsonline.com)
The differences in extracellular ATP/AMP accumulation correlated with cell-line-specific expression of ectonucleotidases that metabolized the released ATP/AMP. (aspetjournals.org)
In contrast, the RIP1-deficient cells were defined by increased expression of tartrate-sensitive prostatic acid phosphatase as a broadly acting ectonucleotidase. (aspetjournals.org)
Using DNA microarrays and Northern blots to analyze unstimulated cells, we demonstrate that expression of a cluster of genes, including RAG-1 and RAG-2, is repressed by constitutive signals requiring the adapter molecules LAT and SLP-76. (stanfordhealthcare.org)
Many studies have suggested the fact that expression degree of NKG2DLs on tumor cells straight correlates with tumor susceptibility to NK cytolysis (2) and with cancers patient survival price (3). (buyresearchchemicalss.net)
OBJECTIVE@#To investigate the effect of lactic acid-induced upregulation of PLEKHA4 expression on biological behaviors of glioma cells and the possible molecular mechanism. (bvsalud.org)
The mRNA expression of PLEKHA4 was detected by PCR in glioma samples and controls and in glioma cells treated with lactic acid and glucose. (bvsalud.org)
Together, these experiments showed that PD-L1 expression on antigen-presenting cells (APCs) played a role in antitumor T cell responses via PD-1 interactions. (acir.org)
Interestingly, however, mature DCs in NSCLC tumors had the highest level of PD-L1 (and B7-1) expression on a per cell basis among intratumoral myeloid populations. (acir.org)
In the presence of chronic disease and chronic infection, normally functioning T cells become "exhausted", a state characterized by the expression of inhibitory receptor molecules. (emerging-researchers.org)
They observed the recovery of IL-2 expression in Jurkat cells, with increased recovery noted in a co-culture sample. (emerginginvestigators.org)
Using HeLa cells, they find MAT20 up-regulates expression of inflammation and cell cytotoxicity markers. (emerginginvestigators.org)

Our findings provide confidence that the stiffness of tetra-PEG hydrogels can be modulated over a physiological range without significantly impacting the transport rates of solutes to and from encapsulated cells. (bvsalud.org)
Here, we demonstrate that CD6 significantly reduces early and late T-cell responses upon superantigen stimulation or TCR triggering by Abs. (ox.ac.uk)
Here, seeking to better understand the genetic associations underlying non-small cell lung cancer, the authors screened hundreds of genes, identifying that KCNMB2 upregulation was significantly correlated with poor prognoses in lung cancer patients. (emerginginvestigators.org)

Leukemia is a blood cancer that develops in the hematopoietic system and presents in the bone marrow with or without peripheral blood cell involvement[ 1 , 2 ]. (ijpsonline.com)
Individual peripheral bloodstream mononuclear cells (PBMCs) had been a generous present through the lab of ADL5747 Susan Kaech (Yale College or university). (biosemiotics2013.org)
In recent years, peripheral HSPC have largely replaced bone marrow-derived cells for autologous transplants, and they have become the major source of stem cells also for allogeneic transplantations16C21. (conferencedequebec.org)

Across the concentration range of 50, 100, and 200 nM, and time range of 4, 12, and 18 hours, commercial galectin-9 does not increase the caspase activity relative to untreated cells. (emerging-researchers.org)

Blocking both interactions increased the number of tumor-infiltrating CD8 + T cells, including CD8 + T cells specific for the tumor neoantigen M86. (acir.org)
In contrast, blocking only the PD-L1/B7-1 interactions led to a smaller increase in CD8 + T cells, and no increase in M86-specific CD8 + T cells. (acir.org)
While resting low-affinity L2/ICAM-1 interactions support transient cell tethering under flow, inducible Rabbit polyclonal to Dicer1 high-affinity L2/ICAM-1 complexes mediate stable cell arrest [8]. (bioinbrief.com)
This balance is in turn regulated by interactions between MK2-IN-1 hydrochloride stem cells and their microenvironment-the so-called niche. (conferencedequebec.org)

The present study demonstrated that CA125 could bind to RTX and reduce its tumor cell killing activity. (spandidos-publications.com)

Chen, L. & Flies, D. B. Molecular mechanisms of T cell co-stimulation and co-inhibition. (nature.com)

53 , 54 Thus, we believe that focused optimization of RORα ligands will provide compounds that will have improved efficacy in this model. (epiphanyasd.com)

Numerous metalloproteinase inhibitors have already been identified to stop the discharge of NKG2DLs from tumor cells (18, 19). (buyresearchchemicalss.net)
MEK inhibitors, including AZD6244 and UO126, reduced B7-H1 appearance and restored CTL-mediated lysis of blast cells. (memorial2014.com)

Functional activity of 7G5.TCRT was demonstrated by cytotoxicity utilizing the xCELLigence analyzer and by ELISA for cytokine release after co-culture with cancer cell lines with or without loading of known MR1 ligands. (bmj.com)

Treatment of Jurkat cells with S3 peptide inhibited the SDF-1α-induced cofilin phosphorylation, actin reorganization, and chemotactic response of Jurkat cells. (neuroprobe.com)

One significant obstacle to TLS formation is reduced immune cell infiltration into the tumor microenvironment (TME). (emerginginvestigators.org)
Recent studies have shown that vasculature normalizing (VN) agents may override this defect to improve tissue perfusion and increased immune cell entry into the TME. (emerginginvestigators.org)
However, their effects on immune cell and tumor cell phenotype remain understudied. (emerginginvestigators.org)

Analysis of T cell responses at day 10 post inoculation revealed increased frequency and number of CD8 + T cells within PD-L1-deficient MC38 tumors of PD-L1 KO and PD-L1 ΔDC mice compared to WT controls. (acir.org)
Fig. 4: Anti-CD80 TKMG48 augments PD-1-binding capacities of DCs and suppresses antigen-specific T cell responses in vivo. (nature.com)
Tim-3 and its known ligand galectin-9 play a role in the down-regulation of immune responses, including experimental autoimmune encephalomyelitis and autoimmune conjunctivitis. (temple.edu)

Growing interest in exploring mechanically mediated biological phenomena has resulted in cell culture substrates and 3D matrices with variable stiffnesses becoming standard tools in biology labs. (bvsalud.org)

Here the authors investigate whether treating tumor cells with VN would reduce their immunosuppressive phenotype and promote production of chemokine that recruit immune cells and foster TLS formation. (emerginginvestigators.org)
For either motif, the net effect of multiple chemokines on T cell arrest is identical to an equivalent concentration of one chemokine. (bioinbrief.com)

Anatomy19

Organisms5

Diseases4

Chemicals and Drugs101

Analytical, Diagnostic and Therapeutic Techniques and Equipment15

Phenomena and Processes38

Information Science3

Apoptosis26

Flow cytometry1

Receptors12

Show that Jurkat cells2

Induce3

Death-ligand1

Apoptotic10

Inhibit5

Lymphoma6

Receptor-ligand1

Protein18

Proteins13

Leukemia cells3

FasL4

Knockout4

Dendritic cells2

Membrane7

Vitro4

Epithelial1

K5626

Activation10

Human9

Raji3

Cultured in RPMI-16401

CD453

Assay1

Malignant3

Lymphocytes1

Variants4

Surface6

Extracellular region1

Transduction1

Antibodies5

Gene3

Assays1

Differentiation4

CD282

Proliferation3

Molecule3

CXCR46

Signaling13

Leukemic2

Expression12

Significantly3

Peripheral3

Caspase1

Interactions4

Bind1

Molecular1

Efficacy1

Inhibitors2

Cytokine1

Phosphorylation1

Immune cell3

Responses3

Biology1

Chemokine2