• sequences
  • Management of genetic sequences: The purpose of this group of features is to help users identify, within large collections of genetic parts, the parts needed for a project and to organize them in project-specific libraries. (wikipedia.org)
  • citation needed] Array-based oligo-peptide scanning (sometimes called overlapping peptide scan or pepscan analysis): This technique uses a library of oligo-peptide sequences from overlapping and non-overlapping segments of a target protein, and tests for their ability to bind the antibody of interest. (wikipedia.org)
  • Restriction endonucleases and DNA ligase are then used to clone the sequences into bacterial plasmids. (wikipedia.org)
  • ligation
  • The digest products, now containing compatible sticky ends with each other (but incompatible sticky ends with themselves) are subjected to ligation, creating a new plasmid which contains the background elements of the original plasmid with a different insert. (wikipedia.org)
  • By doing this test, researchers can also determine the efficiency of the ligation and make adjustments as needed to ensure they get the desired number of clones for the library. (wikipedia.org)
  • fragment
  • The probability of covering a given location on the target with at least one fragment is therefore P = 1 − [ 1 − L G ] N . {\displaystyle P=1-\left[1-{\frac {L}{G}}\right]^{N}.} This equation was first used to characterize plasmid libraries, but it may appear in a modified form. (wikipedia.org)
  • This allows researchers to also determine the percentage of infectious viral particles actually carrying a fragment of the library. (wikipedia.org)
  • successfully
  • for more information see DNA ligase), the insert should become successfully incorporated into the destination plasmid. (wikipedia.org)
  • Calculating the titer allows researchers to approximate how many infectious viral particles were successfully created in the library. (wikipedia.org)
  • different
  • User workspace: Users can personalize their workspace by adding parts to the GenoCAD database, creating specialized libraries corresponding to specific design projects, and saving designs at different stages of development. (wikipedia.org)
  • Transformation of a bacterial host with two different plasmids is required. (wikipedia.org)
  • Origin
  • For instance, cancer origin of a library is often mixed with the tissue name (e.g., the tissue name "glioblastoma" indicates that the EST library was sequenced from brain tissue and the disease condition is cancer). (wikipedia.org)
  • Collection
  • In 1990 the plasmid collection of the Laboratory of Molecular Biology of Ghent University was added to the consortium (BCCM/GeneCorner). (wikipedia.org)
  • biological
  • In April, 2015, Peccoud and colleagues published a library of biological parts, called GenoLIB, that can be incorporated into the GenoCAD platform. (wikipedia.org)
  • The Reference Library System - sharing biological material and experimental data. (wikipedia.org)
  • database
  • In 1987 Hans Lehrach and Günther Zehetner founded the Reference Library / Primary Database (RLDB) at the Imperial Cancer Research Fund (ICRF) in London. (wikipedia.org)
  • construction
  • Variations on library construction have been produced by next-generation sequencing companies to suit their respective technologies. (wikipedia.org)
  • During the construction of the PET library, the fragments can be selected to all be of a certain size. (wikipedia.org)
  • regions
  • Accurate cloning of isolated fragments containing short tandem repeats, AT-rich DNA, or regions with strong secondary structure likewise can be difficult or impossible using conventional supercoiled plasmids. (aacrjournals.org)
  • short
  • There are now many companies that can create a fosmid library from any sample of DNA in a very short period of time at a relatively low cost. (wikipedia.org)
  • file
  • Importing/Exporting parts: Parts can be imported and exported individually or as entire libraries using standard file formats (e.g., tab delimited, FASTA, SBML). (wikipedia.org)
  • size
  • The likelihood of success in a directed evolution experiment is directly related to the total library size, as evaluating more mutants increases the chances of finding one with the desired properties. (wikipedia.org)