• ligation
  • The digest products, now containing compatible sticky ends with each other (but incompatible sticky ends with themselves) are subjected to ligation, creating a new plasmid which contains the background elements of the original plasmid with a different insert. (wikipedia.org)
  • By doing this test, researchers can also determine the efficiency of the ligation and make adjustments as needed to ensure they get the desired number of clones for the library. (wikipedia.org)
  • fragment
  • The probability of covering a given location on the target with at least one fragment is therefore P = 1 − [ 1 − L G ] N . {\displaystyle P=1-\left[1-{\frac {L}{G}}\right]^{N}.} This equation was first used to characterize plasmid libraries, but it may appear in a modified form. (wikipedia.org)
  • One single gene cluster, kaiABC, was found to be rhythmic in nature when the fragment of the plasmid responsible for rescue was sequenced. (wikipedia.org)
  • This allows researchers to also determine the percentage of infectious viral particles actually carrying a fragment of the library. (wikipedia.org)
  • generate
  • Furthermore, in vitro evolution experiments can generate far larger libraries (up to 1015) because the library DNA need not be inserted into cells (often a limiting step). (wikipedia.org)
  • insert
  • for more information see DNA ligase), the insert should become successfully incorporated into the destination plasmid. (wikipedia.org)
  • successfully
  • Calculating the titer allows researchers to approximate how many infectious viral particles were successfully created in the library. (wikipedia.org)
  • different
  • User workspace: Users can personalize their workspace by adding parts to the GenoCAD database, creating specialized libraries corresponding to specific design projects, and saving designs at different stages of development. (wikipedia.org)
  • Transformation of a bacterial host with two different plasmids is required. (wikipedia.org)
  • digest
  • Both the PCR product containing the mammalian gene with the new restriction sites and the destination plasmid are subjected to restriction digestion, and the digest products are purified by gel electrophoresis. (wikipedia.org)
  • Origin
  • For instance, cancer origin of a library is often mixed with the tissue name (e.g., the tissue name "glioblastoma" indicates that the EST library was sequenced from brain tissue and the disease condition is cancer). (wikipedia.org)
  • Collection
  • In 1990 the plasmid collection of the Laboratory of Molecular Biology of Ghent University was added to the consortium (BCCM/GeneCorner). (wikipedia.org)
  • biological
  • In April, 2015, Peccoud and colleagues published a library of biological parts, called GenoLIB, that can be incorporated into the GenoCAD platform. (wikipedia.org)
  • The Reference Library System - sharing biological material and experimental data. (wikipedia.org)
  • smaller
  • Negative selection normally precedes positive selection so that a smaller, purified prey library can be subjected to the more rigorous positive selection process. (wikipedia.org)
  • parts
  • In other cases, libraries correspond to the parts used for a particular design project. (wikipedia.org)
  • Parts can be moved from one library to another through a temporary storage area called the cart (analogous to e-commerce shopping carts). (wikipedia.org)
  • Importing/Exporting parts: Parts can be imported and exported individually or as entire libraries using standard file formats (e.g., tab delimited, FASTA, SBML). (wikipedia.org)
  • database
  • In 1987 Hans Lehrach and Günther Zehetner founded the Reference Library / Primary Database (RLDB) at the Imperial Cancer Research Fund (ICRF) in London. (wikipedia.org)
  • construction
  • Variations on library construction have been produced by next-generation sequencing companies to suit their respective technologies. (wikipedia.org)
  • During the construction of the PET library, the fragments can be selected to all be of a certain size. (wikipedia.org)