Leukemia k562 cells. Medical search. Frequent questions

K562 cells were the first human immortalised myelogenous leukemia cell line to be established. (wikipedia.org)
K562 cells are of the erythroleukemia type, and the cell line is derived from a 53-year-old female chronic myelogenous leukemia patient in blast crisis. (wikipedia.org)
Western blot analysis of Rab27 in human chronic myelogenous leukemia. (bdbiosciences.com)
Gamma-linolenic acid induces apoptosis and lipid peroxidation in human chronic myelogenous leukemia K562 cells. (omega-research.com)
In the current study, we tested the apoptotic effect of GLA on human chronic myelogenous leukemia K562 cells. (omega-research.com)
In this study, anticancer properties of heterocyclic salicylaldimines and their Cu(II) complexes on K562 human chronic myelogenous leukemia cell line were investigated. (ijpsonline.com)
Three studies have shown that monoclonal antibodies to the DENV fusion loop epitope reliably enhanced Zika virus infection (i.e., antibody-dependent enhancement [ADE]) in Fc receptor-bearing K-562 human myelogenous leukemia cells or the U-937 human monocytic cell line ( 1 - 3 ). (cdc.gov)
Compare clinical response, in terms of 1-year progression-free survival and rate of molecular complete remission, in patients with Philadelphia chromosome-positive chronic myelogenous leukemia (Ph+ CML) in chronic phase who have achieved a complete cytogenetic remission to single-agent tyrosine kinase inhibitor treated with interferon alfa and sargramostim (GM-CSF) vs tyrosine kinase inhibitor and GM-K562 cell vaccine. (knowcancer.com)
Freireich, E.J. Characteristics of accelerated disease in chronic myelogenous leukemia. (eurekaselect.com)

It led to loss of Δψm, release of cytochrome c, activation of caspases-9, -3 and cleavage of PARP, which suggested that bruceine D induced apoptosis of K562 cells through mitochondrial pathway. (edu.hk)
Flow cytometric analysis also demonstrated that GLA caused dose-dependent apoptosis of K562 cells. (omega-research.com)

Bruceine D exhibited potent cytotoxicity to K562 cells with IC50 of 6.37 ± 0.39 μM. (edu.hk)
GLA treatment could also elevate lipid peroxidation in K562 cells, and antioxidant alpha-tocopherol could reverse the cytotoxicity of GLA. (omega-research.com)
Extremely low-frequency magnetic fields significantly enhance the cytotoxicity of methotrexate and can reduce migration of cancer cell lines via transiently induced plasma membrane damage [med. (emf-portal.org)
He sought to determine how different levels of these antioxidants may affect oxidative activation of VP-16 in K562 human leukemia cells and the extent of cytotoxicity of VP-16 in these cells. (aicr.org)
Expanded natural killer cells killed both allogeneic and autologous primary myeloma cells avidly via a perforin-mediated mechanism in which the activating receptor NKG2D, natural cytotoxicity receptors, and DNAX-accessory molecule-1 played a central role. (haematologica.org)
The present study was undertaken to examine the mechanistic basis for the recent observation that the pyridine nucleotide derivative 6-aminonicotinamide (6AN, NSC 21206) enhances the accumulation and resulting cytotoxicity of cisplatin in a variety of tumor cell lines. (aspetjournals.org)
Our evaluation of biological activities, such as cytotoxicity against several cancer cell lines and antibacterial, antifungal, antimalarial, and mitochondrial inhibitory activities, demonstrated that the activity for each assay varies depending on the decalin configurations. (bvsalud.org)

We used primary acute myeloid leukemia (AML) samples and human AML cell lines to investigate the regulatory mechanisms of autophagy and its role in AML differentiation. (hindawi.com)
DNA (cytosine-5)-methyltransferase 3A (DNMT3A)-mutated acute myeloid leukemia (AML) has a poor prognosis, but the exact mechanism is still unclear. (frontiersin.org)
Acute myeloid leukemia (AML) is a common, heterogeneous, and aggressive hematopoietic malignancy, characterized by apparent genetic abnormalities ( 1 ). (frontiersin.org)

Furthermore, activation of autophagy allows to extend cell survival when exposed to different types of stressors such as starvation or cytotoxic drugs. (hindawi.com)
Rab27A is expressed in many human tissues and it regulates the secretion of several types of cellular vesicles, such as melanosomes, insulin granules of pancreatic beta cells, and lytic granules of cytotoxic T lymphocytes. (bdbiosciences.com)

The ability to induce these changes in K562 cell cycle and cell cycle regulation provides targets for cancer drugs. (wikipedia.org)
These results demonstrate that GLA could induce apoptosis in K562 cells. (omega-research.com)

Cell differentiation is induced by the deacetylase activity in these "undifferentiated progenitor cells," which alters the phenotype and morphology of the K562 cells. (wikipedia.org)
Apoptosis induction potential of each compound determined by mitochondrial membrane potential analysis (Rho123), cleaved caspase-3 expression analysis by flow cytometry and immunofluorescent staining and cell morphology analysis by giemza, hematoxylin and eosin and Papanicolaou protocols. (ijpsonline.com)
In the cell morphology analyzes, chromatin condensation and marginalization, changes in the cell membrane, ghost cells and apoptotic bodies were observed as evidence of apoptosis formation. (ijpsonline.com)
Morphology of the cells 6 days after G-CSF stimulation was assessed by Wright-Giemsa staining (bottom). (jci.org)
The antiproliferative effect of compounds was determined by WST-1 test, and nuclear morphology changes in cancer cells stained with Giemsa were evaluated. (japsonline.com)

The human erythroleukemia cell line K562 has been stably transfected to secrete GM-CSF. (acgtfoundation.org)

When the levels of these components are thrown off, they can either no longer inhibit apoptosis of the cancer cells, a role fulfilled by BCR/ABL, or they cause apoptosis to be induced, in the same vein as Bax and cytochrome C. These components are key in the mitochondria, and due to this, it has been supported that apoptosis uses the mitochondrial apoptosis pathway. (wikipedia.org)
Mitochondrial membrane potential was found at low levels in cells treated with Schiff base compounds and doxorubicin. (ijpsonline.com)

Embryonic stem cells isolation primarily requires the consent of donors and can include the killing of fertilized eggs. (techscience.com)
In 1907 the zoologist Ross Granville Harrison demonstrated the growth of frog embryonic cells that would give rise to nerve cells in a medium of clotted lymph . (wikipedia.org)

Biedler, J. L. & Riehm, H. Cellular resistance to actinomycin D in Chinese hamster cells in vitro: cross-resistance, radioautographic, and cytogenetic studies. (nature.com)
Thought to be disorders of hematopoietic stem cells, clonal cytogenetic abnormalities are frequently identified. (acgtfoundation.org)

In early phase clinical trials for both acute and chronic myeloid leukemias, we have observed the induction of anti-tumor immunity and associated clinical responses following K562/GM-CSF vaccination (see preliminary data). (acgtfoundation.org)
In this proposal, we seek to evaluate the integration of K562/GM-CSF vaccination with systemic therapies for MDS that alter host immunity and/or hematopoietic cellular differentiation. (acgtfoundation.org)
2. Conduct a clinical trial in MDS testing K562/GM-CSF vaccination integrated with the systemic agent(s) identified in aim 1 as being most active in combination with GM-CSF tumor vaccines (years 2 and 3). (acgtfoundation.org)

DNA, RNA, cDNA derived from ECACC mammalian cell lines allow screening for genes or expression patterns to identify lines most suitable for specific research. (sigmaaldrich.com)
These ATG genes are highly conserved in mammalian cells, allowing to study their functions also in higher eukaryotes [ 1 - 4 ]. (hindawi.com)
The degree of methylation affects the expression of target genes and has a significant impact on cell proliferation, apoptosis, cytokine secretion, and cell activity ( 6 , 7 ). (frontiersin.org)
In fact, many studies have demonstrated the oleuropein and its derivative have proved pharmacological activity against proliferation of cancer cells and several tumor cell lines by different mechanisms such arrest cell cycle and cause apoptosis in cancerous cells, by modulation of miRNA expression and upregulation and downregulation of several genes. (springer.com)
We applied NeoLoopFinder in Hi-C data from 50 cancer cell lines and primary tumors and identified tens of recurrent genes associated with enhancer hijacking. (nature.com)
18,21 Early in 2010, a team of Taiwanese researchers discovered that GLA regulates the inflammatory "master molecule" nuclear factor-kappaB or NF-kB, preventing it from switching on genes for inflammatory cytokines in cell nuclei. (lifeextension.com)

They can inhibit the growth of tumor cells, kill them via phagocytosis, and promote anti-tumor immunity. (frontiersin.org)

B ) Expression of ASXL1-WT and its mutants in 293T cells or 32Dcl3 cells transiently transfected or stably transduced with a FLAG-ASXL1-MT1, ASXL1-MT2, ASXL1-WT, or an empty vector (pMYs-IG). (jci.org)

Many factors and components play a role in the cell cycle of K562 cells in terms of growth, cell differentiation, and apoptosis. (wikipedia.org)
The growth of these leukemic cells are controlled by either initiating cell differentiation or apoptosis to occur. (wikipedia.org)
This gene targets the cyclin-dependent kinase inhibitor, p21, and causes cell differentiation, cell cycle arrest in G1, and ultimately apoptosis. (wikipedia.org)
Other methods being focused on in the regulation of K562 cells include therapeutic methods like polyphyllin D, which caused differentiation from the progenitor state to occur, and for apoptosis to begin. (wikipedia.org)
Abaza L, Talorete TP, Yamada P, Kurita Y, Zarrouk M, Isoda H (2007) Induction of growth inhibition and differentiation of human leukemia HL-60 cells by a Tunisian gerboui olive leaf extract. (springer.com)
ASXL1 mutations inhibit G-CSF-induced myeloid differentiation of 32Dcl3 cells. (jci.org)
Mesenchymal stem cells (MSCs) are multipotent stromal cells capable of self-renewal and exhibit multilineage differentiation properties. (techscience.com)
Enhanced expression of IL-6, IGF-1, TGF-β, and VEGF has also been observed in LC treated BMSCc+ groups, suggested the cardiac differentiation of BMSCc+, and can be utilized in tissue engineering for cardiac cell therapy. (techscience.com)

The cells are non-adherent and rounded, are positive for the bcr:abl fusion gene, and bear some proteomic resemblance to both undifferentiated granulocytes and erythrocytes. (wikipedia.org)
There are many different cellular components involved in the cycle of apoptosis such as BCR/ABL, Bcl-2, Bax protein, and cytochrome C. The tumor suppressor gene p53 is also important in the cell cycle regulation of K562 cells. (wikipedia.org)
Gene expression in benzene-exposed workers by microarray analysis of peripheral mononuclear blood cells: induction and silencing of CYP4F3A and regulation of DNA-dependent protein kinase catalytic subunit in DNA double strand break repair. (cdc.gov)
To analyze benzene-caused aberrant gene expression, we examined differential gene expression by microarray analysis of peripheral mononuclear blood cells from seven workers diagnosed with benzene poisoning and seven matched controls. (cdc.gov)
details] The synergic effects of Crocus sativus L. and low frequency electromagnetic field on VEGFR2 gene expression in human breast cancer cells [med. (emf-portal.org)
Strategies to circumvent resistance involve CRISPR/Cas9 gene editing to restore proper RNA splicing function in resistant cells. (osu.edu)
We tested whether natural killer cells expanded by co-culture with K562 cells transfected with 41BBL and membrane-bound interleukin-15 could kill myeloma cells with a high-risk gene expression profile in vitro and in a unique model which recapitulates human myeloma. (haematologica.org)
Antibody Target: POL24H8 antibody targetdescription: This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes antibody vendorname: Abcam antibody vendorid: ab5408 age: immortalized age description: Immortal cells control: Input control description: Control signal which may be subtracted from experimental raw signal before peaks are called. (nih.gov)
Chronic myeloid leukemia (CML) is a myeloproliferative disease caused due to translocation between chromosome 9 and 22 leading to a chimeric gene product known as Bcr-Abl. (eurekaselect.com)

We have developed a genetically modified tumor cell vaccine for the treatment of myeloid malignancies. (acgtfoundation.org)
Arm II: Patients receive GM-K562 cell vaccine intradermally once every 3 weeks for a minimum of 6 months. (knowcancer.com)
The injectable polio vaccine developed by Jonas Salk was one of the first products mass-produced using cell culture techniques. (wikipedia.org)
This vaccine was made possible by the cell culture research of John Franklin Enders , Thomas Huckle Weller , and Frederick Chapman Robbins , who were awarded a Nobel Prize for their discovery of a method of growing the virus in monkey kidney cell cultures. (wikipedia.org)

Human and mouse T cells treated with ITFG1 in vitro secreted the cytokines IFN-g, TNF-a and IL-10, while in vivo ITFG1 was protective in a mouse acute graft-versus-host disease (GVHD) model. (rndsystems.com)
The DNMT3A-mutated AML cells attenuated M1 macrophage polarization and resisted its killing effect in vitro and in vivo . (frontiersin.org)
The effect of pulse repetition frequency on the uptake into electropermeabilized cells in vitro with possible applications in electrochemotherapy [med. (emf-portal.org)
Cell-based and in vitro assays show that Retro-2 blocks delivery of newly-synthesized TA-proteins to the ER-targeting factor ASNA1 (TRC40). (elifesciences.org)
We established a monoclonal antibody (MAb), N6HB426-20, that recognizes the extracellular domain of human NTCP and blocks HBV entry in vitro into human liver cells but has much less of an inhibitory effect on bile acid uptake. (bvsalud.org)

The myelodysplastic syndromes (MDS) are a group of clonal neoplastic hematologic disorders characterized by varying degrees of bone marrow failure, abnormal hematopoiesis, and proliferation of myeloid blast cells. (acgtfoundation.org)
After treatment with β-iminophosphonate (E,Z)(3-Methyl-2-(pyridin2-ylimino)butyl)diphenylphosphine oxide (4e) proliferation/viability of cancer cells was strongly inhibited and in 65.61% of the cells signs of early apoptosis were observed. (japsonline.com)

These changes can also drive the leukemic cells to a state of stress, which allows for increased sensitivity of the cells to drugs that initiate apoptosis. (wikipedia.org)

DNA-PKcs mRNA was found consistently increased in the patients and DNA-PKcs mRNA and protein were induced by hydroquinone in HL-60 cells. (cdc.gov)
Multidrug resistance parameters, tissue infiltration parameters, receptors for colony-stimulating factors (CSFr) and cell cycle parameters were analyzed using flow cytometry in 145, 109 initial and 36 relapsed or refractory, acute nonlymphoblastic leukemia (ANLL) patients to find out clinically more reliable functional parameters. (karger.com)
Leukemia cells in patients are intrinsically resistant to standard chemotherapy, and resistance development in leukemia cells toward drugs results in treatment failure[ 4 , 5 ]. (ijpsonline.com)
Evidence exists that immune activation against hematopoietic elements frequently occurs in MDS patients, based on the identification of lymphocytic infiltrates in the marrow, oligoclonal expansion of T cells, and excessive production of tumor necrosis factor alpha. (acgtfoundation.org)
Results Natural killer cells from healthy donors and myeloma patients expanded a median of 804- and 351-fold, respectively, without significant T-cell expansion. (haematologica.org)
1 Significant advances have been made by combining novel agents with autologous peripheral blood stem cell transplantation which allows for long-term disease-free survival in the majority of transplant-eligible patients. (haematologica.org)
In particular, ASXL1 mutations are common in patients with hematologic malignancies associated with myelodysplasia, including myelodysplastic syndromes (MDSs), and chronic myelomonocytic leukemia. (jci.org)
In such cases, stem cell transplant therapy, which is limited by donor availability, is the only proven cure for the patients. (eurekaselect.com)

Dano, K. Active outward transport of daunomycin in resistant Ehrlich ascites tumor cells. (nature.com)

The change in phenotype induces a decrease in the growth rate and leads the K562 cells to the terminal path of becoming mature erythroids, monocytes, and mature macrophages. (wikipedia.org)

Inhibiting these is an important regulation mechanism of cancer, because it prevents cells from progressing into mitosis. (wikipedia.org)
Apoptosis is an important mechanism in regulating K562 cells and can be induced by the changes in the metabolic state of the cells. (wikipedia.org)
The specific mechanism of promoting tumor cell metastasis by high c-Myc expression remains to be explained. (bvsalud.org)

Typical apoptotic nuclei were shown by staining of K562 cells with DNA-binding fluorochrome Hoechst 33342, characterized by chromatin condensation and nuclear fragmentation. (omega-research.com)
However, the applicability of such traditional chemotherapeutic approaches is primarily limited due to acquired Multi-Drug Resistance (MDR) in cells, which is acquired through the production of anti-apoptotic cascades and the overexpression of cell membrane-based drug efflux pumps, such as P-glycoprotein, for self-defense. (ijpsonline.com)
Pulsed electromagnetic field with temozolomide can elicit an epigenetic pro-apoptotic effect on glioblastoma T98G cells [med. (emf-portal.org)
Asgharzade S, Sheikhshabani SH, Ghasempour E, Heidari R, Rahmati S, Mohammadi M, Jazaeri A, Amini-Farsani Z (2020) The effect of oleuropein on apoptotic pathway regulators in breast cancer cells. (springer.com)

Since NHEJ is error-prone, induction of DNA-PKcs and NHEJ may contribute to mutagenesis and leukemia by benzene. (cdc.gov)

K562 cells are part of the NCI-60 cancer cell line panel used by the National Cancer Institute. (wikipedia.org)
Another important group of regulators of the K562 line are Sirtuins, referred to as SIRTS. (wikipedia.org)
The Leukemia-Lymphoma Cell Line Factsbook. (wikipedia.org)
This cell line has been recommended as a control cell line for use with the Telomere PNA Kit/FITC for flow cytometry from DakoCytomation. (sigmaaldrich.com)
Lysate from the K-562 cell line (Cat. (bdbiosciences.com)
SP1 and GATA3) and HDAC2-GATA1 regulatory networks on rescued regions in K562 cell line. (biomedcentral.com)
Anticancer activity on human fibrosarcoma cell line HT 1080 was assessed. (japsonline.com)
Among tested compounds β-iminophosphonate '(E,Z)(3-Methyl-2-(pyridin-2-ylimino)butyl) diphenylphosphine oxide (4e) showed anticancer activity against human fibroblastoma cell line HT 1080. (japsonline.com)

Chronic myeloid leukemia (CML), an acquired malignant myeloproliferative disorder of hematopoietic stem cells, is one of the three most common forms of leukemia. (edu.hk)
Leukemia is a blood cancer that develops in the hematopoietic system and presents in the bone marrow with or without peripheral blood cell involvement[ 1 , 2 ]. (ijpsonline.com)

This arrest leads to "shrinkage, blebbing, nuclear fragmentation, chromatin condensing" and other morphological changes that cause the cell to program death at this point. (wikipedia.org)
lab: PSU-m lab description: Hardison - Penn State University datatype: ChipSeq datatype description: Chromatin IP Sequencing cell: MEL cell organism: mouse cell description: Leukemia (K562 analog) cell sex: U antibody: Pol2-4H8 antibody antibodydescription: Mouse monoclonal to RNA polymerase II CTD repeat YSPTSPS antibody (4H8) - ChIP Grade. (nih.gov)

Conclusions These studies provide the rationale for testing expanded natural killer cells in humans. (haematologica.org)

The offset of these cellular components from their balance point causes morphological changes, which result in the K562 cells being arrested in the G2/M phase of the cell cycle. (wikipedia.org)
These play a role in cellular stress, metabolism, and autophagy, by interacting with deacetylases activity in the cell. (wikipedia.org)
Basal macroautophagy (thereafter referred to as autophagy), a catabolic recycling system in cells, is key to maintaining cellular homeostasis and survival. (hindawi.com)
Leukemias were originally termed acute or chronic based on life expectancy but now are classified according to cellular maturity. (curehunter.com)
The Mesenchymal and Tissue Stem Cell Committee, which belongs to the International Society for Cellular Therapy, stated that plastic-adherent properties are the minimum criteria for determining MSCs. (techscience.com)

Cancer cells frequently display intrinsic or acquired resistance to chemically diverse anticancer drugs, limiting therapeutic success. (nature.com)
Chemotherapy is one of the most important treatment options in the treatment of all types of leukemia, but multi-drug resistance often precludes the success of this treatment. (ijpsonline.com)
CRISPR/Cas9 Genome Editing of the Human Topoisomerase II Intron 19 5' Splice Site Circumvents Etoposide Resistance in Human Leukemia K562 Cells. (osu.edu)
hsa-miR-9-3p and hsa-miR-9-5p as Post-Transcriptional Modulators of DNA Topoisomerase II in Human Leukemia K562 Cells with Acquired Resistance to Etoposide. (osu.edu)

The leaf extracts and its derivative compounds have shown potent anticancer effects against different cell lines in different organs. (springer.com)

Cabozantinib versus everolimus in advanced renal-cell carcinoma. (eurekaselect.com)

Internal control cell in telomere measurements performed by flow cytometry. (sigmaaldrich.com)

GLA induced K562 cell death in a dose-dependent manner. (omega-research.com)
The transferred, expanded natural killer cells proliferated in vivo in an interleukin-2 dose-dependent fashion, persisted up to 4 weeks, were readily detectable in the human bone, inhibited myeloma growth and protected bone from myeloma-induced osteolysis. (haematologica.org)

The organ tropism is different depending on the infiltration parameters, vascular cell adhesion molecule to splenomegaly, matrix metalloprotease-2 to hepatomegaly and to extramedullary infiltration other than spleen, liver or lymph node. (karger.com)

Autophagy is an intracellular degradation system that ensures a dynamic recycling of a variety of building blocks required for self-renewal, homeostasis, and cell survival under stress. (hindawi.com)
While the importance of autophagy for cell homeostasis and survival has long been appreciated, its role in tumorigenesis and cancer progression is still developing [ 10 , 11 ]. (hindawi.com)
Static or 50 Hz magnetic fields at 100 µT do not modify the clonogenic survival of doxorubicin-treated MCF-7 cancer cells [med. (emf-portal.org)
Data used for survival analysis in leukemia and gastric cancer were downloaded from the cBioPortal for Cancer Genomics ( https://www.cbioportal.org ) 45 . (nature.com)

Multiple myeloma (MM) is a malignant plasma cell disorder with debilitating symptoms related to anemia, immunosuppression, bone destruction, and renal failure. (haematologica.org)

However, it can be challenging to differentiate when they are connected with wounded tissue, opposed to when they instruct tissue-specific progenitor cells responsible for the redevelopment of damaged tissue. (techscience.com)

C ) HEK293T cells were transiently transfected with FLAG-ASXL1-MT, FLAG-ASXL1-WT, and Myc-EZH2 cDNA, followed by IP of FLAG epitope and Western blotting for EZH2. (jci.org)

These mice are devoid of endogenous natural killer and T-cell activity and were used to determine whether adoptively transferred expanded natural killer cells could inhibit myeloma growth and myeloma-associated bone destruction. (haematologica.org)
Mesenchymal stem cells (MSCs) are multipotent cells usually isolated from bone marrow, endometrium, adipose tissues, skin, and dental pulp. (techscience.com)

23 PPARs are intracellular receptors that modulate cell metabolism and responses to inflammation. (lifeextension.com)

Flow cyt: U87,and HeLa cells. (abcam.com)
F ) Surface expression of CD11b in 32Dcl3 cells transduced with indicated plasmid after incubation with 1 ng/ml IL-3 (blue) or 50 ng/ml G-CSF for 6 days (red) was analyzed by flow cytometry (top). (jci.org)

Cell lysates were also subject to immunoblotting with anti-FLAG Ab or anti-Myc Ab. (jci.org)

Resistin significantly promoted the expression of inflammatory proteins in AML cells. (frontiersin.org)
Cyclins and cyclin-dependent kinases (cdks) are evolutionarily conserved proteins that are essential for cell-cycle control in eukaryotes. (bdbiosciences.com)
The main cyclin-cdks complexes formed in vertebrate cells are cyclin D-cdk4 (G0/G1), cyclin E-cdk2 (G1/S), cyclin A-cdk2 (S) and cyclin B1-cdk1 (G2/M). These complexes are regulated by activating and inhibitory phosphorylation events, as well as by interactions with small regulatory proteins including p21 and p27Kip1. (bdbiosciences.com)

Integrin-alpha FG-GAP repeat-containing protein 1 (ITFG1), also known as T cell immunomodulatory protein (TIP), was initially identified using bioinformatics and high-throughput cell-based screening assays to isolate novel factors involved in T cell biology. (rndsystems.com)
In cancer biology, controlling leukemia remains a big challenge. (ijpsonline.com)
MSCs played a crucial role in regenerative therapy and have been introduced as an interdisciplinary field between cell biology and material science. (techscience.com)
Holyoake, T.L. A comparison of normal and leukemic stem cell biology in chronic myeloid leukemia. (eurekaselect.com)
Since Haberlandt's original assertions, methods for tissue and cell culture have been realized, leading to significant discoveries in biology and medicine. (wikipedia.org)

The problem with K562 cells, and many other cancer cell types, is an overabundance of Aurora kinases. (wikipedia.org)
Synergy of micro and nanosecond electrical pulses with chemotherapeutics on human cancer cell viability [med. (emf-portal.org)
Among others Oleuropein and its derivative compounds (hydroxytyrosol, tyrosol and others) were studied in a several types of cancer cells. (springer.com)
Fig. 2: Detection of neoloops in 50 cancer cell lines or patient samples. (nature.com)
When A549 lung cancer cells or K562 leukemia cells were treated with 62.5 μM 6AN for 21 h and then pulse-labeled with [ 35 S]methionine for 1 h, increased labeling of five polypeptides, one of which corresponded to a M r ∼78,000 glucose-regulated protein (GRP78), was observed. (aspetjournals.org)

MTT assay was used to evaluate cell growth inhibition. (edu.hk)
Adoptive transfer of expanded natural killer cells inhibited the growth of established OPM2 and high-risk primary myeloma tumors grown in the murine model. (haematologica.org)

K562 cells can spontaneously develop characteristics similar to early-stage erythrocytes, granulocytes and monocytes and are easily killed by natural killer cells as they lack the MHC complex required to inhibit NK activity. (wikipedia.org)

moreover, CYP4F3A mRNA and protein were induced by benzene metabolite phenol in HL-60 and K562 cells as well as ex vivo in human peripheral neutrophils. (cdc.gov)

Different approaches to evaluate the future of biomaterials and stem cell properties have been developed. (techscience.com)

Western Blot: PARP Antibody (194C1439) - Cleaved [NB100-56599] - Analysis of cleaved PARP in staurosporine-treated Jurkat cells at various time points, antibody at 2 ug/mL. (novusbio.com)
Immunocytochemistry/ Immunofluorescence: PARP Antibody (194C1439) - Cleaved [NB100-56599] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. (novusbio.com)
The poly (ADP-ribose) polymerase (PARP) is involved in cell recovery from DNA damage, such as methylation of N3-adenine, which activates the base excision repair process. (novusbio.com)

Whether this represents a secondary event in response to cell injury and the generation of neo-antigens, or an initiating event inducing immunopathology, remains controversial. (acgtfoundation.org)

Anatomy23

Organisms18

Diseases52

Chemicals and Drugs65

Analytical, Diagnostic and Therapeutic Techniques and Equipment25

Phenomena and Processes38

Disciplines and Occupations1

Information Science4

Health Care4

Myelogenous9

Apoptosis of K562 cells2

Cytotoxicity7

Acute myeloid3

Cytotoxic2

Induce2

Morphology5

Erythroleukemia1

Mitochondrial2

Embryonic2

Cytogenetic2

Vaccination3

Genes6

Anti-tumor immunity1

Stably1

Differentiation8

Gene9

Vaccine4

Vitro5

Proliferation2

Leukemic cells1

Patients8

Tumor cells1

Phenotype1

Mechanism3

Apoptotic4

Induction1

Line8

Hematopoietic2

Chromatin2

Conclusions1

Cellular5

Resistance4

Potent1

Renal-cell carc1

Performed by flow cytometry1

Dose-dependent2

Lymph1

Survival4

Malignant1

Progenitor cells1

EZH21

Bone2

Responses1

Flow2

Lysates1

Proteins3

Biology5

Cancer5

Growth2

Characteristics1

Vivo1

Stem Cell1

PARP3

Secondary1