AtherosclerosisAtherosclerotic lesionsAutosomal dominantNeuronsC57BLExpressionProliferationAorticTarget genesDouble knockoutApoeVascular lesionsProinflammatory genesMacrophagesTransgenic mouseOverexpressionHumansPathwaysReceptorGeneticMutations in the geneMolecularProteinDeficientRetinalProgressionNeuroscienceAxonalSerumFetalMutationDependentVivoCircadianEarly-onsetImmuneKnock outSpinalPeripheral bloodChronicStimulationDeficiencyPhenotypesSignificantlyAlzheimer'sProteinsModelBrainCellMRNAMitochondrialDevelopment
Atherosclerosis20
- In mice, blocking ceramide production improves insulin sensitivity, prevents β-cell failure, resolves hepatic steatosis, hypertriglyceridemia, and prevents atherosclerosis, and heart failure ( 6 - 22 ). (frontiersin.org)
- Chang has spent many years studying the role that the gene ACAT1 plays in multiple human diseases, including Alzheimer's and atherosclerosis, a hardening of the arteries due to buildup of plaque. (dartmouth.edu)
- A buildup of cholesterol esters in macrophages (white blood cells) within an aortic lesion is a hallmark of atherosclerosis. (dartmouth.edu)
- Her research involves the use of atherosclerosis mouse models engineered to be born with or without the ACAT1 gene in their macrophages. (dartmouth.edu)
- We are putting macrophage-specific ACAT1-knockout mice on a high cholesterol diet to induce atherosclerosis," Melton explains. (dartmouth.edu)
- In vivo, a high-fat-diet-induced atherosclerosis model was established in apolipoprotein E knockout mice. (bvsalud.org)
- We then analyzed diet-induced atherosclerosis and spontaneous lesion formation in the Mef2a and Mef2a+/- Apoe-/- mice. (csuohio.edu)
- Accelerated atherosclerosis growth in C57Bl6 mice by overexpressing AAV-mediated PCSK9 and partial carotid ligation. (aabioetica.org)
- Finding out the position of a selected gene in atherosclerosis usually requires a time-consuming and sometimes tough means of producing double knockouts or transgenics on ApoE-/- or LDL receptor (LDLR)-/- background. (aabioetica.org)
- Nonetheless, utilizing this methodology in C57BL6 wild-type (C57) mice, it took ~three months to develop atherosclerosis. (aabioetica.org)
- Our partial carotid ligation mannequin is used to quickly develop atherosclerosis by inducing disturbed movement within the left frequent carotid artery inside 2 weeks in ApoE-/- or LDLR-/- mice. (aabioetica.org)
- Right here, we mixed these two approaches to develop an accelerated mannequin of atherosclerosis in C57 mice. (aabioetica.org)
- This led us to investigate whether FXI deficiency in elderly ApoE knockout (KO) mice would decrease pathological alterations compatible with atherosclerosis and AD. (spandidos-publications.com)
- However, neither atherosclerosis nor AD‑associated pathological alterations in the elderly mice brains were observed in either the DKO mice or the ApoE KO mice. (spandidos-publications.com)
- Therefore, the ApoE KO and DKO mice cannot serve as mouse models for studying AD or pathological brain changes compatible with atherosclerosis. (spandidos-publications.com)
- iii) In a transgenic mouse model of AD (B6Tg2576), early atherosclerosis lesions were detected and were positively correlated with cerebral β amyloid deposits when mice were fed a normal diet ( 11 ) or atherogenic diets ( 12 ). (spandidos-publications.com)
- vi) APP transgenic mice with apolipoprotein E (ApoE) deficiency had increased atherosclerosis and vascular inflammation ( 16 ). (spandidos-publications.com)
- they decrease tissue damage and arrhythmia in myocardial infarction, may reduce progression of atherosclerosis (CB 2 receptor stimulation inhibits lesion progression), and FAAH knockout mice (which have enhanced endocannabinoid levels) show decreased cardiac dysfunction with age compared to wild-types. (thecannabisadvisory.com)
- Objective: The objective of the study was to investigate the effect of fetal programming on the development of atherosclerosis in the offspring in a mouse model. (utmb.edu)
- Conclusions: There is a strong effect of fetal programming on the development of atherosclerosis in the apoE mouse model. (utmb.edu)
Atherosclerotic lesions4
- Hematoxylin-eosin, oil red O, and Sirius red staining were performed for the determination of atherosclerotic lesions. (bvsalud.org)
- In vivo, the specific overexpression of miR-99a-5p significantly reduced atherosclerotic lesions, accompanied by a significant downregulation of autophagy marker CD68 protein expression. (bvsalud.org)
- Analysis of macrophage scavenger receptor (SR-A) expression in human aortic atherosclerotic lesions. (ox.ac.uk)
- We also establish that human SR-A is expressed by tissue macrophages in liver and lung and by macrophage-derived foam cells within aortic atherosclerotic lesions, with little detectable expression by smooth muscle cells or aortic endothelium. (ox.ac.uk)
Autosomal dominant1
- Familial AD (FAD) cases are caused by autosomal dominant mutations in the genes for amyloid precursor protein (APP) and the presenilins (PS1 and PS2) ( Sisodia and St George-Hyslop, 2002 ). (jneurosci.org)
Neurons9
- examined the proliferation, survival, and differentiation of newborn neurons in the dentate gyrus of mice deficient in BACE1 . (eneuro.org)
- The reduced number of differentiated neurons in the dentate gyrus of BACE1 -/- mice was not countered by increased differentiation of NPCs into astrocytes or oligodendrocytes. (eneuro.org)
- BACE1 -/- mice displayed increased NPC proliferation, but overall, these cells do not mature into neurons that could potentially compensate for the learning and memory defects associated with AD. (eneuro.org)
- The relevance of Cln8 up-regulation in hippocampal neurons of kindled mice should be further explored. (biomedcentral.com)
- Those studies used mice lacking serotonergic neurons resulting from the loss of function mutations in the gene encoding the LIM homeobox transcription factor 1 ( Lmx1b ). (aspetjournals.org)
- Here we demonstrate that similar activity amplification occurs in mice, and that this is related to suppressed inhibition to PB neurons from the central nucleus of the amygdala (CeA) in animals of either sex. (iasp-pain.org)
- We show that-in both rats and mice-PB contains few GABAergic neurons, and that most of its GABAergic inputs arise from CeA. (iasp-pain.org)
- Additionally, parvalbumin-expressing neurons and Npas1-expressing neurons were distinct in their autonomous and driven firing characteristics, their expression of intrinsic ion conductances, and their responsiveness to chronic 6-hydroxydopamine lesion. (nih.gov)
- In paper №2, I describe how mesDA neurons transplanted in the adult SN of a PD mouse model, extended axons across millimetres into the striatum, functionally reforming the nigrostriatal pathway. (lu.se)
C57BL2
- After induction of EAE, C57BL/6 mice were fed on a HSD (4.0% NaCl) or normal diet (0.4% NaCl). (neurology.org)
- To control for genetic background variability, we compared the original phenotype with that of single Ccl2, Cx3cr1 and Ccl2/Cx3cr1 double knockout mice obtained from backcrosses of CCDKO with C57Bl/6 mice. (ox.ac.uk)
Expression32
- In addition, an excess of oxidative free radicals also increases the expression of proinflammatory genes and activates the inflammation process [ 18 , 19 ]. (hindawi.com)
- During his three-and-a-half years at Columbia, Tsien identified a handful of rat genes whose expression is regulated by brain activity - work Kandel calls "some very beautiful science. (the-scientist.com)
- When you see genes whose expression goes up or down in response to stimulation, what does that really mean? (the-scientist.com)
- METHODS: In this study, we first analyzed the expression of PLCL1 in the synovial tissue of RA patients and K/BxN mice by immunohistochemical staining. (bvsalud.org)
- At the molecular level, MWCNT exposure significantly increased the expression of the cell proliferation markers Ki-67 and PCNA and a panel of cell cycle-controlling genes in the lungs in a TIMP1- dependent manner. (cdc.gov)
- Under salt stress conditions, Arabidopsis lines expressing VuWRKY21 or VuWRKY87 showed elevated expression of genes participating in saline stress response pathways and reduced oxidative stress induced by reactive oxygen species (ROS). (mdpi.com)
- Among the salt-responsive genes in Arabidopsis , AtP5CS1 , AtNHX1 , AtRD29A , AtSOS3 , AtSOS2 , and AtSOS1 exhibited modulated expression levels after stress imposition. (mdpi.com)
- These findings suggest that the expression of both VuWRKY genes in Arabidopsis , particularly VuWRKY21 , activated genes involved in salinity tolerance. (mdpi.com)
- Thus, PARP-1 might plays a critical role as a signaling molecule, which controls the expression of multiple genes involved in the inmmune response. (imim.es)
- Knockout of Bmal1 in mouse OPCs during development disrupts the expression of genes associated with circadian rhythms, proliferation, density, morphology, and migration, leading to changes in OPC dynamics in a spatiotemporal manner. (stanford.edu)
- Patient biopsies revealed an 89% increase in CD47 expression by immunohistochemistry in metastatic brain TNBC tumors compared to primary lesions. (sitcancer.org)
- In mice bearing brain metastatic 4T1br3 tumors, anti-CD47 treatment shrank tumors by roughly 50% compared to controls, which accompanied by a 5-fold increase in expression of F4/80 macrophage markers in the tumors. (sitcancer.org)
- Notably, PNS injury triggers a coordinated regenerative gene expression programme. (nature.com)
- However, the molecular link between retrograde signalling and the regulation of this gene expression programme that leads to the differential regenerative capacity remains elusive. (nature.com)
- However, the final link between axonal injury-induced retrograde signalling and the regulation of essential regenerative gene expression remains elusive. (nature.com)
- In search of key regulatory mechanisms that may clarify the molecular nature of this regenerative gene expression programme, we hypothesized that as an 'orchestrator of gene regulation' epigenetic changes would direct expression of genes crucial for regeneration only in the presence of pro-regenerative signalling following peripheral but not central damage. (nature.com)
- In addition, we observed that extracellular signal-regulated kinase (ERK) axonal retrograde signalling is required for PCAF-dependent acetylation at these promoters and for their enhancement in gene expression. (nature.com)
- Our results show the first evidence of immediate retrograde signalling leading to long-term epigenetic reprogramming of gene expression of select genes whose modulation leads to axonal regeneration in the hostile spinal environment. (nature.com)
- We serendipitously observed the de novo expression of PV in ependymal cells of the lateral ventricle wall following in vivo lesioning and brain slicing for the preparation of organotypic hippocampal slice cultures (OHSCs). (unifr.ch)
- Indeed, in vivo stab injury enhances PV-expression in ependymal cells adjacent to the lesion, whereas neuraminidase denudation is without effect. (unifr.ch)
- A fragment of the Dab2 human cDNA was also isolated based on its frequent loss of expression in ovarian cancer, and was termed DOC-2 (Differentially expressed in ovarian carcinoma gene 2) [ 2 ]. (biomedcentral.com)
- Their signaling pathways are not fully elucidated but they can lead to changed expression of a variety of genes, including those involved in inflammatory responses. (thecannabisadvisory.com)
- Increased gene expression levels for IFN-γ, IL-4 and IL-10 in new-onset patients from at-risk patients might be used as potential markers for progression of the disease. (indexindex.com)
- Significant downregulation of beta-defensin1 gene (DEFB1) expression was observed when human bronchial epithelial cells (HBEpCs) were exposed to IAV. (cdc.gov)
- Pansensitive and panresistant genes to 21 NCCN-recommended drugs with concordant mRNA and protein expression were identified. (cdc.gov)
- Angiopoietin 1 and 2 are antagonizing, with Ang-1 (produced in podocytes) promoting vessel integrity, inhibiting vascular leakage and inflammatory gene expression. (ajkdblog.org)
- Analysis of gene expression revealed a shift towards Th17 helper T cell driven autoimmunity by HSD. (neurology.org)
- In vitro, restimulation of MOG primed splenocytes from mice on a HSD led to increased expression of IL-17 and Th17 signature genes. (neurology.org)
- Here we characterized the spatial and temporal expression of Cln8 mRNA in mice. (biomedcentral.com)
- Alternative polyadenylation (APA), a posttranscriptional mechanism of gene expression via determination of 3′UTR length, has an emerging role in carcinogenesis. (jci.org)
- This is not surprising given the strong evidence for interaction between myelin and axon gene expression in development and after experimental nerve lesions. (medscape.com)
- TCDD for every step of the mechanism described for 2,3,7,8-TCDD carcinogenesis in humans including receptor binding, gene expression, protein activity changes, cellular replication, oxidative stress, promotion in initiation-promotion studies and complete carcinogenesis in laboratory animals. (who.int)
Proliferation3
- Increased proliferation in BACE1 -/- mice only was also confirmed by immunolabeling with an anti-Ki67 antibody, a marker of cellular proliferation. (eneuro.org)
- These results indicate that complete, but not partial loss, of BACE1 activity increases cell proliferation in the dentate gyrus of young adult mice. (eneuro.org)
- Experimentally confirmed target genes were identified for the 73 diagnostic miRNAs, from which proliferation genes were selected from CRISPR-Cas9/RNA interference (RNAi) screening assays. (cdc.gov)
Aortic2
- We previously demonstrated that factor XI (FXI) deficiency in mice reduced the atherosclerotic plaque area in coronary sinuses and the aortic arch. (spandidos-publications.com)
- The ApoE KO mice developed an advanced atherosclerotic lesion area in the aortic arch, which was reduced by 33% in the DKO mice. (spandidos-publications.com)
Target genes4
- Nrf2 activates transcription of antioxidant enzymes by binding to the antioxidant response element (ARE) in the promoter regions of its target genes [ 23 , 24 ]. (hindawi.com)
- Upon exposure to ROS, Keap1 undergoes a conformational change that allows Nrf2 to translocate to the nucleus, bind to the ARE region, and initiate transcription of target genes [ 24 ]. (hindawi.com)
- Oxidized Keap1 dissociates from Nrf2, allowing Nrf2 to translocate to the nucleus, bind to the ARE region, and initiate transcription of target genes. (hindawi.com)
- Bioinformatic analysis was used to predict the target genes of miR-99a-5p. (bvsalud.org)
Double knockout1
- Ccl2/Cx3cr1 double knockout (CCDKO) mice show an early onset retinal degeneration and have been suggested as a model for AMD. (ox.ac.uk)
Apoe6
- To further investigate the role of MEF2A in atherogenesis, we studied knockout mice targeted to the mouse Mef2a gene and created a novel Mef2a+/- Apoe-/- knockout mouse strain. (csuohio.edu)
- A total of 47 Mef2a +/+, Mef2a+/- mice on Apoe-/- knockout background, including 26 males (15 wild type and 11 heterozygous) and 21 females (9 wild type and 12 heterozygous) were maintained on a Western-type diet for 12 weeks. (csuohio.edu)
- The present study used ApoE/factor XI double KO (ApoE/FXI DKO) mice aged 64 weeks and age‑matched ApoE KO mice to serve as a control group. (spandidos-publications.com)
- The human ApoE gene has 3 allelic variants (ε2, ε3, and ε4). (spandidos-publications.com)
- In mice, the association between ApoE deficiency and AD is not clear ( 17 - 26 ). (spandidos-publications.com)
- Study Design: Male and female mice of the wild type and the knockout for the apoprotein E (apoE) gene were cross-bred to obtain all 4 possible genetic offspring types. (utmb.edu)
Vascular lesions2
- There is significant infiltration of immune cells into occlusive vascular lesions in humans and in animal models of PAH, but no knowledge of inflammatory gene silencing by DNA modifications. (mergullo.net)
- RPE and vascular lesions develop secondarily with increasing penetrance with age and are clinically similar to retinal telangiectasia not to choroidal neovascularisation. (ox.ac.uk)
Proinflammatory genes1
- For example, there is no information on DNA 5mC and 5hmC methylation status of proinflammatory genes in humans with PAH. (mergullo.net)
Macrophages1
- Mouse Disabled-2 (Dab2) was isolated as a 96 kDa phosphoprotein involved in CSF-1 signaling in macrophages, and was initially referred to as p96 [ 1 ]. (biomedcentral.com)
Transgenic mouse2
- By using transgenic mouse models and primary oligodendrocytes, we have found that SNARE-mediated exocytosis drives membrane addition in myelin sheaths. (stanford.edu)
- To this end, we generated a novel multicistronic BAC (bacterial artificial chromosome) transgenic mouse line under the regulatory elements of the Npas1 gene. (nih.gov)
Overexpression1
- Finally, we established that PCAF is required for regeneration following a conditioning lesion and PCAF overexpression promotes axonal regeneration similar to that of a conditioning lesion after CNS injury in spinal ascending sensory fibres. (nature.com)
Humans4
- Pulmonary exposure to multi-walled carbon nanotube s (MWCNTs) may cause fibrosing lesions in animal lungs, raising health concerns about such exposure in humans. (cdc.gov)
- To address these questions, we have produced high-titer antisera recognizing human SR-A by using mice deficient for SR-A (SR-A -/-). We show that SR-A -/- mice produce a significantly higher-titer immune response than do wild-type (SR-A +/+) littermates, with antisera of the former having a broad species reactivity and recognizing SR-A from humans, mice, and rabbits. (ox.ac.uk)
- Several mutations of the Tet-methylcytosine-dioxygenase-2 (gene coding for any DNA demethylase have been reported in humans with PAH. (mergullo.net)
- Mutations in the CLN8 gene, encoding an endoplasmic reticulum (ER) transmembrane protein of unknown function, underlie NCL phenotypes in humans and mice. (biomedcentral.com)
Pathways6
- A total of 318 differentially expressed genes were associated with anti-CD47 treatment, with enrichment for reduced signatures of extracellular matrix remodeling and upregluation of pathways involved in tertiary lymphoid structure formation. (sitcancer.org)
- Because the effect of haploinsufficiency for one protein is relatively small, we hypothesize that predisposition to cancer could be a result of the additive effect of heterozygosity for two or more genes, critical for pathways that control DNA damage signaling, repair or apoptosis. (aacrjournals.org)
- One of the factors that appears to have a role in many of these pathways is human RAD52, a DNA repair protein that was previously considered to be dispensable due to a lack of an observable phenotype in knock-out mice. (encyclopedia.pub)
- Given the cytotoxic nature of these lesions, the cell has developed multiple pathways of DSB repair, some which result in mutagenic repair, and others that use a template to ensure error-free repair. (encyclopedia.pub)
- 2018). While studying the tyrosyl-DNA-phosphodiesterase (TDP1 and TDP2) repair pathways for the excision of topoisomerases from DNA, Dr. Pommier revealed that TDP1 repairs a broad range of 3'-blocking lesions in addition to TOP1 (Murai et al. (cancer.gov)
- By comparing CCDKO mice with re-derived CCl2(-/-)/Crb1(Rd8/RD8), Cx3cr1(-/-)/Crb1(Rd8/RD8) and CCl2(-/-)/Cx3cr1(-/-)/Crb1(Rd8/RD8) mice, we observed a differential modulation of the retinal phenotype by genetic background and both chemokine signalling pathways. (ox.ac.uk)
Receptor5
- Adapting the Cre/loxP system that phage use to insert their genes into bacterial chromosomes, Tsien was able to shut down the activity of the NMDA receptor in the CA1 region of the hippocampus, an area critical for learning and memory. (the-scientist.com)
- Like patients who have lesions in this part of the brain, the mutant mice showed profound memory deficits, providing, says Tsien, "strong evidence that the NMDA receptor is indeed important for memory. (the-scientist.com)
- 18 Studies on B cell receptor gene usage of these infiltrating IgG positive lymphocytes demonstrate that they are of peripheral and not mucosal origin in UC versus controls. (bmj.com)
- Similar phenotypes are seen in schizophrenia-relevant genetic mouse models, such as synaptosomal associated protein of 25 kDa (Snap-25) point mutant mice, vasoactive intestinal peptide receptor 2 (Vipr2) knockout mice, and neuregulin 1 (Nrg1)-deficient mice. (ox.ac.uk)
- ZAP-70 deficiency is a rare autosomal recessive form of severe combined immunodeficiency (SCID) caused by mutations in the gene coding for T cell receptor z-chain associated protein kinase [ Chan et al. (lu.se)
Genetic4
- This model is amenable to additional genetic modifications and deletion of another cancer-related gene, Mmp9 , predictably alters the tumor microenvironment and facilitates cancer progression. (biologists.com)
- Rapid advances in gene editing and genetic technologies have revolutionised our ability to model human genetic disease and provided new hope for gene therapies. (biologists.com)
- 3, 5- 8 Animal models of inflammatory bowel disease (IBD), using knockout or transgenic mice, only acquire characteristic lesions when their colon is populated with normal commensal bacteria, 9- 15 while germ free mice with genetic susceptibility do not develop disease. (bmj.com)
- However, based on this view, the findings from patient studies and studies using genetic mouse models (Nrg1 deficiency) seem to be inconsistent with each other. (ox.ac.uk)
Mutations in the gene1
- X-linked agammaglobulinemia (XLA) is an immunodeficiency caused by mutations in the gene coding for Bruton's tyrosine kinase (BTK) [Väliaho et al. (lu.se)
Molecular2
- Joe has been one of the pioneers who has really pushed the power of mouse molecular genetics to get at the fundamental questions in learning and memory," says Rob Malenka of Stanford University. (the-scientist.com)
- Identification of a specific regulatory mechanism shared by several essential genes may lead to novel molecular strategies recapitulating the conditioning effect, thus non-surgically enhancing axonal regeneration in the CNS. (nature.com)
Protein4
- To facilitate purposeful investigation of the position of NADPH oxidase 1 ( NOX1 ) and related reactive oxygen species in most cancers cell signaling, we report herein the event and characterization of a novel mouse monoclonal antibody that particularly acknowledges the C-terminal area of the NOX1 protein. (aabioetica.org)
- In males, ACOT1 deficiency increased mitochondrial uncoupling protein-2 (UCP2) protein abundance while reducing 4-hydroxynonenal, a marker of oxidative stress, in white adipose tissue and liver of HFD-fed mice. (jci.org)
- The gene affected encodes a SH2D1A protein consisting only of a SH2 domain and a short C-terminal tail [Sayos et al. (lu.se)
- Noonan syndrome (NS) is caused by mutations in PTPN11 , a gene encoding the nonreceptor protein tyrosine phosphatase SHP2. (lu.se)
Deficient5
- Adult Dab2-deficient mice had a small but statistically significant increase in serum cholesterol levels. (biomedcentral.com)
- In later studies, RAD52 deficiency has been shown to be synthetically lethal with defects in BRCA genes, making RAD52 an attractive therapeutic target, particularly in the context of BRCA-deficient tumors. (encyclopedia.pub)
- More importantly, we used mice deficient in the gene encoding tryptophan hydroxylase 2 ( Tph2 ), which could not synthesize 5-HT in the brain. (aspetjournals.org)
- Electroencephalographic (EEG) recording also showed decreased sleep in Tph2 -deficient mice. (aspetjournals.org)
- Schizophrenia patients are impaired at recollection (and to a lesser extent, familiarity judgments), but Nrg1-deficient mice are impaired at familiarity-based object recognition, raising concerns regarding the validity of using these genetically modified mice to model recognition phenotypes observed in patients. (ox.ac.uk)
Retinal4
- The human phenotype is characterized by epilepsy, progressive psychomotor deterioration and visual loss, while motor neuron degeneration ( mnd ) mice with a Cln8 mutation show progressive motor neuron dysfunction and retinal degeneration. (biomedcentral.com)
- The major observation considered to be involved in the pathogenesis of retinal lesions is abnormalities in the internal limiting membrane formed by Müller cells, which is corresponding to the glia limitans formed by astrocytes in the brain. (intechopen.com)
- In order to understand phenotypic discrepancies in different chemokine knockout lines and to study how defects in Ccl2 and/or Cx3cr1 signalling contribute to the described early onset retinal degeneration, we defined primary and secondary pathological events in CCDKO mice. (ox.ac.uk)
- These findings indicate that CCDKO mice are not a model of AMD, but a model for an inherited retinal degeneration that is differentially modulated by Ccl2-Ccr2 and Cx3cl1-Cx3cr1 chemokine signalling. (ox.ac.uk)
Progression1
- A major conclusion from these data is that, contrary to one of the current views on tumorigenesis, inactivation of one allele of a tumor suppressor gene is enough to contribute to tumor progression. (aacrjournals.org)
Neuroscience1
- People in other fields had used the Cre/loxP system," says Ya-Ping Tang of the University of Chicago, Tsien's former postdoc, "but Joe Tsien was the first person to use it in neuroscience research, successfully producing a brain region-specific knockout. (the-scientist.com)
Axonal3
- Here we show through systematic epigenetic studies that the histone acetyltransferase p300/CBP-associated factor (PCAF) promotes acetylation of histone 3 Lys 9 at the promoters of established key regeneration-associated genes following a peripheral but not a central axonal injury. (nature.com)
- We examined both DNA methylation and various key histone modifications with regards to gene regulation following axonal injury. (nature.com)
- We found that p300/CBP-associated factor (PCAF)-dependent acetylation of histone 3 lysine 9 (H3K9ac), paralleled by a reduction in methylation of H3K9 (H3K9me2), occurred at the promoters of select genes only after PNS axonal injury. (nature.com)
Serum1
- examined, no important variations had been noticed in serum anti-OVA IgG ranges between wild-type mice (WT) and Nox1 -KO. (aabioetica.org)
Fetal1
- In this chapter, ocular lesions of fetal and child FCMD patients are presented, especially focusing on pathological findings of the retina, and functions of fukutin are discussed. (intechopen.com)
Mutation2
- Recently, our laboratory used linkage analysis to map the first disease-causing gene for CAD and MI to chromosome 15q26 in a large family and subsequently mutation analysis identified a 7-amio acid deletion in MEF2A, a gene encoding a transcription factor. (csuohio.edu)
- Furthermore, we provide evidence that a third autosomal recessive gene causes the degeneration in CCDKO mice and in all affected re-derived lines and subsequently demonstrated co-segregation of the naturally occurring RD8 mutation in the Crb1 gene. (ox.ac.uk)
Dependent4
- Using a mouse model of MWCNT exposure, we found that the tissue inhibitor of metalloproteinase 1 (Timp1) gene was rapidly and highly induced in the lungs by MWCNTs in a time- and dose-dependent manner. (cdc.gov)
- This transfer was dependent on the wraith of a betterer plasmid and doxepin buy doxepin without prescription hcl cream bioequivalence study database bells transfer genes. (upb.ro)
- Furthermore, we find that extracellular signal-regulated kinase (ERK)-mediated retrograde signalling is required for PCAF-dependent regenerative gene reprogramming. (nature.com)
- Homologous recombination (HR) is generally considered the most faithful DSB repair pathway, as it uses the intact sister chromatid as a donor, and it is highly dependent on breast cancer associated genes BRCA1 and BRCA2. (encyclopedia.pub)
Vivo3
- PV-knock-out mice manifest an impaired wound-healing response to in vivo injury, and a reduced scratch-wound reparation capacity in OHSCs. (unifr.ch)
- To further clarify the in vivo roles of Dab2 in development and physiology, we designed a new floxed allele to delete dab2 gene. (biomedcentral.com)
- Administration of AKB-9785 to Ang-1 knock out mice confirmed ligand-independent Tie-2 activation occurs in vivo . (ajkdblog.org)
Circadian2
- Sleep and circadian abnormalities and impaired recognition memory may be causally related in both schizophrenia patients and schizophrenia-relevant mouse models, since sleep deprivation, abnormal photic input, and the manipulation of core clock genes (cryptochrome 1/2) can all disrupt object recognition memory in rodent models. (ox.ac.uk)
- The recognition deficits observed in patients and mouse models (both schizophrenia-related and -unrelated) are discussed here in terms of the dual-process theory of recognition, which postulates that there are two recognition mechanisms-recollection versus familiarity-that can be selectively impaired by brain lesions, neuropsychiatric conditions, and putatively, sleep and circadian rhythm disruption. (ox.ac.uk)
Early-onset1
- In addition, patients with APP gene duplications or individuals with Down's syndrome (trisomy 21), who have increased dosage of the APP gene (located on chromosome 21), develop early-onset AD and overproduce Aβ 42 ( Hardy, 2006 ). (jneurosci.org)
Immune2
- We examine as but an unidentified position of NOX1 , a non-phagocytic isoform of the superoxide-generating NADPH oxidase, in immune responses utilizing Nox1 -knockout mice ( Nox1 -KO). (aabioetica.org)
- on immune cell gene silencing that offer selective targets for drugs altering DNA methylation. (mergullo.net)
Knock out2
- Whole-transcriptome analysis of ependymal-cell clusters in OHSCs revealed down-regulation of genes involved in cytoskeletal rearrangement, cell motility and cell adhesion in PV-knock out mice as compared with wild-type mice. (unifr.ch)
- Knock-out mice lacking the gene for the T-type calcium current in thalamic relay cells display a resistance to absence seizures (Kim et al. (scholarpedia.org)
Spinal1
- Interestingly, the lack of regeneration of injured ascending sensory fibres in the spinal cord can be partially enhanced by an injury to the peripheral branch (conditioning lesion) of DRG neurones 7 . (nature.com)
Peripheral blood1
- genes in peripheral blood of at-risk, new-onset and long-term type 1 DM , and healthy controls. (indexindex.com)
Chronic2
Stimulation1
- STAT5B acts as a part of the growth hormone signalling pathway leading to stimulation of insulin-like growth factor I (IGF-I) gene transcription [Woelfle et al. (lu.se)
Deficiency1
- 1997]. Mutations in the STAT1 gene cause complete STAT1 deficiency. (lu.se)
Phenotypes2
- Previously, two independent gene knockout studies have been reported, with some discrepancies in the observed embryonic phenotypes. (biomedcentral.com)
- Better understanding of new mutations and the wide range of possible phenotypes led to the development of a new nomenclature proposal, based on the gene and inheritance pattern. (medscape.com)
Significantly2
- The number of BrdU-positive (BrdU+) cells was significantly higher in the dentate gyrus of BACE1 -/- mice compared to both BACE1 +/- and BACE1 +/+ mice ( Fig. 1 ). (eneuro.org)
- BACE1 -/- mice exhibit significantly more BrdU+ cells compared to BACE1 +/+ or BACE1 +/- mice. (eneuro.org)
Alzheimer's1
- Although the etiology of Alzheimer's disease (AD) is not completely understood, the study of disease genes that cause AD has revealed important clues about the pathogenesis of this disorder. (jneurosci.org)
Proteins3
- Poly(ADP-ribosyl)ation is therefore an immediate, covalent, but transient post-translational modifications of nuclear proteins, induced by DNA lesions. (imim.es)
- To address this issue, primary mouse cells, haploinsufficient for one or two proteins, ATM and RAD9, related to the cellular response to DNA damage were examined. (aacrjournals.org)
- Most of these genes code for tumor suppressor proteins. (aacrjournals.org)
Model1
- Jessica Fowler and colleagues made a mouse model lacking the immunoglobulin recombination enzyme, RAG-2. (biologists.com)
Brain2
- For the next three-and-a-half years, in the early to mid-90s, Tsien labored over the development of a technique to produce a region-specific gene knockout in mouse brain. (the-scientist.com)
- Knockout of CD47 led to 60% increased survival and 89% decreased metastatic brain lesions in 4T1-bearing mice compared to controls. (sitcancer.org)
Cell2
- Cell survival was similar in BACE1 +/+, BACE1 +/-, and BACE1 -/- mice that received a single BrdU injection at two months of age and were killed four weeks later. (eneuro.org)
- Concentrating on DNA methylation equipment with oligonucleotide-based medications is an strategy examined in cell systems and with knockout mouse versions. (mergullo.net)
MRNA1
- Herein, we found that chromatin modifier MORC2 gained oncogenic potential in KIRC among the genes with APA reprogramming, and moreover, its oncogenic potential was enhanced by 3′UTR shortening through stabilization of MORC2 mRNA. (jci.org)
Mitochondrial1
- Several genes (SDHD, SDHB, SDHC) that belong to the mitochondrial complex II have been identified as involved in the so-called pheochromocytoma-paraganglioma syndrome. (medscape.com)
Development2
- Loss of function of oncogenes, tumor suppressor genes and DNA damage processing genes has been implicated in the development of many types of cancer, but for the vast majority of cases, there is no link to specific germ line mutations. (aacrjournals.org)
- Congenital Ang-1 knockout is fatal, as it is critically important for cardiovascular development. (ajkdblog.org)