RegenerationRegenerativeStem cellsBioengineeringCellsCell typesReviewMatureCharles GersbachExtracellular matrixBoneConnectiveAdipose tissueWovenSituChondrocytesDifferentiationDifferentiateMusculoskeletalTherapeuticImplantsAdditionallyHumanRepairClinicalDirectCreateStructureGoalTypeCreationJointStudyPotentialCulture
Regeneration1
- 2019) 3D printing approaches for cardiac tissue engineering and role of immune modulation in tissue regeneration. (aimspress.com)
Regenerative2
- In the last two decades, the ECFCs from various vascular disease patients have been widely used to study the diseases' pathophysiology ex vivo and develop cell-based therapeutic approaches, including vascular regenerative therapy, tissue engineering, and gene therapy. (bvsalud.org)
- In den letzten zwanzig Jahren wurden ECFCs von Patienten mit verschiedenen vaskulären Erkrankungen ex vivo verwendet, um die Pathophysiologie von Krankheiten zu studieren und Zell-basierte therapeutische Ansätze wie vaskuläre regenerative Therapie, tissue engineering, oder Gentherapie zu entwickeln. (bvsalud.org)
Stem cells4
- Differentiated airway epithelial cells can revert into stable and functional stem cells in vivo. (wikipedia.org)
- These early trials are showing roles for stem cells both in replacing damaged tissue as well as in providing extracellular factors that can promote endogenous cellular salvage and replenishment. (biomedcentral.com)
- The use of patient's own bone marrow aspirates, hematopoietic stem cells and MSCs, for heart muscle tissue repair can be puzzling because these cells do not normally contribute to the cardiac lineage types that are desired. (biomedcentral.com)
- 2010) Generation of transgene-free lung disease-specific human induced pluripotent stem cells using a single excisable lentiviral stem cell cassette. (aimspress.com)
Bioengineering1
- Manufacture of complex heart tissues: technological advancements and future directions[J]. AIMS Bioengineering, 2021, 8(1): 73-92. (aimspress.com)
Cells5
- After injury, mature terminally differentiated kidney cells dedifferentiate into more primordial versions of themselves and then differentiate into the cell types needing replacement in the damaged tissue Macrophages can self-renew by local proliferation of mature differentiated cells. (wikipedia.org)
- In newts, muscle tissue is regenerated from specialized muscle cells that dedifferentiate and forget the type of cell they had been. (wikipedia.org)
- The latest technological advances in stem cell biology and mechanical engineering provide new opportunities for cardiac tissue engineering, enabling the production of highly efficient differentiated cells and the manufacture of high-resolution complex cardiac tissues. (aimspress.com)
- The tissue originates from the proepicardial organ, with cells migrating to form the outer mesothelial layer, giving rise to n. (labroots.com)
- Therefore, we propose that damage to and subsequent release of mtDNA elicits a protective signalling response that enhances nDNA repair in cells and tissues, suggesting that mtDNA is a genotoxic stress sentinel. (regenerativemedicine.net)
Cell types1
- This includes bone and cartilage repair, cell types into which MSCs readily differentiate, and immune conditions such as graft versus host disease and autoimmune conditions that utilize the MSC's immune suppressive properties. (biomedcentral.com)
Review2
- In this review, we summarize the progress of stem cell technology in 3D bioprinting of heart tissue and the latest technological breakthroughs. (aimspress.com)
- The objective of this review is to explore the possibility of interdisciplinary research to solve the existing challenges in tissue engineering by summarizing the existing work and progress and pointing their current limitations. (aimspress.com)
Mature1
- Upon differentiation in culture, ECFCs are excellent surrogates for mature ECs showing the same phenotypic, genotypic, and functional features. (bvsalud.org)
Charles Gersbach1
- A team led by Drs. Farshid Guilak and Charles Gersbach at Duke University set out to create an artificial scaffold that could direct stem cells within to differentiate and form extracellular matrix. (nih.gov)
Extracellular matrix4
- The goal of this study was to generate a self-contained bioactive scaffold capable of mediating stem cell differentiation and formation of a cartilaginous extracellular matrix (ECM) using a lentivirus-based method. (duke.edu)
- This structure, called an extracellular matrix, allows cartilage to flex and absorb shock. (nih.gov)
- Without any extra prompting, the cells created a cartilage-like extracellular matrix within 4 weeks. (nih.gov)
- Osteoarthritis (OA) is a chronic joint disease characterized by articular cartilage degeneration, secondary bone hyperplasia, inadequate extracellular matrix synthesis and degeneration of articular cartilage. (bvsalud.org)
Bone3
- The approach could allow for the creation of orthopedic implants to replace cartilage, bone, and other tissues. (nih.gov)
- Collagens are fibrous proteins that serve as the building blocks of skin, tendon, bone, and other connective tissues. (nih.gov)
- MSCs can be sourced from a variety of tissues within the body, but bone marrow is the most frequently used starting material for clinical use. (biomedcentral.com)
Connective1
- The term "marrow stromal stem cell" was first used by Maureen Owen in 1988 because of their ability to self-renew (although this may be interpreted today as a high proliferative capacity) and the gene activation potential to differentiate into discrete connective tissue cells [ 3 ]. (biomedcentral.com)
Adipose tissue4
- Recently, adipose tissue, as a suitable source of harvesting mesenchymal stem cells, has attracted the attention of many researchers in the field of regenerative medicine. (bvsalud.org)
- Adipose tissue-derived mesenchymal stem cells can self-renew and differentiate into different types of cells such as adipocytes, chondrocytes, and osteoblasts. (bvsalud.org)
- Adipose tissue, especially brown type, is considered an attractive cell source for various therapeutic purposes, such as restoring damaged tissue or fighting against diseases such as obesity. (bvsalud.org)
- Recently, in the light of many efforts in the field of regenerative medicine, mice have gained increasing interest as a suitable source of adipose tissue for the extraction of mesenchymal stem cells, which can be used in the preclinical investigations in order to aid in the treatment of many human diseases. (bvsalud.org)
Woven2
- We then demonstrated that scaffold-mediated gene delivery of transforming growth factor β3 (TGF-β3), using a 3D woven poly(ε-caprolactone) scaffold, induced robust cartilaginous ECM formation by hMSCs. (duke.edu)
- The scientists coated a 3-D woven scaffold with a compound that can secure viruses to a surface but still allow them to transfer genes into target cells. (nih.gov)
Situ2
- The ability to induce and maintain differentiation of stem cells in situ could bypass these steps and enhance the success of engineering approaches for tissue regeneration. (duke.edu)
- This method opens new avenues in the development of bioactive implants that circumvent the need for ex vivo tissue generation by enabling the long-term goal of in situ tissue engineering. (duke.edu)
Chondrocytes2
- Chondrocytes, cells found throughout cartilage, produce and maintain the structure. (nih.gov)
- Cells within the artificial scaffold successfully differentiated into chondrocytes within 2 weeks. (nih.gov)
Differentiation2
- Current approaches require extensive cell manipulation ex vivo, using exogenous growth factors to drive tissue-specific differentiation, matrix accumulation, and mechanical properties, thus limiting their potential clinical utility. (duke.edu)
- Chondrogenesis induced by scaffold-mediated gene delivery was as effective as traditional differentiation protocols involving medium supplementation with TGF-β3, as assessed by gene expression, biochemical, and biomechanical analyses. (duke.edu)
Differentiate1
- After injury, mature terminally differentiated kidney cells dedifferentiate into more primordial versions of themselves and then differentiate into the cell types needing replacement in the damaged tissue Macrophages can self-renew by local proliferation of mature differentiated cells. (wikipedia.org)
Musculoskeletal2
- The ability to develop tissue constructs with matrix composition and biomechanical properties that promote rapid tissue repair or regeneration remains an enduring challenge in musculoskeletal engineering. (duke.edu)
- Creating replacements for musculoskeletal tissues is challenging. (nih.gov)
Therapeutic1
- These criteria have helped to drive standardization in the manufacturing and identity of MSC for therapeutic use, but does not capture whether MSCs derived and cultured from different tissues have equivalent therapeutic potential or potency. (biomedcentral.com)
Implants1
- This approach could allow for implants that restore function to a joint immediately and drive development of a mature, viable tissue replacement. (nih.gov)
Additionally2
- Additionally, there are still many technical and scientific obstacles that need to be addressed before 3D bioprinting can be used to create functional organs for transplants. (medgadget.com)
- Additionally, the expression of AKT1 was higher in GC tissues than in both paracancerous tissues and normal tissues, and patients resistant to chemotherapy expressed more AKT1 compared to those who were sensitive. (bvsalud.org)
Human1
- In Nov 2021, CollPlant launched a bioink named Collink.3D™ for use in the 3D bioprinting of human tissues, scaffolds, and organs. (medgadget.com)
Repair1
- Platelet-rich plasma (PRP) is a highly concentrated platelet-containing blood plasma that incorporates a significant amount of growth factors and cytokines needed to accelerate the tissue repair process. (bvsalud.org)
Clinical2
- We discuss the differential migratory, angiogenetic and immunomodulatory potential to understand the role that tissue source of MSC may play within a clinical context. (biomedcentral.com)
- In this review, we systematically summarize the advances in EV (especially exosomes) engineering for clinical application, focusing on strategies toward high yield, facile isolation, efficient cargo loading, improved delivery, and optimized manufacturing, which might unleash the infinite power of EVs in clinical translation. (thno.org)
Direct1
- PRP has been used effectively for many years in the treatment of various wounds by direct injection into the target tissue or impregnation with scaffold or graft materials. (bvsalud.org)
Create1
- With the rise of personalized medicine, bioprinting has the potential to create customized tissue for individual patients, which can help to improve outcomes and reduce the risk of rejection. (medgadget.com)
Structure1
- 3D printing allows for the precise control of the structure of the tissue, which is crucial for creating functional, living tissue structures. (medgadget.com)
Goal1
- Replacing cartilage in this and other situations has been a major goal in tissue engineering. (nih.gov)
Type1
- In osteoarthritis (the most common type of arthritis), cartilage breaks down and wears away. (nih.gov)
Creation1
- This technology has the potential to revolutionize the medical field by allowing for the creation of functional tissue for use in transplants, drug development, and other applications. (medgadget.com)
Joint1
- Cartilage is the slippery tissue that covers the ends of bones in a joint. (nih.gov)
Study1
- In this study, delivery vehicles comprised of microvesicles loaded with engineered minicircle (MC) DNA that encodes prodrug converting enzymes were developed as a cancer therapy in mammary carcinoma models. (regenerativemedicine.net)
Potential1
- 3D bioprinting offers a potential solution to this problem by creating functional, living tissue structures that can be used for transplants. (medgadget.com)
Culture1
- However, the variety of tissue sources, coupled with different isolation and culture protocols and the intrinsic variability of MSCs from donor to donor has led to wide variability regarding the description of MSC phenotypes and properties through the literature. (biomedcentral.com)