Lectins carbohydrate sequence. Medical search. Frequent questions

Lectins are a diverse group of proteins, both in terms of structure and activity. (embl.de)
When examined from the viewpoint of the bound sugar, the structural information accumulated so far on lectins and other proteins that are specific to galactose and glucose (or mannose), provides suggestive evidence for distinct ligand-dependent distribution of hydrogen-bond partners in the combining site. (embl.de)
Lectins are important adhesin proteins, which bind carbohydrate structures on host cell surface. (unl.edu)
After his stay at the USDA he spent three years at Cornell University at the Department of Food Science working with Prof. John Brady on a multitude of projects including: the simulation of carbohydrates interacting with each other and with proteins, either as substrates for enzymes, ligands for carbohydrate-binding proteins like lectins, or in general non-specific interactions, and the hydration of planar hydrophobic species. (bradley.edu)
On the basis of knockdown experiments at the larval stage the identification of PPLs in the shell matrix and CaCO3 crystallization analysis we conclude that two novel jacalin-related lectins participate in the biomineralization of nacre as matrix proteins. (cancer-ecosystem.com)
Lectins which are carbohydrate recognition proteins have been found in viruses bacteria fungi plants and animals. (cancer-ecosystem.com)
Changes in glycan structures and the interactions of these structures with endogenous carbohydrate- binding proteins, known as lectins, are now considered to be potential biomarkers on cancer cells for monitoring tu- mor progression. (fdocuments.net)
Lectins, a ubiquitous family of effector proteins, are capable of reversibly binding carbohydrate sequences with extraordinary selectivity, thus acting as translators of sugar-encoded signals. (ucm.es)
Galectin-3 belongs to the lectin family of carbohydrate binding proteins. (enquirebio.com)
The amino acid sequences of human and mouse Galectin-3 proteins share 80% homology. (enquirebio.com)
To determine whether the absence of this calcium\binding site affects the structure and function of human CD23, CD23 mutant proteins with increasingly murine\like sequences were generated. (treatmentforprostatecancer.info)
CD23 belongs to the C\type (calcium\dependent) lectin\like (CTLD) superfamily of proteins and is a trimer in its membrane\bound form. (treatmentforprostatecancer.info)
Proteins which contain carbohydrate groups attached covalently to the polypeptide chain. (lookformedical.com)
The chemical or biochemical addition of carbohydrate or glycosyl groups to other chemicals, especially peptides or proteins. (lookformedical.com)
Proteins that share the common characteristic of binding to carbohydrates . (lookformedical.com)
Some ANTIBODIES and carbohydrate-metabolizing proteins (ENZYMES) also bind to carbohydrates , however they are not considered lectins . (lookformedical.com)
PLANT LECTINS are carbohydrate-binding proteins that have been primarily identified by their hemagglutinating activity (HEMAGGLUTININS). (lookformedical.com)
Lectins are proteins of non-immune origin with at least one non-catalytic domain that reversibly binds to specific mono or oligo saccharides and are widely distributed in the plant kingdom. (ufc.br)
It belongs to a family of proteins called collectins, which consists of collagenous region and a carbohydrate recognition domain (Taylor et al. (springeropen.com)

Peanut agglutinin (PNA) is plant lectin protein derived from the fruits of Arachis hypogaea. (wikipedia.org)
For structural analysis of asparagine-linked carbohydrates, sugars are released from the protein backbone by enzymes such as PNGase F ( NEB #P0704 ). (neb.com)
We were able to identify several classes of antimicrobial peptides, such as buforins, peroniins and brevinins, as well as PLA 2 , lectins and galectins, combining protein sequencing and RNA-seq analysis for the first time. (biomedcentral.com)
C-type lectin (CTL) is a protein that binds to saccharides and plays an important role in parasite adhesion, host cell invasion and immune evasion. (bvsalud.org)
Many of the protein-protein interactions (PPI) or intra protein interactions are mediated by interactions between domains and specific patterns of sequence. (cnpem.br)
Specifically, we use computer simulations and formal theory to study problems in the field of carbohydrate and protein chemistry, focusing on structure/function relationships, polymer property prediction, and enzymatic biomass conversion. (bradley.edu)
Digestion of carbohydrate residues on the protein with an acetylglucosaminidase plus binding to several lectins indicates that the enzyme is glycosylated. (inra.fr)
A method was developed to enhance the carbohydrate homogeneity of recombinant protein. (utmb.edu)
The secreted protein was purified using a combination of ultrafiltration, lectin affinity chromatography, ion exchange chromatography and reverse-phase HPLC. (utmb.edu)
Subsequently, several groups identified new members of the galectin family in protein extracts obtained from various tissues by using their ability to bind galactose residues on rabbit erythrocytes and cloning approaches based on sequence homology. (fdocuments.net)
The protein moiety is the predominant group with the carbohydrate making up only a small percentage of the total weight. (lookformedical.com)
Conjugated protein-carbohydrate compounds including mucins , mucoid, and amyloid glycoproteins . (lookformedical.com)
Mannose binding lectin (MBL) is a liver derived protein which plays an important role in innate immunity. (springeropen.com)
Mannose binding lectin (MBL) is a liver derived acute phase protein. (springeropen.com)
A protein containing a carbohydrate group. (flashcardmachine.com)
Human mannose-binding protein carbohydrate recognition domain trimerizes through a triple α-helical coiled-coil. (lu.se)
C-reactive protein (CRP, not shown) leads to classic pathway activation analogous to lectin pathway activation by MBL and ficolins. (medscape.com)

High affinity sugar ligands of C-type lectin receptor langerin. (mpg.de)
Fragment-based design of novel lectin ligands. (mpg.de)
Their biological roles, initially understood as limited to recognition of endogenous ("self") carbohydrate ligands in embryogenesis and early development, dramatically expanded in later years by the discovery of their roles in tissue repair, cancer, adipogenesis, and regulation of immune homeostasis. (nsf.gov)
Due to a common structural element, the amino-terminal lectin-like domain, the selectins are able to bind to carbohydrate ligands. (biovendor.com)
Evidence suggesting that the interactions between lectins and their ligands have a major role in the different steps of cancer progression has accumulated at a rapid pace and has gained the attention of several on- cologists. (fdocuments.net)
All ligands showed different patterns of interaction with the residues of the carbohydrate binding site. (ufc.br)

Its extracellular region consists of two N-linked glycosylation sites (aa 73 and 159), a coil‑coil neck domain (aa 96‑136), a C‑type lectin-like domain (CTLD) of the type found in human DC‑SIGN and DC‑SIGN receptor (aa 165‑289), and a C-terminal Ca 2+ -dependent carbohydrate‑recognition domain (C-type CRD) (1). (rndsystems.com)
This domain, which is known as the C-type lectin domain (CTL) or as the carbohydrate-recognition domain (CRD), consists of about 110 to 130 residues. (embl.de)
PPL1 has two tandem carbohydrate recognition domain (CRD) structures in its sequence which has sequence homology with rhamnose-binding lectins (RBLs) from various fish eggs and a galactose-binding lectin (SUEL) from sea urchin eggs [24]. (cancer-ecosystem.com)
G3 is implicated in the intracellular processing of aggrecan and contains a C-type lectin carbohydrate recognition domain (CRD), frequent occurrences of a C-terminal short complement repeat (SCR) domain, and occasionally an N-terminal epidermal growth factor domain. (brighton.ac.uk)
Galectins are a family of ß-galactoside-binding lectins characterized by a unique sequence motif in the carbohydrate recognition domain, and evolutionary and structural conservation from fungi to invertebrates and vertebrates, including mammals. (nsf.gov)

For example in PNA-affinity chromatography the binding specificity of peanut agglutinin is used to isolate glycosylated molecules which have the sugar sequence Gal-β(1-3)-GalNAc. (wikipedia.org)
B cell superantigen activity through affinity for BCR carbohydrate is discussed. (harvard.edu)
CD62L binds to carbohydrates present on certain glycoforms of CD34, glycam-1, and MAdCAM-1 and with a low affinity to anionic oligosaccharide sequences related to sialylated Lewis X (sLex, CD15s) through its C-type lectin domain. (biolegend.com)
In 1994, a joint effort by several investigators in the field led to a new nomenclature in which the term "galectins" was used to define lectins with an affinity for - galactosides and with sequence similarity in the carbohydrate-binding site [3]. (fdocuments.net)
In this work, it was isolated lectin from Parkia panurensis seed (Ppal) specifies to mannose and glucose by combining the techniques of ammonium sulfate fractionation and affinity chromatography on a sepharose-mannose column. (ufc.br)
Lectin array technology is based on the lectin-glycan affinity ( 6 ), which is not suitable for glycoproteomic analysis. (frontiersin.org)

Abstract Galectins, highly conserved β-galactoside-binding lectins, have diverse regulatory roles in development and immune homeostasis and can mediate protective functions during microbial infection. (nsf.gov)
Introduction Galectins, formerly known as S-type lectins, rep- resent a family of evolutionarily conserved ani- mal lectins that are widely distributed from lower invertebrates to higher vertebrates. (fdocuments.net)
This β -sandwich fold is conserved in all galectins and includes a gly- cine, which stabilizes the galectin-carbohydrate interactions [4]. (fdocuments.net)

Available Structures of peanut agglutinin Because peanut agglutinin specifically binds a particular carbohydrate sequence it finds use in a range of methods for cell biology and biochemistry. (wikipedia.org)
The detection level accomplished in this study should help not only to further characterize anti-LOS antibodies in blood and body fluids but also to analyze carbohydrate structures that are recognized by them. (go.jp)
Through recognition of sugar structures by endogenous lectins, the discrimination between selfand non-self-glycosylation patterns lies behind the innate immunity response. (ucm.es)

The disulphide linkages and the glycoform distribution on all three glycosylation sites were characterized by combining N-terminal sequencing with matrix-assisted laser desorption ionization time of flight (MALDI-TOF) and ESI FT-ICR MS. The techniques confirmed the expected disulphide linkage pattern. (utmb.edu)
Using sugar-specific binding lectins, the UT-A3 glycosylation profile was examined. (emory.edu)
Mass spectrometry and lectin arrays are common technologies publicly adopted in glycosylation analysis. (frontiersin.org)

Mannose binding lectin gene 2 ( MBL2 ) polymorphisms are reported to be associated with various diseases. (springeropen.com)
It binds to carbohydrates on the surface of mannose-rich pathogens and mediates clearing by phagocytosis or complement activation (Nepomuceno et al. (springeropen.com)
The 45-kDa form was pulled down by lectin concanavalin A (Con A) and Galant husnivalis lectin (GNL), indicating an immature glycan with a high amount of mannose (Man), whereas the 65-kDa form is a mature glycan composed of acetylglucosamine (GlcNAc) and poly-N-acetyllactosame (poly-LacNAc) that was pulled down by wheat germ agglutinin (WGA) and tomato lectin, respectively. (emory.edu)

LSECtin (liver and lymph node sinusoidal endothelial cell C-type lectin), also known as C-type lectin superfamily 4, member G (CLEC4G), is a member of subgroup II of the C-type (Ca 2+ -dependent) lectin superfamily (1). (rndsystems.com)
Alignment of 131 sequences from all seven groups in the CRD superfamily defined a consensus length of 136 residues, in which 32% of residues were conserved. (brighton.ac.uk)
Although the G3 CRD sequences agreed with this consensus, they also contained five fully conserved basic residues that are atypical of the CRD superfamily. (brighton.ac.uk)
The alignment of 13 G3 SCR sequences with 101 sequences in the SCR superfamily showed good agreement with conserved residues in the SCR superfamily. (brighton.ac.uk)

May function in innate immunity through activation of the lectin complement pathway. (xenbase.org)
The complement system functions as an interactive sequence, with one reaction leading to another in the form of a cascade. (medscape.com)
Depending on the nature of complement activators, the classic pathway, the alternative pathway, or the more recently discovered lectin pathway is activated predominantly to produce C3 convertase. (medscape.com)

Using a new approach to reconstitute antigen-receptor interactions in a human reporter B cell line, we found that sequence-defined BCRs from the human germline repertoire could be triggered by both complementarity to influenza HA and a separate mode of signaling that relied on multivalent ligation of BCR sialyl-oligosaccharide. (harvard.edu)
Lectin-carbohydrate interactions: different folds, common recognition principles. (embl.de)
Homology modelling showed that four of these residues are located on a surface region on the CRD that is separate from the Ca2+-binding residues involved in carbohydrate interactions. (brighton.ac.uk)

The rat enzyme, however, is heavily glycosylated and binds to wheat-germ lectin-Sepharose 6MB and 5-hydroxytryptamine-agarose, but not to concanavalin A-Sepharose 4B. (wikigenes.org)

Incorporating monomers into sequence-defined synthetic macromolecules endows them to mimic nature which results in key residues being anchored in the molecular recognition pattern. (itu.edu.tr)
NH2-terminal sequencing of the abundant precursors confirmed that the intact subunit of concanavalin A was formed by the reannealing of two fragments, since the alignment of residues 1-118 and 119-237 was reversed in the final form of the lectin identified in the chase and the precursor first labeled. (rupress.org)

The mucin-like glycoprotein PSGL-1 (P-selectin Glycoprotein Ligand-1) has been cloned and sequenced. (biovendor.com)

Metabolic labeling of immature jackbean cotyledons with 14C-amino acids was used to determine the processing steps involved in the assembly of concanavalin A. Pulse-chase experiments and analyses of immunoprecipitated lectin forms indicated a complex series of events involving seven distinct species. (rupress.org)

There is also a chimeric form of galectin (i.e. galectin-3) that contains one CRD connected to a non-lectin domain. (fdocuments.net)
Human CD23 is usually a calcium\dependent (C\type) lectin\like domain name that has apparently lost its carbohydrate\binding capability. (treatmentforprostatecancer.info)

The name "peanut agglutinin" originates from its ability to stick together (agglutinate) cells, such as neuraminidase-treated erythrocytes, which have glycoproteins or glycolipids on their surface which include the Gal-β(1-3)-GalNAc carbohydrate sequence. (wikipedia.org)

The esterase was cleaved with two proteases, and the amino acid sequences from fourteen peptides were used to search GenBank. (inra.fr)

These observations spotlight the unique functions diversity and molecular evolution of this lectin family involved in the mollusk shell formation. (cancer-ecosystem.com)
Previously we isolated an 18 kDa lectin (which we now refer to as PPL1) from the mantle of large-winged pearl shells and determined its primary structure and molecular properties [23]. (cancer-ecosystem.com)
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. (lookformedical.com)

Previous studies showed that recombinant T. spiralis C-type lectin (rTsCTL) promotes larval invasion of intestinal epithelium cells (IEC), whereas anti-rTsCTL antibodies inhibits larval invasion. (bvsalud.org)

Developing controlled carbohydrate sequences has critical importance in understanding the multivalent binding motifs of oligosaccharide and sequence-controlled glycopolymers to various lectins. (itu.edu.tr)

1998 ). The MBL2 gene is composed of 7461 bases and lies between the regions 52765380 to 52772841 bp of chromosome no. 10 (NCBI reference sequence number NC_000010.11). (springeropen.com)

Druggability of mammalian C-type lectin receptors. (mpg.de)

Many of these domains function as calcium-dependent carbohydrate binding modules. (embl.de)

It is a member of the Lectin_legB PFAM family. (wikipedia.org)
Most members of this family appear to have signal sequences. (unl.edu)
The 15 members of the family are normally classified according to their struc- ture and number of carbohydrate recognition domains (CRDs). (fdocuments.net)

Glycobiology is the study of the structure, function and biology of carbohydrates, also called glycans, which are widely distributed in nature. (neb.com)

Binds to GalNAc and GlcNAc carbohydrate moieties (PubMed:12679857). (xenbase.org)

Putative alpha-1,2-mannosidase [Carbohydrate transport and metabolism]. (unl.edu)

Studies to determine the three-dimensional structure of Parkia biglobosa seed lectin (PBL) showed the complete crystal structure of PBL complexed withα-methyl-manosídeo consists of a monomer in the asymmetric unit consists of threeβ-prism domains with homologous amino acid sequences arranged in tandem. (ufc.br)
The structure of the monomer has three carbohydrate binding sites, each binding site to carbohydrates is located in one of theβ-prism domains. (ufc.br)

Consideration of the tertiary structure of the glycosylated precursor and mature lectin showed that the entire series of processing events could occur without significant refolding of the initial translational product. (rupress.org)

Carbohydrate microarray analyses, corroborated by structural considerations, show that TgMIC13, TgMIC1, and its homologue Neospora caninum MIC1 (NcMIC1) share a preference for α2-3- over α2-6-linked sialyl-N-acetyllactosamine sequences. (unl.pt)

To overcome the host plant defense response, M. phaseolina encodes a significant number of P450s, MFS type membrane transporters, glycosidases, transposases, and secondary metabolites in comparison to all sequenced ascomycete species. (biomedcentral.com)
Many plant lectins change the physiology of the membrane of BLOOD CELLS to cause agglutination, mitosis, or other biochemical changes. (lookformedical.com)

The results obtained here indicate that the binding of IgG in NHS to the LOS and LPS samples is probably due to their carbohydrate moieties. (go.jp)

Although the use of lectins as tools for glycotypification of tissues in physiology, or of bacterial strains in microbiology, was long established, lectin-based high-scale analytical methodologies for classification of EV are still missing. (ucm.es)

Furthermore it was found that trehalose which is usually specific recognizing carbohydrates for PPL2A and is abundant in the secreted fluid of mantle functions as a regulatory factor for biomineralization via PPL2A. (cancer-ecosystem.com)

Lectins are responsible for cell surface sugar recognition in bacteria, animals, and plants. (embl.de)
The carbohydrate specificity of diverse lectins to a large extent dictates bacteria tissue tropism by mediating specific attachment to unique host sites expressing the corresponding carbohydrate receptor. (unl.edu)

The CRD and SCR domains in 13 G3 sequences were each subjected to structural analysis. (brighton.ac.uk)

However, a variety of lectins occur in animal species where they serve diverse array of functions through specific carbohydrate recognition. (lookformedical.com)

Dr. Schnupf's primary teaching responsibility is the General Chemistry and Physical Chemistry sequence. (bradley.edu)

However, the three lectins also display differences in binding preferences. (unl.pt)

The resulting sequences were submitted to functional annotation against non-redundant NCBI database and Database of Anuran Defense Peptide. (biomedcentral.com)

Brady, J. "Comparison of the simulations of cellulosic crystals with three carbohydrate force fields", Carbohydrate Research 2016 , 422, 17-23. (bradley.edu)

Computational and experimental prediction of human C-type lectin receptor druggability. (mpg.de)
Human LSECtin shares 64% aa sequence identity with mouse LSECtin. (rndsystems.com)
The calcium\binding site classically required for carbohydrate binding in C\type lectins is usually absent in human CD23 but is present in the murine molecule. (treatmentforprostatecancer.info)

The sequence starts with the binding of 2 or more C1q recognition units to the Fc nonantigen binding part of IgG and IgM molecules. (medscape.com)

These lectins are presented as valuable tools for biotechnological byplay important biological functions such as recognition of cancer cells, mitogenic agents, immune suppressants among others. (ufc.br)

All of the carbohydrate chains are N-linked. (wikigenes.org)

Fragment-screening of N-acetylmannosamine kinase reveals non-carbohydrate inhibitors. (mpg.de)

Carbohydrates are traditionally considered to be an important source of energy for living organisms. (fdocuments.net)
Only the non-synonymous SNPs (nsSNPs), also called as missense variants are particularly important as they result to changes in the translated amino acid residue sequence. (springeropen.com)

Activation of immune cells (but not B cells) with lectins is widely known. (harvard.edu)

A strikingly distinct set of carbohydrate esterases (CE) are present in M. phaseolina , with the CE9 and CE10 families remarkably higher than any other fungi. (biomedcentral.com)

Anatomy1

Organisms5

Chemicals and Drugs27

Phenomena and Processes7

Technology, Industry, Agriculture2

Information Science3

Health Care1

Proteins19

Protein17

Ligands6

Recognition domain5

Affinity6

Galectins3

Structures3

Glycosylation3

Mannose3

Superfamily4

Complement3

Interactions3

Wheat germ1

Residues2

Glycoprotein1

Concanavalin1

Domain2

Glycoproteins1

Peptides1

Molecular3

Larval1

Oligosaccharide1

MBL21

Receptors1

Calcium-dependent1

Family3

Widely distributed1

GlcNAc1

Putative1

Monomer2

Precursor1

Analyses1

Plant2

Moieties1

Physiology1

Abundant1

Bacteria2

Domains1

Species1

Chemistry1

Differences1

Annotation1

20161

Human3

Molecules1

Functions1

Chains1

Reveals1

Important2

Immune1

Fungi1