Laser microscopy confocal. Medical search. Frequent questions

Confocal laser scanning microscopy (CLSM) has rapidly become an essential tool in the life sciences laboratory, enabling high-resolution and minimally intrusive optical sectioning of fluorescent samples. (strath.ac.uk)
Confocal laser scanning microscopy is mainly used in biomedical sciences for high-resolution analysis of cell and tissue structures labeled with fluorescent markers. (lu.se)
1 - Blind Deconvolution for Confocal Laser Scanning Microscopy . (inria.fr)
Confocal laser scanning microscopy is a powerful technique for studying biological specimens in three dimensions (3D) by optical sectioning. (inria.fr)
Confocal microscopy, most frequently confocal laser scanning microscopy (CLSM) or laser scanning confocal microscopy (LSCM), is an optical imaging technique for increasing optical resolution and contrast of a micrograph by means of using a spatial pinhole to block out-of-focus light in image formation. (wikipedia.org)
Dedicated confocal laser scanning microscopy laboratory comprising Leica and Zeiss systems with separate preparation area. (warwick.ac.uk)
The ink penetration and distribution were characterized with Confocal Laser Scanning Microscopy (CLSM), whereas the microstructure of the paper surface was observed using Atomic Force Microscopy (AFM). (ncsu.edu)
Confocal laser scanning microscopy evaluation of roots subjected to activation protocol in endodontic microsurgery. (bvsalud.org)
After 7 days, the roots were cut at 1mm from the apex and the slices were evaluated with confocal laser scanning microscopy . (bvsalud.org)
Immunofluorescence-based confocal laser scanning microscopy, semithin resin section morphology and electron microscopy were utilized. (nih.gov)
Confocal laser scanning microscopy was used to image and quantify surfactant remaining on particles internalized by cells. (cdc.gov)
In our laboratories, you can use both standard microscope techniques, like scanning electron microscopy (SEM), transmission electron microscopy (TEM) and confocal laser scanning microscopy (CLSM), as well as infra-red based analysis techniques and micro-computed tomography (micro-CT). (lu.se)
For this purpose we used an ensemble of complementary structural techniques, including Neutron Reflectometry, Grazing Incidence Small-Angle Neutron Scattering, on a nanometer lengthscale and Confocal Laser Scanning Microscopy on a micrometer length scale. (lu.se)
In addition, Confocal Laser Scanning Microscopy analyses highlighted that NPs interact with cubic and lamellar phases according to two distinct mechanisms, related to the different structures of the lipid assemblies. (lu.se)
An important criterion is that the particle size is large enough to admit structural analyses via confocal laser scanning microscopy (CLSM). (lu.se)
Vesicle fusion was also studied with giant uni-lamellar vesicles using rapid confocal laser scanning microscopy. (lu.se)
A short introduction and hands-on training of optical and electron microscopy as well as fluorescence and confocal laser scanning microscopy is included. (lu.se)

The anterior cornea of four human volunteers was examined by an in-house developed confocal laser scanning microscope based on a commercial available instrument (Heidelberg Retina Tomograph II, Heidelberg Engineering GmbH, Germany). (arvojournals.org)

We report the realization of a confocal THz microscope based on a ∼ 3 THz quantum cascade laser source. (lancs.ac.uk)
Light travels through the sample under a conventional microscope as far into the specimen as it can penetrate, while a confocal microscope only focuses a smaller beam of light at one narrow depth level at a time. (wikipedia.org)
In contrast, a confocal microscope uses point illumination (see Point Spread Function) and a pinhole in an optically conjugate plane in front of the detector to eliminate out-of-focus signal - the name "confocal" stems from this configuration. (wikipedia.org)
Instruction in the confocal and multiphoton microscope with the aim of working independently. (uni-ulm.de)
Instruction in the HTS confocal microscope for long-term live cell observations. (uni-ulm.de)
Laser scanning cytometry (LSC) generates quantitative information on immune receptor expression from cells cytocentrifuged onto a microscope slide. (ersjournals.com)
Laser scanning cytometry (LSC) is a new technique that potentially fulfils these requirements by combining the benefits of FC with those of IA and is built around the epi-fluorescence microscope 10 . (ersjournals.com)
To calculate the proportion of dead E. faecalis cell volume, stained using LIVE/DEAD BacLight Bacterial Viability Kit and were scanned using confocal laser scanning microscope (CLSM). (comu.edu.tr)
Even though resolution is somewhat enhanced with confocal microscopy over conventional widefield techniques, it is still considerably less than that of the transmission electron microscope. (olympusconfocal.com)
When imaged with a laser scanning confocal microscope (Figure 1(d)), the medulla thick section reveals a significant degree of structural detail. (olympusconfocal.com)
Following Minsky's work, M. David Egger and Mojmir Petran fabricated a multiple-beam confocal microscope in the late 1960s that utilized a spinning (Nipkow) disk for examining unstained brain sections and ganglion cells. (olympusconfocal.com)
Continuing in this arena, Egger went on to develop the first mechanically scanned confocal laser microscope, and published the first recognizable images of cells in 1973. (olympusconfocal.com)
Fortuitously, shortly after Minsky's patent had expired, practical laser scanning confocal microscope designs were translated into working instruments by several investigators. (olympusconfocal.com)
Dutch physicist G. Fred Brakenhoff developed a scanning confocal microscope in 1979, while almost simultaneously, Colin Sheppard contributed to the technique with a theory of image formation. (olympusconfocal.com)
Electrochemical scanning microscope mounted on a confocal microscope . (warwick.ac.uk)
The most widely used three-dimensional imaging technology is the scanning confocal laser microscope. (photonics.com)
We have a Nikon A1R MP Confocal Microscope. (unifr.ch)
Motorized confocal laser scanning microscope equipped with both a high-resolution galvano scanner and a high-speed (15 fps) 1024 x 1024 resonant scanner capable of capturing high-quality confocal images of tissues, cells and molecular events at high speed and enhanced sensitivity. (lu.se)
Motorized confocal laser scanning microscope equipped with a high-resolution galvano scanner capable of capturing high-quality confocal images of tissues, cells and molecular events at enhanced sensitivity. (lu.se)
Confocal Microscope Platform at MultiParkLeica SP8 confocal microscope for imaging of fixed tissue and cells. (lu.se)
This inverted confocal microscope includes a motorized stage and a two channel confocal scanning head with spectral detection. (lu.se)
Facilities for 3D optical imaging and electron microscopy at Materials Engineering with instruments including E-SEM FEI Quanta 200 MKII with analytical system Pegasus (EBSD+EDS) from EDAX and optical microscope Keyence VHX-6000. (lu.se)
The Leica SP8 laser scanning confocal microscope system in A10 has been up and running since late 2019. (lu.se)
The Zeiss 780 confocal laser-scanning inverted microscope allows for high-resolution imaging of fixed unstained and stained fluorescent samples and time-lapse imaging of live-cells. (lu.se)

Most commercially available CLSM systems employ a gas laser, e.g. a Kr/Ar laser, to provide the excitation radiation. (strath.ac.uk)

Correlative light and electron microscopy (CLEM) is a powerful method to investigate the structure-function relationship in biology. (zeiss.com)
This may be performed using either light or electron microscopy, as discussed below. (cdc.gov)
If samples are grossly contaminated by nonasbestiform fibers, then transmission electron microscopy (NIOSH Method 7402) should be used for positive identification. (cdc.gov)

The principle of confocal imaging was patented in 1957 by Marvin Minsky and aims to overcome some limitations of traditional wide-field fluorescence microscopes. (wikipedia.org)
Confocal microscopes work on the principle of point excitation in the specimen (diffraction limited spot) and point detection of the resulting fluorescent signal. (wikipedia.org)
Four types of confocal microscopes are commercially available: Confocal laser scanning microscopes use multiple mirrors (typically 2 or 3 scanning linearly along the x- and the y- axes) to scan the laser across the sample and "descan" the image across a fixed pinhole and detector. (wikipedia.org)
Spinning-disk (Nipkow disk) confocal microscopes use a series of moving pinholes on a disc to scan spots of light. (wikipedia.org)
Laser scanning microscopes with highly sensitive detectors and ultra-short pulsed femtosecond laser technology are available, as well as inverted microscopes for confocal long-term imaging. (uni-ulm.de)
The Technology Platform Light Microscopy (TPLM) operates advanced light microscopes and image analysis resources and develops new technologies for light microscopy since 2022. (uni-hamburg.de)
The TPLM provides access to light microscopes at the Centre for Structural Systems Biology (CSSB) in the Advanced Light and Fluorescence Microscopy (ALFM) Core Facility and at the Department of Biology with the Light Microscopy Platform at the Institute of Plant Science and Microbiology (IPM) and the Institute of Cell and Systems Biology of Animals (IZS). (uni-hamburg.de)

There has been a tremendous explosion in the popularity of confocal microscopy in recent years, due in part to the relative ease with which extremely high-quality images can be obtained from specimens prepared for conventional fluorescence microscopy, and the growing number of applications in cell biology that rely on imaging both fixed and living cells and tissues. (olympusconfocal.com)
Presented in Figure 1 are a series of images that compare selected viewfields in traditional widefield and laser scanning confocal fluorescence microscopy. (olympusconfocal.com)
Wide-field fluorescence microscopy is an extensively used microscopy technique that illuminates the entire objective field of view (FOV) with excitation light and collects the resulting fluorescence signal. (lu.se)

A non-comprenhsive list of resources for microscopy at Lund University. (lu.se)

It has been demonstrated that with the use of a minimal number of optical components, high quality confocal images can be obtained from laser diodes operating around 406nm. (strath.ac.uk)

Tony Wilson, Brad Amos, and John White nurtured the concept and later (during the late 1980s) demonstrated the utility of confocal imaging in the examination of fluorescent biological specimens. (olympusconfocal.com)
Photobleaching and low penetration depth, caused by the high scattering of the visible light, limit the utility of confocal microscopy in certain applications, particularly with living cells and/or thicker tissue samples. (photonics.com)

Confocal microscopy provides the capacity for direct, noninvasive, serial optical sectioning of intact, thick, living specimens with a minimum of sample preparation as well as a marginal improvement in lateral resolution compared to wide-field microscopy. (wikipedia.org)

abstract = "We report on the application of a novel blue laser diode source to confocal microscopy. (strath.ac.uk)

Posteriormente los dientes fueron cortados transversalmente y se realizaron fotomicro- grafías del tercio cervical, medio y apical a través de la técnica de microscopia confocal de barrido por láser. (bvsalud.org)

The Core Facility "Confocal and Multiphoton Microscopy" is a member of the Center for Translational Imaging MoMAN . (uni-ulm.de)
The Core Facility "Confocal and Multiphoton Microscopy" of the Faculty of Medicine has existed in its current size since spring 2013. (uni-ulm.de)
The facility provides expertise in the field of confocal and multiphoton microscopy, data analysis, evaluation and interpretation as well as the conception and implementation of experiments. (uni-ulm.de)
The Core Facility "Confocal and Multiphoton Microscopy" offers services and guidance for all scientific questions concerning live cell microscopy. (uni-ulm.de)
In vivo vMSOT brain imaging of P301L mice showed higher retention of PBB5 in the tau-laden cortex and hippocampus compared to wild-type mice, as confirmed by ex vivo vMSOT, epi-fluorescence, multiphoton microscopy, and immunofluorescence staining. (springer.com)
Applications also include cell sorting, multiphoton imaging, matrix-assisted laser desorption ionization time-of-flight (Maldi-Tof) for protein analysis, fluorescence analysis in proteomics and genomics, and a novel treatment for ischemic strokes. (photonics.com)
These limitations are virtually eliminated in multiphoton imaging, a technique that relies on the fact that an ultrafast (femtosecond or picosecond) laser of modest average power (up to 2 W) can deliver peak powers of several tens of kilowatts. (photonics.com)
Because the amount of laser energy that is actually absorbed is relatively low, multiphoton imaging is much more compatible with live-cell studies than is scanning confocal laser microscopy. (photonics.com)
Multiphoton imaging is a large market for ultrafast lasers and was a major factor leading to the development of fully automated "one box" wavelength-tunable ultrafast lasers (Figure 1). (photonics.com)
A multiphoton excited image such as this bovine pulmonary endothelial cell relies on an ultrafast laser. (photonics.com)
One of the newest techniques combines fluorescence resonance energy transfer, or FRET, with either confocal or multiphoton imaging. (photonics.com)

The combined use of fluorescent in situ hybridization with confocal scanning laser microscopy demonstrated that anaerobic granule topography depends on granule origin and feeding. (canada.ca)
The Imaging Core Facility offers microscopy systems that enable advanced imaging of fluorescent and non-fluorescent live cells and fixed-stained biological specimens. (lu.se)

Likewise, widefield fluorescence imaging of whole rabbit muscle fibers stained with fluorescein produce blurred images (Figure 1(b)) lacking in detail, while the same specimen field (Figure 1(e)) reveals a highly striated topography in confocal microscopy. (olympusconfocal.com)

It is a facility specialised in molecular imaging that provides a unique spectrum of optical microscopy and is open to all scientists from the Faculty and University. (uni-ulm.de)
Confocal microscopy offers several advantages over conventional widefield optical microscopy, including the ability to control depth of field, elimination or reduction of background information away from the focal plane (that leads to image degradation), and the capability to collect serial optical sections from thick specimens. (olympusconfocal.com)
In fact, confocal technology is proving to be one of the most important advances ever achieved in optical microscopy. (olympusconfocal.com)

To date, where AM numbers are limited or if AM morphology is required, image analysis (IA) systems such as confocal microscopy have been used 8 . (ersjournals.com)

Single-mode and multi-mode laser sources for photoactivation and imaging using widefield, confocal, TIRF and other modalities. (cairn-research.co.uk)
To compliment our LED illuminators we offer a range of laser-based solutions for Confocal and Widefield microscopy, TIRF, FRAP, LightSheet, Photolysis and OptoGenetics. (cairn-research.co.uk)

It's equipped with four different lasers with excitation wavelengths of 405, 488, 561 and 640 nm. (unifr.ch)
Capable of two-color STED microscopy with lateral resolution ~50nm with excitation wavelengths 561nm and 640nm. (lu.se)

The basic concept of confocal microscopy was originally developed by Marvin Minsky in the mid-1950s (patented in 1957) when he was a postdoctoral student at Harvard University. (olympusconfocal.com)

This technique also delivers an inherently high signal-to-noise ratio because the laser and detection wavelengths are widely separated. (photonics.com)

Participants underwent imaging with confocal scanning laser tomography for measurement of DM rim area (DM-RA) and with spectral domain optical coherence tomography (SD OCT) for quantification of a BMO-based neuroretinal rim parameter, minimum rim width (BMO-MRW), and RNFL thickness. (nih.gov)

ZEISS Celldiscoverer 7 is an automated microscopy platform designed for live cell imaging and analysis. (lu.se)
The Zeiss 780 is equipped with six laser lines 405 nm, 458/488/514 nm, 561 nm and 633 nm and 10x, 20x, 40x water, 40x oil, 63x water, 63x oil objectives. (lu.se)

We also support researchers in image processing and quantitative analysis of light microscopy data. (uni-hamburg.de)
Cellomics Platform at MultiParkThe Cellomics ArrayScan VTI HCS Reader enables automated acquisition, visualization and quantification of a large number of images, with an automated fluorescence and brightfield microscopy allowing extraction of quantitative data from intensity and area measurement in high-throughput format. (lu.se)

Cells are first immobilised on a conventional glass slide by centrifugation or adherence, and after immunostaining are scanned sequentially by two lasers (argon and helium-neon, emitting at 488 and 633 nm, respectively). (ersjournals.com)
These techniques employ a wide variety of laser technologies, from humble helium-neon and air-cooled ion lasers to cutting-edge solid-state and ultrafast lasers. (photonics.com)

Laser diversity is key to widespread penetration in the fast-growing biomedical market. (photonics.com)
The laser is now well-established as a critically enabling tool in biomedical applications that range from research and clinical work at the molecular level to direct human applications for medical conditions such as strokes. (photonics.com)
The CIC serves the light microscopy needs of Drexel researchers from a variety of academic colleges and departments including the College of Arts and Sciences, the College of Engineering, the School of Biomedical Engineering, and the College of Medicine. (drexel.edu)

The choice for this instrument is the air-cooled argon-ion laser, which delivers a few tens of milliwatts of multiline output power in the blue/green wavelength region between 457 and 514 nm, or the air-cooled argon/krypton mixed-gas ion laser, which delivers additional output at 568 nm (yellow). (photonics.com)

Three-dimensional microscopy provides a powerful method for probing cellular structure, physiology and biochemistry. (photonics.com)

Infrastructure: The TPLM provides access to state-of-the-art light microscopy infrastructure and image analysis resources and regularly renews and expands its equipment. (uni-hamburg.de)

The Fandango 515 nm solid-state laser from Cobolt AB offers up to 150 mW CW output power, making it ideal for fluorescence applications such as confocal microscopy and single molecule analysis. (laserfocusworld.com)
Cobolt AB, Swedish manufacturer of low-noise DPSS lasers, announces the release of a higher power model of its unique 515 nm solid-state laser. (laserfocusworld.com)
The Cobolt Fandango™, now available with up to 150 mW CW output power from the same small package, is not only perfectly suited for power demanding fluorescence analysis applications such as confocal microscopy, high-speed plate reading and single molecule analysis, but now with even higher output power, as a general all solid state alternative to bulky Ar+ gas lasers. (laserfocusworld.com)
The Cobolt Fandango™ is a continuous-wave solid-state laser operating at a fixed wavelength 515 nm and with output powers of 25 mW, 50 mW, 100 mW and now 150 mW. (laserfocusworld.com)
Cobolt supplies compact and efficient diode-pumped solid-state lasers in the visible and near IR regions, for stand-alone use or OEM integration in equipment for bio-analysis, imaging, range finding and displays. (laserfocusworld.com)
Cobolt lasers are built on proprietary PPKTP technology in a patent pending manufacturing technique of HTCureTM technology and combine top-class wall-plug efficiency with low noise and compact size. (laserfocusworld.com)

The Leica Stellaris 5 is equipped with a White Light Laser (WLL) as excitation light source which convert s monochromatic laser light into a rainbow of spatially coherent light covering a large p art of the visible spectrum. (lu.se)

The instrument employs the spectral detector GaAsp that allows for high sensitivity detection of 32-channels and three additional detectors (PMTs), two for confocal detection and one for transmitted light detection. (lu.se)

Air-cooled ion lasers provide this flexibility with high reliability, the requisite TEM00 beam output and low cost of ownership. (photonics.com)

In this project, new illumination schemes for light cryo-microscopy (cryoLM) are developed. (uni-hamburg.de)

Method Development: The TPLM develops new methods and technologies for light microscopy that are of use for researchers on campus. (uni-hamburg.de)
We offer the courses Common Microscopy Methods in Biological Research (external website) and Microscopy - Bioimaging (external website) for PhD and Master students. (lu.se)
The course gives an overview and hands-on training on common low- and high-resolution microscopy methods. (lu.se)
Case-studies highlight how microscopy methods can be applied in research. (lu.se)

Aim To provide a new tool for the evaluation of altered ocular surfaces by using a combination of impression cytology, laser scanning confocal microscopy and advanced image analysis. (bmj.com)
Evaluation of dentin permeability after the use of different irrigation protocols and instrumentation techniques by histochemical analysis using digital microscopy and confocal laser microscopy. (usp.br)
We thank the Technology Platform Light Microscopy (TPLM) at Universität Hamburg (UHH), in particular XXX, for support with light microscopy image acquisition and analysis. (uni-hamburg.de)
At our microscopy platform in Lund, you meet personnel with expertise and long experience working with biological material and microscopy, imaging, and analysis. (lu.se)

In contrast, a thin optical section of the same grain (Figure 1(f)) acquired with confocal techniques displays a dramatic difference between the particle core and the surrounding envelope. (olympusconfocal.com)

The source has the potential to be a replacement for argon lasers in a range of fluorescence based imaging systems. (strath.ac.uk)
This technique is obviously time consuming when one deal with high dimensional data such as in 3D microscopy imaging. (inria.fr)

The mission of the Technology Platform Light Microscopy (TPLM) is to enable researchers to use the full spectrum of advanced light microscopy technologies for their research projects. (uni-hamburg.de)

High resolution is achieved by scanning the sample with a finely focussed laser beam, and exclusion of out-of-focus fluorescence by a confocal aperture. (lu.se)
Fluorescence induced in the beam waist is imaged in a confocal arrangement onto an aperture before reaching a low-noise detector. (photonics.com)
This aperture serves to block any light that does not originate from the focus of the laser. (photonics.com)

The basic key to the confocal approach is the use of spatial filtering techniques to eliminate out-of-focus light or glare in specimens whose thickness exceeds the immediate plane of focus. (olympusconfocal.com)
In FRET, one chemical species absorbs the laser light. (photonics.com)
Teaching: The TPLM offers courses on the basics of light microscopy and advanced light microscopy techniques. (uni-hamburg.de)
Joint development and implementation of projects in the field of light microscopy. (uni-hamburg.de)
The technology Platform Light Microscopy team supports you in planning and performing light microscopy experiments and in analyzing light microscopy image data. (uni-hamburg.de)
The TPLM is open to collaborative projects with a particular focus on method development for light microscopy. (uni-hamburg.de)
If you are using equipment or support from the Technology Platform Light Microscopy (TPLM), we ask you to indicate this in your publication. (uni-hamburg.de)
1 µm in diameter, then polarized light microscopy (NIOSH Method 7403) may be useful in identifying polymorphs (NIOSH 1987). (cdc.gov)

A resolution as low as 67 μm is achieved, with a large contrast enhancement allowed by the confocal geometry. (lancs.ac.uk)

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