Lactis saccharomyces cerevisiae proteins. Medical search. Frequent questions

Here we report isolation of Kluyveromyces lactis homologues of these genes. (vtt.fi)
The encoded Kluyveromyces proteins are up to 70% identical with the S. cerevisiae homologues at the amino acid level and can functionally replace them. (vtt.fi)
it is thermo-tolerant and can assimilate nitrate (see also Kluyveromyces lactis). (wikipedia.org)
Kluyveromyces lactis is a yeast regularly used for the production of kefir. (wikipedia.org)
To explore the role of STE12 in the pheromone induction of alpha-specific genes, we cloned STE12 and MAT alpha 1 homologs from the related yeast Kluyveromyces lactis. (embl-heidelberg.de)
Carbon source-dependent transcriptional regulation of the QCR8 gene in Kluyveromyces lactis. (ox.ac.uk)
2016). Production of orotic acid by a Klura3δ mutant of Kluyveromyces lactis . (up.pt)
In this work, we aimed to study and compare Trk proteins from Debaryomyces hansenii, Hortaea werneckii, Kluyveromyces marxianus, and Yarrowia lipolytica, four biotechnologically relevant yeasts that tolerate various extreme environments. (bvsalud.org)
Mitochondrial distribution and morphology protein 12 OS=Kluyveromyces lactis (strain ATCC 8585 / CBS. (yeastrc.org)
Lactose regulatory protein LAC9 OS=Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / N. (yeastrc.org)
A) Saccharomyces cerevisiae , B) Kluyveromyces waltii , C) Zygosaccharomyces rouxii . (biomedcentral.com)
The genes will be expressed in different yeasts (Saccharomyces cerevisiae, Pichia pastoris, Hansenula polymorpha, Kluyveromyces lactis) to identify the most suitable host for heterologous expression. (lu.lv)
We will also discuss potential approaches to humanize yeast in order to create modification patterns resembling the situation in mammalian cells, which could enhance the value of Saccharomyces cerevisiae and Kluyveromyces lactis as disease models. (microbialcell.com)

Saccharomyces cerevisiae S288C Cox15p (COX15), partial mRNA. (genscript.com)

The first yeast expression platform was based on the baker's yeast Saccharomyces cerevisiae. (wikipedia.org)
Herein, we used the amenable yeast Saccharomyces cerevisiae to study whether ubiquitin facilitates the expression of the fused eL40 (Ubi1 and Ubi2 precursors) and eS31 (Ubi3 precursor) ribosomal proteins. (123dok.net)
In the model yeast Saccharomyces cerevisiae, Trk1 is the main K+ importer. (bvsalud.org)
Throughout history, the yeast Saccharomyces cerevisiae has played a central role in human society due to its use in food production and more recently as a major industrial and model microorganism, because of the many genetic and genomic tools available to probe its biology. (asm.org)
However, major genetic engineering limitations have kept this yeast from replacing the commonly used yeast Saccharomyces cerevisiae in industrial applications. (asm.org)
The yeast Saccharomyces cerevisiae has served as the major eukaryotic organism for synthetic biology, but lacks the metabolic potential that could be exploited in many of the more than one thousand yeast species that have been identified to date. (asm.org)

Mitochondrial distribution and morphology protein 12 OS=Schizosaccharomyces pombe (strain 972 / ATCC. (yeastrc.org)

Yeasts differ in productivity and with respect to their capabilities to secrete, process and modify proteins. (wikipedia.org)
Yeasts are common hosts for the production of proteins from recombinant DNA. (wikipedia.org)
Using these data, it was discovered that gene expression by non-Saccharomyces yeasts and initial nitrogen content in grape musts are correlated with differences in gene expression among fermentations. (bvsalud.org)
Yeasts like Saccharomyces cerevisiae metabolize carbohydrates into carbon dioxide and alcohols via fermentation, a process integral to baking and alcoholic beverage production. (microbiologynote.com)

S. cerevisiae is also a critical model organism in cell biology research, aiding in the understanding of eukaryotic cell and human biology. (microbiologynote.com)

Saccharomyces cerevisiae is the traditional baker's yeast used widely in brewing and baking. (wikipedia.org)

It is generated by proteolytic cleavage of precursor proteins in which it is fused either to itself, constituting a polyubiquitin precursor of head-to-tail monomers, or as a single N-terminal moiety to ribosomal proteins. (123dok.net)
Understanding the role of the ubiquitin fused to ribosomal proteins becomes relevant, as these proteins are practically invariably eS31 and eL40 in the different eukaryotes. (123dok.net)
We conclude that ubiquitin might serve as a cis-acting molecular chaperone that assists in the folding and synthesis of the fused eL40 and eS31 ribosomal proteins. (123dok.net)
In most eukaryotes studied, ubiquitin is encoded by two classes of genes: (i) The first comprises genes coding for a single copy of ubiquitin fused to ribosomal proteins (r-proteins), most commonly eL40 and eS31. (123dok.net)
Ubiquitin fusion to other r-proteins, such as P1 or P2, and even to non-ribosomal proteins such as actin, has been reported in diverse genera of rare single-celled algae as, for example, Bigelowiella [6]. (123dok.net)

Induction of the reporter gene and protein-binding was dependent on the presence of a functional KlCAT8 gene, suggesting that, in K. lactis, K1Cat8p acts in the transcriptional regulation of respiratory function. (ox.ac.uk)

In accordance with the high homology level of the secretory pathway proteins in different yeast species, the polyclonal antibodies raised against S. cerevisiae Seb1p, Sso2p and Sec4p can detect homologous proteins in cell lysates of K. lactis and Pichia pastoris, the latter also in Candida utilis. (vtt.fi)
Like other methylotrophic species such as Hansenula polymorpha and Pichia pastoris, it is used as a platform for the production of foreign proteins. (wikipedia.org)

A Candida albicans gene (CPH1) was cloned that encodes a protein homologous to Saccharomyces cerevisiae Ste12p, a transcription factor that is the target of the pheromone response mitogen-activated protein kinase cascade. (embl-heidelberg.de)
Peptide determinants of RF1 and RF2 involved in the specificity of stop codon recognition have been identified: in both cases a tripeptide (PAT for RF1 and SPF for RF2) was located in the homologous region of both proteins [ 14 ]. (biomedcentral.com)

As eukaryotes, they are able to perform protein modifications like glycosylation which are common in eukaryotic cells, but relatively rare in bacteria. (wikipedia.org)
Ubiquitin is a highly conserved small eukaryotic protein. (123dok.net)
Ubiquitin is a small eukaryotic protein of 76 amino acids whose name (i.e., it occurs ubiquitously) results from its remarkable evolutionary conservation [1]. (123dok.net)
The smaller proteins are generally of prokaryotic origin while the larger ones are of eukaryotic origin. (tcdb.org)
The larger eukaryotic and archaeal proteins possess N- and C-terminal hydrophilic extensions. (tcdb.org)

This work aimed to investigate the effect of dietary Lactococcus lactis and Bacillus subtilis on the immune responses, intestinal microbiota, and resistance to pathogens of Oscar, Astronotus ocellatus . (ij-aquaticbiology.com)

Nonconventional yeast species are attracting attention due to their unique properties and as a potential source of genes that encode proteins with unusual characteristics. (bvsalud.org)
Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for MDM12 . (yeastrc.org)
Surprisingly, some of the evolutionarily youngest regions in two of the eleven species ( S. cerevisiae and K. waltii ) are shorter than the lower limit observed in older regions. (biomedcentral.com)
Genes encoding proteins from the anthocyanin synthesis pathway derived from the species Lupinus angustifolius (narrow-leaved blue lupine) with a spectrum of blue anthocyanins will be explored. (lu.lv)
The term "yeast" is frequently synonymous with S. cerevisiae, though this does not reflect the full phylogenetic diversity of yeast species. (microbiologynote.com)
Yeast, notably Saccharomyces cerevisiae, is often used synonymously with the term 'yeast', but it is just one of many species within the diverse Ascomycota and Basidiomycota phyla. (microbiologynote.com)

A once-daily servingin one small, easy-to-swallow capsule Has 34 RAW probiotic strains promoting flora diversity as found in nature Features the clinically studied Replenish Blend to restore the good bacteria lost* Provides protein-digesting enzymes for added digestive support* Arrive Alive! (wellbeinggps.com)
Examination of the potential of Trks to support the stress resistance of S. cerevisiae wild-type strains showed that Y. lipolytica Trk1 is a promising tool for improving cell tolerance to both low K+ and high salt and that the overproduction of S. cerevisiae's own Trk1 was the most efficient at improving the growth of cells in the presence of highly toxic Li+ ions. (bvsalud.org)
Commercial exploitation of yeast began in the late 18th century, with strains like S. cerevisiae and S. pastorianus being identified for brewing. (microbiologynote.com)

2007). Quercetin increases oxidative stress resistance and longevity in Saccharomyces cerevisiae . (up.pt)
2012). Quercetin Protects Saccharomyces cerevisiae against Oxidative Stress by Inducing Trehalose Biosynthesis and the Cell Wall Integrity Pathway . (up.pt)

A yeast expression platform is a strain of yeast used to produce large amounts of proteins, sugars or other compounds for research or industrial uses. (wikipedia.org)
During fermentation, Saccharomyces cerevisiae metabolizes sugars and other nutrients to obtain energy for growth and survival, while also modulating these activities in response to cell-environment interactions. (bvsalud.org)

The secretion of recombinant proteins into the extracellular space by Escherichia coli presents advantages like easier purification and protection from proteolytic degradation. (uni-bielefeld.de)
R.S.V. Selvamani, K. Friehs, and E. Flaschel, "Extracellular recombinant protein production under continuous culture conditions with Escherichia coli using an alternative plasmid selection mechanism", Bioprocess and Biosystems Engineering , vol. 37, 2014, pp. 401-413. (uni-bielefeld.de)

Overexpression of these genes can rescue temperature‐sensitive (ts) growth defect of many sec mutants impaired in protein secretion. (vtt.fi)
The production of recombinant mature hormone in suitable hosts and secretion of this therapeutic protein into the extracellular space can be considered as one of the best cost-effective approaches not only to obtain the active form of the protein but also endotoxin-free preparation. (biomedcentral.com)
The plasmid stability in a continuously cultivated E. coli fermentation, in the absence of antibiotics, with extracellular secretion of recombinant protein provides an interesting model for further improvements. (uni-bielefeld.de)

Enzymatic activity of the recombinant proteins will be tested with individual substrates and their natural mixtures (deproteinated plant extracts) used in industry (for the list of suppliers, refer to). (lu.lv)

In the form of a once-daily, easy-to-swallow capsule, RAW Probiotics Ultimate Care also contains protein-digesting enzymes for added digestive support along with help relieving symptoms such as gas, constipation and diarrhea. (vitaminlife.com)
Industrially, yeast plays a pivotal role in baking and brewing, and also serves as a source for protein, biofuel production, and the commercial synthesis of various enzymes and organic compounds. (microbiologynote.com)

Thus, we demonstrate a coupling in alpha cells between a protein functioning in cell identity, alpha 1, with a protein responsive to the pheromone-induced signal STE12. (embl-heidelberg.de)
Specific and differential protein-binding to the activating sequence was observed with extracts from glucose- and ethanol/glycerol-grown cells. (ox.ac.uk)
2016). Ceramide signaling targets the PP2A-like protein phosphatase Sit4p to impair vacuolar function, vesicular trafficking and autophagy in Isc1p deficient cells . (up.pt)
The recombinant yeast cells or purified proteins will be used for biosynthesis of anthocyanins. (lu.lv)
Saccharomyces cerevisiae cells in DIC microscopy. (microbiologynote.com)

The ScTrk1 protein can be of great interest to industry, as it was shown that changes in its activity influence ethanol production and tolerance in S. cerevisiae and also cell performance in the presence of organic acids or high ammonium under low K+ conditions. (bvsalud.org)

Several of them are also applied to genetic engineering and to the production of foreign proteins. (wikipedia.org)
Recent advances in genetic engineering have made it possible for microorganisms to be used for expression of heterologous proteins. (biomedcentral.com)

As CXXC1 is orthologous to Saccharomyces cerevisiae Spp1 that links DSB sites to the DSB machinery on the chromosome axis, we propose that these molecular interactions involved in the regulation of meiotic DSB formation are conserved in mouse meiosis. (springer.com)
These glycoproteins include the CD98 heavy chain protein of Mus musculus (gbU25708) and the orthologous 4F2 cell surface antigen heavy chain of Homo sapiens (spP08195). (tcdb.org)

Here, differences in S. cerevisiae gene expression were explored over a time course of fermentation and used to differentiate fermentations, using Pinot noir grapes from 15 unique sites. (bvsalud.org)
Using this method, site-specific deviations in gene expression were identified, including changes in gene expression correlated with the non-Saccharomyces yeast Hanseniaspora uvarum, as well as initial nitrogen concentrations in grape musts. (bvsalud.org)
These results highlight novel relationships between site-specific variables and Saccharomyces cerevisiae gene expression that are linked to repeated fermentation outcomes. (bvsalud.org)
It was also demonstrated that DMap-DE can extract biologically relevant gene expression patterns from other contexts (e.g., hypoxic response of Saccharomyces cerevisiae) and offers advantages over other data dimensionality reduction approaches, indicating that DMap-DE offers a robust method for investigating asynchronous time series gene expression data. (bvsalud.org)
IMPORTANCE In this work, Saccharomyces cerevisiae gene expression was used as a biosensor to capture differences across and between fermentations of Pinot noir grapes from 15 unique sites representing eight American Viticultural Areas. (bvsalud.org)

Furthermore, their overexpression in wild‐type yeast enhances production of secreted proteins in S. cerevisiae, which suggests that they may be rate‐limiting factors in this process. (vtt.fi)
2006). The Pep4p vacuolar proteinase contributes to the turnover of oxidized proteins but PEP4 overexpression is not sufficient to increase chronological lifespan in Saccharomyces cerevisiae . (up.pt)
In this respect, although obviously lacking a brain with a multicellular neuronal network, simple unicellular yeast are of great value to investigate the effects of overexpression and single point-mutations in genes encoding key proteins on cell physiology and on the function of conserved signaling cascades. (microbialcell.com)

Ubiquitin functions as a reversible post-translational modifier of proteins to regulate many different cellular processes such as DNA repair, chromatin dynamics, cell cycle regulation, membrane and protein trafficking, endocytosis, autophagy, but most notably proteasome-dependent protein degradation [2-4]. (123dok.net)
The controlled co-expression of a bacteriocin release protein aids in moving periplasmic proteins through the outer membrane. (uni-bielefeld.de)
15. KulkarniRD, KelkarHS, DeanRA (2003) An eight-cysteine-containing CFEM domain unique to a group of fungal membrane proteins. (prelekara.sk)
Furthermore, 'cell membrane' display of target proteins (Hara et al. (springeropen.com)
2012 ) has been achieved by fusing the target proteins to plasma membrane proteins such as Yps1p (Gagnon-Arsenault et al. (springeropen.com)

For example, it can have inhibitory effects on glycolysis followed by a direct effect on protein synthesis [ 2 ], as well as increase the absorption and retention of calcium, magnesium and phosphate ions in the body. (biomedcentral.com)
The work continued with sequencing the fragments encoding the proteins of the anthocyanin synthesis pathway. (lu.lv)
Termination of protein synthesis in eukaryotes involves at least two polypeptide release factors (eRFs) - eRF1 and eRF3. (biomedcentral.com)
Termination of protein synthesis occurs when the ribosome elongation machinery encounters an in-frame termination (stop) codon, either UAG, UGA or UAA, in the mRNA. (biomedcentral.com)

The yeast cell wall is a complex structure comprising proteins, lipids, and at least two types of polysaccharides: mannan and glucan. (microbiologynote.com)

These sequences represent the protein coding region of the COX15 cDNA ORF which is encoded by the open reading frame (ORF) sequence. (genscript.com)
Specific primers were designed and used for obtaining 3' sequences of six proteins from four plants having blue flowers or berries (Centaurea cyanus, Cichorium intybus, Vaccinium uliginosum and Vaccinium myrtillus). (lu.lv)

Since the native signal protein peptide of S. aureus protein A was not able to deliver hGH to the extracellular space, it was modified using bioinformatics tools and fused to the n-terminal region of hGh to show that the redesigned signal peptide was functional. (biomedcentral.com)

In the past decade, yeast have been frequently employed to study the molecular mechanisms of human neurodegenerative diseases, generally by means of heterologous expression of genes encoding the relevant hallmark proteins. (microbialcell.com)

Title: Regulation of the heme A biosynthetic pathway: differential regulation of heme A synthase and heme O synthase in Saccharomyces cerevisiae. (genscript.com)
Here we show that efficient heme uptake relies on a family of extracellularly-anchored proteins that serve as heme receptors, two of which, at least, are required for efficient heme utilization. (prelekara.sk)
Our data suggest the existence of a relay system that transfers heme from one protein to the next across the cell envelope, explaining the requirement for multiple heme receptors for efficient heme-iron utilization. (prelekara.sk)
A Relay Network of Extracellular Heme-Binding Proteins Drives Iron Acquisition from Hemoglobin. (prelekara.sk)

The highly conserved translation termination factor eRF1 in Saccharomyces cerevisiae is encoded by the essential gene SUP45 . (biomedcentral.com)

Mitochondrial distribution and morphology protein 12 OS=Candida glabrata (strain ATCC 2001 / CBS 138. (yeastrc.org)
Mitochondrial distribution and morphology protein 12 OS=Candida albicans (strain SC5314 / ATCC MYA-2. (yeastrc.org)
14. WeissmanZ, KornitzerD (2004) A family of Candida cell surface haem-binding proteins involved in haemin and haemoglobin-iron utilization. (prelekara.sk)
In addition, S. fibuligera was cultivated alone on starchy waste in Czechoslovakia or in mixed culture with Candida utilis on potato-processing wastes in Sweden to produce single-cell protein, which was used for protein supplementation in animal feeds [ 10 ]. (biomedcentral.com)

They are members of the rBAT family of mammalian proteins (TC #8.A.9). (tcdb.org)

The results showed that adding B. subtilis and L. lactis , particularly in MIX 300 , reduced the anaerobic heterotrophic bacterial microbiota and increased lactic acid bacteria (LAB) in fish. (ij-aquaticbiology.com)

The SEB1/SBH1 and the SSO genes encode components of the protein secretory machinery functioning at the opposite ends, ER translocation and exocytosis, respectively, of the secretory pathway in Saccharomyces cerevisiae. (vtt.fi)

6.Saccharomyces cerevisiae is used in -- fermentation. (mcques.in)

Protein-Digesting Enzyme Blend Protease, Acid Protease and Protease S. (vitaminlife.com)

The QCR8 gene of the yeast K1uyveromyces lactis is transcriptionally regulated by the carbon source in the growth medium. (ox.ac.uk)
The activating element contains no other known regulatory sites but two elements required for RNA holoenzyme functioning, raising the intriguing possibility of carbon source-dependent regulation by a subunit of the RNA polymerase holoenzyme in K. lactis. (ox.ac.uk)
However, S. cerevisiae has proven difficult to engineer to expand the carbon sources it can utilize, the products it can make, and the harsh conditions it can tolerate in industrial applications. (asm.org)

Instead, the MAT alpha-encoded protein alpha 1 and the MCM1 product bind to the DNA element that is responsible for alpha-specific and a-factor-inducible expression. (embl-heidelberg.de)
A time lapse experiment of Saccharomyces cerevisiae expressing GFP-tagged MCM1. (ucsd.edu)

Mitochondrial distribution and morphology protein 12 OS=Ashbya gossypii (strain ATCC 10895 / CBS 109. (yeastrc.org)
Mitochondrial distribution and morphology protein 12 OS=Yarrowia lipolytica (strain CLIB 122 / E 150. (yeastrc.org)

Normally, the conjugation of ubiquitin to other proteins involves the formation of an isopeptide bond between the α-carboxyl group of the C-terminal glycine of a ubiquitin molecule with an ε-amino group of a specific lysine residue within the target protein [2,3]. (123dok.net)

Anatomy20

Organisms15

Diseases1

Chemicals and Drugs116

Analytical, Diagnostic and Therapeutic Techniques and Equipment23

Phenomena and Processes89

Disciplines and Occupations1

Technology, Industry, Agriculture3

Information Science4

Health Care3

Kluyveromyces13

S288c1

Yeast Saccharomyces cerevisiae6

Schizosaccharomyces1

Yeasts4

Organism1

Baker's yeast1

Ribosomal proteins5

Transcriptional1

Pichia2

Homologous2

Eukaryotic5

Lactococcus1

Species6

Strains3

Oxidative Stress2

Sugars2

Escherichia2

Secretion3

Recombinant proteins1

Enzymes2

Cells5

Ethanol1

Genetic2

Orthologous2

Gene expression5

Overexpression3

Membrane5

Synthesis4

Lipids1

Sequences2

Extracellular space1

Mechanisms1

Heme4

Highly conserved1

Candida4

Mammalian1

Bacteria1

Encode1

Fermentation1

Protease1

Carbon3

MCM12

Mitochondrial2

Involves the formation1