• DVTP nick-end labeling (TUNEL) assay with the In Situ Cell determined according to the manufacturer's protocol. (cdc.gov)
  • The nucleotide also serves as a substrate for terminal transferase in 3′-end labeling and TUNEL. (sigmaaldrich.com)
  • The cell viability and apoptosis were determined by using adenosine 5′-triphosphate (ATP) assay and terminal deoxynucleotidyl transferase (TdT)-mediated deoxyuridine triphosphate (dUTP) nick end labeling (TUNEL), respectively. (molvis.org)
  • FD NeuroApop™ Kit is specifically designed for the detection of neuronal apoptosis in tissue sections from the central nervous system based on the principle of in situ DNA nick-end labeling (TUNEL) technique. (thomassci.com)
  • Terminal deoxynucleotidyl-mediated dUTP nick end labeling (TUNEL) was used to study cellular apoptosis. (atsjournals.org)
  • Fluorescein-12-dUTP has been used to label nuclear extracts during PCR. (sigmaaldrich.com)
  • Repeated fluorescence labeling using Tetramethyl-Rhodamine-6-dUTP (red) and AMCA-6-dUTP (bright blue) is possible. (sigmaaldrich.com)
  • Cells undergoing DNA fragmentation were quantified by performing in situ 3′ end labeling of DNA with digoxygenin-dUTP on ovarian sections. (uba.ar)
  • En face co-immunostaining of the mouse aortic arch revealed a low level of PDCD4 in endothelial cells undergoing pulsatile shear stress. (plos.org)
  • Interestingly splenocytes were labelled positive for apoptosis more often than hepatocytes in both MPXV groups. (hindawi.com)
  • An in situ method for detecting areas of DNA which are nicked during APOPTOSIS . (nih.gov)
  • The terminal deoxynucleotidyl transferase nick end labeling, or TUNEL , assay labels apoptosis on a single-cell level, making it more sensitive than agarose gel electrophoresis for analysis of DNA FRAGMENTATION . (nih.gov)
  • Apoptosis was quantified by DNA-fragment terminal labeling. (highwire.org)
  • TUNEL reaction preferentially labels DNA strands that generated during apoptosis using the ability of TdT to label blunt ends of double-stranded DNA independent of a template. (bioacts.com)
  • This sive labeling allows discrimination of apoptosis from necrosis and from primary DNA strand breaks induced by cytostatic drugs or irradiation. (bioacts.com)
  • Terminal deoxynucleotidyl transferase is used to add labeled dUTP, in a template-independent manner, to the 3 prime OH ends of either single- or double-stranded DNA . (nih.gov)
  • Whichever the choice of labeling strategy, the double-stranded DNA probes need to be denatured into single-stranded DNA so that they can bind the target DNA. (jove.com)
  • DNA probes can be used to achieve this - these are single-stranded DNA molecules that are complementary to the target nucleic acid sequence and labeled with a detection molecule. (jove.com)
  • The most commonly used labels for DNA probes include radioisotopes like Phosphorus 32, fluorophores like fluorescein, or small molecule binding partners such as biotin and digoxigenin. (jove.com)
  • These labels can be incorporated chemically after the synthesis of DNA probes or during their synthesis via one of the two methods. (jove.com)
  • For internal labeling, the nick translation method uses the DNase enzyme to make a small nick in one of the DNA strands followed by DNA polymerase I - which adds labeled and unlabeled nucleotides directly into the DNA strand. (jove.com)
  • For example, radioisotopes are highly sensitive labels, and they can be directly detected through autoradiography. (jove.com)
  • Normal live cell is not labeled by ether Annexin V or PI, early stage apoptotic cell is stained with Annexin V and natural death or cell subject to necrosis is stained with PI. (bioacts.com)
  • In the end-labeling method, the hydroxyl or phosphate of the nucleotide present at the 5' or 3' termini of the probe DNA sequence is covalently bound to the desired chemical group or label. (jove.com)
  • The selection of the specific probe label is based on a variety of factors, including sensitivity, safety, probe stability, and method of detection. (jove.com)
  • This chemo-immunotherapy that concentrates DCs to present endogenous tumor antigens generated in situ may broadly serve as a facile platform to modulate the suppressive TME, and enable in situ personalized cancer vaccination. (nature.com)