• Studies of lymphocyte mitogenic function in 45 exposed Michigan dairy farm ers showed normal responses to phytohemagglutinin (PHA) and pokeweed mitogen (PWM) and a reduced reactivity in mixed lymphocyte cultures in 27 subjects. (cdc.gov)
  • After several days, lymphocytes underwent blast transformation, DNA synthesis and cellular proliferation in response to the major histocompatibility antigen (MHC Class I and II) differences between the two cell populations designated as Responder and Stimulator cells. (wikipedia.org)
  • The purified BDC elicited strong allogeneic mixed leukocyte reactions and HLA classes II- and I-restricted antigen-specific primary immune responses. (nih.gov)
  • It may be nonspecific as in the administration of immunosuppressive agents (drugs or radiation) or by lymphocyte depletion or may be specific as in desensitization or the simultaneous administration of antigen and immunosuppressive drugs. (lookformedical.com)
  • Human alveolar macrophages from bronchoalveolar lavage are usually poor accessory cells for antigen-induced T-lymphocyte proliferation and poor stimulators of allogeneic mixed leukocyte reactions (MLR) when compared to peripheral blood monocytes. (elsevierpure.com)
  • Quality Assurance also includes testing for sterility, pH, osmolority, presence, or absence of Mycoplasma, endotoxin and presence of Human Leukocyte Antigen (HLA Ag) by Complement Dependent Cellular Cytotoxicity (CDCC) and flow cytometry. (labquip.net)
  • This material was tested and found to be non-reactive for hepatitis B Surface Antigen (HBsAg), Anti-HIV and Hepatitis C. (labquip.net)
  • In children who have completed immunizations with diphtheria, pertussis, and tetanus (DPT) or Hib-conjugated vaccines, the antibody response to protein antigens can be tested in adults and older children by measuring IgG antibodies to tetanus or diphtheria toxoid and H influenzae type b (Hib) polysaccharide antigen. (medscape.com)
  • Hepatitis B is not a reliable antigen for testing immune competence because of the high frequency of nonresponders in the population, particularly in persons older than 40 years. (medscape.com)
  • The patient was subjected to immunological tests (i.e., purified protein derivative, venereal disease research laboratory, anti-human immunodeficiency virus 1/2, anti-hepatitis B surface antigen, anti-hepatitis B core, anti-Epstein-Barr virus, Chagas [ELISA]) and was diagnosed nonreactive. (jcadonline.com)
  • Borrelia burgdorferi LFA-1 (Lymphocyte Function Antigen 1): Own body protein + Borrelia burgdorferi sensu stricto (shared epitope). (arminlabs.com)
  • The in vitro effects of MSC-Exo on immune cell migration and responder T cell proliferation were examined by chemotactic assays and lymphocyte proliferation assays, respectively. (nature.com)
  • Using a large screening of chemicals and natural compounds already approved by the U.S. Food and Drug Administration or tested in previous studies we identified one chemical substance called CGS- 15943 which has an effect in improving the proliferation of normal cells by decreasing the number of PNH cells. (aamds.org)
  • PTX and several cogeners were found to directly suppress T cell proliferation in response to phytohemagglutinin, to allogeneic cells in a mixed leukocyte reaction, and to cross-linking the CD3 complex. (elsevierpure.com)
  • The bone marrow- and adipose tissue-derived MSCs were cultured under human platelet lysate conditions and their biological characteristics evaluated for cell therapy (morphology, immunophenotype, colony-forming unit-fibroblast efficiency, proliferation capacity, potential for mesodermal differentiation, secreted proteins, and immunomodulatory effects). (biomedcentral.com)
  • Technically, it is an ex-vivo cellular immune assay that occurs between two allogeneic lymphocyte populations (same species but genetically distinct). (wikipedia.org)
  • Further characterization of the lymphocytes, accessory cells (dendritic cells, macrophage) and cytokines that participate in the MLR have been done as this assay continues to be used to define mechanisms for understanding cellular immune function in vitro. (wikipedia.org)
  • The frequencies of chromosome aberrations in both vehicle and positive controls were within the expected range and verified the sensitivity of the assay and the efficacy of the S9-mix. (europa.eu)
  • Based on the results from the preliminary toxicity test, the doses selected for treatment of the initial mutagenesis assay ranged from 2.5 to 120 µg/mL and 20 to 160 µg/mL for the S9 non-activated and activated cultures, respectively. (europa.eu)
  • Based on the results of the preliminary toxicity test, the doses chosen for treatment of the extended treatment assay ranged from 0.31 to 15 µg/mL for non-activated cultures with a 24-hour exposure. (europa.eu)
  • Additionally, MarinBio specializes in in vitro cell-based assay testing services for clinical and pre-clinical studies, including method development, qualification, validation, transfer, lot-release testing, and potency testing according to guidelines from both FDA and International Council for Harmonization (ICH). (marinbio.com)
  • Methods We used several in vitro methods including Caco-2/THP-1 leaky gut model, AhR activation, PBMC assay and mixed lymphocyte reaction (MLR) to assess the impact of a MET-C candidate (MaaT03X) on gut homeostasis and immune activation. (bmj.com)
  • Under human platelet lysate-supplemented culture conditions, bone marrow- and adipose tissue-derived MSCs exhibited similar fibroblast-like morphology and expression patterns of surface markers. (biomedcentral.com)
  • There is, at most, mixed evidence that THC and other cannabinoids are mutagenic in standard microbial assays, such as the Ames test, and there is contradictory evidence on whether the cannabinoids are clastogenic, i.e. produce breaks in chromosomes. (druglibrary.org)
  • Lymphocytes (0.4 mL blood of a healthy male donor was added to 5 mL or 4.8 mL culture medium, without and with metabolic activation respectively and 0.1 mL (9 mg/mL) Phytohaemagglutinin) were cultured for 48 h and thereafter exposed to selected doses of test substance for 3 h, 24 h and 48 h in the absence of S9-mix or for 3 h in the presence of S9-mix. (europa.eu)
  • Under the study conditions, the test substance is not considered to be mutagenic in the presence and absence of exogenous metabolic activation. (europa.eu)
  • During the study test strains of Salmonella typhimurium (TA98, TA100, TA1535 and TA1537) and a tester strain of Escherichia coli (WP2uvrA) were exposed to the test substance both in the presence and the absence of metabolic activation. (europa.eu)
  • No toxicologically significant increases in the frequency of revertant colonies were recorded for and of the bacterial strains, with any dose of the test substance, with or without metabolic activation. (europa.eu)
  • Three treatment conditions were used for the study, as follows: firstly, cultures were exposed for 4 hours with a 20 hour expression time, both in the presence and absence of metabolic activation (S9 mix), secondly cultures were continuously exposed for 24 hours in the absence of metabolic activation. (europa.eu)
  • Precipitate of the test substance was observed at and above 10 µg/mL in the 4-hour exposure groups in the absence of metabolic activation and at and above 20 µg/mL in the 4-hour exposure group in the presence of metabolic activation. (europa.eu)
  • Toxicity in the cloned cultures was observed at doses at 120 and 160 µg/mL without and with S9 activation, respectively. (europa.eu)
  • Put simply, it is mixing populations of T-lymphocytes (category of white blood cells) together, and measuring the reaction that occurs. (wikipedia.org)
  • No MLR occurred when the Responder cells came from thymectomized animals, whereas bursectomized chicken leukocytes reacted in culture demonstrating that T-cells were the major cell type in Responder cell populations. (wikipedia.org)
  • Stimulator cell populations that also contain T-cells (Two way mixed lymphocyte reaction) will replicate in the presence of the Responder cells, therefore for a One way mixed lymphocyte reaction, stimulator cells are prevented from replicating by irradiation or treatment with mitomycin C, a DNA crosslinker to prevent cell replication. (wikipedia.org)
  • After a second centrifugation step, HBSS was removed and cells were resuspended in 5 mL culture medium and incubated for another 20 - 22 h (24 h fixation time). (europa.eu)
  • Periodically 'cleansed' of spontaneous mutants: Prior to dose-range finding and mutagenicity testing, the mouse lymphoma cells were grown for 1 day in R10-medium containing hypoxanthine, aminopterine and thymidine to reduce the amount of spontaneous mutants, followed by a recovery period of 2 days on R10-medium containing hypoxanthine and thymidine only. (europa.eu)
  • Cells were exposed to the test item in basic medium supplemented with 5%/10% (v/v) heat-inactivated horse serum (R5-medium) for 3/24 hour exposure. (europa.eu)
  • The potential of the test substance, manganese dichloride, to induce structural chromosomal aberrations in human lymphocyte cells in vitro was investigated in a study which was conducted under GLP conditions and in accordance with the standardised guideline OECD 473. (europa.eu)
  • During the study duplicate cultures of human lymphocyte cells, treated with test substance, were evaluated for chromosome aberrations over at least three dose levels. (europa.eu)
  • The test substance did not induce any toxicologically significant increases in the frequency of cells with aberrations in either of the 4(20)-hour exposure groups, in the absence or presence of a liver enzyme metabolising system, or following 24 hours continuous exposure. (europa.eu)
  • DC were found to be potent stimulators of T cells in allogeneic and, to a lesser extent, in autologous mixed leukocyte reaction (MLR). (amsterdamumc.org)
  • In six patients and six controls allogenic mixed leucocyte reaction (AMLR) was performed using mDC and cord blood-derived naive T cells at a DC/T naive ratio of 1 : 200. (torvergata.it)
  • Moreover, proliferative response of allogenic naive T cells cultured with mDC was impaired in the patients (13471 +/- 9917.2 versus 40976 +/- 24527.2 cpm, P = 0.04). (torvergata.it)
  • CTLL-2 cells were cultured with increasing concentrations of client drug for four days. (marinbio.com)
  • MarinBio's contract research and development service professionals are expert in designing customized or standardized bioassays and in the measurements of interactions of drugs (including small molecules, biologics, or device interactions) with cultured cells and primary cells. (marinbio.com)
  • Our proposal focuses on the possibility to test agents selectively reducing the fraction of GPI-AP deficient cells and consequently improving the growth of GPI-AP normal cells. (aamds.org)
  • Our proposal is based on the possibility to pre-clinically test drugs improving the function of normal cells and decreasing the fractions of cells with PIGA mutations. (aamds.org)
  • Loosely adherent cells (LAM) were obtained after an overnight incubation on plastic culture dishes of initially adherent mononuclear cells. (elsevierpure.com)
  • Synergy among thymocytes and peripheral lymph node cells in the in vitro generation of lymphocytes cytotoxic to alloantigens. (ox.ac.uk)
  • In the course of the acute immune response, the T lymphocytes first differentiate into effector T cells, which are actively involved in the elimination of virus-infected cells (CD8 killer T cells) and in controlling the function of other immune cells (CD4 T helper cells). (drroberthess.com)
  • The ELISPOT method, on which our SARS-CoV-2 T-Cell immunity test is based, provides a sensitive and specific analysis of T cells in the blood samples submitted by our clients. (drroberthess.com)
  • This cell culture test is based on the release of messenger substances from the immune system (cytokines) by single cells after stimulation of lymphocytes with virus fragments (peptides). (drroberthess.com)
  • Besides CD19 + CD138 − B cells, plasmablasts, and plasma cells were enriched in the tongue of mice colonized with C. albicans suggesting a potential role of B lymphocytes during oral fungal colonization. (frontiersin.org)
  • Chromosomal aberrations (hypo- and hyperdiploidy, deletions, breaks, and gaps) in peripheral lymphocytes and bone marrow cells are the predominant effects seen in humans. (cdc.gov)
  • A novel retrovirus, human T -celllymphomaleukemia virus (HTLV), has been isolated from the fresh and cultured cells of two of these patients. (science-connections.com)
  • Subsequent characterization of this virus has shown that it is not significantly related to any known animal retrovirus, is not an endogenous (genetically transmitted) virus of man, and so far has been associated only with fresh or cultured T -cells from patients with T -cell neoplasia. (science-connections.com)
  • 1975) have required the establishment of continuously growing cell lines from the disease of origin or cocultivation of these cells with previously established cell lines, the ability to grow malignant T -cells in long-term culture could facilitate the isolation of retroviruses from these diseased states. (science-connections.com)
  • The basic and common immunologic defect in common variable immunodeficiency is a failure of B-lymphocyte differentiation into plasma cells that produce the various immunoglobulin (Ig) isotypes. (medscape.com)
  • There is experimental evidence from in vitro studies of animal cells that some cannabinoids, including THC, can produce a variety of changes in cellular processes in vitro (i.e. in the test tube). (druglibrary.org)
  • The protocol of Dr. Collins on the web site also discusses the use of lymphocytes for cells and/or UV treatment of these. (neb.com)
  • Treat cultured mouse fibroblast (NIH 3T3) or osteocyte cells with trypsin to detach the cells from the dish. (neb.com)
  • Results from this experiment were confirmed by those of a second test with groups of female mice of the same strain fed dietary levels of captan ranging from 500 to 16 000 ppm for up to 213 days. (inchem.org)
  • Alongside the production by B lymphocytes of virus-specific antibodies, the stimulation and multiplication (clonal expansion) of virus-specific T lymphocytes is a central feature of this immune response. (drroberthess.com)
  • The confirmation by this cell function test of prior Covid-19 immunization succeeds significantly more often than with the serological determination of antibodies, as SARS-CoV-2-specific antibodies are not always detectable even with a PCR test following an infection. (drroberthess.com)
  • Borrelia infections do not only show humoral immune responses by antibodies, but can activate T-lymphocytes at the same time. (arminlabs.com)
  • Laboratory tests showed leukocytosis (20.79 × 10 9 /L), moderate anemia (69 g/L), increased percent of neutrophils (91.6%), increased C-reactive protein (CRP) (80.90 mg/L), procalcitonin (PCT) (5.87 ng/ml) and erythrocyte sedimentation rate (ESR) (106.0 mm/h) level, and mild hypoalbuminemia (31.0 g/L). Stool routine revealed Leukocyte ++++/HP, pus cell ++++/HP, stool blood was positive. (frontiersin.org)
  • In the other 18 subjects, the responses to PHA and PWM were significantly reduced along with that of mixed leukocyte cultures. (cdc.gov)
  • 1963. A reaction between leukocytes in mixed peripheral blood cultures. (wikipedia.org)
  • This study was taken up to evaluate the antigenotoxic potential of ginger in human peripheral blood lymphocytes. (scialert.net)
  • In vitro treatment of peripheral blood lymphocytes with TSO induced significant number of micronuclei in all samples. (scialert.net)
  • mDC, derived from peripheral blood monocytes cultured for 6 days in presence of interleukin (IL)-4 and granulocyte-macrophage colony stimulating factor (GM-CSF) and stimulated with lipopolysaccharide (LPS) for the last 24 h, were phenotyped for the expression of the co-stimulatory molecules B7.1 and B7.2. (torvergata.it)
  • Translocations in peripheral blood lymphocytes were scored by using fluorescence in situ hybridisation whole-chromosome painting. (cambridge.org)
  • Similar results were obtained using biotinylated CMRF-56 mAb, an antibody identifying a comparable population in cultured PBMC. (nih.gov)
  • So far, we have been testing only one of the two immunity pillars, namely antibody production. (drroberthess.com)
  • The effect of antilymphocyte antibody on lymphocyte transformation. (eurekamag.com)
  • Mixed lymphocyte reaction (MLR) is a test used by pharmaceutical and biotech organizations to show the safety of a drug or implantable material. (wikipedia.org)
  • Aqueous ginger extract was added at concentrations namely 0.1, 0.2 and 0.4 mg to test for antigenotoxicity. (scialert.net)
  • Two separate experiments were conducted, in the first five concentrations of test substance (50, 150, 500, 1500 and 5000 µg/plate) were assayed in triplicate against each tester strain, using the direct plate incorporation method. (europa.eu)
  • Serum PBB concentrations, however, were not correlated with lymphocyte function. (cdc.gov)
  • All of the positive control substances induced marked increases in the frequency of revertant colonies, confirming the activity of the S9-mix and the sensitivity of the bacterial strains. (europa.eu)
  • In the second experiment the test substances and vehicle control were dosed using a pre-incubation method. (europa.eu)
  • Peripheral human lymphocytes are recommended in international guidelines (OECD, EC). (europa.eu)
  • A study was conducted to determine the mutagenic potential of test substance, Coco TMAC (33% active in water), according to OECD 471 and EPA OPPTS 870.5265 Guidelines, in compliance with GLP. (europa.eu)
  • The mutagenic potential of the test substance, manganese dichloride, was investigated in a study which was conducted under GLP conditions and in accordance with the standardised guidelines OECD 471 and EU Method B.13/14. (europa.eu)
  • The mutagenic potential of the test substance, manganese dichloride, was determined in a study which was conducted under GLP conditions and in accordance with the standardised guideline OECD 476. (europa.eu)
  • Different media used in culture processes should maintain the biological characteristics of MSCs during multiple passages. (biomedcentral.com)
  • This study aims to conduct a direct head-to-head comparison of the biological characteristics of the two types of MSCs under human platelet lysate-supplemented culture conditions for their ability to be used in regenerative medicine applications. (biomedcentral.com)
  • MaaT Pharma, a clinical-stage biotech pioneer in the development of Microbiome Ecosystem Therapies TM (MET) in oncology, has developed a unique, ground-breaking, patented pooled co-culture process (MET-C). This technology allows to replicate and leverage, at large industrial scale, the diversity of native-based microbiome ecosystems while tuning the resulting product according to indication-specific compositions. (bmj.com)
  • This OPXV strain (Italy_09/17) was isolated on cell cultures and embryonated eggs. (cdc.gov)
  • Lymphocytes were cultured for 48 ± 2 h and thereafter exposed in duplicate to selected doses of test substance for 3 h in the absence and presence of S9-mix. (europa.eu)
  • Toxicity in the cloned cultures was observed at doses of 10 and 15 µg/mL. (europa.eu)
  • Similar studies conducted in laboratory animals exposed to high and low doses of PBBs (not specified) found a dose related lymphocyte dysfunction in the high dose group. (cdc.gov)
  • Scientists tested their device by infecting rats with lethal doses of either Escherichia coli or Staphylococcus aureus and then cleaning their blood. (discovermagazine.com)
  • Cultured peripheral human lymphocytes were used as test system. (europa.eu)
  • In this study, we tested the effect of local administration of human MSC-Exo on established EAU in the same species. (nature.com)
  • The test substance was therefore considered to be non-clastogenic to human lymphocytes in vitro. (europa.eu)
  • In early tests, the biomechanical treatment removed more than 90 percent of toxins from infected human blood within a few hours. (discovermagazine.com)
  • Researchers then tested their device by cleaning 5 liters of human blood spiked with a variety of pathogens. (discovermagazine.com)
  • The next step for researchers is to test their device in pigs, after which human trials could begin within a couple years, Nature reports. (discovermagazine.com)
  • Bidirectional mixed cultures were performed with lymphocytes from healthy controls and allergic patients with different time periods of IT, and the supernatants obtained from chromatography were tested to determine their effect on IgE synthesis of normal lymphocytes. (cun.es)
  • Suppressor factors derived from allergic patients had no effect on IgE synthesis of normal lymphocytes. (cun.es)
  • It seems that these tests will be used in the future, apart from serological tests. (arminlabs.com)
  • It works by testing specific cellular immunity after a SARS-CoV-2 infection, as well as detecting immune reactivity against endemic coronaviruses, also referred to as background immunity. (drroberthess.com)
  • The MLR was first recognized when researchers mixed leukocytes from two unrelated donors in culture. (wikipedia.org)
  • Therefore, under the conditions of the study the test substance was concluded to be non-mutagenic. (europa.eu)
  • The clinical significance of the changes in lymphocyte function are unknown. (cdc.gov)
  • The test substance was found to cause no visible reduction in growth of the bacterial background lawn at any dose and was therefore tested up to the maximum dose level of 5000 µg/plate A particulate precipitate was at 1500 µg/plate and above. (europa.eu)
  • 0.5 mL of whole blood in a total volume of 5 mL medium was cultured in the presence of 0.16 mM Trans Stilbene Oxide (TSO) for the induction of micronuclei. (scialert.net)
  • This supplementary CE-certified blood test facilitates the cellular immunological detection of a SARS-CoV-2 infection. (drroberthess.com)
  • When those nanobeads are mixed with infected blood they adhere to pathogens, and then, as the blood passes through channels inside the device, magnets pull out the beads with pathogens attached. (discovermagazine.com)
  • The blood culture was also positive for Y. enterocolitica in 1 case. (who.int)
  • The fluorescence-based version of the ELISPOT method used for this test allows for the simultaneous detection of two different cytokines - interferon-y (IFN-y) and interleukin-2 (IL-2). (drroberthess.com)
  • Les numérations plaquettaire et leucocytaire ainsi que la concentration en cytokines (IL-2R, IL-8 et TNF-) ont été me- surées. (who.int)
  • Third, if cannabinoids have immunosuppressive effects, then this would have mixed implications for their therapeutic use. (druglibrary.org)
  • However, the diagnosis can be made upon the onset of ataxia and before the appearance of telangiectasia if confirmed by laboratory tests. (shengsci.com)
  • Based on the results of the three in vitro studies, the test substance is considered to have no genotoxicity potential. (europa.eu)
  • A followup study of 90 Michigan residents with high or low PBB exposures found all parameters of lymphocyte function to be normal. (cdc.gov)
  • The author concludes that PBBs are capable of altering lymphocyte function. (cdc.gov)
  • A: "At the moment, there are cellular based tests on the market. (arminlabs.com)
  • Diagnostic tests generally are required to further support a genetic disorder in a diseased animal. (vin.com)
  • While many mixed breed dogs have a significantly lower degree of inbreeding, many populations such as local stray cat populations may actually have a higher than expected degree of inbreeding. (vin.com)
  • However, dominant disease and polygenetic diseases may be just as likely to be seen in mixed breed populations as they are in more inbred populations depending upon the disease and the population. (vin.com)
  • Earlier studies suggested a primary B-lymphocyte defect as a cause of common variable immunodeficiency in a small group of patients. (medscape.com)
  • Biochemical tests and 16S rDNA analysis of six samples and genomic analysis of one sample confirm the presence of Bisgaard taxon 45. (bvsalud.org)