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  • genetic
  • In principle, if scientists could alter the DNA of a cell that would contribute to a live mouse, they could create an animal with any desired genetic change. (laskerfoundation.org)
  • They contributed to most parts of the mouse, but it proved difficult to produce animals with the EC DNA in egg or sperm, an accomplishment that was essential to breed animals with the genetic change. (laskerfoundation.org)
  • C57BL
  • By capitalizing on efficiencies of scale and a centralized production effort, the project intends to make this catalog of mutants available in mouse strain C57BL/6 for two reasons: it is the most widely used strain, and it is the strain for which complete genome sequence has been made available. (genome.gov)
  • include
  • Examples of research in which knockout mice have been useful include studying and modeling different kinds of cancer , obesity , heart disease , diabetes , arthritis , substance abuse , anxiety , aging and Parkinson's disease . (wikipedia.org)
  • cells
  • For this example, we will take stem cells from a white mouse. (wikipedia.org)
  • Artificial DNA sequences typically are introduced into mouse ES cells using a retrovirus or other viral vector, and the modified ES cells are then grown in cell cultures . (britannica.com)
  • The SAP-deficient mouse model recapitulates several features of XLP: hyperproliferative T cell response following infections, impaired NK and CD8 cell cytotoxicity, defective humoral immune responses, abnormal germinal center formation, reduction in IgG+ memory B cell numbers, and the absence of NKT and other innate T cells. (taconic.com)
  • Scientists could add DNA to mouse cells, but it landed in random places. (laskerfoundation.org)
  • sequence
  • Usually, the new sequence is also given a marker gene , a gene that normal mice don't have and that confers resistance to a certain toxic agent (e.g., neomycin) or that produces an observable change (e.g. colour or fluorescence). (wikipedia.org)
  • research
  • In developing the NIH KOMP plan, this working group considered the current state of the field and recommendations from members of mouse research community made during a second workshop in March 2005. (genome.gov)
  • The University of California, Davis (UC Davis) and Children's Hospital Oakland Research Institute (CHORI) in Oakland, Calif., will collaborate to preserve, protect and make available knockout mice and related products available to the research community. (genome.gov)
  • To collect information generated by the KOMP , track progress of the knockout mutant production pipelines, and make the data readily available to all members of the KOMP research network to support, coordinate, and synergize their individual research programs. (genome.gov)
  • To serve as the central public interface for the KOMP , with links to all groups funded by the KOMP research network, as well as link to other efforts generating knockout mice, such as the European and North American Conditional Mouse Mutagenesis programs (EUCOMM and NORCOMM). (genome.gov)
  • high
  • Support a repository to house the products of this resource as well as an additional 'repatriation' effort to bring into repositories 1000 of the existing high priority mouse knockouts not already stored in a public repository. (genome.gov)
  • study
  • In the present study, we therefore compared blood pressure variability in knockout mice that lack specifically the gene for endothelial nitric oxide synthase with their respective wild-type controls. (ahajournals.org)
  • specifically
  • First, with the use of knockout mice in which the eNOS isoform was specifically mutated, 14 15 we investigated whether NO that is generated by the endothelial isoform of NO synthase is involved in the blood pressure-buffering effect of NO. Second, with the use of power spectral analysis, we determined the frequency range in which endothelium-derived NO can buffer blood pressure variability in mice. (ahajournals.org)
  • blood
  • One day after carotid artery cannulation, blood pressure was recorded in these conscious mice. (ahajournals.org)
  • In addition, endothelial nitric oxide is most effective in buffering blood pressure oscillations at frequencies that range from 0.05 to 0.40 s −1 (Hz) in conscious mice. (ahajournals.org)