Knockout beta catenin. Medical search. Frequent questions

Ultrastructural analysis revealed that overexpression of CD97 strengthened lateral cell-cell contacts between enterocytes, which, in contrast, were weakened in CD97 knockout (Ko) mice. (nih.gov)
2002. Response of Apc(min) and A33 (delta N beta-cat) mutant mice to treatment with tea, sulindac, and 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP). . (oregonstate.edu)
Wnt/β-Catenin Signaling Induces Integrin α4β1 in T Cells and Promotes a Progressive Neuroinflammatory Disease in Mice. (ox.ac.uk)
In this article, we report that abnormal β-catenin expression in T cells drives a fatal neuroinflammatory disease in mice that is characterized by CNS infiltration of T cells, glial activation, and progressive loss of motor function. (ox.ac.uk)
Importantly, expression of β-catenin in mature naive T cells was sufficient to drive integrin α4β1 expression and CNS migration, whereas pharmacologic inhibition of integrin α4β1 reduced the abnormal T cell presence in the CNS of β-catenin-expressing mice. (ox.ac.uk)
Knockout mice implicate ROR alpha as being essential in the development of the cerebellum. (novusbio.com)

Interestingly, beta-catenin inhibited p53 transcriptional activity in SMCs in culture and in vivo, as concurrent loss of p53 improved arterial morphogenesis and embryonic development. (yu.edu)

The membrane was probed with a beta-Catenin monoclonal antibody (Product # MA1-301) at a dilution of 1:1000 overnight at 4 C on a rocking platform, washed in TBS-0.1%Tween 20, and probed with a goat anti-mouse IgG-HRP secondary antibody (Product # 31430) at a dilution of 1:20,000 for at least 1 hour. (labome.cn)
Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature and probed with a Catenin beta monoclonal antibody (Product # MA1-301) at a dilution of 1:200 or without primary antibody (negative control) overnight at 4 C in a humidified chamber. (labome.cn)

The Wnt pathway activates target genes by controlling the β-catenin-T-cell factor (TCF) transcriptional complex during embryonic development and cancer. (xenbase.org)

CD97 overexpression upregulated membrane-bound but not cytoplasmic or nuclear beta-catenin and reduced phospho-beta-catenin, labeled for degradation. (nih.gov)
In the lack of Wnt signaling, cytoplasmic -catenin is definitely destined by damage complicated healthy proteins, including AXIN, adenomatous polyposis coli (APC), and glycogen synthase kinase 3 beta (GSK3M). (insulin-receptor.info)

The knockout mouse is a mouse in which the gene is inactivated in order to study the role of genes. (wikipedia.org)
We show that inhibition of E-cadherin-mediated cell-cell contact in ES cells using gene knockout (Ecad(-/-)), RNA interference (EcadRNAi), or a transhomodimerization-inhibiting peptide (CHAVC) results in cellular proliferation and maintenance of an undifferentiated phenotype in fetal bovine serum-supplemented medium in the absence of leukemia inhibitory factor (LIF). (ox.ac.uk)
Here we report that inhibition of GSK3b increases nuclear translocation of b-Catenin, which forms a complex with TCF/LEF-1 to boost miR-183-96-182 cluster gene expression in gastric cancer cells. (gpr120inhibitor.com)
Our operate identifies miR-183-96-182 cluster gene as a downstream target regulated by b-Catenin/TCF/LEF-1 pathway in gastric cancer cells. (gpr120inhibitor.com)
Catenin can after that translocate to the nucleus, where it things with T-cell element/lymphoid booster element (TCF/LEF) transcription elements to promote focus on gene transcription (16). (insulin-receptor.info)
We and others have synthesized light-activatable cMOs that allow spatiotemporal control of RNA splicing or translation, complementing the use of conditional knockouts to study stage- and tissue-specific differences in gene function. (cdc.gov)
Significant downregulation of beta-defensin1 gene (DEFB1) expression was observed when human bronchial epithelial cells (HBEpCs) were exposed to IAV. (cdc.gov)

During blast crisis of chronic myelogenous leukemia (CML), abnormal granulocyte macrophage progenitors (GMP) with nuclear beta-catenin acquire self-renewal potential and may function as leukemic stem cells (Jamieson et al. (ca.gov)
To develop a mouse model for CML-initiating GMP, we expressed p210(BCR-ABL) in an established line of E2A-knockout mouse BM cells that retain pluripotency in ex vivo culture. (ca.gov)
These results provide further evidence that BCR-ABL-transformed GMP with abnormal beta-catenin activity can function as leukemic stem cells. (ca.gov)
We recently found that isolated HCC cells using a cell surface marker EpCAM (CD326) from alpha-fetoprotein (AFP) positive HCC cases are hepatic cancer stem cells (HepCSCs), where an activation of Wnt/β-catenin signaling is a major feature [ 5 ]. (biomedcentral.com)
We show that enhanced β-catenin expression in T cells leads to aberrant and Th1-biased T cell activation, enhanced expression of integrin α4β1, and infiltration of activated T cells into the spinal cord, without affecting regulatory T cell function. (ox.ac.uk)
Altogether, our developmental and adult mouse studies show that beta-catenin signaling function, especially that mediated by its C-terminal domain, promotes proliferation and survival of vascular SMCs in vivo, in part by repressing p53 activity in these cells. (yu.edu)
β-Catenin Knockdown Affects Mitochondrial Biogenesis and Lipid Metabolism in Breast Cancer Cells. (unich.it)
We used a label free analysis to compare trypsin-digested proteins from CTR (shCTR) and beta-catenin knockout cells (sh beta cat). (unich.it)
A loss of mitochondrial mass and membrane potential was also assessed by fluorescent probes in sh beta cat cells with respect to the controls. (unich.it)
These data are consistent with the reduced expression of transcriptional factors regulating mitochondrial biogenesis detected in sh beta cat cells. (unich.it)
Compared to controls, beta-catenin knockout cells showed increased incorporation of [1-C-14] acetate and decreased utilization of [U-C-14] glucose for fatty acid synthesis. (unich.it)
Our data highlight a role of beta-catenin in the regulation of metabolism and energy homeostasis in breast cancer cells. (unich.it)
Expression is particularly high in the pancreas where it is thought to play a role in the differentiation of pancreatic beta cells. (embl.de)
Although reversal of the LIF-independent phenotype in Ecad(-/-) ES cells is dependent on the beta-catenin binding domain of E-cadherin, we show that beta-catenin null (betacat(-/-)) ES cells also remain undifferentiated in the absence of LIF. (ox.ac.uk)
This suggests that LIF-independent self-renewal of Ecad(-/-) ES cells is unlikely to be via beta-catenin signaling. (ox.ac.uk)
To confirm the role of transforming growth factor beta family signaling in the self-renewal of Ecad(-/-) ES cells, we show that these cells maintain an undifferentiated phenotype when cultured in serum-free medium supplemented with Activin A and Nodal, with fibroblast growth factor 2 required for cellular proliferation. (ox.ac.uk)
Furthermore, -catenin may lead to telomerase upregulation in come and tumor cells by straight controlling TERT appearance via joining to the TERT marketer in complicated with Klf4, as previously reported in a research of mouse adult come cells and human being carcinoma lines NTera2 and SW480 (15). (insulin-receptor.info)
download complexes in NF1 occur derived extruded both in influence beta-cells like complex 1 and in a domain of possibly being products. (erik-mill.de)
Signaling by TGF-beta neoplasia neurogenesis can highly inhibit recruited by localization cells in SMAD2 and SMAD3( Fleming et al. (erik-mill.de)
Mature B cells do IgM and IgD events which play purchased at the adhesion injury with Ig-alpha( CD79A, MB-1) and Ig-beta( CD79B, B29) to refer the B substrate migration( BCR)( Fu et al. (erik-mill.de)
inhibiting of download to the translesion is cell of cartilage dependent PLP members( ITAMs) in the Standard cells of Ig-alpha and Ig-beta by Src phophorylate energy pathways, missing LYN, FYN, and BLK( Nel et al. (erik-mill.de)
In conclusion, the reduction in IAV copy number in DEFB1 overexpressing cells suggests that beta-defensin-1 plays a key role in regulating IAV survival through STAT3 and is a potential target for antiviral drug development. (cdc.gov)

The abstract states, "Glutathione-S-transferase (GST) pulldown assays were used to show that RPTPrho interacts with several adherens junctional proteins in brain, including E-cadherin, N-cadherin, VE-cadherin (cadherin-5), desmoglein, alpha, beta [Ctnnb1] and gamma [Jup] catenin, p120(ctn) and alpha-actinin. (geneontology.org)
Mouse Ctnnb1: Is the GO:0045453 "bone resorption" annotation from PMID 15866165 correct, or is it the phenotype of a beta catenin knockout? (geneontology.org)
Homo sapiens catenin beta 1 (CTNNB1), transcript variant 1, mRNA. (origene.com)

We hypothesized that Wnt/β-catenin signaling transcriptionally activates microRNA-181s in HCC. (biomedcentral.com)
Further experiments confirmed that the expression of ATDC triggers beta-catenin, a cell-signaling protein that, upon receiving the right trigger, activates genes including SOX9 . (nyulangone.org)
Inhibition of GSK3b activates miR-181 expression by means of Wnt/beta-catenin signaling in HCC (34). (gpr120inhibitor.com)

Targeted knockout of BRG1 potentiates lung cancer development. (igbmc.fr)
Telomerase maintains telomeres canonically, but latest reviews possess suggested that the primary proteins mammalian telomerase change transcriptase (TERT) element, together with the chromatin remodeling element BRG1 and -catenin, might also situation to and promote appearance of Wnt focus on genetics. (insulin-receptor.info)
Those writers additionally reported protein-protein relationships between hTERT and the chromatin redesigning element BRG1 and between hTERT and -catenin. (insulin-receptor.info)

MiR-26a promotes cholangiocarcinoma by way of reducing GSK3b expression, resulting in b-Catenin activation (35). (gpr120inhibitor.com)
Primary antibodies and primers GSK3b (3D10) mouse mAb, Lef-1 (C12A5) rabbit mAb, b-Catenin (6B3) rabbit mAb, CK1e Factor Xa list polyclonal antibody, CK2a polyclonal antibody, FoxO1 rabbit mAb and b-Catenin (L87A12) mouse mAb were purchased from Cell Signaling Technology. (gpr120inhibitor.com)

2006. Tumors from rats given 1,2-dimethylhydrazine plus chlorophyllin or indole-3-carbinol contain transcriptional changes in beta-catenin that are independent of beta-catenin mutation status. . (oregonstate.edu)

Further, the use of these knockout models determined the significance of Tbx2 in the BMP signaling pathway for development of the atrioventricular canal, atrioventricular nodal phenotype, and atrioventricular cushion. (wikipedia.org)

Cutaneous cancer stem cell maintenance is dependent on beta-catenin signalling. (igbmc.fr)
For this dual role, different classes of proteins are differentially regulated via beta-catenin dependent mechanisms. (unich.it)
Her work was focused on the understanding of the nutrient-sensing O-GlcNAcylation in colorectal cancer development with a special interest in diet-dependent modification of the oncogene beta-catenin. (mcw.edu)
She also developed a brain O-GlcNAcase knockout model and studied the impact of sugar consumption during pregnancy on O-GlcNAc-dependent development of metabolic homeostasis. (mcw.edu)

Recently, alpha/beta-hydrolase domain-containing (ABHD) proteins have emerged as novel pivotal regulators in lipid synthesis or degradation while their functions in ESCs have not been investigated. (inra.fr)
Here, we applied a liquid chromatography-mass spectrometry (LC-MS/MS) approach to identify proteins modulated after beta-catenin knockdown in the breast cancer cell line MCF-7. (unich.it)
Loss of beta-catenin induced morphological changes and a significant modulation of the expression levels of proteins associated with primary metabolic processes. (unich.it)
In detail, proteins involved in carbohydrate metabolism and tricarboxylic acid cycle were found to be down-regulated, whereas proteins associated to lipid metabolism were found up-regulated in sh beta cat compared to shCTR. (unich.it)
These amyloid plaques are immunoreactive with antibodies to the prion protein and do not immunoreact with antibodies to other amyloidogenic proteins, such as the amyloid-beta (which is deposited in Alzheimer disease). (medscape.com)

2003. Suppression of tumorigenesis in the Apc(min) mouse: down-regulation of beta-catenin signaling by a combination of tea plus sulindac. . (oregonstate.edu)
2008. Protective versus promotional effects of white tea and caffeine on PhIP-induced tumorigenesis and beta-catenin expression in the rat. . (oregonstate.edu)

Together, these results implicate deregulation of the Wnt/β-catenin pathway in CNS inflammation and suggest novel therapeutic strategies for neuroinflammatory disorders. (ox.ac.uk)

Finally, Abhd11 knockout led to the misexpression of key metabolic enzymes related to lipid synthesis, glycolysis, and amino acid metabolism, and ABHD11 contributed to the homeostasis of lipid metabolism. (inra.fr)
beta-catenin plays an important role as regulatory hub in several cellular processes including cell adhesion, metabolism, and epithelial mesenchymal transition. (unich.it)

2003. Promotion versus suppression of rat colon carcinogenesis by chlorophyllin and chlorophyll: modulation of apoptosis, cell proliferation, and beta-catenin/Tcf signaling. . (oregonstate.edu)

EpCAM + AFP + HepCSCs have an activated Wnt/β-catenin signaling with a parallel increased expression of all four microRNA-181 family members. (biomedcentral.com)
Using both western blot and quantitative reverse transcriptase-PCR analyses, we found that the expression of all four microRNA-181 family members was positively correlated with β-catenin expression in HCC cell lines. (biomedcentral.com)
MicroRNA-181 expression could be directly induced upon an activation of Wnt/β-catenin signaling, which includes Wnt10B overexpression, inhibition of GSK3β signaling by LiCl, or forced expression of β-catenin/Tcf4. (biomedcentral.com)
To test whether miR-181s were transcriptional targets of Wnt/β-catenin, we first examined the expression of β-catenin and miR-181s in five different HCC cell lines. (biomedcentral.com)
We found that beta-catenin expression was undetectable in SMCs of normal arteries. (yu.edu)
beta-catenin driven metabolic reprogramming resulted also in a significant modulation of lipogenic enzyme expression and activity. (unich.it)

In addition, we demonstrate that this family is critical in maintaining stemness of EpCAM + AFP + HepCSCs, in part by directly targeting an inhibitor of Wnt/β-catenin signaling (nemo-like kinase [NLK]) and two hepatic transcriptional regulators of differentiation, i.e., caudal type homeobox transcription factor 2 (CDX2) and GATA binding protein 6 (GATA6). (biomedcentral.com)

Knockout of Abhd11 hampered ESC expansion and differentiation, triggering the autophagic flux and apoptosis. (inra.fr)
In these vessels, beta-catenin function was dispensable to maintain their structure and state of SMC differentiation. (yu.edu)

The antimicrobial peptide, beta-defensin-1, has antiviral activity against both enveloped and non-enveloped viruses. (cdc.gov)

Tbx2 has shown septal defects of the outflow tract, and this has been shown using a knockout mouse. (wikipedia.org)
The development of a Tbx2 knockout mouse model allowed for the determination of specific roles of Tbx2 in cardiac development, and scientists determined Tbx2 and Tbx3 to be redundant in much of heart development. (wikipedia.org)
Detection of Human/Mouse/Rat ss-Catenin antibody by Western Blot. (labome.cn)
The leukemogenic GMP displayed higher levels of beta-catenin activity than either the nontransformed GMP or the transformed nonGMP, both in culture and in transplanted mouse BM. (ca.gov)
We found that complete loss of beta-catenin in SMCs in the mouse decreased their proliferation and survival, limited arterial wall formation, and caused embryonic lethality. (yu.edu)

Using a series of beta-catenin knock-in mutant alleles, we found further that SMC beta-catenin signaling function was essential, while its structural role was not sufficient. (yu.edu)
To characterize SMC beta-catenin function in adulthood, we employed a tamoxifen-inducible SMC-specific beta-catenin knockout strategy. (yu.edu)

When secreted Wnt protein situation to Frizzled and low-density lipoprotein receptor-related protein (LRPs) at the plasma membrane layer, a sign is definitely transduced to destabilize the -catenin damage complicated. (insulin-receptor.info)
The replication of prions involves the recruitment of the normally expressed prion protein, which has mainly an alpha-helical structure, into a disease-specific conformation that is rich in beta-sheet. (medscape.com)

2017. Divergent roles of p120-catenin isoforms linked to altered cell viability, proliferation, and invasiveness in carcinogen-induced rat skin tumors. . (oregonstate.edu)

Thus, disrupting beta-catenin C-terminal interactions is an interesting potential therapeutic strategy for cardiovascular diseases associated with intimal hyperplasia such as atherosclerosis and restenosis. (yu.edu)

Although beta-catenin provides essential structural and signaling functions during development and adult homeostasis, its contributions to in vivo SMC activities in vascular assembly and remodeling have not been characterized. (yu.edu)
We hypothesized that beta-catenin is an essential modulator of vascular SMC biology in vivo. (yu.edu)
We will investigate how LGR5 knockout (KO) compared to elimination of LGR5-positive CSCs affects tumor growth, metastasis, drug resistance, and cancer cell signaling using patient-derived xenograft (PDX) organoids models ex vivo and in vivo. (grantome.com)

The team found it to be more active in human ADM lesions along with beta-catenin and SOX9 , and its activation increased further during the transition of ADM into human pancreatic ductal adenocarcinoma. (nyulangone.org)
The absence turn SYK is the reactive activation such acids( ITAMs) on the extrahepatic substrates of Ig-alpha( CD79A, MB-1) and Ig-beta( CD79B, B29)( Wienands et al. (erik-mill.de)

Specifically, the beta-catenin C-terminal interaction domain was required and sufficient to provide the necessary signal for arteriogenesis, whereas the N-terminal interaction domain was dispensable. (yu.edu)

Signaling by the TGF-beta biosynthesis repeat is content floccular, as it binds spectra step and stabilizes Downregulation energy and penetration( Shipley et al. (erik-mill.de)

Please check the GO:0045177 "apical part of cell" annotation from MGI:3037622/PMID 15037549, which states, "beta-catenin, f-actin, and myosin II-B localize predominantly to the apical-junctional complex in normal neuroepithelium (Fig. 6E-G)." Is there additional supporting evidence for the 45177 annotation? (geneontology.org)

and in this context, loss of beta-catenin in SMCs significantly reduced cell proliferation and survival in the neointima and limited neointimal growth. (yu.edu)

In this study, we identified that Wnt/β-catenin signaling appears to be a common activator to induce miR-181s. (biomedcentral.com)

Therefore, beta-catenin serves as an essential repressor of p53 in SMCs during arterial wall formation in the systemic circulation. (yu.edu)
We reported that LGR5 binds R-spondin growth factors and functions to potentiate Wnt/beta-catenin signaling, an essential pathway that is activated in CSCs. (grantome.com)

Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1. (abcam.com)

Ovarian AR was not influenced by either treatment, and oviduct AR was reduced after ethanol-melatonin combination: Oviduct ER-alpha, ER-beta and uterine ER-beta were downregulated by either ethanol or melatonin. (liverxreceptor.com)

In rheumatoid gut, passaging by TGF-beta may be blood linking, as it results viral process( EMT), extracellularly according development( Cui et al. (erik-mill.de)

Anatomy11

Organisms3

Diseases7

Chemicals and Drugs50

Analytical, Diagnostic and Therapeutic Techniques and Equipment13

Phenomena and Processes18

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Information Science3

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Monoclonal antibody2

Embryonic development1

Cytoplasmic2

Gene7

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BRG13

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Dependent4

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Tumorigenesis2

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Carcinogenesis1

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