EnzymeEnzymaticDeterminationPROTEINSCharacterizationVitroTotal antioxidantEquilibriumParametersAntioxidantOptimisationReactionSuperoxideDetectionProcessesSensitivityVivoChemicalMacromoleculesAnalytical chemistryQuantitativelyTargetsMoleculesFunctional1993MethodsInteractionsMethodAffinityBiologyDepartmentLaboratoryEnhancementCompoundsPhysicalDrugRelevanceRatePharmaceutical1986ExperimentalSystemsDataDesignJournalTechniquesDevelopmentModelTargetLeadStandardInterestDevelop
Enzyme4
- The double reciprocal plot distorts the error structure of the data, and is therefore not the most accurate tool for the determination of enzyme kinetic parameters. (wikipedia.org)
- Standard protein purification and characterization techniques, enzyme activity assays, as well as immunoassays (ELISA) will also be taught. (uaeu.ac.ae)
- A simplified kinetic model was used to fit the experimental data, in which specific activities in Cellic CTec3 were not differentiated, enzyme adsorption was ignored, and end-product inhibition was only attributed to glucose accumulation. (ncsu.edu)
- The adjusted kinetic model was used to predict the effects of substrate and enzyme intermittent feedings in fed-batch hydrolysis experiments. (ncsu.edu)
Enzymatic2
- We cater for the chemical and biotech industries, as well the agri-food and health sectors, with a view to: validating an enzymatic assay method, improving the physico-chemical and rheological properties of an industrial product, and creating a functioning extraction process. (capacites.fr)
- ref name="Art4">Reference Range and Method Comparison Studies for Enzymatic and Jaffé Creatinine Assays in Plasma and Serum and Early Morning Urine, Mazzachi BC, Peake MJ, Ehrhardt V., Clin Lab 2000;46:53-55. (sensus.org)
Determination2
- A literature survey revealed a spectrophotometric 7 and few High Performance Thin Layer Chromatographic Method (HPLTC) for Manidipine Dihydrochloride determination in human biological fluids 8, 9 . (ijpsr.com)
- However no high performance liquid chromatographic methods were found for Manidipine determination in bulk drug and formulations as a stability indicating assay method. (ijpsr.com)
PROTEINS3
- This course will cover the structure and function of different biological molecules, including proteins, carbohydrates, lipids and nucleic acids. (uaeu.ac.ae)
- Biomolecular interaction analysis, especially SPR, is used increasingly in drug development to characterise the affinity of binding between biological targets (e.g. proteins or nucleic acids) and drugs, antibodies and antigens or any series of interacting molecules. (bioascent.com)
- Both Anna and Malin will mainly concentrate on the study of the physical chemistry of proteins. (lu.se)
Characterization1
- In small-molecule lead characterization, label-free assays are playing a pivotal role in validating the direct binding of small molecule and fragments to target protein. (drugdiscoverynews.com)
Vitro1
Total antioxidant2
- It is therefore important to be able to quantitatively assess the total antioxidant power or capacity within biological specimens. (spagades.com)
- The total antioxidant capacity was assayed by ferric reducing ability of plasma (FRAP), phosphomolybdenum antioxidant power (PAP), reduction assays. (degruyter.com)
Equilibrium3
- This provides a powerful technique for monitoring real time interactions and establishing the kinetics of ligand binding - association and dissociation rates (ka, kd), and the equilibrium dissociation constant (KD). (bioascent.com)
- This information can be used to drive chemistry optimisation efforts in drug development or rationalise or model the behaviour of drugs in complex non-equilibrium in vivo settings. (bioascent.com)
- Creatinine is usually produced at a fairly constant rate so that its equilibrium concentration is fairly constant in healthy individuals. (sensus.org)
Parameters4
- While the Lineweaver-Burk plot has historically been used for evaluation of the parameters, together with the alternative linear forms of the Michaelis-Menten equation such as the Hanes-Woolf plot or Eadie-Hofstee plot, all linearized forms of the Michaelis-Menten equation should be avoided to calculate the kinetic parameters. (wikipedia.org)
- The Lineweaver-Burk plot derives from a transformation of the Michaelis-Menten equation, v = V a K m + a {\displaystyle v={\frac {Va}{K_{\mathrm {m} }+a}}} in which the rate v {\displaystyle v} is a function of the substrate concentration a {\displaystyle a} and two parameters V {\displaystyle V} , the limiting rate, and K m {\displaystyle K_{\mathrm {m} }} , the Michaelis constant. (wikipedia.org)
- The harvested prokaryotic tat-GFP fusion protein will serve as an assay, allowing us to determine the kinetic parameters for our system. (igem.org)
- The kinetic parameters, such as transfer co-efficient, diffusion co-efficient, and heterogeneous forward rate constant values are evaluated by employing these techniques. (ijpsr.com)
Antioxidant1
- Flavonoids possess different interesting biological properties, including antibacterial, antiviral, anti-inflammatory and antioxidant activities. (mdpi.com)
Optimisation2
- employ SPR (or alternative biophysical techniques) as a primary or orthogonal assay to drive design make test cycles in hits-to-lead and lead optimisation. (bioascent.com)
- With over 15 years working in drug discovery Stuart has extensive experience of developing and trouble-shooting novel screening assays, designing screening cascades, compound screening, hit validation and supporting hit to lead and lead optimisation programs. (bioascent.com)
Reaction3
- By rapidly mixing apo M ferritin from frog, Fe(2+), and O(2) and allowing the reaction to proceed for 70 ms when F(peroxo) has reached its maximum accumulation, followed by spraying the reaction mixture into the H(2)O(2) assay solution, we were able to quantitatively determine the amount of H(2)O(2) produced during the decay of F(peroxo). (unl.pt)
- The Sandell-Kolthoff reaction is based on the Ce 4+ -As 3+ iodine catalyses redox reaction, with rate constant that is stable over the first 3 minutes. (iprojectdownload.com)
- Xiashi and Yiaqin(2008), satisfactorily applied inhibition kinetic method, which was based on iodine inhibition effects on the reaction between rhodamine and KBrO 3 in H 2 SO 4 medium at 45±0.5 O C for 15 min to determine iodide concentration in kelp. (iprojectdownload.com)
Superoxide2
- This review aims to describe the structural characteristics of superoxide reductases (SORs) and mechanistic aspects of biological superoxide anion reduction. (unl.pt)
- A number of endogenous systems, such as the aerobic metabolism and electron transport chains, generate highly reactive molecules with important biological functions known as reactive oxygen species (ROS), including superoxide and hydrogen peroxide (H2O2). (bvsalud.org)
Detection3
- Label-free detection, on the other hand, is proving to be a highly sensitive method of measurement for endogenous targets in live cell assays, and eliminates the need for tags, dyes or specialized reagents or engineered cells-reducing the resources required for assay development, simplifying assay design, minimizing liabilities created by the use of labels and enabling the use of native cells for greater biological relevance. (drugdiscoverynews.com)
- This methodology enables detection of putative metabolites from biological samples and simultaneous quantification of the pattern and extent of isotope labeling. (acs.org)
- Lab-on-a-chip-based PCR-RFLP assay for the confirmed detection of short-length feline DNA in food. (uinsgd.ac.id)
Processes3
- The focus of McCarty's research, teaching, and writing has been on water, with a primary interest in biological processes for the control of environmental contamination. (stanford.edu)
- His early research was on anaerobic treatment processes, biological processes for nitrogen removal and water reuse. (stanford.edu)
- In the search for life elsewhere in the solar system, we have some expectations about what life might look like: It will likely involve carbon chemistry, and a spatial segregation of organic compounds in a way that simple chemical processes would not permit. (nationalacademies.org)
Sensitivity1
- These methods are often at the end of sophistication, requiring expensive instrumentation with qualified personnel (inductively coupled plasma-mass spectrometry, instrumental neutron activation analysis) or by kinetic colorimetric method requiring oxidative sample digestion (Dasgupta and Shelor, 2011).The kinetic method has many merits, for example, high sensitivity, good selectivity and simple experimental procedures as well as good reproducibility and low budget apparatus. (iprojectdownload.com)
Vivo1
- In November 2010, we brought you the news that ACEA Biosciences Inc. and Vivo Biosciences Inc. agreed to work together to develop label-free and real-time cell-based assays for the xCELLigence System, which is co-developed by Roche and ACEA and marketed by Roche Applied Science. (drugdiscoverynews.com)
Chemical5
- In: Threshold limit values for chemical substances and physical agents and biological exposure indices. (cdc.gov)
- We describe the Framework for Integrated, Conceptual, and Systematic Microbial Ecology (FICSME), an experimental design framework for conducting process-focused microbial ecology studies that incorporates biological, chemical, and physical drivers of a microbial system into a conceptual model. (frontiersin.org)
- Composting is a biological and chemical process to convert degradable organic matter into stable humus, which is a kind of continuous and efficient organic waste treatment technology. (scirp.org)
- Lignin is an aromatic polyether that provides plant cell wall rigidity, improves its mechanical strength, and protects cellulose and hemicelluloses from chemical and biological attack. (ncsu.edu)
- The Division of Surface Chemistry of the Swedish Chemical Society. (lu.se)
Macromolecules1
- Reactive oxygen species (ROS), products of normal cell metabolism may cause damage to biological macromolecules leading to severe health threats when they are present in high concentrations. (degruyter.com)
Analytical chemistry2
- Investigate advanced topics such as drug design, drug analysis and analytical chemistry. (hud.ac.uk)
- Studying for your Pharmaceutical Chemistry BSc(Hons) degree with us gives you the chance to explore all the fundamentals of organic chemistry, analytical chemistry, biochemistry and pharmacology while developing a specialism in pharmaceutics. (hud.ac.uk)
Quantitatively1
- During recent years, the biotic ligand model (BLM) has been proposed as a tool to evaluate quantitatively the manner in which water chemistry affects the speciation and biological availability of metals in aquatic systems. (researchgate.net)
Targets2
- better inform the selection of candidate molecules by understanding how their kinetic profile is likely to impact upon PK/PD relationships and selectivity over closely related anti-targets. (bioascent.com)
- Prior to this Stuart helped establish the Dundee Drug Discovery Unit (DDU) where he spent 7 years as a project and then team leader working across a wide array of novel drug targets and assays. (bioascent.com)
Molecules3
- Categorize Biological Membranes And Various Mechanisms Of Transport Of Molecules Through Membranes. (uaeu.ac.ae)
- In this area, we have developed a methodology for the incorporation of protected isocyanates into organic molecules and this chemistry is being applied to the synthesis of a diverse set of biologically important, nitrogen-containing heterocycles. (exeter.ac.uk)
- My teaching activities cover all aspects of organic chemistry from basic structure and bonding in organic molecules, to complex organic synthesis. (exeter.ac.uk)
Functional1
- In December 2009, we reported that Caliper Life Sciences entered into an agreement with SRU Biosystems to use its label-free BIND technology to offer new functional assays as part of its discovery alliances and services. (drugdiscoverynews.com)
19932
- 1993. A comparative study of the kinetic and bioavailability of pure and soil-adsorbed benzene, toluene, and m -xylene after dermal exposure. (cdc.gov)
- PCR was invented over 25 years ago by Kary Mullis [Saiki, 1985], for which he received the Nobel Prize in chemistry in 1993 [Malmström, 1997]. (gene-quantification.org)
Methods1
- This obvious lack of standardization at every step of the assay ( Figure 1 ) is exacerbated by significant differences in sample processing, use of controls, normalization methods and quality control management and has serious implications for the reliability, relevance and reproducibility of RT-qPCR. (gene-quantification.org)
Interactions1
- As advances continue to be made in screening technologies, the use of labels in traditional drug discovery screening assays has been criticized as producing undesirable and unanticipated interactions that can compromise screening data and lead to false conclusions. (drugdiscoverynews.com)
Method2
- This method makes it possible to detect specific mRNA transcripts in any biological sample. (gene-quantification.org)
- To this end, a horseradish peroxidase-catalyzed spectrophotometric method was used as an assay for H(2)O(2). (unl.pt)
Affinity1
- Also, labeled assays show end-point results only and do not allow real-time kinetic measurements such on-rates, off-rates and affinity measurements, which lead to critical information being missed, or weak binding compounds being missed in a screen. (drugdiscoverynews.com)
Biology2
- 1974. Biological handbooks: Biology data book. (cdc.gov)
- the understanding of MSC biology, their mechanism of action (MoA) in different biological contexts, and their targeted and routine use in the clinics is limited ( Hoogduijn and Lombardo, 2019 ). (frontiersin.org)
Department2
- Anglo Platinum Research Centre, the Nelson Mandela Metropolitan University and the Inorganic Chemistry Department for financial assistance. (studyres.com)
- Ulf Olsson takes a position in the steering group of the Department of Chemistry. (lu.se)
Laboratory1
- The course is offered to students who have a solid chemistry background, good laboratory skills and have an interest in scientific research. (uaeu.ac.ae)
Enhancement1
- Clonogenic and cell viability assays were employed to determine cells survival curves from which the dose enhancement levels "radiosensitisation" are deduced. (medsci.org)
Compounds2
- The next steps for the control of witches' broom plague involve the synthesis and biological evaluation of these compounds, which are in progress. (biomedcentral.com)
- Visible-light microscopes can be used to obtain some information about the chemistry of nonfluorescing compounds at a spatial resolution of 200 to 1000 nm. (nationalacademies.org)
Physical2
- This is a big boost for the science at the Division of Physical Chemistry. (lu.se)
- A sign of immediate international recognition of the new Physical Chemistry team is that the Division has already been selected as a partner, with responsibilities in light scattering and nuclear magnetic resonance, in the EU-sponsored infrastructure in soft matter (see p. 29 for details). (lu.se)
Drug1
- In April 2010, we reported that ForteBio chose Tecan as its preferred automation partner for real-time, label-free assays to support bioprocess and drug discovery workflows. (drugdiscoverynews.com)
Relevance1
- accordingly, the relevance of the findings of these assays in vivoto systems is uncertain [8]. (spagades.com)
Rate1
- Thus, a rate difference in a single kinetic step accounts for specificity. (elifesciences.org)
Pharmaceutical1
19861
- 1986. Documentation of the threshold limit values and biological exposure indices. (cdc.gov)
Experimental4
- Such an approach is characterized by an iterative process, including biological data acquisition and integration, network construction, mathematical modeling and experimental validation. (hindawi.com)
- However, experimental approaches have limitations when dealing with complex biological systems composed of multiple layers of regulation such as the transcriptional and post-transcriptional regulation by transcription factors (TFs) and miRNAs [ 2 ]. (hindawi.com)
- depending on the biological problem investigated and experimental data available, the interaction map can be translated into a detailed mechanistic model that can simulate the temporal evolution of molecular entities. (hindawi.com)
- 3 Chapter 2 Figure 2.1: Illustration of the experimental system employed to record UV-Vis spectra at constant temperatures Figure 2.2: 11 Illustration of the experimental setup used during the preparation of a pure OsO4 solution Figure 2.3: 18 UV-Vis spectra illustrating the formation of the [Os(NH2CSNH2)6] 3+ species as a function of thiourea concentration. (studyres.com)
Systems1
- The need to understand the molecular mechanisms in species has made the PCR an indispensable tool for understanding the functioning of these biological systems. (intechopen.com)
Data3
- Knowledge of the enzyme's structure is helpful in interpreting kinetic data. (wikipedia.org)
- The significant problems caused by variability of RNA templates, assay designs and protocols, as well as inappropriate data normalization and inconsistent data analysis, are widely known but also widely disregarded. (gene-quantification.org)
- The study of biological complexity is a new frontier that requires high-throughput molecular technology, high speed computer memory, new approaches to data analysis, and the integration of interdisciplinary skills. (intechopen.com)
Design1
- model predictions together with biological explanations are integrated to guide the design of new experiments, which in turn validate or falsify the model. (hindawi.com)
Journal4
Techniques1
- employ SPR (or alternative biophysical techniques) as an orthogonal assay in hit confirmation and characterisation to confirm direct target engagement. (bioascent.com)
Development2
- Early developments in the aquatic chemistry of metals, the physiology of aquatic organisms and aquatic toxicology are reviewed first, and the degree to which each of these disciplines influenced the development of water quality regulations is discussed. (researchgate.net)
- We conclude that further development of targeted approaches should prevent inaccurate conclusions about the nature of biological regulations and in particular kinase-substrate networks. (acs.org)
Model1
- If model predictions are in agreement with the experiments, the model justifies the biological hypotheses behind it. (hindawi.com)
Target1
- screen low molecular weight fragment libraries (including our in-house fragment library) against your biological target of interest. (bioascent.com)
Lead1
- In turn, these hypotheses, which provide reasonable explanations for the biological phenomenon, lead to an enhanced understanding of the gene regulatory network. (hindawi.com)
Standard1
- Although it is often described as a ''gold'' standard, it is far from being a standard assay. (gene-quantification.org)
Interest1
- The microscopic investigation of possibly life-containing specimens can be greatly aided by looking not just at the morphology of a feature of interest, but at its chemistry as well. (nationalacademies.org)
Develop3
- Our experts in these fields develop powerful molecular tools for applications in agri-food, health, nutraceuticals, cosmetics, biotechnology and green chemistry. (capacites.fr)
- The expertise in the team, combined with the capabilities of these instruments, allows us to develop and transfer a wide array of assays. (bioascent.com)
- Thus, toxicity assessment, increasing the predictive power and scalability of there is a need to develop a more complete understanding of such assays. (cdc.gov)