• Osteocytes compose lacunar-canalicular network by their cytoplasmic dendrites that connect them to each other and to cells on the bone surface, important for intracellular and extracellular communication (Figure 1 ) [ 11 , 12 ]. (biomedcentral.com)
  • Osteocytes osteocalcin express, galectin 3, Compact disc44, and many other bone tissue matrix proteins that support intercellular adhesion and regulate exchange of nutrient in the bone tissue liquid within lacunae as well as the canalicular network. (nostradamus2018.com)
  • This osteocyte lacuno-canalicular network (OLCN) is believed to play important roles in mechanosensing, mineral homeostasis, and for the mechanical properties of bone. (uni-wuerzburg.de)
  • On the level of statistical topological properties (edges per node, edge length and degree distribution), both network types are indistinguishable, highlighting that despite pronounced differences at the tissue level, the topological architecture of the osteocyte canalicular network at the subcellular level may be independent of species and bone type. (uni-wuerzburg.de)
  • Osteocytes do not entirely fill up the canaliculi. (wikipedia.org)
  • Materials picked up by osteocytes adjacent to blood vessels are distributed throughout the bone matrix via the canaliculi. (wikipedia.org)
  • Osteocytes possess long dendrite-like procedures increasing throughout canaliculi (tunnels) inside the mineralized matrix. (nostradamus2018.com)
  • And, we furtherly performed single-cell spatial transcriptomic sequencing on postnatal day 1 mouse enthesis, in order to deconvolute bone-tendon junction (BTJ) chondrocytes onto spatial spots. (elifesciences.org)
  • Effect of hypertrophic differentiation of chondrocytes on remodelling of the osteochondral junction. (transimmunom.fr)
  • The radiating processes of the osteocytes (called filopodia) project into these canals. (wikipedia.org)
  • These cytoplasmic processes are joined together by gap junctions. (wikipedia.org)
  • Moreover, increasing number of studies have highlighted the fact that a multifaceted approach from various domains of science such as biomechanics, cell biology, bioengineering, biophysics, biomaterials, computational modelling, endocrinology, and orthopaedics is essential to further our understanding of the intricate processes involved in bone remodelling and the central role of osteocytes in maintaining bone mass and architecture. (pharmaceuticalintelligence.com)
  • They have cytoplasmic processes that extend through the bone matrix and are connected to each other through gap junctions. (relationshipbetween.com)
  • Local bone gain and loss--as well as remodeling in response to fatigue damage--[are] processes supervised by mechanosensitive osteocytes. (heightquest.com)
  • Osteocytes and their cell processes reside in a large, interconnected network of voids pervading the mineralized bone matrix of most vertebrates. (uni-wuerzburg.de)
  • In addition to providing microenvironmental support for hematopoietic processes, BM-MSCs can differentiate into various mesodermal lineages including osteoblast/osteocyte, chondrocyte, and adipocyte that are crucial for bone metabolism. (ijbs.com)
  • Several proteins involved in osteoblastic/osteocyte and osteoclastic differentiation and activity were studied in the three experimental groups: control (CO) group (wild type mice, Igf +/+ , n = 10), heterozygous Igf +/- group with partial IGF-I deficiency (Hz, n = 10), and heterozygous Igf +/- mice treated with IGF-I for 10 days (Hz + IGF-I, n = 10). (biomedcentral.com)
  • The principal function from the interaction between your osteocyte-osteoblast/coating cell syncytium is certainly mechanosensation [15]. (nostradamus2018.com)
  • Osteocytes are connected and electrically through distance junctions constructed mainly of connexin 43 metabolically, which are necessary for osteocyte maturation, function, and success [18]. (nostradamus2018.com)
  • Cx43 (E2) antibody, an antibody which particularly blocks Cx43 hemichannel activity however, not difference junctions completely obstructed dye uptake induced by H2O2 and additional exacerbated H2O2-induced osteocytic cell loss of life. (ppbhg.org)
  • 16). Hemichannels produced by Cx43 have already been shown to control the discharge of NAD+, prostaglandin E2 (PGE2) and ATP in response to mechanised arousal in osteocytes and mesenchymal Pinocembrin stem cells (17C20). (ppbhg.org)
  • As opposed to the result of hemichannels, Cx43 difference junction stations conferred security to individual retinal pigment epithelial cell series against the basal fluorescence. (ppbhg.org)
  • Outcomes Operating-system Induced Cell Loss of life and Reduced Cx43 Appearance in Osteocytic cells To elucidate the result of Operating-system on osteocytes, we treated MLO-Y4 cells with different dosages (0 C 0.5 mM) of H2O2, as well as the cell apoptosis and loss of life indicated by PI and annexin V staining, respectively, had been quantified by FACS analyses. (ppbhg.org)
  • Open up in another screen Fig. 1 H2O2 induces cell loss of life and lowers Cx43 appearance in osteocyte cell series. (ppbhg.org)
  • Gap junctions and hemichannels are the main routes of interactions in bone tissue. (biomedcentral.com)
  • Connexin (Cx) complexity: Cxs oligomerize to form hemichannels (connexons) that are transported to the cell surface where they dock with hemichannels from a contacting cell to form intercellular gap junction channels [ 1 ]. (biomedcentral.com)
  • Latest reports have got implicated the function of hemichannels and difference junctions in regulating susceptibility of cells to OS-induced cell loss of life. (ppbhg.org)
  • Neural input into this bioprinted skeletal muscle construct shows the improvement of myofiber formation, long-term survival, and neuromuscular junction formation in vitro. (nature.com)
  • However, recent developments in imaging and tissue processing have made it possible to study osteocytes in their natural milieu. (pharmaceuticalintelligence.com)
  • Perhaps, the levels of mechanical strain required to initiate these biological events are not perceived by the osteocytes embedded within the bone tissue. (pharmaceuticalintelligence.com)
  • Osteocytes transduce tension indicators from stretching out or twisting of bone tissue into biologic activity and react to mechanical fill. (nostradamus2018.com)
  • As a result, osteocytes start and direct the next remodeling support and procedure bone tissue framework and fat burning capacity. (nostradamus2018.com)
  • Dye transfer assay demonstrated that H2O2 decreased the difference junction intercellular conversation (GJIC). (ppbhg.org)
  • Intercellular mitochondrial transfer occurs in different ways, including tunnelling nanotubes (TNTs), extracellular vesicles (EVs), and gap junction channels (GJCs). (biomedcentral.com)
  • Osteocytes are the most abundant cell type in bone and are responsible for sensing mechanical strain and signaling bone (re)modeling, making them the primary mechanosensors within the bone. (pharmaceuticalintelligence.com)
  • Osteocytes are the most abundant type of bone cell. (relationshipbetween.com)
  • Gap junction channels are even more complex as connexin subunits can form homomeric or heteromeric arrangements that dock across the extracellular space to form homo- or heterotypic channels. (biomedcentral.com)
  • Ai XL, Chi Q, Qiu Y, Li HY, Li DJ, Wang JX, Wang ZY (2017) Gap junction protein connexin43 deregulation contributes to bladder carcinogenesis via targeting MAPK pathway. (edu.pl)
  • Banerjee D, Gakhar G, Madgwick D, Hurt A, Takemoto D, Nguyen TA (2010) A novel role of gap junction connexin46 protein to protect breast tumors from hypoxia. (edu.pl)
  • Channels typically cluster into crystalline structures known as gap junction plaques where they act to exchange numerous small molecules important in cell signalling [ 1 ]. (biomedcentral.com)
  • Osteocytes are the most common type of cell found in bone and are connected to other osteocytes, forming a network. (relationshipbetween.com)
  • Osteocytes are trapped in the bony matrix but may be released as a result of microfractures(which may possibly be caused by LSJL). (heightquest.com)
  • The attachment site of the rotator cuff (RC) is a classic fibrocartilaginous enthesis, which is the junction between bone and tendon with typical characteristics of a fibrocartilage transition zone. (elifesciences.org)
  • Osteocytes control phosphate matrix and fat burning capacity mineralization through the secretion of phosphate-regulating elements such as for example FGF23, Phex, Dmp1, and appearance of sclerostin (encoded by gene SOST) and DKK1 that adversely regulates Wnt and BMPs signaling [17]. (nostradamus2018.com)
  • The line of junction of the epiphysis and diaphysis of a long bone where growth in length occurs. (chezbebeny.com)