• This allows different daughter cells from the same activated B cell to produce antibodies of different isotypes or subtypes (e.g. (wikipedia.org)
  • Antibodies detected were all of the IgG isotype. (nih.gov)
  • IgA-deficient patients with immunoglobulin E (IgE)-class anti-IgA antibodies are at risk for anaphylaxis if they receive blood or intravenous immunoglobulin, but this situation is extremely rare. (medscape.com)
  • Antibodies are glycoproteins belonging to the immunoglobulin superfamily . (wikipedia.org)
  • Rat IgG1 is a subclass of immunoglobulin G (IgG) antibodies that are commonly used in research and diagnostics. (thermofisher.com)
  • Immunoglobulins (Igs), the term is sometimes used interchangeably with "antibodies," are glycoprotein molecules produced by B lymphocytes and plasma cells in response to an immunogen or after recognition of specific epitopes on the antigen. (medscape.com)
  • Antibodies belong to immunoglobulin family of protein and have 5 isotypes (G, A, M, E, D) with further subclasses of G and A. The classes are differentiated with the characteristics of the Ig heavy chain of the antibody. (medscape.com)
  • The following product was used in this experiment: Rat IgG1 kappa Isotype Control (eBRG1), PerCP-eFluor™ 710, eBioscience™ from Thermo Fisher Scientific, catalog # 46-4301-80, RRID AB_1944422. (thermofisher.com)
  • Applications Reported: PerCP-eFluor® 710 Rat IgG1 Isotype Control has been reported for use in flow cytometric analysis. (thermofisher.com)
  • Applications Tested: PerCP-eFluor® 710 Rat IgG1 Isotype Control has been tested by flow cytometric analysis of mouse splenocytes and normal human peripheral blood cells. (thermofisher.com)
  • The following product was used in this experiment: Rat IgG2a kappa Isotype Control (eBR2a), APC, eBioscience™ from Thermo Fisher Scientific, catalog # 17-4321-81, RRID AB_470181. (thermofisher.com)
  • Staining of C57BL/6 splenocytes with Anti-Mouse CD4 eFluor® 450 (Product # 48-5870) and 0.125 µg of Rat IgG2a K Isotype Control APC (Product # 17-4321-81) (left) or 0.125 µg of Anti-Mouse CD366 APC (right). (thermofisher.com)
  • Description: The monoclonal rat IgG2a kappa is useful as an isotype control immunoglobulin. (thermofisher.com)
  • Rat IgG2a is an immunoglobulin subclass that is commonly used in research and diagnostic applications. (thermofisher.com)
  • C57BL/6 splenocytes were stained with CD45R/B220 FITC and IgD (clone 11-26c.2a) PE/Cyanine7 (top) or rat IgG2a, κ PE/Cyanine7 isotype control (bottom). (biolegend.com)
  • C57BL/6 splenocytes were stained with CD45R/B220 APC and IgD (clone 11-26c.2a) Brilliant Violet 650™ (top) or rat IgG2a, κ Brilliant Violet 650™ isotype control (bottom). (biolegend.com)
  • In most cases, use of a sub-class of isotype immunoglobulin (e.g. mouse IgG2a or IgG2b) is not required. (vectorlabs.com)
  • To determine exercise induced changes in the adaptive immune system by measuring the immunoglobulin isotype and subclass response to an ultra-marathon. (bmj.com)
  • For other B-cell defects, such as specific Ig deficiencies (eg, immunoglobulin A [IgA] or immunoglobulin G [IgG] subclass deficiencies), refer to the article B-Cell Disorders . (medscape.com)
  • This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems monoclonal reagents. (rndsystems.com)
  • Monoclonal gammopathy of undetermined significance (MGUS) is a premalignant hematological condition arising from B‑cells, characterized by the presence of monoclonal immunoglobulin production, also known as paraprotein. (springermedizin.at)
  • Changes in surface immunoglobulin isotypes on purified anti- gen-bindi" by J J. Kenny, S W. Kessler et al. (jax.org)
  • Changes in surface immunoglobulin isotypes on purified anti- gen-binding cells after antigenic stimulation. (jax.org)
  • Early stages of B-cell differentiation can be identified by the status of the immunoglobulin genes and by the cell surface markers CD34, CD19, and surface immunoglobulin (sIg). (medscape.com)
  • Immunoglobulin D (IgD) is an antibody isotype typically expressed in the plasma membranes of naïve B cells, usually co-expressed with IgM. (rndsystems.com)
  • Be the first to review Mouse IgG2B Alexa Fluor® 405-conjugated Isotype Control and earn rewards! (rndsystems.com)
  • Have you used Mouse IgG2B Alexa Fluor® 405-conjugated Isotype Control? (rndsystems.com)
  • No. 555347) and either rat anti-human IL-4 (Alexa Fluor® 647-MP4-25D2) (left panel) or immunoglobulin isotype control (Alexa Fluor® 647-R3-34, Cat. (bdbiosciences.com)
  • Individuals with such an unusual profile should receive only low IgA intravenous immunoglobulin preparations. (medscape.com)
  • Therapies for these disorders (eg, intravenous immunoglobulin [IVIG], bone marrow transplantation, gene therapy) are very costly and require highly advanced facilities. (medscape.com)
  • During class switching, the constant region of the immunoglobulin heavy chain changes but the variable regions do not, and therefore antigenic specificity, remains the same. (wikipedia.org)
  • MZB1 is a GRP94 cochaperone that enables proper immunoglobulin heavy chain biosynthesis upon ER stress. (southernbiotech.com)
  • While class switch recombination is mostly a deletional process, rearranging a chromosome in "cis", it can also occur (in 10 to 20% of cases, depending upon the Ig class) as an inter-chromosomal translocation mixing immunoglobulin heavy chain genes from both alleles. (wikipedia.org)
  • B lymphocytes, named after their site of origin in the bursa of Fabricius in birds or in the bone marrow in humans, form the basis for humoral immunity by their production of immunoglobulins. (medscape.com)
  • Selective immunoglobulin A deficiency (SIgAD) is a primary immunodeficiency disease and is the most common of the primary antibody deficiencies. (medscape.com)
  • Most cases are agammaglobulinemia with autosomal recessive/dominant heritage and represent a very heterogeneous group, including immunoglobulin (Ig) deficiency with increased immunoglobulin M (hyper-IgM syndrome), which is also discussed separately (see X-linked Immunodeficiency With Hyper IgM ). (medscape.com)
  • In experienced ultra-endurance runners, alterations in immunoglobulin concentrations after a race suggest an enhanced immune response, including isotype switching, interactions with the innate immune system, and a secondary antibody response. (bmj.com)
  • Immunoglobulin (Ig) is the key component of humoral immunity. (medscape.com)
  • This control addresses whether tissue elements are inadvertently binding immunoglobulin from the same species as the primary antibody, in addition to non-specific binding from the secondary detection reagents. (vectorlabs.com)
  • The terms antibody and immunoglobulin are often used interchangeably, [1] though the term 'antibody' is sometimes reserved for the secreted, soluble form, i.e. excluding B-cell receptors. (wikipedia.org)
  • Memory B cells feature many discrete properties indicative of differentiation through ISOTYPE SWITCHING and/or IMMUNOGLOBULIN SOMATIC HYPERMUTATION for rapid and robust recall of specific antibody responses. (bvsalud.org)
  • Selected for use as mouse IgG 2B isotype control for flow cytometry applications. (rndsystems.com)
  • Isotype control should be used at the same concentration as experimental antibody. (thermofisher.com)
  • 2) Use of an isotype control (e.g. "non-immune" mouse IgG), matched to that of the primary antibody and applied at the same protein concentration as the primary antibody, is probably the most widely used negative control. (vectorlabs.com)
  • As with negative control no. 1 above, lack of staining indicates tissue elements are not binding this isotype of immunoglobulin. (vectorlabs.com)
  • therefore, cellular and molecular analyses of RTX and CA125 interaction were peformed, and a library of RTX variants was generated using a proprietary technology called Block‑Removed Immunoglobulin Technology that combines randomized amino acid substitutions and high‑throughput functional screenings to identify CA125‑refractory RTX variants. (spandidos-publications.com)
  • An antibody ( Ab ), also known as an immunoglobulin ( Ig ), [1] is a large, Y-shaped protein used by the immune system to identify and neutralize foreign objects such as pathogenic bacteria and viruses . (wikipedia.org)
  • Note that the use of pre-immune immunoglobulin, obtained prior to immunization, could also be used. (vectorlabs.com)
  • However, it is very unusual for commercial vendors to offer pre-immune immunoglobulin. (vectorlabs.com)
  • B-cell disorders are divided into defects of B-cell development/immunoglobulin production ( immunodeficiencies ) and excessive/uncontrolled proliferation ( lymphomas , leukemias ). (medscape.com)
  • Naïve mature B cells produce both IgM and IgD, which are the first two heavy chain segments in the immunoglobulin locus. (wikipedia.org)
  • Memory cells travel to the primary follicle, where, after exposure to dendritic cells, they differentiate into centroblasts (immunoglobulin class-switch). (medscape.com)
  • Common variable immunodeficiency (acquired or adult-onset hypogammaglobulinemia) is characterized by low immunoglobulin (Ig) levels with phenotypically normal B cells that can proliferate but do not develop into Ig-producing cells. (msdmanuals.com)
  • Abnormal PC immunophenotype (≥95% of BMPCs are clonal) and reduction of ≥1 uninvolved immunoglobulin isotype. (dana-farber.org)
  • The 11-26c.2a antibody reacts with immunoglobulin D in all tested mouse haplotypes. (biolegend.com)
  • The term "irrelevant" refers to a primary antibody of the same isotype as the specific primary antibody (i.e. mouse IgG) and applied at the same concentration, that is known not to bind to a target in the tissue specimen. (vectorlabs.com)
  • The quadrant markers for the bivariate dot plots were set based on the autofluorescence and isotype controls. (bdbiosciences.com)
  • During CSR, portions of the antibody heavy chain locus are removed from the chromosome, and the gene segments surrounding the deleted portion are rejoined to retain a functional antibody gene that produces antibody of a different isotype. (wikipedia.org)
  • In addition to the highly repetitive structure of the target S regions, the process of class switching needs S regions to be first transcribed and spliced out of the immunoglobulin heavy chain transcripts (where they lie within introns). (wikipedia.org)
  • Immunoglobulin gene rearrangement begins with heavy-chain gene rearrangement followed by light-chain gene rearrangement. (medscape.com)
  • Parasitologic and clinical human response to immunoglobulin administration in falciparum malaria. (ajtmh.org)
  • This graph shows the total number of publications written about "Immunoglobulin G" by people in this website by year, and whether "Immunoglobulin G" was a major or minor topic of these publications. (umassmed.edu)