First described in 1942, FITC is the original fluorescein molecule functionalized with an isothiocyanate reactive group (−N=C=S), replacing a hydrogen atom on the bottom ring of the structure. (wikipedia.org)
It is typically available as a mixture of isomers, fluorescein 5-isothiocyanate (5-FITC) and fluorescein 6-isothiocyanate (6-FITC). (wikipedia.org)
When confocal laser scanning images of tissues stained with fluorescein isothiocyanate (FITC)-conjugated UEA1 or WBA were compared with the appearance of the same tissues under the scanning electron microscope (SEM), UEA1 strongly stained 97.2% (106/109) of M-cells, 0.6% (3/516) enterocytes, and 0% (0/28) goblet cells, whereas WBA stained 100% (83/83) M-cells, 1.7% (6/361) enterocytes, and 5.3% (1/19) goblet cells. (nih.gov)
FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. (novusbio.com)
D) Representative flow cytometry plots of cells stained with fluorescein isothiocyanate (FITC)-conjugated anti-CD71 and phycoerythrin (PE)-conjugated anti-CD235a antibodies, demonstrating sequential expression of CD71 followed by CD235a and subsequent loss of CD71. (haematologica.org)
Permeability was assessed by the efflux of fluorescein isothiocyanate (FITC)-dextran permeated through the monolayer endothelial cells (ECs). (springer.com)
Fluorescein isothiocyanate conjugated (FITC) dextrans and ficolls of varying molecular weights (MWs), and FITC-bovine serum albumin (BSA) were employed using FRAP bleach areas of different diameters. (kcl.ac.uk)
The antibody reacts with both free and conjugated fluorescein isothiocyanate (FITC). (anobase.org)
Moreover, the separation of fluorescein isothiocyanate (FITC) and glutamic acid labeled with FITC was used to demonstrate fluorescence detection. (cdc.gov)
The most sensitive (99%) and specific (100%) method is reported to be the DFA test, which identifies oocysts in concentrated or unconcentrated fecal samples by using a fluorescein isothiocyanate (FITC)-labeled monoclonal antibody. (cdc.gov)
Using fluorescein isothiocyanate (FITC)-Ficoll 70 and albumin in phosphate buffered saline (PBS) as well as in bovine blood plasma, it is shown that CNT membranes can allow for significantly higher hydraulic permeability (more than an order of magnitude when normalized to pore area) than commercial high-flux hemofiltration/hemodialysis membranes (HF 400), as well as greatly enhance removal of middle molecules while maintaining comparable albumin retention. (lu.se)
BD Tritest™ CD4 fluorescein isothiocyanate (FITC)/CD8 phycoerythrin (PE)/CD3 peridinin chlorophyll protein (PerCP) is a three-color directimmunofluorescence reagent. (who.int)
Tetramethylrhodamine isothiocyanate1
The antibody does not react with bound or free TRITC (tetramethylrhodamine isothiocyanate). (anobase.org)
A succinimidyl-ester functional group attached to the fluorescein core, creating "NHS-fluorescein", forms another common amine reactive derivative that has much greater specificity toward primary amines in the presence of other nucleophiles. (wikipedia.org)
Dextran2
Dextran labeled with fluorescein isothiocyanate for possible use in perfusion studies in animals. (sigmaaldrich.com)
C-BF reduced LPS induced intestinal histological damage and gut permeability to 4 KD Fluorescein-isothiocyanate-conjugated dextran. (unboundmedicine.com)
Goat1
After incubation at 37 o C for 30 minutes, the slides are washed with PBS and normal yolk sac and fluorescein isothiocyanate-conjugated goat anti-human IgG (gamma-chain specific) added at the optimal dilution. (cdc.gov)
Fluorescence1
Due to the problem of photobleaching, derivatives of fluorescein such as Alexa 488 and DyLight 488 have been tailored for various chemical and biological applications where greater photostability, higher fluorescence intensity, or different attachment groups are needed. (wikipedia.org)
Antibody7
Two different conjugates - anti-bovine neutrophil elastase antibody-fluorescein isothiocyanate conjugate and anti-bovine neutrophil elastase antibody-Quantum Dots 710nm conjugate were prepared. (autocellcount.com)
Secondary antibody: Fluorescein goat secondary antibody at 1:1,000 for 60 min at RT. (genetex.com)
The serum globulin fractions were labeled either with fluorescein isothiocyanate or tetramethylrhodamine isothiocyanate, each of which produced brilliant fluorescent antibody staining of homologous erythrocytes. (ajtmh.org)
By pairing and mixing fluorescein labeled antibody to erythrocytes of one species with rhodamine labeled antibody to erythrocytes of another species, it was possible to test for two species with a single microscope slide preparation, thus allowing one to screen for 16 animal species with a blood meal extracted from a single mosquito. (ajtmh.org)
Language": "en", "Country": "XG", "Code": "Storage Conditions (Product)" }, { "Name": "Intended Use", "Value": "This antibody is intended for in vitro diagnostic (IVD) use. (roche.com)
The antibody is obtained through purification of the goat gamma globulin fraction, followed by reaction with fluorescein isothiocyanate. (roche.com)
Antibodies2
Language": "en", "Country": "XG", "Code": "Content" }, { "Name": "Background Information", "Value": "Fluorescent antibodies have been used to detect specific antigens in cells or tissue for over 40 years. (roche.com)
3. Preparation of fluorescein-isothiocyanate-labeled antibodies. (roche.com)
Derivatives1
Due to the problem of photobleaching, derivatives of fluorescein such as Alexa 488 and DyLight 488 have been tailored for various chemical and biological applications where greater photostability, higher fluorescence intensity, or different attachment groups are needed. (wikipedia.org)
Vivo1
We then evaluated intestinal permeability both in vivo and ex vivo using plasma fluorescein isothiocyanate-dextran and by assessing the paracellular permeability and transepithelial electrical resistance (TEER) in an Ussing chamber, respectively. (wellnessresources.com)
Determination1
Determination of trace deoxyribonucleic acid by using fluorescein isothiocyanate-phenosafranine as a double-luminescent phosphorescence probe. (bvsalud.org)
Fluoresce1
Fluorescein conjugates absorb light maximally at 492 nm and fluoresce maximally at 520 nm. (jacksonimmuno.com)
Group1
A succinimidyl-ester functional group attached to the fluorescein core, creating "NHS-fluorescein", forms another common amine reactive derivative that has much greater specificity toward primary amines in the presence of other nucleophiles. (wikipedia.org)