We sought to determine if intradermal injection of plasmid DNA encoding FGF-2 (pFGF) followed by noninvasive cutaneous electroporation (pFGFE+) could increase blood flow and angiogenesis in a rat model of hindlimbischemia. (odu.edu)
Our results suggest that pFGFE+ is a potential noninvasive, nonviral therapeutic approach to increase perfusion and angiogenesis for the treatment of limb ischemia. (odu.edu)
This study aimed to evaluate the effects of the E-twenty six (ETS) factor Ets variant 2 (ETV2) gene expression in angiogenesis and the effect of ETV2 gene therapy in a mouse model of hindlimbischemia. (vinastemcelllab.com)
In vivo knockdown of ETV2 expression inhibited the auto-recovery of ischemic hindlimb, while overexpression of ETV2 helped to rescue leg loss and reduce necrosis, significantly improving angiogenesis in hindlimbischemia. (vinastemcelllab.com)
Delivery of ADSC by subcutaneous transplantation of CS is effective for stimulation of angiogenesis and tissue protection in limb ischemia with a potential for efficacy improvement by BV transduction to express VEGF165. (biomedcentral.com)
Exogenous application of EDIL3 has been demonstrated to augment angiogenesis and improve blood flow and tissue function in murine models of hind-limb ischemia, cardiac ischemia and cerebral ischemia. (rndsystems.com)
CONCLUSIONS 476310-60-8 The outcomes of this research display that endogenous HMGB1 is vital for ischemia-induced angiogenesis in diabetic mice which HMGB1 proteins administration enhances security blood circulation in the ischemic hind limbs of diabetic mice through a VEGF-dependent system. (exposed-skin-care.net)
Hind7
We found that wild type (WT) C57BL/6 mice, subjected to hind limb ischemia (HLI) and supplemented with carnosine (1g/L) in drinking water, had improved blood flow recovery and limb function, enhanced revascularization and regeneration of myocytes compared with HLI mice placed on water alone. (frontiersin.org)
In vivo detection of macrophage recruitment in hind-limb ischemia using a targeted near-infrared fluorophore. (coolled.com)
Results: Hind limb ischemia was similar in ApoE-/- and C57 mice before the onset of exercise. (johnshopkins.edu)
A patch 3D printed with endothelial cells in a specific architecture helped reduce ischemia when used in mouse models of heart attack and hind limb ischemia, according to research published in Nature . (medpagetoday.com)
A diabetes-induced decrease in security vessel formation continues to be exhibited in murine versions: hind limb ischemia produced by femoral arteryligation is usually from the decreased development of capillaries and a decrease in blood flow towards the ischemic hind limb in diabetic versus non-diabetic mice (3). (exposed-skin-care.net)
Launch The breakthrough of endothelial progenitor cells, broadly thought as proangiogenic cells (PACs), described a new path in the treatment of cardiovascular disorders (CVDs), since they were recognized as responsible for postnatal vasculogenesis in physiological and regenerative neovascularization after, for example, hind limb ischemia (HLI) (5, 6). (mingsheng88.org)
Moreover, by showing that lack of Nrf2 did not impair revascularization after hind limb ischemia, despite decreased mobilization and angiogenic potential of proangiogenic cells (26)] has created great anticipations of PAC software. (mingsheng88.org)
Conclusions: Demand limb ischemia in the ApoE-/- phenotype exacerbated the expression of select systemic cytokines in plasma and blunted indices of muscle regeneration. (johnshopkins.edu)
In this work we report on therapeutic efficacy of CS from mouse ADSC transduced to express human vascular endothelial growth factor 165 a/a isoform (VEGF165), which showed potency to restore perfusion and protect tissue in a model of limb ischemia. (biomedcentral.com)
CS were transplanted subcutaneously to mice with surgically induced limb ischemia and followed by laser Doppler perfusion measurements. (biomedcentral.com)
Background: Chronic lower limb ischemia develops earlier and more frequently in patients with type 2 diabetes mellitus. (unav.edu)
The aim of this study is to evaluate the feasibility, safety, and efficacy of cell therapy based on mesenchymal stromal cells derived from adipose tissue intramuscular administration to patients with type 2 diabetes mellitus with critical limb ischemia and without possibility of revascularization. (unav.edu)
Discussion: Chronic lower limb ischemia has limited therapeutic options and constitutes a public health problem in both developed and underdeveloped countries. (unav.edu)
In this study, we use a well-established, severe femoral-saphenous artery-vein pair resection model of unilateral hindlimbischemia to test the relevance of Cx37 and Cx40 to postischemic tissue survival and recovery of limb perfusion. (figshare.com)
These results demonstrate that Cx40 is necessary for postischemic limb survival and reperfusion, whereas Cx37 deletion reduces the extent of ischemia in the same model. (figshare.com)
Endothelial2
The surgical procedure for delivery of embryonic stem cell-derived endothelial cells to the ischemic hindlimb is demonstrated, with non-invasive tracking by bioluminescence imaging. (jove.com)
Flow cytometry demonstrated that the number of EPC-like cells (Sca-1/Flk-1) in peripheral blood increased in wild-type mice after hindlimbischemia surgery and exogenous vascular endothelial growth factor stimulation, but not in MMP-9 mice. (tmu.edu.tw)
Increased poly-ADP-ribose activity and mature muscle regeneration were associated with demand ischemia in the C57BL6 mice, compared with the ApoE-/- mice (P = 0.01). (johnshopkins.edu)
Neural JNK3 regulates blood flow recovery after hindlimbischemia in mice via an Egr1/Creb1 axis. (harvard.edu)
Short-term cardiac stress, induced by ischemia-reperfusion (I/R) injury resulted in impaired left ventricular (LV) recovery and increased infarct size in heterozygous Hmox1-deficient (Hmox1 +/− ) mice [ 55 ]. (springer.com)
METHODS AND RESULTS-: Hindlimbischemia surgery was conducted in MMP-9 mice and wild-type (MMP-9) mice. (tmu.edu.tw)
Plasma levels and bone marrow concentrations of soluble Kit-ligand (sKitL) were significantly elevated in wild-type mice in response to tissue ischemia, but not in MMP-9 mice. (tmu.edu.tw)
Murine1
In a severe murine hindlimbischemia model, HGF delivery from the affinity-binding system improved tissue blood perfusion and induced mature blood vessel network formation. (tau.ac.il)
Surgically induced1
We report the use of muscle-derived MSCs to treat surgically-induced hindlimbischemia in a mouse model of type 1 diabetes (DM-1). (thno.org)
CONCLUSIONS-: These findings suggest that MMP-9 deficiency impairs ischemia-induced neovascularization, and these effects may occur through a reduction in releasing the stem cell-active cytokine, and EPC mobilization, migration, and vasculogenesis functions. (tmu.edu.tw)
Cerebral6
However, the combined effect of prophylactic zinc administration and therapeutic taurine treatment on intrauterine ischemia- (IUI-) induced cerebral damage remains unknown. (hindawi.com)
These results collectively indicate the chronicity of oxidative stress and an inadequate antioxidant response after a cerebral hypoxia-ischemia event and have motivated the development of preventive and therapeutic approaches against oxidative stress. (hindawi.com)
Preterm Fetal Hypoxia-Ischemia Causes Hypertonia and Motor Deficits in the Neonatal Rabbit: A Model for Human Cerebral Palsy? (jneurosci.org)
Prenatal hypoxia-ischemia to the developing brain has been strongly implicated in the subsequent development of the hypertonic motor deficits of cerebral palsy (CP) in premature and full-term infants who present with neonatal encephalopathy. (jneurosci.org)
Hypoxia-ischemia (H-I) is also associated with subsequent cerebral injury in a disproportionately high percentage of the survivors of premature birth ( Volpe, 2001 ). (jneurosci.org)
Most previous studies have employed models of cerebral hypoxia-ischemia in postnatal animals. (jneurosci.org)
Skeletal muscle1
Background: We designed studies to determine whether the ApoE-/- phenotype modulates the local skeletal muscle and systemic inflammatory (plasma) responses to lower extremity demand ischemia. (johnshopkins.edu)
Angiogenic1
In this study, we tested the hypothesis that carnosine protects against post ischemia by augmenting HIF-1α angiogenic signaling by Fe 2+ chelation. (frontiersin.org)
It is crucial for PACs to survive in conditions of the increased production of reactive oxygen species (ROS) that accompany ischemia and/or inflammatory response and be able to participate in tissue repair. (mingsheng88.org)
Extremities1
As a result, the blood flow to patients' extremities can become progressively impaired, and ischemia can occur. (thno.org)
Model3
These findings provide a unique behavioral model to define mechanisms and sequelae of perinatal brain injury from antenatal hypoxia-ischemia. (jneurosci.org)
The essential role of vascular maturation was demonstrated in the rabbit hindlimbischemia model. (dpz.eu)
Moreover, its importance in blood vessel formation, vascular restoration, and functioning of PACs was indicated in different animal models, such as wire-induced carotid artery injury model, HLI, or retinal ischemia (13, 33C35, 47). (mingsheng88.org)
Reactive1
In neonatal hypoxia-ischemia, an increase in reactive oxygen species (ROS) is known to occur in the brain at 96 h after a hypoxic-ischemic episode, to which the antioxidant system responds by increasing the activity of superoxide dismutase (SOD) and catalase (CAT) at postnatal day (PND) 11, which decreases afterward [ 5 ]. (hindawi.com)
Tissues3
Ischemia is the reduction of blood flow to tissues by injury of blood vessels. (vinastemcelllab.com)
however, its insufficiency increases muscle blood circulation under tissues ischemia. (mingsheng88.org)
Hypoxic1
Hypoxic-ischemic encephalopathy (HIE) following intrauterine ischemia (IUI) is one of the leading causes of brain injury in neonates, and the effects can endure until adulthood [ 1 , 2 ]. (hindawi.com)
Phenotype1
Uterine ischemia in the dams resulted in a distinct neurobehavioral phenotype in the newborn pups, which was characterized by an increase in forelimb tone that was significantly correlated with histological evidence of persistent injury to subcortical motor pathways involving the basal ganglia and anterior thalamus. (jneurosci.org)
Efficacy1
The ETV2 viral vector was injected into the skeletal muscles at the ligated and burned sites of the hindlimb and evaluated for its efficacy as a gene therapy modality for ischemia. (vinastemcelllab.com)
Rabbit1
In the present study, a new neurobehavioral battery of tests was used to characterize the motor performance of newborn pups after sustained hypoxia-ischemia to the preterm rabbit fetus at 67-70% gestation. (jneurosci.org)
Severe1
The major indications for replantation in the absence of prolonged ischemia, segmental damage, and diffuse crush or severe avulsion injuries are amputations of the thumb, multiple digits, a digit in a child, and a digit distal to the flexor digitorum superficialis insertion. (medscape.com)