StrainsPathogenTranscriptionalProteinsBiosynthesisViralRNAsPathogensBiosyntheticExpressionRegulatoryBacteriaAntibiotic resistanceBiofilmMicrobiologyRegulationPhenotypesPathwaysMechanismsClusterRiboflavinPeptideMembraneClustersEncodesDiversitySequencesCambridgeMolecularStudyCellSmallTargetPotentialLargeStructureTransferProcessesIdentifyComplexTransport
Strains4
- Roseoflavin biosynthesis genes have been expressed in riboflavin- or FMN-overproducing yeast strains of Candida famata and Komagataella phaffii . (biomedcentral.com)
- For comparison, recombinant strains of the native bacterial producer S. davaonensis accumulated near one-order less of roseoflavin while no recombinant producers of aminoriboflavin was reported at all. (biomedcentral.com)
- Interestingly, s479 is not only encoded next to CRISPR-cas genes but the mature s479 contains a crRNA-like 5´ handle and experiments with Cas protein deletion strains indicate maturation by Cas6 and interaction with Cas proteins. (uni-stuttgart.de)
- Rhizospheric region is regarded as the host or theatre of plant-microbe interactions and as a machinery for the recovery of new bacterial genes and strains. (springeropen.com)
Pathogen5
- Bacterial leaf blight (BLB) pathogen, Xanthomonas oryzae pv. (bvsalud.org)
- oryzae (Xoo) is the most devastating bacterial pathogen, which jeopardizes the sustainable rice (Oryza sativa L.) production system. (bvsalud.org)
- Moreover, MeEIL5 promoted the physical interaction of MeHsf20 and pathogen-related gene 3 (MePR3), resulting in improved anti-bacterial activity of MePR3. (bvsalud.org)
- such as a bacterial pathogen or parasite adapting to a host) as well as the discovery of novel factors associated with pathogenesis (toxins and pathogenesis/virulence regulation systems). (ukm.my)
- When genes encoding virulence factors are inactivated, virulence in the pathogen is diminished. (pressbooks.pub)
Transcriptional4
- MeEIL5 physically interacted with MeHsf20 to promote the transcriptional activation of the gene encoding N-acetylserotonin O-methyltransferase 2 (MeASMT2), thereby improving melatonin accumulation. (bvsalud.org)
- MicroRNAs regulate gene expression by inhibiting the post-transcriptional process: miRNA pairs with messenger RNA (mRNA) to its untranslated regions, which leads to formation of miRNA-induced silencing complex (miRISC) that includes ribonucleases. (turkupetcentre.net)
- Therefore, this complex regulatory network requires precise control of gene expression patterns at both transcriptional and post-transcriptional levels in order to ensure proper maturation, differentiation, and formation of blood and lymphatic vessels. (nature.com)
- These genes are regulated by a transcriptional factor known as peptide chain release factor 1 (PrfA). (pressbooks.pub)
Proteins4
- Three of the six up-regulated genes encode potential zinc transporter proteins (ZnuA1, ZnuB1, ZnuC1) suggesting involvement of s479 in regulation of zinc transport. (uni-stuttgart.de)
- Allostery - This is a type of effect seen in proteins where the binding of a molecule, ion etc. to one location can have an impact of the structure and interactions at another location. (rcsb.org)
- Structural Mechanisms of Cooperative DNA Binding by Bacterial Single-Stranded DNA-Binding Proteins. (jhmi.edu)
- Genetic studies of osmoregulation in yeast have identified mucin-like proteins as critical regulators of osmosensitive signaling and gene expression. (prolekarniky.cz)
Biosynthesis1
- This study illustrates the dual roles of MeEIL5 in fine-tuning MeHsf20-mediated coordination of melatonin biosynthesis and anti-bacterial activity, highlighting the ethylene-responsive MeEIL5 as the integrator of ethylene and melatonin signals in the immune response in cassava. (bvsalud.org)
Viral2
- Antisense imaging may therefore be most successful in detecting viral or bacterial mRNAs that are abundant in infected tissue. (turkupetcentre.net)
- b) In transduction, a bacteriophage injects DNA that is a hybrid of viral DNA and DNA from a previously infected bacterial cell. (pressbooks.pub)
RNAs1
- Recent studies have revealed the importance of long noncoding RNAs (lncRNAs) as tissue-specific regulators of gene expression. (nature.com)
Pathogens1
- The top highly abundant rhizospheric soil non-redundant ARGs that are prevalent in bacterial human pathogens or colonizers (commensal) included mtrA , soxR, vanRO , golS , rbpA , kdpE , rpoB2 , arr-1 , efrA and ileS genes. (springeropen.com)
Biosynthetic4
- The effects of RoF and its immediate biosynthetic precursor AF on the bacterial growth were studied. (biomedcentral.com)
- Hans-Wilhelm Nützmann from the University of Bath leads the next study that looks at the co-regulation of clustered biosynthetic pathway genes. (garnetcommunity.org.uk)
- Nützmann HW, Doerr D, Ramírez-Colmenero A, et al (2020) Active and repressed biosynthetic gene clusters have spatially distinct chromosome states Proc Natl Acad Sci U S A. 2020;201920474. (garnetcommunity.org.uk)
- They use Hi-C and related techniques to study the control of expression of clustered biosynthetic pathway genes in Arabidopsis. (garnetcommunity.org.uk)
Expression6
- RT-PCR showed that compound A20 inhibited the expression of T3SS-related genes. (bvsalud.org)
- This comprehensive global TSS mapping atlas provides a valuable resource for RNA biology and gene expression analysis in the Enterococci. (frontiersin.org)
- Deep RNA-DNA, RNA-protein interaction studies, and phenotype rescue analyses reveal that LETR1 is a nuclear trans-acting lncRNA modulating, via key epigenetic factors, the expression of essential target genes, including KLF4 and SEMA3C , governing the growth and migratory ability of LECs. (nature.com)
- In particular, the FANTOM (Functional Annotation of the Mammalian Genome) consortium pioneered the discovery of the noncoding RNA world by providing, through Cap Analysis of Gene Expression (CAGE-Seq), the first evidence that large portions of our genome are transcribed, producing a multitude of sense and antisense transcripts 12 . (nature.com)
- In osm-8 mutants, normal physiological responses to hypertonic stress, such as the accumulation of organic osmolytes and activation of osmoresponsive gene expression, are constitutively activated. (prolekarniky.cz)
- After screening ∼18,000 gene knockdowns, we identified 27 suppressors that specifically affect the constitutive osmosensitive gene expression and Osr phenotypes of osm-8 mutants. (prolekarniky.cz)
Regulatory2
- antimicrobial resistance (AMR) families of the resistance mechanism of antibiotic efflux pump included resistance-nodulation-cell division (RND) antibiotic efflux pump (for mtrA , soxR and golS genes), major facilitator superfamily (MFS) antibiotic efflux pump (for soxR gene), the two-component regulatory kdpDE system (for kdpE gene) and ATP-binding cassette (ABC) antibiotic efflux pump (for efrA gene). (springeropen.com)
- Determinants of target prioritization and regulatory hierarchy for the bacterial small RNA SgrS. (jhmi.edu)
Bacteria3
- Analyzing bacteria in late exponential phase, we capture ~40% ( E. faecalis ) and 43% ( E. faecium ) of the annotated protein-coding genes, determine 5′ and 3′ UTR (untranslated region) length, and detect instances of leaderless mRNAs. (frontiersin.org)
- targets can be anything from small ions such as Zn 2+ to viruses and bacteria. (turkupetcentre.net)
- Yeasts have many advantages as biotechnological producers relative to bacteria, however, no recombinant producers of bacterial antibiotics in yeasts are known. (biomedcentral.com)
Antibiotic resistance2
- A better understanding of how these species cause disease and spread antibiotic resistance requires a knowledge of how its genes are controlled, on both the DNA and the RNA level. (frontiersin.org)
- A metagenomic whole genome shotgun sequencing approach was used for rhizospheric soil micribiome of the wild plant Abutilon fruticosum in order to detect antibiotic resistance genes (ARGs) along with their antibiotic resistance mechanisms and to detect potential risk of these ARGs to human health upon transfer to clinical isolates. (springeropen.com)
Biofilm1
- Several studies have illustrated the therapeutic efficacy of photoactivated riboflavin against nosocomial infections and multidrug resistant bacterial infections as well as microbial associated biofilm infections, revealing the potential role of riboflavin as a promising antimicrobial candidate, which could serve as one of the alternatives in fighting the global crisis of the emergence of antimicrobial resistance seen in different pathogenic microbes. (bioseek.eu)
Microbiology1
- BAC-TO-FUEL is a multidisciplinary project which brings together leaders in the fields of materials chemistry, computational chemistry, chemical engineering, microbiology and bacterial engineering. (bactofuel.eu)
Regulation2
- Transcriptome studies of wild type Haloferax cells and the deletion mutant revealed up-regulation of six genes in the deletion strain, showing that the sRNA has a clearly defined function. (uni-stuttgart.de)
- For instance, the nucleus requires controlled conditions for precise regulation of the genes inside. (jove.com)
Phenotypes2
- To identify genes required for Osm-8 phenotypes, we performed a genome-wide RNAi osm-8 suppressor screen. (prolekarniky.cz)
- HGT allows even distantly related species to share genes, influencing their phenotypes. (pressbooks.pub)
Pathways2
- 2017 ). Furthermore, mWGS can accurately assign microbial function and contribution to the intact environment and technically allows the study of soil resistome, CAZymes (Carbohydrate Active enZymes) and KEGG (Kyoto Encyclopedia of Genes and Genomes) pathways. (springeropen.com)
- To counteract decreases in cell volume caused by hypertonic conditions, cells restore volume through the rapid accumulation of ions and water via the activation of various plasma membrane ion conductance pathways [2] . (prolekarniky.cz)
Mechanisms2
- This study reveals potential mechanisms that suggest gene clustering in the one-dimensional chromosome is accompanied by compartmentalization of the 3D chromosome. (garnetcommunity.org.uk)
- There are three prokaryote-specific mechanisms leading to horizontal gene transfer in prokaryotes. (pressbooks.pub)
Cluster3
- The sRNA gene is encoded between a CRISPR RNA locus and the Cas protein gene cluster, the s479 deletion strain is viable and was characterized in detail. (uni-stuttgart.de)
- AMR families of the resistance mechanism of antibiotic target alteration included glycopeptide resistance gene cluster (for vanRO gene), rifamycin-resistant beta-subunit of RNA polymerase (for rpoB2 gene) and antibiotic-resistant isoleucyl-tRNA synthetase (for ileS gene). (springeropen.com)
- A cluster of virulence genes encoded on a pathogenicity island is responsible for the pathogenicity of L. monocytogenes . (pressbooks.pub)
Riboflavin1
- Genes FMN1, rosB, rosC and rosA encoding riboflavin kinase (EC 2.7.1.26), 8-demethyl-8-amino-riboflavin-5′-phosphate synthase (EC 2.6.1.114), AF-phosphate phosphatase and dimethyltransferase (EC 2.1.1.343), respectively, and also are responsible for synthesis of roseoflavin. (biomedcentral.com)
Peptide1
Membrane1
- The final two papers are focused on research in wheat, first led from the University of Leicester that looks at recombination in durum wheat and secondly from Rothamsted in which they have identified a whole family of NPF membrane transporter genes. (garnetcommunity.org.uk)
Clusters1
- To resolve this issue a new electrochemical cell was designed and constructed, which helps to increase the concentration of clusters and to decrease the unreduced ions. (bactofuel.eu)
Encodes1
- One of the genes regulated by PrfA is hyl , which encodes a toxin known as listeriolysin O (LLO), which allows the bacterium to escape vacuoles upon entry into a host cell. (pressbooks.pub)
Diversity1
- In prokaryotes, horizontal gene transfer (HGT), the introduction of genetic material from one organism to another organism within the same generation, is an important way to introduce genetic diversity. (pressbooks.pub)
Sequences1
- alignment - A comparison of two or more gene or protein sequences in order to determine their degree of similarity in amino acid or bases, respectively. (rcsb.org)
Cambridge1
- The final 'methods-type' paper is from Alison Smith's group in Cambridge and has developed a riboswitch-based resource for use in the model alga Chlamydomonas reinhardtii . (garnetcommunity.org.uk)
Molecular1
- A pathogen's virulence factors are encoded by genes that can be identified using molecular Koch's postulates. (pressbooks.pub)
Study1
- During the last 15 years, SPR has been intensively used in the study of bacterial secretion systems due to its ability of detecting highly dynamic complexes, which are difficult to investigate by other techniques. (bvsalud.org)
Cell1
- For axenic growth under laboratory conditions, it specifically relies on the external supply of N -acetylmuramic acid (MurNAc), which is an essential constituent of the peptidoglycan (PGN) of bacterial cell walls. (karger.com)
Small1
- Subsequent movements even happen within small chemical microcosms called vesicles while en route to their final destinations. (jove.com)
Target1
- AMR families of the resistance mechanism of antibiotic target protection included bacterial RNA polymerase-binding protein (for RbpA gene), while those of the resistance mechanism of antibiotic inactivation included rifampin ADP-ribosyltransferase (for arr-1 gene). (springeropen.com)
Potential1
- In summary, this work exemplifies the potential of the title compound as an inhibitor of T3SS and its efficacy in the control of bacterial leaf blight. (bvsalud.org)
Large2
- Further increasing the surface area failed due to the large amount of unreduced ions in the solutions which negatively influence the photocatalytic activities. (bactofuel.eu)
- The type I and II introns and bacterial Ribonuclease P are large ribozymes. (jove.com)
Structure3
- The ApcT structure reveals an inward-facing, apo state and an amine moiety of Lys158 located in a position equivalent to the Na2 ion of LeuT. (tcdb.org)
- Crystal structure and ligand-induced folding of the SAM/SAH riboswitch. (jhmi.edu)
- Increased ion accumulation raises cytoplasmic ionic strength, which can disrupt protein structure and function [3] . (prolekarniky.cz)
Transfer3
- Typically, when we consider genetic transfer, we think of vertical gene transfer, the transmission of genetic information from generation to generation. (pressbooks.pub)
- Vertical gene transfer is by far the main mode of transmission of genetic information in all cells. (pressbooks.pub)
- When prokaryotes and eukaryotes reproduce asexually, they transfer a nearly identical copy of their genetic material to their offspring through vertical gene transfer. (pressbooks.pub)
Processes1
- Here, we show that mutations in the Caenorhabditis elegans mucin-like gene osm-8 specifically disrupt osmoregulatory physiological processes. (prolekarniky.cz)
Identify1
- They also identify the related ALP2 gene and find that it interacts with MULTICOPY SUPPRESSOR OF IRA1 (MSI1) as part of the PRC2. (garnetcommunity.org.uk)
Complex1
- Everyone is aware that even the simplest bacterial genomes are complex and beyond our full understanding. (fieldofscience.com)
Transport1
- They proposed that Lys158 is central to proton-coupled transport and that the amine group serves the same functional role as the Na2 ion in LeuT, thus demonstrating common principles among proton- and sodium-coupled transporters. (tcdb.org)