• reagents
  • Immunoassays may be run in multiple steps with reagents being added and washed away or separated at different points in the assay. (wikipedia.org)
  • Some immunoassays can be carried out simply by mixing the reagents and sample and making a physical measurement. (wikipedia.org)
  • These enzymes allow for detection often because they produce an observable color change in the presence of certain reagents. (wikipedia.org)
  • In some cases these enzymes are exposed to reagents which cause them to produce light or Chemiluminescence. (wikipedia.org)
  • If the assay involves addition of exogenous reactants (the reagents), then their quantities are kept fixed (or in excess) so that the quantity (and quality) of the target is the only limiting factor for the reaction/assay process, and the difference in the assay outcome is used to deduce the unknown quality or quantity of the target in question. (wikipedia.org)
  • employ
  • Enzymes adopt a specific three-dimensional structure, and may employ organic (e.g. biotin) and inorganic (e.g. magnesium ion) cofactors to assist in catalysis. (primidi.com)
  • occur
  • Since enzymes are selective for their substrates and speed up only a few reactions from among many possibilities, the set of enzymes made in a cell determines which metabolic pathways occur in that cell. (primidi.com)
  • work
  • Yalow accepted the Nobel Prize for her work in immunoassays in 1977, becoming the second American woman to have won the award. (wikipedia.org)
  • Like all catalysts, enzymes work by lowering the activation energy ( E a ‡) for a reaction , thus dramatically increasing the rate of the reaction. (primidi.com)
  • type
  • The most common problem due to hepatitis C but not involving the liver is mixed cryoglobulinemia (usually the type II form) - an inflammation of small and medium-sized blood vessels. (wikipedia.org)
  • chemical
  • Thus reading of an assay may be quite noisy and may involve greater difficulties in interpretation than an accurate chemical titration. (wikipedia.org)
  • cell
  • BACKGROUND/AIMS The Fas-Fas ligand system is thought to be involved in stromal cell apoptosis after corneal wounding. (bmj.com)
  • thought
  • By 2012, the commercial immunoassay industry earned US$17,000,000,000 and was thought to have prospects of slow annual growth in the 2 to 3 percent range. (wikipedia.org)