• PCR analysis of exon utilization reveals massive alternative mRNA splicing of Sec31B, with just 16 exons being constitutively utilized in all transcripts. (biologists.com)
  • The presence of a stop codon in exon 13 generates two families of Sec31B gene products (each displaying additional patterns of mRNA splicing): a group of full-length proteins (hereafter referred to as Sec31B-F) and also a group of truncated proteins (hereafter referred to as Sec31B-T), distinguished by their utilization of exon 13. (biologists.com)
  • ETS-1 and ETS-2 mRNA and protein levels were much higher in the ocular tissues of Tyrp-1-TAg mice than in control ocular tissues from wild-type mice. (molvis.org)
  • Since the introduction of RNA sequencing (RNA-seq) as a high-throughput mRNA expression analysis tool, this procedure has been increasingly implemented to identify cell-level transcriptome changes in a myriad of model systems. (molcells.org)
  • random to their competing genomic online artificial risk and the attP of the such trial in suppressor target pIRES2-EGFP-TSLC1 trait4 residues are again associated for the conditionsPrivacy of sequences for collection with a mRNA of elsewhere 50 polynucleotide among the polynomial differences( Figure 1). (scoutconnection.com)
  • Limited microarray analysis of mRNA expression in freshly isolated CM and CF showed that while certain genes are highly expressed in CM many growth factors cytokines and ECM genes are more highly expressed in CF16. (abt-888.net)
  • In the present study we analyzed AS and gene expression transitions regulated during postnatal mouse heart development using mRNA deep sequencing (RNA-seq)22. (abt-888.net)
  • The kinetic parameters of recombinant proteins showed that ZmHXK4-7 have high catalytic efficiency for Glc, fructose (Fru) and mannose (Man), ZmHXK7 has a lower Km for ATP, and together with ZmHXK8 they have lower sensitivity to inhibition by ADP, G6P and N-acetylglucosamine than ZmHXK4-6 and ZmHXK9. (biomedcentral.com)
  • In this study, a suppression subtractive hybridization cDNA library approach was used for the identification of pathogen and plant genes that were differentially expressed during infection of the susceptible cultivar BRS Pérola of Phaseolus vulgaris L. A total of 979 unigenes (430 contigs and 549 singletons) were obtained and classified according to their functional categories. (frontiersin.org)
  • It provides a very informative, albeit preliminary, glimpse into the expression of genes encoding functionally relevant proteins as potential targets for chemotherapy, and evidence for the presence of a relict plastid. (biomedcentral.com)
  • For triterpene saponin biosynthesis, we found the genes in MVA and non-MVA pathways were differentially expressed among three examined tissues, indicating the parallel but compartmentally separated biosynthesis pathways of IPP and DMAPP in A. mongolicus . (biomedcentral.com)
  • With 35,000 genes and hundreds of thousands of protein states to identify, correlate, and understand, it no longer suffices to rely on studies of one gene, gene product, or process at a time. (genomicglossaries.com)
  • Using this system for the in vivo analysis of the spatial and temporal patterns of cell cycle dynamics, we demonstrated that Inhibitors,Modulators,Libraries Tax expressing cells arrest in the G1 phase of the cell cycle and proceeded to apop tosis, and we found that Tax induced changes in the expression of genes related to cell cycle regulation and apoptosis correlated well with the morphological changes observed in the cells. (thrombininhibitors.com)
  • We analyzed a comprehensive GC cell line database to identify the specifically expressed genes in gastric SRCC. (sdlifesciences.com)
  • Previously we showed that genes that undergo AS regulation during heart development produce transitions from GSK1324726A embryonic to adult protein isoforms GSK1324726A largely without changes in overall transcript levels presenting a new paradigm for understanding developmentally regulated gene expression in heart3. (abt-888.net)
  • To reduce the risk of analyzing cell line- pathway can be divided into distinct stages based on the specific features, we used several representative cell lines for recombination status of the Ig genes and on the expression each of four major stages in B cell development: pro-B, pre-B, pattern of surface markers and the presence of intracellular proteins [1- 6]. (lu.se)
  • Chlamydia trachomatis is an obligate intracellular human pathogen that exhibits stage-specific gene transcription throughout a biphasic developmental cycle. (plos.org)
  • Most NLRs guard host proteins that are the direct targets of pathogen effectors. (ubc.ca)
  • Rapid and comprehensive analysis of putative proteinous allergens ( allergenome ) by applying such a proteomic strategy … With allergenomics, we can not only detect and assign the putative allergens (proteins specifically interacting with IgE antibodies in a patient's blood) in a short time, but also analyze the quantitative and qualitative change of the antigens, depending on the surroundings and environmental conditions of an allergenic causative. (genomicglossaries.com)
  • the putative Sm USP transcripts analysed were detected in all investigated stages, showing distinct expression during S. mansoni development. (biomedcentral.com)
  • In vitro analysis revealed that a putative switch-protein kinase regulator, RsbW, is capable of interacting directly with σ 66 , as well as phosphorylating its own antagonist, RsbV1, rendering it inactive. (plos.org)
  • RNA sequencing revealed that the pathways involved in intracellular calcium regulation were significantly upregulated in MUC20v2-overexpressing GC cells. (sdlifesciences.com)
  • Limb-girdle muscular dystrophy (LGMD) protein defects occur in several pathways involved in the biologic function of muscle and can be divided into groups based on cellular localization. (medscape.com)
  • We used LOV-Turbo for pulse-chase labeling to discover proteins that traffic between endoplasmic reticulum, nuclear and mitochondrial compartments under cellular stress. (bvsalud.org)
  • Furthermore, GSOs protected cells against GLU-induced apoptosis by reducing the expression of the mitochondrial apoptosis-associated Bcl-2 family effector proteins and protected cells from GLU-induced oxidative damage by increasing the nuclear translocation of Nrf2 and HO-1 expression. (sdbonline.org)
  • The Drosophila retinoblastoma protein, Rbf1, induces a debcl and drp1 -dependent mitochondrial apoptosis. (sdbonline.org)
  • These results provide a mechanism by which Drosophila Bcl-2 family proteins can control apoptosis and shed light on a link between Rbf1 and mitochondrial dynamics, in vivo. (sdbonline.org)
  • Mitochondrial porin , also known as the voltage-dependent anion channel (VDAC), is a multi-functional channel protein that shuttles metabolites between the mitochondria and the cytosol and implicated in cellular life and death decisions. (sdbonline.org)
  • A collection of different allotypes or allelic protein variants, a new type of protein library. (genomicglossaries.com)
  • Homer protein homolog 1 or Homer1 is a neuronal protein that in humans is encoded by the HOMER1 gene. (wikipedia.org)
  • As reported, miR-25 and miR-92a show clear nuclear enrichment in rat primary cortical neurons by using a combination of microarray analysis and small RNA deep sequencing [ 10 ]. (biomedcentral.com)
  • We used RNA sequencing technology to characterize blood progenitor cells and MCs in nasal polyps. (harvard.edu)
  • The cytokine encoded by this gene induces changes in intracellular calcium concentration and enzyme release in monocytes. (creativebiomart.net)
  • Here, we report a molecular technology for stable genetic tagging of cells that exhibit activity-related increases in intracellular calcium concentration (FLiCRE). (bvsalud.org)
  • When expressed as a fusion protein with eYFP in cultured cells, Sec31B-F associates with the endoplasmic reticulum and with vesicular-tubular clusters, displays restricted intracellular movement characteristic of COPII vesicle dynamics, co-distributes on organelles with Sec13, Sec31A and Sec23 (markers of the COPII coat), and concentrates with ts045-VSV-G-CFP (VSV-G) when examined early in the secretory pathway or after temperature or nocodazole inhibition. (biologists.com)
  • Results Tax induces cell cycle arrest and apoptosis in transfected HeLa cells To examine whether Tax induces cell cycle Inhibitors,Modulators,Libraries arrest at the G1 phase and promotes apoptosis in HeLa cells, chimeric Tax carrying a Flag tag at the carboxyl terminus was transfected into HeLa cells. (thrombininhibitors.com)
  • The incorporation of light-responsive domains into engineered proteins has enabled control of protein localization, interactions and function with light. (bvsalud.org)
  • especially, in localization, Nonetheless small lipid requires purified digested to the protein of the most TSLC1 random expression to be the two figures. (scoutconnection.com)
  • Plant intracellular nucleotide binding and leucine-rich repeat proteins (NB-LRR, NLRs) function as immune receptors to detect microbial pathogens directly or indirectly. (ubc.ca)
  • The current studies provide novel insights into the pharmacology of GPR40 and indicate that G protein-coupled receptors which respond to fatty acids, and potentially to other lipid ligands, can be occupied by endogenous agonists before assay and that this may mask the pharmacology of the receptor and may be mistaken for high levels of constitutive activity. (aspetjournals.org)
  • In chapter 2, by using CRISPR/Cas9 gene editing, genetic analysis and biochemical assays, I identified the differential pairings of typical NLR receptor SOC3 with atypical NLR proteins CHS1 or TN2 to guard the homeostasis of the E3 ligase SAUL1. (ubc.ca)
  • different online artificial receptor, longitudinal property and 0K1hisds+∫ backbone of sequence restriction of areas,4,368 data: pFlpBtM-II for expression Steroid, 2000 Mol. (scoutconnection.com)
  • The protein encoded by this gene is a cytokine receptor that belongs to the interleukin 1 receptor family. (creativebiomart.net)
  • This protein binds interleukin alpha (IL1A), interleukin beta (IL1B), and interleukin 1 receptor, type I(IL1R1/IL1RA), and acts as a decoy receptor that inhibits the activity of its ligands. (creativebiomart.net)
  • What are the functional implications of IL1R2's intracellular domain, and how can engineered receptor constructs shed light on its contributions? (creativebiomart.net)
  • Some sequences are similar to those proven to be targets for effective intervention in other protozoan parasites, and include not only proteases, antioxidant enzymes, and heat shock proteins, but also those associated with relict plastids, such as acetyl-CoA carboxylase and methyl erythrithol phosphate pathway components, and those involved in glycan assembly, protein folding/secretion, and parasite-host interactions. (biomedcentral.com)
  • The covalent modification of proteins by the addition or removal of ubiquitin, a highly conserved protein comprising of 76 amino acids, changes the molecular function of the target protein and can therefore influence its interactions with other proteins. (biomedcentral.com)
  • Interestingly, functionally redundant SNIPER1 and SNIPER2 can control the protein levels of diverse sNLRs and the interactions between SNIPER1 and sNLRs appear to be through the common nucleotide-binding (NB) domains of sNLRs. (ubc.ca)
  • Nowadays, my group develops researches in genomic and transcriptome annotation and mainly in structural bioinformatics, with development of algorithms for analyses of protein-protein interactions, and protein-DNA interactions. (cnpem.br)
  • Many of the protein-protein interactions (PPI) or intra protein interactions are mediated by interactions between domains and specific patterns of sequence. (cnpem.br)
  • Using semi-quantitative reverse-transcription polymerase chain reaction (RT-PCR) and western blots experiments, we compared changes in ETS-1 and ETS-2 expression, their protein levels, and the regulation of some of their target gene expressions at different stages of the ocular tumoral progression in the transgenic mouse model, Tyrp-1-TAg, with those in normal eyes from control mice of the same age. (molvis.org)
  • S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. (cancerindex.org)
  • Under ABA treatment, the NR proteins are degraded via a 26S-proteasome dependent manner, while the transcriptional regulation is the main manner to rapidly reduce the NIA1 levels under nitrogen deficiency and NaCl stress conditions. (sdlifesciences.com)
  • Protein kinases have an important role in cell signaling by coordinating various cellular processes, including metabolism, growth, division, differentiation, mobility, transport through membranes, contraction, and apoptosis being phosphorylation the most abundant and important type of cellular regulation. (cnpem.br)
  • These results identify potential roles for AS in multiple aspects of postnatal heart maturation including vesicular trafficking and intracellular membrane dynamics. (abt-888.net)
  • These AS changes are enriched as targets of the CUGBP ELAV-Like family GSK1324726A (Celf) and Muscleblind-like (Mbnl) RNA-binding protein families both of which are involved in AS and are regulated during postnatal heart development3 23 In the heart vesicular trafficking-related AS transitions likely impact ligand/growth factor uptake ion channels GSK1324726A dynamics and/or postnatal. (abt-888.net)
  • The RNA-binding protein NANOS3 poses as an interesting candidate gene for POI since members of the Nanos family have an evolutionarily conserved function in germ cell development and maintenance by repressing apoptosis. (hindawi.com)
  • The identification of an inactivating missense mutation in NANOS3 suggests a mechanism for POI involving increased primordial germ cells (PGCs) apoptosis during embryonic cell migration and highlights the importance of NANOS proteins in human ovarian biology. (hindawi.com)
  • Exhibiting high consistence in sequence and structure, S100 family members are interchangeable in function and they show a wide spectrum of biological processes, including proliferation, apoptosis, migration, inflammation and differentiation and the like. (cancerindex.org)
  • Both proteins can localize at the mitochondrion , but the way they control apoptosis still remains unclear. (sdbonline.org)
  • In mice, Nanos3 is expressed in the primordial germ cells (PGCs) from their formation until shortly after their appearance in the gonads (E13.5 in female and E14.5 in male embryos) [ 24 ]. (hindawi.com)
  • In cells lacking expression of Ca 2+ -mobilizing G proteins, coexpression of human GPR40 and Gα q allowed medium- and long-chain fatty acids to elevate intracellular [Ca 2+ ]. (aspetjournals.org)
  • This was also observed when human embryonic kidney (HEK) 293 cells were transfected with a GPR40-Gα q fusion protein. (aspetjournals.org)
  • Membranes of rat INS-1E cells that express GPR40 endogenously provided similar observations. (aspetjournals.org)
  • by direct sequencing and investigated the role of MUC20 in GC progression using GC cells with MUC20 silencing and forced expression. (sdlifesciences.com)
  • Moreover, we found that gastric SRCC cells specifically expressed MUC20v2, which was dominantly expressed in the cytoplasm. (sdlifesciences.com)
  • One of the most recent advancements in this field includes in situ combinatorial indexing, which fixes cells to confine the RNA within them, then uses a split-and-pool technique (the cells are sequentially divided into well plates then recombined several times) to attach barcodes and adapter sequences in several combinations. (molcells.org)
  • In this study, the miRNAs and their target mRNAs in the Argonaute2 (Ago2) complex of nucleus and cytoplasm of gastric cancer cells were characterized using high-throughput sequencing of RNAs isolated by crosslinking immunoprecipitation (HITS-CLIP). (biomedcentral.com)
  • Further characterization showed that susa2-2 only suppresses the autoimmunity mediated by either CHS1-SOC3 or TN2-SOC3 paired NLR proteins, indicating that SUSA2 is specifically involved in NLR protein SOC3-mediated immunity. (ubc.ca)
  • The identification and characterization of geometrical and physical-chemical properties in protein vacant spaces aggregates important information for steering rational drug design and functional characterization of binding and catalytic sites. (cnpem.br)
  • The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. (cancerindex.org)
  • High sequence homology is mainly observed in two regions that surround the catalytic Cys and His residues: the so-called Cys Box domains, containing 19 amino acids, and the His Box domains, containing 60-90 amino acids [ 8 - 10 ]. (biomedcentral.com)
  • This is the first study describing the production and distribution of ETS-1 and ETS-2 mRNAs and proteins using in situ hybridization and immunohistochemistry in murine ocular tissue sections of normal control eyes and tumoral eyes from mice of the same age. (molvis.org)
  • The protozoan parasite Perkinsus marinus is a facultative intracellular parasite of mollusks, and the causative agent of "dermo" disease in both wild and farmed Eastern oyster ( Crassostrea virginica ) populations along the Atlantic and Gulf coasts of the USA [ 1 - 4 ]. (biomedcentral.com)
  • Unlike such genetic changes, epigenetic changes, which do not alter genome sequence, can result in three possible outcomes: passive dilution, active repair through negative feedback, or active maintenance through positive feedback. (nature.com)
  • However, in the mid 1990s, other proteins were also found to accumulate in the abnormal muscle fibers, and molecular genetic studies revealed several chromosomal loci. (medscape.com)
  • The double-stranded RNA duplex leads RNA-induced silencing complex (RISC), containing a member of the conserved Argonaute (Ago) protein family, to target sites of mRNAs, resulting in the destabilization of the mRNAs and/or inhibition of translation [ 4 , 5 ]. (biomedcentral.com)
  • Also, through crosslinking another multimeric protein Shank, it is proposed to comprise a core of the postsynaptic density. (wikipedia.org)
  • present the first comprehensive attempt to use a synthetic protein to bridge the pre- and postsynaptic membranes1. (bvsalud.org)
  • encodes a protein that is related to a large family of proteins that consist of a proline-rich or glycine-rich N-terminus and a hydrophobic, possibly membrane spanning C-terminus. (or.jp)
  • The kinetic of elevation of intracellular [Ca 2+ ] slowed with increasing fatty acid chain length, suggesting different ligand on-rates, whereas the addition of fatty acid-free bovine serum albumin reduced signals, presumably by binding the fatty acids. (aspetjournals.org)
  • At the sub-cellular scale, maps document the spatial organization of proteins, RNA, DNA, and metabolites with nanometer precision and temporal acuity on the order of seconds. (stanford.edu)
  • and membrane organization These AS transitions are enriched among targets of two RNA-binding proteins Celf1 and Mbnl1 which undergo developmentally regulated changes in expression. (abt-888.net)
  • Biotinylated Recombinant human CCL14 protein was tag free expressed. (creativebiomart.net)
  • We have studied the transgenic mouse strain, Tyrp-1-TAg, to try to gain insight into possible molecular mechanisms common to pigmented ocular neoplasms occurring spontaneously in the eyes of these mice and human choroidal melanoma. (molvis.org)
  • Northern analysis detected a specific 2.5-kilobase pair hOAT1 transcript only in human kidney. (aspetjournals.org)
  • Immunoblot analysis of human kidney cortex demonstrated that hOAT1 is an 80- to 90-kilodalton heterogeneous protein modified by abundant N -glycosylation. (aspetjournals.org)
  • Recombinant human IL1R2 protein with His tag was expressed in HEK293. (creativebiomart.net)
  • Read-through transcripts are also expressed that include exons from the upstream cytokine gene, chemokine (C-C motif) ligand 15, and are represented as GeneID: 348249. (creativebiomart.net)
  • This study provides evidence that a switch-protein kinase regulatory network controls availability of σ 66 , the main sigma subunit for transcription in Chlamydia . (plos.org)
  • Gene therapy displacement of endogenous MCUb with a dominant-negative MCUb transgene (MCUb W246R/V251E ) in vivo rescued T2D cardiomyocytes from metabolic inflexibility and stimulated cardiac contractile function and adrenergic responsiveness by enhancing phospholamban phosphorylation via protein kinase A. We conclude that MCUb represents one newly discovered molecular effector at the interface of metabolism and cardiac function, and its repression improves the outcome of the chronically stressed diabetic heart. (diabetesjournals.org)
  • Members of the evolutionarily conserved Nanos gene family are preferentially expressed in the ovaries and are known to play an important role in germ cell development, maintenance, and survival [ 24 - 30 ]. (hindawi.com)
  • Based on evolutionarily conserved seed sequence and free binding energy, computational predictions have greatly improved the potentials to find bona fide miRNA target sites [ 15 ]. (biomedcentral.com)
  • We found circulating MCps expressing MC-associated markers. (harvard.edu)
  • It has been previously shown that rbf1 pro-apoptotic activity depends on its ability to decrease the level of anti-apoptotic proteins such as the Bcl-2 family protein Buffy. (sdbonline.org)
  • Buffy often acts opposite to Debcl , the other Drosophila Bcl-2-family protein. (sdbonline.org)
  • Cross-talk between phosphorylation and ubiquitination is a significant aspect of intracellular signalling networks [ 12 , 13 ]. (biomedcentral.com)
  • SUSA2 encodes an F-box protein Actin-Related Protein 8 (ARP8) and SUSA3 encodes the chaperone protein HSP90.3. (ubc.ca)
  • Our observations suggest that the HXKs may be redundant proteins with specific roles depending on carbon and ATP availability, metabolic needs, or sensor requirements. (biomedcentral.com)
  • Homer1 protein has an N-terminal EVH1 domain, involved in protein interaction, and a C-terminal coiled-coil domain involved in self association. (wikipedia.org)
  • Homer1a, acts as a natural dominant negative form that blocks interaction between long-forms and their ligand proteins by competing with the EVH1 binding site on the ligand proteins. (wikipedia.org)
  • The substituted amino acid is located within the second C2HC motif in the conserved zinc finger domain of NANOS3 and in silico molecular modelling suggests destabilization of protein-RNA interaction. (hindawi.com)
  • Distribuition of domain interaction energy in a multidomain proteins. (cnpem.br)
  • In wild Saccharomyces , where changes in protein folding can persist for many generations, the folded structures of prion proteins template the folding of newly made proteins 5 . (nature.com)
  • These protein structures recurring in proteomes are known as conserved domains. (cnpem.br)
  • Serum expressed against structural Pso online artificial photosynthesis 2016 1 time with young Der connection 1 and increased Pso 5log1+t+bi1t+bi0+ε 1 to transfer estimated to the replacement of P. Moredun Research Institute, Pentlands Science Park, Bush Loan, Penicuik, EH26 0PZ, Scotland. (scoutconnection.com)
  • then, introns distributed to joint online artificial photosynthesis 2016 integrase like the Helmholtz Protein Sample Production Facility( PSPF) have a longitudinal site of blunt mortality Carriers obtaining changes, structural construct survival and the embodiment efficiency RRS exchange( BEVS). (scoutconnection.com)
  • Rare mutations in genome sequence that result from failed DNA repair are transmitted across generations through DNA replication during each cell division. (nature.com)
  • Scope includes mutations and abnormal protein expression. (cancerindex.org)
  • The EST analysis reported herein, together with the recently completed sequence of the P. marinus genome and the development of transfection methodology, should result in improved intervention strategies against dermo disease. (biomedcentral.com)
  • Using whole-genome sequencing of OS, we found features of TP53 intron 1 rearrangements suggesting a unique mechanism correlated with transcription. (oncotarget.com)
  • Click Gene ID to show a list of GSM assays in which the gene are specifically expressed. (or.jp)
  • We also demonstrated upregulation of ETS-1 and ETS-2 target expressions in Tyrp-1-TAg mice when comparing with the same target expressions in control mice. (molvis.org)
  • A model is proposed where the relative levels of active antagonist (RsbV1) and switch-protein anti-sigma factor (RsbW) control the availability of σ 66 and subsequently act as a molecular 'throttle' for Chlamydia growth and development. (plos.org)
  • These include proteins associated with the sarcolemma (see image below), proteins associated with the contractile apparatus (see image below), and various enzymes involved in muscle function. (medscape.com)
  • This clone was engineered to express the complete ORF with an expression tag. (origene.com)
  • Through this approach, we identified MCps characterized by expression of MC-associated transcripts and a discrete surface and intracellular protein expression profile. (harvard.edu)
  • These MCps shared transcriptional and protein expression characteristics with proliferating MCs within the nasal polyps, suggesting a link between the two. (harvard.edu)