Interacts histone acetyltransferase activity protein binding. Medical search. Frequent questions

The fact that histones are modified by acetylation has been known for almost 30 years. (embl-heidelberg.de)
The recent identification of enzymes that regulate histone acetylation has revealed a broader use of this modification than was suspected previously. (embl-heidelberg.de)
Acetylation regulates many diverse functions, including DNA recognition, protein-protein interaction and protein stability. (embl-heidelberg.de)
Histone acetylation is important in chromatin remodelling and gene activation. (embl-heidelberg.de)
Collectively, these results suggest that targeted histone acetylation by recruited HAT cofactors and histone deacetylation are important factors affecting PR transactivation. (drugbank.com)
Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. (cancerindex.org)
In htz1Delta cells, Sir2 and Sir3 spread into flanking euchromatic regions, producing changes in histone H4 acetylation and H3 4-methylation indicative of ectopic heterochromatin formation. (sdbonline.org)
Post-translational modifications (PTMs) of proteins, including phosphorylation, acetylation, ubiquitination, and SUMOylation, can regulate the function of proteins, determine the active state and subcellular location of proteins, and dynamically interact with other proteins related to carcinogenesis and progression ( 17 - 20 ). (frontiersin.org)
Inhibition of core histones acetylation by carcinogenic nickel(II). (nih.gov)
2002. Acetylation of interferon regulatory aspect-7 by p300/CREB-binding proteins (CBP)-associated aspect (PCAF) impairs its DNA Tasquinimod binding. (isme-la2019.org)
2007. Legislation of P-TEFb elongation complicated activity by CDK9 acetylation. (isme-la2019.org)
Post-translational modification of proteins at lysine residues by reversible acetylation is catalyzed by the opposing activities of histone acetyltransferases (HATs) and histone deacetylases (HDACs), which act on both histone and non-histone substrates despite their names. (biomedcentral.com)
Chd1 chromodomain links histone H3 methylation with SAGA- and SLIK-dependent acetylation. (yeastgenome.org)
2022 ). Among all types of histone modifications, the reversible N-terminal lysine acetylation, regulated by histone acetyltransferase (HAT) and histone deacetylase (HDAC), is one of the best-characterized machineries (Kurdistani and Grunstein 2003 ). (biomedcentral.com)
Cell viability/proliferation assays, cell cycle analysis by flow cytometry, immunodetection of specific histone and p53 acetylation marks, western blotting, siRNA and RT-qPCR. (biomedcentral.com)
In addition to its effects on histone modifications, garcinol was found to block CBP/p300-mediated acetylation of the C-terminal activation domain of p53, but resulted in enhanced acetylation of p53K120, and accumulation of p53 in the cytoplasmic compartment. (biomedcentral.com)
SIRT1 substrates in the mammalian nucleus Potential SIRT1 substrates could be discovered by acquiring proteins that talk about the SIRT1 identification theme, bind overexpressed SIRT1 or display elevated degrees of acetylation in SIRT1-depleted cells. (monossabios.com)
SIRT1 interacts with BMAL1-CLOCK straight, getting rid of CLOCK-mediated acetylation of histone H3-Lys9 (H3K9), histone H3-Lys4 (H3K4), and BMAL1 Lys537. (monossabios.com)
and they are associated with altered histone acetylation and transcription factor (nerve growth factor-induced clone A [NGFIA]) binding to the glucocorticoid receptor promoter. (deepdyve.com)

One of the isoforms has been shown to preclude binding of one of the other TFIID subunits, thereby reducing transcription and initiating signals that trigger apoptosis. (wikipedia.org)

Transcription initiation factor TFIID subunit 6 is a protein that in humans is encoded by the TAF6 gene. (wikipedia.org)
This gene encodes one of the smaller subunits of TFIID that binds weakly to TBP but strongly to TAF1, the largest subunit of TFIID. (wikipedia.org)
The KAT6B gene provides instructions for making a type of enzyme called a histone acetyltransferase. (medlineplus.gov)
Little is known about the function of the histone acetyltransferase produced from the KAT6B gene. (medlineplus.gov)
Studies suggest that these mutations likely prevent the production of functional histone acetyltransferase from one copy of the KAT6B gene in each cell. (medlineplus.gov)
These changes likely alter histone modification, which could prevent normal regulation of gene activity. (medlineplus.gov)
Thus, the bromodomain is functionally linked to the HAT activity of co-activators in the regulation of gene transcription. (embl-heidelberg.de)
The human GR protein is produced from 9 exons of the GR gene and is composed of 3 major subdomains, each of which has specific structural and functional properties. (brainimmune.com)
Further, the GR gene expresses a splicing variant GRbeta, which does not bind glucocorticoids but functions as a dominant negative isoform for GRalpha. (brainimmune.com)
1,2 However, recent work has shown that chromatin, the RNA-DNA-protein complex that controls chromosomal organization and gene expression in mammalian nuclei, 3-5 blocks access of Cas9 to certain DNA target sites in vitro 6-8 and in mammalian cells. (aip.org)
The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family. (cancerindex.org)
What does this gene/protein do? (cancerindex.org)
What pathways are this gene/protein implicaed in? (cancerindex.org)
Here, we investigate how perturbation of the cofactor E2a affects global patterns of Smad2 /3 binding and gene expression during gastrulation. (xenbase.org)
E2a Is Required for Subsets of Smad2/3 Binding and for Global Patterns of Smad2/3 Target Gene Expression (A) Smad2/3 targets 1,027 distinct genomic regions in control stage 10.5 embryos (yellow) and 1,671 regions in E2a -depleted embryos (blue). (xenbase.org)
The variant histone H2A.Z regulates gene transcription, and deletion of the gene encoding H2A.Z strongly increases the requirement for SNF/SWI and SAGA. (sdbonline.org)
The conserved histone variant H2AZ has an important role in the regulation of gene expression and the establishment of a buffer to the spread of silent heterochromatin. (sdbonline.org)
Recent studies have revealed that variants of histone H2A and histone H3 play important roles not only in gene expression but also in the repair of DNA breaks and the assembly of chromosome centromeres. (sdbonline.org)
The activity of the p53 gene product is regulated by a plethora of posttranslational modifications. (rupress.org)
This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2B family. (nih.gov)
This gene is found in the small histone gene cluster on chromosome 6p22-p21.3. (nih.gov)
The human H2A and H2B histone gene complement. (nih.gov)
Histone H1-dependent chromatin dynamics have been shown to be important in a variety of biological phenomena and in the transcriptional regulation of a certain gene (20-24). (techblessing.com)
A transcriptional co-activator complex that accompanies Pol II during elongation, preferentially acetylates nucleosomal histones H3 and H2B and subsequently evicts nucleosomes from gene coding regions. (yeastgenome.org)
Histone-modifying enzymes, which regulate eukaryotic chromatin conformation and gene expression, are key epigenetic factors controlling fungal development, virulence, and secondary metabolism. (biomedcentral.com)
To dissect the epigenetic regulation of GLS pathogenesis, we characterized a histone deacetylase gene Cfhos2 in Colletotrichum fructicola , the causing agent of GLS. (biomedcentral.com)
Histone Mouse monoclonal to ERN1 deacetylation is certainly of particular significance in the modulation of gene appearance, as it could alter nucleosome framework and will often affect transcription [13] potentially. (monossabios.com)
The RI histone variants have an uneven distribution across the genome, carry specific posttranslational modifications (PTMs), and can affect gene expression by altering the chromatin state [ 3 ]. (biomedcentral.com)
Using semi-quantitative reverse-transcription polymerase chain reaction (RT-PCR) and western blots experiments, we compared changes in ETS-1 and ETS-2 expression, their protein levels, and the regulation of some of their target gene expressions at different stages of the ocular tumoral progression in the transgenic mouse model, Tyrp-1-TAg, with those in normal eyes from control mice of the same age. (molvis.org)
Chromatin immunoprecipitation assay was performed to evaluate the histone modification of gene loci that are regulated by lunasin and cytokine. (weeksmd.com)
This protein interaction variation is able to be monitored on a genomic scale via the change of gene pair co-expression. (biomedcentral.com)

8-11 A series of in vitro studies used reconstituted nucleosomes to demonstrate that Cas9/gRNA binding and cleaving are completely blocked at nucleosome-bound DNA. (aip.org)
Nucleosomes impose a block to transcription that can be overcome in vivo by remodeling complexes such as SNF/SWI and histone modification complexes such as SAGA. (sdbonline.org)
Histone variant H2A.Z (Htz1 in yeast) replaces conventional H2A in many nucleosomes. (sdbonline.org)
How histone variants such as H2AZ are incorporated into nucleosomes has been obscure. (sdbonline.org)
These advances establish a third mechanism of chromatin reconfiguration, raising fundamental questions about the stability of nucleosomes in nonreplicative phases of the cell cycle and the cellular machinery responsible for incorporating histone variants into nucleosomes (Mizuguchi, 2004). (sdbonline.org)
Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. (nih.gov)
The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. (nih.gov)

Molecular analysis suggested that Esc2p contributed to silencing in a manner similar to Sir1p and probably helped recruit or stabilize the other Sir proteins, while Htz1p present at HMR assembled a specialized chromatin structure necessary for silencing (Dhillon, 2000). (sdbonline.org)
These results describe a novel pathway for regulating transcription using variant histones to modulate chromatin structure (Santisteban, 2000). (sdbonline.org)
In addition TAF-I KD and histone H1 KD affected the chromatin structure near the promoters. (techblessing.com)
On the basis of these findings we propose that TAF-I and its target histone H1 are key regulators of the chromatin structure at the promoter to maintain the silent state of ISG transcription. (techblessing.com)
Linker histone H1 binds near to the entry and exit sites of the nucleosome core particle (NPC) which consists of a 147-base-pair (bp)-long deoxyribonucleic acid (DNA) wrapped around a histone octamer consisting of two copies each of the core histone proteins H2A H2B H3 and H4 and facilitates the higher order chromatin structure (17-19). (techblessing.com)
Epigenetic regulation refers to the multiple chemical modifications of DNA or DNA-associated proteins that alter chromatin structure and DNA accessibility in a heritable manner, without changing DNA sequence. (frontiersin.org)

SARS-CoV-2 helicase, the non-structural protein 13 (nsp13) is a highly conserved protein among all known coronaviruses, and, at the moment, is one of the most explored viral targets to identify new possible antiviral agents. (bvsalud.org)
The histone H2A.F/Z (H2AZ) variant is a functionally distinct, highly conserved histone subgroup that likely represents a separate evolutionary lineage of histone H2A proteins. (sdbonline.org)
For example, transcript elongation factor TFIIS is highly conserved among eukaryotes, and yet the TFIIS protein from Saccharomyces cerevisiae cannot function with mammalian RNA polymerase II and vice versa. (embl.de)
In human, AEG-1 represents a protein of 582 amino acids, and the amino acid sequences are highly conserved across vertebrates. (biomedcentral.com)
Functional proteomics and immunofluorescence analyses of H3.1 and H3.3 revealed a highly conserved role for Nrp1 and Asf1 histone chaperones in nuclear influx of histones. (biomedcentral.com)

These enzymes modify histones, which are structural proteins that attach (bind) to DNA and give chromosomes their shape. (medlineplus.gov)
To counteract constraints imposed by nucleosome structure, cells deploy two major classes of multiprotein enzymes, which covalently modify the nucleosome core histones or catalyze nucleosome mobility in an adenosine triphosphate (ATP)-dependent fashion. (sdbonline.org)
Garcinol treatment alters expression of chromatin modifying enzymes in MCF7 cells, resulting in reprogramming of key histone and p53 PTMs and growth arrest, underscoring its potential as a cancer chemopreventive agent. (biomedcentral.com)
Enzymes that modify chromatin and associated proteins by the addition or removal of acetyl or methyl groups play a key role in genome regulation [ 1 ]. (biomedcentral.com)
Thus, drug-like molecules that target chromatin modifying enzymes to reprogram selected histone PTMs in tumour cells may have potential as cancer chemopreventive agents. (biomedcentral.com)

BRG1 an adenosine triphosphate (ATP)-dependent nucleosome remodeling factor and a subunit of the SWI/SNF complex interacts with STAT2 in response to IFN facilitates the chromatin remodeling of the promoter region and promotes ISG transcription (13-15). (techblessing.com)
The RPB9 subunit of RNA polymerase II regulates transcription elongation activity and is required for the action of the transcription elongation factor, TFIIS. (embl.de)
We describe the cognate cDNA cloning and characterization of two subunits (hTFIIIC63 and hTFIIIC102) that are present within a DNA-binding subcomplex (TFIIIC2) of TFIIIC and are related in structure and function to two yeast TFIIIC subunits (yTFIIIC95 and yTFIIIC131) previously shown to interact, respectively, with the promoter (A box) and with a subunit of yeast TFIIIB. (embl.de)

STATs interact with histone acetyltransferases (HATs) including p300/CBP and GCN5 and GCN5 acetylates histones on the promoter in an IFN-dependent manner (6 7 Interestingly inhibition of histone deacetylase (HDAC) activity which opposes activity of HAT leads to a global impairment of the ISG transcription (8-10). (techblessing.com)

P-TEFb is also involved in cotranscriptional histone modification, mRNA processing and mRNA export (PubMed:15564463, PubMed:19575011, PubMed:19844166). (wuxibiortus.com)
SUMOylation is a post-translational modification of proteins that has been found to play a major role in the Wnt/β-catenin signaling pathway. (frontiersin.org)
These studies suggest that ISG transcription via promoters is under the control of the N-Methyl Metribuzin combined effects of histone modification and specific chromatin structures. (techblessing.com)
In this study we assessed the effects of the phytocompounds garcinol and curcumin on histone and p53 modification in cancer cells, focussing on the breast tumour cell line MCF7. (biomedcentral.com)
Ubiquitination is a multifunctional posttranslational modification controlling the activity, subcellular localization and stability of proteins. (cipsm.de)

For example, in humans, H3.1 and H3.2 are two RD histones, whereas H3.3 is an RI variant histone. (biomedcentral.com)

Much of the current understanding of these processes is derived from analyses of nucleosomal histones that represent the major histone species within cells. (sdbonline.org)

HDACs function by removing acetyl residues from the ε-amino group of lysine residues in the histone N-terminal tail, which restores the positive charge on the histone (Lai et al. (biomedcentral.com)

Interestingly, the analogous residues in TFIIS are also critical for elongation activity. (embl.de)

which have a variety of binding sites for sequence-specific transcription factors and specify characteristic chromatin structures mediated by nucleosome positioning specific histone modifications histone variants and other factors (1 2 For the transcription of type-I interferon (IFN)-stimulated genes (ISGs) promoters containing sequence motifs known as IFN-stimulated response element (ISRE) are the binding sites of the sequence-specific transcription factors activated by IFN stimulation. (techblessing.com)

Phosphorylation of eIF2α on Ser51 inhibits 5' cap-dependent mRNA translation, resulting in the global suppression of protein synthesis to facilitate adaptation to a variety of stresses linked to protein synthesis, including proteotoxic stress, viral replication, heme depletion and amino acid withdrawal [ 2 ]. (biomedcentral.com)
integrates phosphorylation during transcription with chromatin modifications to control co-transcriptional histone mRNA processing (PubMed:15564463, PubMed:19575011, PubMed:19844166). (wuxibiortus.com)
The phosphorylation of MYOD1 enhances its transcriptional activity and thus promotes muscle differentiation (PubMed:12037670). (wuxibiortus.com)
Catalyzes phosphorylation of KAT5, promoting KAT5 recruitment to chromatin and histone acetyltransferase activity (PubMed:29335245). (wuxibiortus.com)
SIRT1 substrates likewise incorporate the different parts of the AMP-activated proteins kinase (AMPK)-mediated cAMP and phosphorylation sensing cascade, including AMPK, forkhead container O transcriptional elements (FOXOs), the transcription coactivator PCG1alpha as well as the transcription aspect HIF1alpha [14,15]. (monossabios.com)

Structure and ligand of a histone acetyltransferase bromodomain. (embl-heidelberg.de)
Using an in vitro transcription assay, we found that progesterone receptor (PR)-driven transcription is inhibited by a dominant negative PR ligand-binding domain-interacting region of SRC-1A, indicating that SRC-1A is required for actual transcriptional processes. (drugbank.com)
Signaling is initiated when the Wnt ligand binds to the Frizzled receptor on the cell membrane and the LDL receptor-associated protein 5/6 (LRP5/6) co-receptor. (frontiersin.org)

This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. (cancerindex.org)
Swr1 is required for the deposition of histone H2AZ at specific chromosome locations in vivo, and Swr1 and H2AZ commonly regulate a subset of yeast genes. (sdbonline.org)
As the SEC can regulate the transcriptional activity of RNA polymerase II, it was hypothesized that these MLL-fusions induce aberrant regulation of transcriptional elongation on MLL-target genes 14 . (nature.com)
Histone H2B monoubiquitination functions cooperatively with FACT to regulate elongation by RNA polymerase II. (nih.gov)
In addition to the catalytic domain, eukaryotic sirtuins contain variable amino- and carboxy-terminal extensions that regulate their subcellular localizations and catalytic activity. (biomedcentral.com)
The primary actions that regulate circadian rhythms, although mediated by nonhomologous proteins, are conserved in progression [16] highly. (monossabios.com)

We also tested DNA-binding, transiently expressed activation-associated proteins (AAPs) that are known to support an open, transcriptionally active chromatin state. (aip.org)
Domain I also interacts with the transcriptionally active RNA polymerase II holoenzyme and therefore, may have a function unrelated to the previously described transcription elongation activity of TFIIS. (embl.de)
Mammalian H3.1 and H3.2 are deposited by the heterotrimeric chromatin assembly complex 1 (CAF1), whereas H3.3 is deposited at transcriptionally active regions by the Histone Regulator A (HIRA) complex [ 15 , 16 ]. (biomedcentral.com)

By adding a small molecule called an acetyl group to particular locations on histones, histone acetyltransferases control the activity of certain genes. (medlineplus.gov)
It is postulated to bind as a tetramer to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. (affbiotech.cn)
C) DAVID clustering analysis shows enrichment for developmental (dev) terms in all Smad2/3-associated genes and for genes that have stable Smad2/3 binding when E2a is depleted (red box in B) but not other subcategories of Smad2/3 binding. (xenbase.org)
D) Genes that retain Smad2/3 binding at the same genomic coordinates in E2a -depleted embryos (red box in B) are more likely to be downregulated by 2-fold or more in E2a -depleted embryos. (xenbase.org)
In addition to its role in H3K36 tri-methylation, SETD2 is required to maintain high H3K79 di-methylation and MLL-AF9-binding to critical target genes, such as Hoxa9 . (nature.com)
120 different translocations, resulting in the generation of MLL-fusion proteins encompassing more than 75 different partner genes 5 . (nature.com)
Consistent with previous long noncoding RNA studies, these lncRNAs overall have fewer exons, are shorter in length than protein-coding genes (pcGenes), and have relatively low expression level. (stanford.edu)
Eukaryotic expression vectors containing genes encoding plant proteins for killing of cancer cells. (weeksmd.com)
consequently, genes encoding some of these proteins are being used to design constructs for the inhibition of multiplying cancer cells. (weeksmd.com)

In addition, these coactivators also possess intrinsic histone acetyltransferase (HAT) activity and bind to each other and another HAT, p300/CBP-associated factor. (drugbank.com)
Here we show that the human PR also interacts with p300/CBP-associated factor in vitro. (drugbank.com)

We present the first comprehensive survey of protein-protein interactions of seven distantly related MLL-fusion proteins. (nature.com)
To date, most of our understanding of epigenetic regulation and its participation in leukemogenesis is based on the enzymatic activities and protein-protein interactions of histone and DNA modifiers. (frontiersin.org)
Using the yeast structure as a template, we have modeled the homologous domains from elongin A and CRSP70 and identified a conserved positively charged patch on the surface of all three proteins, which may be involved in conserved functional interactions with the transcriptional machinery. (embl.de)

Quantitative proteomic analysis of post-translational modifications of human histones. (nih.gov)
Post-translational modifications (PTMs) of histones and other proteins are perturbed in tumours. (biomedcentral.com)
Although treatment with curcumin, garcinol or the garcinol derivative LTK-14 hampered MCF7 cell proliferation, differential effects of these compounds on histone modifications were observed. (biomedcentral.com)
Histone modifications act as signals that are 'read' by sensory proteins containing bromodomains, PHD fingers and other domains, many of which function as coregulators of DNA-binding transcription factors [ 4 ]. (biomedcentral.com)
Such dynamic regulation is, in part, achieved through epigenetic modifications and selective incorporation of histone variants into chromatin. (biomedcentral.com)

It has histone deacetylase activity and represses transcription when tethered to a promoter. (cancerindex.org)
The E7 oncoprotein associates with Mi2 and histone deacetylase activity to promote cell growth. (virosin.org)

The experimental investigation of the binding mode revealed ATP-non-competitive kinetics of inhibition, not affected by substrate-displacement effect, suggesting an allosteric binding mode that was further supported by molecular modelling calculations predicting the binding into an allosteric conserved site located in the RecA2 domain. (bvsalud.org)

Many proteins involved in eukaryotic transcription are similar in function and in sequence between organisms. (embl.de)
Histones are conserved eukaryotic proteins that pack DNA into a compact structure called chromatin. (biomedcentral.com)

This information is based on mapping of SMART genomic protein database to KEGG orthologous groups. (embl-heidelberg.de)
Smad2/3 targets in which binding is at the same genomic position in control and E2a -depleted embryos are more likely to be downregulated in E2a -depleted embryos. (xenbase.org)
Families of related leukemia fusion proteins that share genomic and biological properties represent unique opportunities to study how the combination of distinct functional protein modules can drive oncogenic transformation. (nature.com)
2014 ). Different HDACs interact with different sets of proteins and form distinct HDAC protein complexes, which function differently in terms of the histone and lysine sites being modified, the targeted genomic regions, and the associated biological effects (Jeon et al. (biomedcentral.com)
Histone PTMs are also critical for other genomic functions, such as DNA replication and induction of repair mechanisms at sites of DNA damage [ 3 ]. (biomedcentral.com)

E2a changes the position of Smad2 /3 binding at the Nodal inhibitor lefty , resulting in direct repression of lefty that is critical for mesendoderm specification. (xenbase.org)
Mutations in the major core histones relieve transcriptional repression and bypass the requirement for SNF/SWI and SAGA. (sdbonline.org)
2022 ). Such positive charge generally facilitates histone-DNA binding, thereby favors chromatin condensation and transcriptional repression (Bannister and Kouzarides 2011 ), but in rare case leads to transcription activation as well (Wang et al. (biomedcentral.com)
These and other PTMs generate a combinatorial histone code that demarcates chromatin regions for transcription activation or repression [ 2 ]. (biomedcentral.com)

Silent information regulator 2 (Sir2) proteins, or sirtuins, are protein deacetylases dependent on nicotine adenine dinucleotide (NAD) and are found in organisms ranging from bacteria to humans. (biomedcentral.com)

The CDK9/cyclin-K complex has also a kinase activity towards CTD of RNAP II and can substitute for CDK9/cyclin-T P-TEFb in vitro (PubMed:21127351). (wuxibiortus.com)
ATF-2 interacts with a variety of viral oncoproteins and cellular tumor suppressors and is a target of the SAPK/JNK and p38 MAP kinase signaling pathways. (thermofisher.com)
Insulin signaling affects multiple downstream pathways including mitogen-activated protein kinase (MAPK), which controls growth, sterol regulatory element-binding protein 1 (SREBP-1), which stimulates the synthesis of lipid and cholesterol as well as the family of Forkhead (FOXO) transcription factors regulating metabolism and autophagy [ 9 ] [ 10 ] . (encyclopedia.pub)
They further link to the activation of protein kinase C- (PKC-) induced generation of reactive oxygen species (ROS) [ 6 , 7 ], which further mediates the activation of downstream transcription factor nuclear factor kappa-light-chain enhancer of activated B cells (NF- κ B). Thus, the main treatments of DN refer to modulate glycemic and blood pressure through insulin and RAS inhibitors. (hindawi.com)
Although the senescent cells remain viable, they show typical changes with enlarged and flattened cell bodies, apoptosis resistance, increased activity of senescence-associated β -galactosidase (SA- β -gal), and upregulation of cyclin-dependent kinase (CDK) inhibitors including p16 INK4A , ARF proteins, and p21 [ 13 - 16 ]. (hindawi.com)
Mediates survival signals downstream of PI3 kinase and several growth factor receptors by phosphorylating AP0pototic proteins. (affbiotech.com)

In transient transfection assays, we found that addition of a histone deacetylase inhibitor, trichostatin A, strongly potentiated PR-driven transcription. (drugbank.com)
In addition pp32 a major component of the inhibitor of acetyltransferase (INHAT) complex (11) is involved in the maximal induction of ISG transcription (12). (techblessing.com)

These mutations lead to the production of a shortened histone acetyltransferase enzyme. (medlineplus.gov)
Cas9 activity, determined by insertion deletion mutations, was restored in Gal4P65-expressing cells, while no CRISPR enhancement was observed with UNC1999 treatment. (aip.org)
Scope includes mutations and abnormal protein expression. (cancerindex.org)
Mutations in the N-terminal zinc ribbon had little effect on transcription activity. (embl.de)

This study has found that Swr1, a Swi2/Snf2-related adenosine triphosphatase, is the catalytic core of a multisubunit, histone-variant exchanger that efficiently replaces conventional histone H2A with histone H2AZ in nucleosome arrays. (sdbonline.org)
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. (nih.gov)
The fundamental repeating unit of chromatin is the nucleosome which is composed of two copies each of the four core histones H2A, H2B, H3, and H4 around which about 147 bp of DNA is wrapped [ 1 ]. (biomedcentral.com)

A number of natural and synthetic molecules that inhibit histone acetyltransferase (HAT) or histone deacetylase (HDAC) activities have been described. (biomedcentral.com)

Here we report that human hnRNPA1 physically interacts with the nucleoprotein (NP) of IAV in mammalian cells at different time points of the viral replication cycle. (cdc.gov)

The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. (wikipedia.org)
In contrast, directing histone deacetylase-1 (HD1) to a promoter using the GAL4 DNA binding domain inhibited transcription. (drugbank.com)

Proteins associated with the Wnt/β-catenin pathway have been identified as SUMOylated substrates, and evidences suggested that the initiation and progression of cancers depended on the function of the SUMOylation ( 23 ). (frontiersin.org)
TAF-I and histone H1 double KD did not show the additive effect in ISG transcription suggesting that TAF-I and histone H1 may act on the same regulatory pathway to control ISG transcription. (techblessing.com)
This was followed by the contention that asthma is an autonomic dysfunction syndrome characterized by excess cholinergic and/or tachykinin pathway activity. (jci.org)

For instance, SIRT1 deacetylate lysine 310 of RelA/p65, the nuclear cleaved type of NF-kB that regulates NF-kB transcription activity and p53-reliant apoptosis [14], and CREB binding proteins (CBP), which is normally deacetylated to modulate CREB-dependent transcription. (monossabios.com)
Another SIRT1 substrate that regulates BMAL-CLOCK may be the TIMELESS proteins adversely, which can be a replisome complicated member [20]. (monossabios.com)

Coactivators, such as steroid receptor coactivator 1 (SRC-1A) and CREB (cAMP response element binding protein)-binding protein (CBP), are required for efficient steroid receptor transactivation. (drugbank.com)
The transcription factor ATF-2 (also called CRE-BP1) binds to both AP-1 and CRE DNA response elements and is a member of the ATF/CREB family of leucine zipper proteins. (thermofisher.com)

Acetylases are now known to modify a variety of proteins, including transcription factors, nuclear import factors and alpha-tubulin. (embl-heidelberg.de)
Unlike sequence-specific transcription factors, epigenetic regulators do not necessarily bind DNA at consensus sequences, but still achieve reproducible target binding in a manner that is cell and maturation-type specific. (frontiersin.org)

Tumor protein p53, a nuclear protein, plays an essential role in the regulation of cell cycle, specifically in the transition from G0 to G1. (affbiotech.cn)

E7 from human papil omavirus type 16 cooperate to cancer: the European prospective investigation into doi:10.1093/carcin/bgp321 PMID:20047954 target the PDZ protein Na/H exchange regulatory cancer and nutrition study. (who.int)

Non (protein)-coding RNAs are the most abundant transcriptional products of the coding genome, and comprise several different classes of molecules with unique lengths, conformations and targets. (frontiersin.org)
This table lists all participants of the complex (proteins, small molecules, nucleic acids, etc.) and their respective stoichiometry. (yeastgenome.org)
Drug-like molecules that can reprogram selected histone PTMs in tumour cells are therefore of interest as potential cancer chemopreventive agents. (biomedcentral.com)

8-11 Recently, several groups have investigated the impact of chromatin states on the binding and cutting of DNA by Cas9 guide RNA complexes (Cas9/gRNA). (aip.org)

There are two regions of observed Smad2/3 binding in control embryos, schematized in blue. (xenbase.org)
Several regions in the MLL N-terminus are critical for the activity of MLL-fusions. (nature.com)
To determine the reason for this species specificity, chimeras were constructed linking three structurally independent regions of the TFIIS proteins from yeast and human cells. (embl.de)

ETS-1 and ETS-2 mRNA and protein levels were much higher in the ocular tissues of Tyrp-1-TAg mice than in control ocular tissues from wild-type mice. (molvis.org)
Conversely, hnRNPA1 overexpression reduced NP expression at the mRNA and protein levels and impeded virus replication by (60.70%), suggesting antagonistic function. (cdc.gov)

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. (wikipedia.org)
Yeast domain II alone is able to bind yeast RNA polymerase II with the same affinity as the full-length TFIIS protein, and this domain was expected to confer the species selectivity. (embl.de)

In summary, although garcinol and curcumin can both inhibit histone acetyltransferase activities, our results show that these compounds have differential effects on cancer cells in culture. (biomedcentral.com)

Our results demonstrate the use of DNA-binding, activator-associated fusion proteins as an effective method to enhance Cas9 editing within polycomb-repressed chromatin. (aip.org)
MLL-fusion proteins are found in acute lymphoblastic leukemia (ALL) and acute myeloid leukemia (AML) and are often associated with adverse prognosis, particularly in pediatric patients 3 . (nature.com)
It has therefore been proposed that the oncogenic activity of MLL-fusion proteins depends on chromatin targeting functions exerted by the MLL N-terminus in combination with other functional properties encoded by the fusion partners 6 . (nature.com)
For instance, the CxxC-domain is essential for DNA binding of MLL-fusion proteins 7 . (nature.com)
However, it is unclear whether these molecular mechanisms pertain to the entire family of MLL-fusions or if they specifically affect the leukemogenicity of isolated MLL-fusion proteins. (nature.com)

1996. Id of individual protein conserved using the fungus putative adaptors ADA2 and GCN5 functionally. (isme-la2019.org)

METHODS: After treatment with RDS 3337, cell lysates were analyzed for autophagy and apoptosis-related proteins by Western blot. (bvsalud.org)

In most eukaryotes, two classes of histones have been described: (1) replicative or canonical histones, expressed only during the S phase of the cell cycle, which are assembled onto chromatin in a DNA replication-dependent (RD) manner, and (2) variant histones, which differ in their primary amino acid sequences, are expressed throughout the cell cycle, and can be deposited in a replication-independent (RI) fashion [ 2 ]. (biomedcentral.com)

From the 1598 potential SIRT1 substrates in the individual acetylome [11,12], one of the most abundant SIRT1 histone substrate is certainly lysine 16 of histone H4, a marker of energetic transcription. (monossabios.com)

Despite its conservativeness, however, the three-dimensional structure of AEG-1 has not been solved, and the functional domains of the protein are not clearly defined. (biomedcentral.com)

Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and hence cause the loss of tumor suppressor activity. (affbiotech.cn)
In this study, we have characterized the biological activities of an amino acid (AA) derivate of GlcN, called DCF001, in the growth and chondrogenic induction of circulating multipotent stem cells (CMCs) after priming with tumor necrosis factor-alpha (TNFα), a pleiotropic cytokine commonly expressed in chronic inflammatory joint diseases. (bvsalud.org)
NK activation was evaluated, and its tumoricidal activity was assessed using in vitro and in vivo tumor models. (weeksmd.com)
In addition, NK cells stimulated with lunasin plus cytokines displayed higher tumoricidal activity than those stimulated with cytokines alone using in vitro and in vivo tumor models. (weeksmd.com)

The Sir2 catalytic domain, which is shared among all sirtuins, consists of two distinct domains that bind NAD and the acetyl-lysine substrate, respectively. (biomedcentral.com)

Nearly all known histone-acetyltransferase (HAT)-associated transcriptional co-activators contain bromodomains, which are approximately 110-amino-acid modules found in many chromatin-associated proteins. (embl-heidelberg.de)

Anatomy15

Organisms9

Diseases3

Chemicals and Drugs91

Analytical, Diagnostic and Therapeutic Techniques and Equipment17

Phenomena and Processes60

Disciplines and Occupations1

Information Science3

Acetylation19

Reducing transcription1

Gene31

Nucleosomes7

Chromatin structure6

Highly conserved5

Enzymes5

Subunit3

Acetylates1

Modification5

Variant histone1

Nucleosomal histones1

Lysine residues1

Residues1

Specific histone1

Phosphorylation5

Ligand3

Regulate6

Transcriptionally active3

Genes9

P3002

Interactions3

Modifications5

Deacetylase activity2

Inhibition1

Eukaryotic2

Genomic5

Repression4

Deacetylases1

Kinase6

Inhibitor2

Mutations4

Nucleosome3

HDAC1

Physically interacts1

Promoter2

Pathway3

Regulates2

CREB2

Transcription factors2

Nuclear1

Regulatory1

Molecules3

Complexes1

Regions3

MRNA and protein levels2

Polymerase2

Inhibit1

Fusion proteins5

Functionally1

Apoptosis1

Eukaryotes1

Substrates1

Functional1

Tumor4

Distinct1

Amino acid1