Interaction cell biological assay. Medical search. Frequent questions

Fusion Proteins offer an important biological assay for direct protein-to-protein interactions. (thermofisher.com)
The foundations for which ligand binding assay have been built are a result of Karl Landsteiner, in 1945, and his work on immunization of animals through the production of antibodies for certain proteins. (wikipedia.org)
Our lab is also interested in extracellular vesicles such as exosomes and microvesicles as vehicles for the transfer of misfolded proteins between cells. (edu.au)
We are interested in investigating the mechanisms, of how these proteins are packaged into these vesicles and also how they transfer their contents between cells. (edu.au)
LA JOLLA-Salk scientists have developed a new high-throughput technique to determine which proteins in a cell interact with each other. (salk.edu)
A new mapping method let researchers discover new links (gray lines) between two groups of plant proteins (yellow and blue) that have a common structure (the BBX domain), suggesting many different combinations of interactions, rather than a few, are involved in coordinating cellular programs like flowering time and circadian rhythm. (salk.edu)
The interactome of a cell, like a map of social networks, lets scientists see who's working with who in the world of proteins. (salk.edu)
Until now, researchers have typically relied on standard high-throughput yeast two-hybrid (Y2H) assays to determine the interactions between proteins. (salk.edu)
But finding all the interactions between, for instance, 1,000 proteins, would require 1000 separate experiments to screen once for each bait's interaction partners. (salk.edu)
The genes for two proteins, each on their own circle of DNA, are added to the same cell. (salk.edu)
If the proteins of interest interact inside the cell, a gene called Cre is activated. (salk.edu)
The team can generate a massive library of yeast cells-each containing different pairs of proteins by introducing random combinations of genes on circular DNA called plasmids. (salk.edu)
When cells are positive for a protein interaction, the researchers can use genetic sequencing to figure out what the two proteins interacting are, using new high-throughput DNA sequencing technologies similar to those used for human genome sequencing. (salk.edu)
This way, they're no longer limited to testing one "bait" protein at a time, but could test the interactions between all the proteins in a library at once. (salk.edu)
When you take 1,800 proteins and test the interactions among them, that's nearly 4 million combinations," says Ecker. (salk.edu)
They revealed more than 8,000 interactions among those proteins tested, giving them new insight into which Arabidopsis transcription factors interact with each other. (salk.edu)
The course maintains stock mRNAs for targeting fluorescent proteins to specific structures for studying cell shape and cytoskeletal dynamics but students are encouraged to bring or suggest additional tools, including fluorescent biosensors, tension-sensors, etc. (cshl.edu)
Cytokines are small signaling proteins that are present in serum and cell secretomes at concentrations in the pg/mL or ng/mL range. (illinois.edu)
Lipid research encompasses the large-scale profiling of cellular lipids, the quantification of specific lipids in biological samples and the documentation of the interactions between lipids and other lipids, proteins and metabolites within the body. (sanbio.nl)
In AIM2, we will identify bona fide RG4-binding proteins using a novel approach based on proteomic/biochemical analysis of interactions between the 7-deazaguanine RNA derivatives and proposed binding factors. (grantome.com)
Here we used an affinity-purification mass spectrometry-based (AP-MS) approach to identify novel and particularly intracellular sGAG-interacting proteins in human bone marrow stromal cells (hBMSC). (degruyter.com)
Enrichment analysis for protein localization showed that mainly intracellular and cell-associated interacting proteins were identified. (degruyter.com)
Ceramide generated in late endosomal compartments is recognized as a potent regulator of cell signaling, but its molecular interactions with late endosomal transmembrane proteins have not been studied in depth. (sphingolipidclub.com)
The results have shown that DDB2 Wt recognize and repair the UV-induced lesions in plasmidic DNA transfected in the cells, whereas a delay in these processes were observed in the presence of DDB2 PCNA- , as also confirmed by the different extent of co-localization of DDB2 Wt and some NER proteins (such as XPG), vs the DDB2 mutant form. (biomedcentral.com)
Replacements L38E and L41E, involving charge acquisition at residues predicted to contribute to the hydrophobic interface, reduced the dimerization signal in the protein ligation assay and prevented the detection of dimer/multimer species in both transiently expressed 3A proteins and in synthetic peptides reproducing the N terminus of 3A. (asm.org)
The viral particle is composed of a protein capsid that contains a positive-sense RNA molecule of about 8,500 nucleotides that is infectious and encodes a single polyprotein, which is processed in infected cells by cis - and trans -acting viral proteases ( 55 ) to yield different polypeptide precursors and the mature viral proteins ( 9 , 62 ). (asm.org)
NS proteins are involved in crucial aspects of the viral cycle and pathogenesis, such as rearrangements of intracellular membranes required for endomembrane recruitment and the lysis of host cells ( 1 , 12 , 14 , 18 , 73 ). (asm.org)
in poliovirus (PV), the interaction between the RNA replication complex and intracellular membranes appears to be accomplished by proteins 3A and 2C, which have membrane-binding properties ( 11 , 60 ). (asm.org)
All the venomous species have a venom gland that synthesizes, stores and secretes a complex mixture of biological molecules including proteins. (nature.com)
Produced by a variety of cell types, extracellular vesicles (EVs) are able to transfer lipids, nucleic acids and proteins to recipient cells. (nature.com)
We used the technique of X-ray crystallography to show in atomic detail how KSHV utilizes KsBcl-2 to neutralize host cell death inducing Bcl-2 proteins, including Bid and Puma, to ward off premature death of an infected cell. (mdpi.com)
HIC offers orthogonal selectivity for the clearance of difficult process and product-related impurities such as aggregates, host cell proteins and endogenous and adventitious viruses. (bioprocessintl.com)
The ECM composed of hundred proteins is a dynamic network controlling the proliferation, adhesion, migration, polarity, differentiation, and apoptosis of the cells. (premiumbeautynews.com)
BMPR family of proteins is a valuable tool for studying ligand-receptor interactions, downstream signaling events, and determining therapeutic targets. (news-medical.net)
These proteins display interactions and are involved in the regulation of the cellular cytoskeleton. (biomedcentral.com)
These findings suggest that parasite proteins are able to inhibit erythroid cell growth by down-regulation of ezrin phosphorylation, leading to ineffective erythropoiesis ultimately resulting in severe malarial anaemia. (biomedcentral.com)
Many molecular pathways in eukaryotic cells are modulated by specific signaling proteins that are controlled, by phosphorylation and dephosphorylation, through the activity of kinase and phosphatase enzymes. (biomedcentral.com)
The regulation of complex and dynamic signal transduction proteins contributes to the destination of targeting proteins and the signal transduction of cell growth, and exposure to parasites can also influence signaling pathways. (biomedcentral.com)
This occurs through specific modulation of regulatory proteins during the host-pathogen interaction, especially proteins with roles in pathogenesis [ 27 ]. (biomedcentral.com)
We study the interaction between proteins in biological fluids as well as single proteins to nanomaterials of different kinds. (lu.se)
She also developed multiplex assays to quantify proteins associated with different diseases, which were then used to screen clinical patient cohorts. (lu.se)

Since then, Attana has developed proprietary label free biosensors for biochemical, crude, sera, and cell-based assays and the Attana Virus Analytics (AVA) platform, a proprietary in vitro diagnostics (IVD) tool. (cision.com)
Cellular assays, or cell-based assays, can be used in both biomedical research and drug-discovery screening applications to efficiently quantify cytotoxicity, biological activity, biochemical mechanisms and off-target interactions. (sigmaaldrich.com)
Unlike traditional biochemical assays, cell-based assays are more physiologically relevant and can assess compound characteristics simultaneously. (sigmaaldrich.com)
The second is the application of new tools to designing selective PPI inhibitors for clinically highly relevant signaling pathways and characterizing the biological activities of new inhibitors using biochemical and cell-based assays. (usf.edu)
Here we will establish biochemical, biophysical and cellular platforms to systematically dissect the biological importance of RNA G4s in cellular physiology. (grantome.com)
Biochemical assays indicated that SVEVs are functionally active, showing high metalloproteinase and fibrinogenolytic activity besides being cytotoxic against HUVEC cells. (nature.com)
This "renowned" matrix plays one of the essential roles of the physical and biomechanical properties of the dermis with this intricate network of extracellular macromolecules providing cells structural, bioactive molecules and biochemical support. (premiumbeautynews.com)

Specifically, despite the human body's endogenous receptors, hormones, and other neurotransmitters, pharmacologists utilize assays in order to create drugs that are selective, or mimic, the endogenously found cellular components. (wikipedia.org)
Accordingly, understanding the underlying mechanism of cellular uptake is an important step toward understanding the biological fate of nanoparticles, both the favorable and adverse aspects [1] . (materialstoday.com)
In addition to parameters such as size and shape, chemical functionalities on the surface seem to play a critical role in binding to cell membrane and subsequent cellular uptake [2] and [3] . (materialstoday.com)
Consequently, the widespread theory that the preferential cellular uptake of positively charged nanoparticles over neutral or negatively charged nanoparticles is due to favorable electrostatic interactions with cell membrane seems simplistic and reductive. (materialstoday.com)
The three key elements of the toxicity screening strategy are: Physicochemical Characteristics, In Vitro Assays (cellular and non-cellular), and In Vivo Assays. (cdc.gov)
Non-cellular assessment of nanoparticle durability, protein interactions, complement activation, and pro-oxidant activity is also considered. (cdc.gov)
Fields such as medical diagnostics and cellular biology would benefit greatly from a technology that enables rapid, quantitative and reproducible assays for many targets within a single sample. (illinois.edu)
The lab's efforts are mainly focused on the rational design, synthesis, and biological evaluation of drug-like small molecules that can modulate cellular signaling pathways with an emphasis on protein-protein interactions (PPIs) and target specificity. (usf.edu)
When psychosine was supplied to proliferating cells, cells underwent special type of mitosis, endomitosis, which sustains cellular content duplication without segregation, resulted in the giant and multiploid cell formation. (sphingolipidclub.com)
The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
In addition, fluorescence microscopy and spectroscopy based techniques allow us to quantitatively study the cellular dynamics of molecules and the kinetics of their interaction with high spatio-temporal resolution and ultimate, single-molecule sensitivity. (ki.se)

2 Lipids are major participants in the conversion of energy within cells, the transportation of substances, and cell growth, differentiation and apoptosis. (sanbio.nl)
Results indicated compound 18e as the hit compound to investigate the potential mechanism of action by apoptosis assay, virucidal activity test, and the time of addition assay. (uniss.it)

It has been shown that inflammation caused by oxidized low-density lipoprotein (ox-LDL) contributes to the occurrence and development of AS [ 1 ], which can promote vascular smooth muscle cell (VSMC) migration, proliferation, and transformation from a contractile to a synthetic phenotype [ 2 - 6 ]. (hindawi.com)
Several studies have shown that miRNAs play multiple roles in the phenotypic transformation, migration, and proliferation of VSMCs by inhibiting ERK1/2 activation [ 11 , 12 ], partly by regulating the tissue inhibitor of metalloproteinases (TIMPs)-MMPs and p21-cyclins interactions [ 13 - 18 ]. (hindawi.com)
Cell viability and proliferation assays can be effectively used to evaluate cell health. (sigmaaldrich.com)
Cell viability and proliferation rate are often assessed using dyes such as trypan blue or Calcein-AM, or with fluorescent readouts. (sigmaaldrich.com)
Tier 1 evaluations include markers of inflammation, oxidant stress, and cell proliferation in portal-of-entry and selected remote organs and tissues. (cdc.gov)
Jurkat T cells encapsulated with a double layer of PSS-GNR/PAH (PSS-GNR/PAH@Jurkat) showed the highest rate of cell proliferation when compared to 24-h encapsulated cells. (springer.com)
Cancerous cells undergo major changes in lipid metabolism, meaning that lipids play a major role in the proliferation of cancerous tumours. (sanbio.nl)
Transfection with candidate miRNAs mimics significantly decreased neuroblastoma cells proliferation rate. (oncotarget.com)
Many viruses, including Kaposi Sarcoma Herpesvirus (KSHV), have evolved sophisticated countermeasures to extend the life of an infected host cell to support establishment of infection and virus proliferation. (mdpi.com)
We also show that in addition to Bid and Puma, KsBcl-2 is able to engage with a broad range of other host cell death inducers, thus making it a potent enabler of viral infection and proliferation. (mdpi.com)
In previously reports , Plasmodium vivax was found to be able to directly inhibited erythroid cell proliferation and differentiation. (biomedcentral.com)
Moreover, in vitro cultures of erythroid cells derived from haematopoietic stem cells has demonstrated that P. vivax is able to directly inhibit erythroid cell proliferation and differentiation [ 22 ]. (biomedcentral.com)

We are also using high-resolution cryo-electron microscopy to study the structure of exosomes isolated from neuronal cells and characterise their protein and RNA content using high throughput sequencing and proteomics techniques. (edu.au)
Discover a high throughput assay using organoids and Millicell ® Microwell plates. (sigmaaldrich.com)
Our high throughput assay protocol evaluates the killing capacity to colorectal tumor spheroids and enables patient-specific assays. (sigmaaldrich.com)

For instance, xMAP multiplex assay provides a platform for cancer biomarker assays, which are compatible with plasma, serum, cultured cells, and other biological samples. (alliedmarketresearch.com)
In 2007 she joined a biotech company in Hungary as a researcher and took part in the development of immune-based multiplex assays in the field of life sciences and healthcare. (lu.se)

Extracellular vesicles (EV) are released by cells and include exosomes, microvesicles and microparticles. (edu.au)
During cell invasion, cells can modulate the environment as they migrate through the structure of the extracellular matrix (ECM). (sigmaaldrich.com)

Attana was founded in 2002 with the vision of in vitro characterization of molecular interactions mimicking in vivo conditions. (cision.com)
Our novel blood brain barrier model allows direct cell-to-cell interactions, closely modeling the in vivo properties. (sigmaaldrich.com)
In vitro techniques allow specific biological and mechanistic pathways to be isolated and tested under controlled conditions, in ways that are not feasible in in vivo tests. (cdc.gov)
Tier 1 in vivo assays are proposed for pulmonary, oral, skin and injection exposures, and Tier 2 evaluations for pulmonary exposures are also proposed. (cdc.gov)
The drug discovery process requires that upwards of 10,000 molecules be screened for each new lead compound developed and relies upon an expensive and time consuming combination of in vitro cell culture-based and in vivo whole animal-based models to identify, validate, and ensure the safety of any resulting potential therapeutic agents. (inknowvation.com)
Finally, the in vitro model we have described should serve as a useful system to investigate the complex interactions that appear to operate in vivo within the biofilm-antifungal interphase. (who.int)
Testing the biological significance of RG4s requires proving that RG4s exist in vivo. (grantome.com)
To understand the physiological significance of HS-induced RAGE oligomerization in vivo, we generated RAGE knock-in mice ( Ager AHA/AHA ) by introducing point mutations to specifically disrupt HS-RAGE interaction. (elifesciences.org)
The in-vitro or ex-vivo assays can target the various biological mechanisms providing a limitless opportunities of claims support. (premiumbeautynews.com)
To understand the fundamental connections between particle properties and human and environmental toxicology we study toxicological effects on all levels, from single cells to ecosystems using various in-vitro, ex-vivo, and in-vivo methods. (lu.se)

The ability to visualize these protein-protein interactions in unmodified cells under endogenous expression and at very low levels is a fundamental advance that allows greater insight into basic biology, pathways, spatial phenomena, and potential therapeutic targets in those pathways," said Josef Zihlmann, vice president at Sigma Life Science. (genengnews.com)
Though many traditional sensing technologies for biomarker analysis and fundamental biological studies (i.e. enzyme-linked immunosorbent assays, real-time polymerase chain reaction, etc.) rely on single-parameter measurements, it has become increasingly clear that the inherent complexity of many human illnesses and pathways necessitates quantitative and multiparameter analysis of biological samples. (illinois.edu)
Today, lipid research has started to map the lipidome of human cells and describe lipid biological pathways. (sanbio.nl)
We found some articles related to environmental interactions with inherited genotype and others on environmental exposures that damage genetic material and initiate molecular pathways of pathogenesis. (cdc.gov)

Historically, ligand binding assay techniques were used extensively to quantify hormone or hormone receptor concentrations in plasma or in tissue. (wikipedia.org)
Together with labs at EMBL Heidelberg, ETH Zurich, Medical Universities of Vienna, Charite and Vienna University of Technology, Prof. Enrico Klotzsch's lab has developed a platform, which allows to quantify the magnitude and direction of traction forces exerted locally by the T cell fingers and at the same time measuring the overall T cell activation status. (hu-berlin.de)
These assays may be used to quantify and to assess the health of mammalian cell lines, primary cells and stem cells. (sigmaaldrich.com)

RORγ is activated by multiple endogenous ligands and controls the expression of genes involved in immune cell differentiation and function. (stratech.co.uk)
RORγ plays a critical role in the differentiation and function of immune cells, including T-helper 17 (Th17) cells. (stratech.co.uk)
RORγ has several isoforms, including RORγt, which is specifically expressed in Th17 cells and plays a critical role in Th17 cell differentiation and function. (stratech.co.uk)
RORγt is essential for the development of Th17 cells, and its genetic deletion or inhibition has been shown to reduce Th17 cell differentiation and disease severity in animal models of autoimmune diseases. (stratech.co.uk)

INDIGO's RAR-related Orphan Receptor Gamma (RORγ) assay kit is an all-inclusive firefly luciferase reporter assay system that includes, in addition to RORγ Reporter Cells, two optimized media for use during cell culture and (optionally) in diluting the test samples, a reference inverse-agonist, Luciferase Detection Reagent, a cell culture-ready assay plate, and a detailed protocol. (stratech.co.uk)
However, with existing bioluminescent reporter technology, this is simply not possible because the current market of bioluminescent reporter cells being applied toward toxicology screening relies upon a firefly luciferase gene construct that must be provided with a chemical substrate to activate its light emission response, resulting in only marginally informative single time point snapshots of potential toxicological interactions. (inknowvation.com)

The 3′UTR-binding sequence site of miR-128-5p on the p21 gene was predicted and assessed by luciferase assays. (hindawi.com)
Our goal is for each student to design a set of experiments focusing on their gene or biological interest. (cshl.edu)
To explore work on gene-environment interaction (GxE) at the Centers for Disease Control and Prevention (CDC) and the Agency for Toxic Substances and Disease Registry (ATSDR), we examined the CDC-Authored Genomics and Precision Health Publications Database (CDC-Authored GPHPD) , which includes articles published by CDC/ATSDR authors since 2011. (cdc.gov)
The CDC-Authored GPHPD was searched using the terms "environment," "gene environment," "gene environment interaction," and others* returning 54 unique articles, of which 15 were excluded because their primary focus was on other topics. (cdc.gov)

These results are complemented by cytotoxicity assays, thus providing insights into the biological effects of these particles on cells. (cipsm.de)

Cell based angiogenesis assays to analyze new blood vessel formation for applications of cancer research, tissue regeneration and vascular biology. (sigmaaldrich.com)
utilized a cell-free immunoprecipitation assay to analyze the neutralizing potential of the mouse monoclonal antibody 3F6 to hinder the BMPR2-ECD ligand-binding activity. (news-medical.net)

Lipidomic techniques are highly utilised for mechanistic assays, risk prediction, and monitoring treatment efficacy. (sanbio.nl)

Sigma® Life Science, Sigma-Aldrich's biological products and services business, signed an agreement to distribute worldwide Olink Bioscience's Duolink® In Situ products, which present a new method for imaging and measuring protein-protein interactions in unmodified cells. (genengnews.com)
According to the company, the PLA® technology allows Olink's Duolink In Situ products to quickly image the locations of single protein-protein interactions regardless of strength, measure protein expression levels, and identify relative changes in post-translational modification events. (genengnews.com)
Fluorescent detection probes bind the several hundred-fold amplified DNA at high density, allowing visualization of single protein-protein interactions inside an intact cell using a standard fluorescence microscope. (genengnews.com)
FLIM-FRET analysis of protein-protein interactions showed that PLIN5 S155 phosphorylation regulates PLIN5 interaction with adipose triglyceride lipase at the lipid droplet, but not with α-β hydrolase domain-containing 5. (uci.edu)

In this work, FMDV 3A homodimerization was evidenced by an in situ protein fluorescent ligation assay. (asm.org)

Brain tumor cells grow chaotically and destroy this anisotropy. (bvsalud.org)
Metastasis is the cumulative result of multiple changes in tumor cells. (sigmaaldrich.com)
Explore tumor metastasis employing various cell migration and invasion assays like Boyden chamber assay, Millicell® µ-Migration Assay and in vitro scratch assay. (sigmaaldrich.com)
This assay is used as a tool for in vitro analysis to study the dynamic interactions between cancer cells & immune cells, intricate tumor microenvironment, perceiving immunoreactivity in a multitude of cell-based assays to predict mode of action, and pharmacodynamics. (alliedmarketresearch.com)
Neuroblastoma is a neuroectodermal tumor that originates from precursor cells of the sympathetic nervous system. (oncotarget.com)
We report that cten binds to another tumor suppressor, deleted in liver cancer 1 (DLC-1), and the SH2 domain of cten is responsible for the interaction. (rupress.org)

A ligand binding assay (LBA) is an assay, or an analytic procedure, which relies on the binding of ligand molecules to receptors, antibodies or other macromolecules. (wikipedia.org)
Ligand binding assays provide a measure of the interactions that occur between two molecules, such as protein-bindings, as well as the degree of affinity (weak, strong, or no connection) for which the reactants bind together. (wikipedia.org)
The technology behind our work involves detecting a disease signature of RNA molecules called microRNA which can be isolated from small biological vesicles called 'exosomes' that travel in the bloodstream. (edu.au)
Study on- and off-rates (k on , k off ) of interactions between aptamers and target molecules. (2bind.com)
Though this technology can be used to detect a variety of molecules, this dissertation establishes the utility of microring resonator chips for multiparameter analysis of several challenging protein targets in cell cultures, human blood sera, and other clinical samples such as cerebrospinal fluid. (illinois.edu)
This photonic sensing modality is based on the interaction of the propagating evanescent field (that extends outside of the microring) with molecules near the ring surface. (illinois.edu)

We thank members of Professor Timo Otonkoski's group for advice and help in carrying out the insulin secretion assays. (lu.se)

Aptamer developers benefit from our extensive experience in aptamer characterization and our derived broad portfolio of suitable biophysical assays. (2bind.com)

Novel microscopy methods unravel the mechanical interaction of T-cells with small particles. (hu-berlin.de)

By virtue of their partially hydrophobic nature, lipids are the physical foundation of all living systems, providing the lipid bilayer cell wall that separates living cells from their environment. (sanbio.nl)
In Krabbe's disease patient cells, GalCer is the primary lipid species accumulated, its de-N-acylated psychosine is also accumulated and psychosine was suggested as a cause of toxicity. (sphingolipidclub.com)
Membrane glycosphingolipid (GSL) expression positively and sphingomyelin (SM) negatively correlated with multiploid cell formation, indicating the importance of lipid environment in the psychosine-mediated endomitosis. (sphingolipidclub.com)
Moreover, endomitotic cell cycles could be modulated by the lipid composition of the membrane among GSL, SM and phosphoinositides. (sphingolipidclub.com)

As a direct result of these monumental findings, researchers have continued the advancement of ligand binding assays in many facets in the fields of biology, chemistry, and the like. (wikipedia.org)
The Hill lab uses a combination of Biochemistry, Molecular and Cell Biology to investigate these diseases. (edu.au)
Xenopus is remarkable for studying development and disease, including birth defects, cancer, and stem cell biology. (cshl.edu)
Graduate students in the Ji lab expect to receive training in rational drug design, synthetic organic chemistry, medicinal chemistry, chemical biology, and molecular and cell biology. (usf.edu)
G4 structural and functional studies have largely focused on DNA G4s, and the number of biological functions assigned to these motifs has grown rapidly since the discovery of their involvement in telomere biology. (grantome.com)
Support from the Swedish National Infrastructure for Biological Mass Spectrometry (BioMS) and the SciLifeLab, Integrated Structural Biology (ISB) platform, is gratefully acknowledged. (lu.se)
We thank Professor Ramanujan Hegde (MRC Laboratory of Molecular Biology, Cambridge, UK) for the kind gift of anti‐TRAPα antibody and Professor Peter Arvan (University of Michigan Medical School, Ann Arbor, MI, USA) for the kind gift of INS‐1 832/13 cells. (lu.se)

The order shows a market acceptance for our technology and capability to characterize interactions with cells, tissues, viruses etc it also provides a value of our application knowledge and the advises we can provide. (cision.com)
Characterize aptamer target interactions with respect to their thermodynamics parameters including enthalpy (ΔH), free enthalpy of binding (ΔG), entropy (ΔS), and stoichiometry using ITC. (2bind.com)
In AIM1, we will determine and characterize the fraction of the human transcriptome that contains putative stress-responsive RG4s in living cells. (grantome.com)
Many technologies are used to characterize biological products, manufacturing processes, and raw materials. (bioprocessintl.com)

Antibody 3F6 reduces BMPR2-ECD ligand-binding activity in a modified immunoprecipitation assay. (news-medical.net)

A scratch wound-healing assay was used to detect the migration of VSMCs, and immunocytochemistry and western blotting for SM22ɑ were used to evaluate phenotypic transformation. (hindawi.com)
Cell culture protocol: the endothelial cell transwell migration and invasion assay used to study angiogenesis and cancer cell metastasis. (sigmaaldrich.com)
Cell migration is the movement of cells in response to specific external signals, including chemical and mechanical stimuli. (sigmaaldrich.com)
Cell migration and invasion can be evaluated in vitro with widely-used membrane-based migration systems such as the Boyden Chamber assay. (sigmaaldrich.com)
Cell migration and invasion kits are oftenn used in cancer research to study cell motility and invasiveness in response to chemoattractant gradients. (sigmaaldrich.com)

3. Describe the theoretical background behind specialized fluorescence based methodologies for studying molecular interactions in live cells. (ki.se)
The materials we make are niche polymers with tailor-made sites that facilitate selective molecular interactions. (lu.se)

Angiogenesis assays can be used to study both the induction and inhibition of angiogenesis in in vitro cell models. (sigmaaldrich.com)
This process is frustratingly exacerbated by an unfortunate dichotomy whereby the inexpensive in vitro cell culture systems used for tier 1 screening contribute to failures due to their inabiliy to model the complexity and parallel systems interaction inherent in whole animal models (which are responsible for up to 92% of new compound failures at the clinical level) and because they are not capable of demonstrating species-specific effects. (inknowvation.com)

There are numerous types of ligand binding assays, both radioactive and non-radioactive. (wikipedia.org)
As such, ligand binding assays are a superset of radiobinding assays, which are the conceptual inverse of radioimmunoassays (RIA). (wikipedia.org)
Some newer types are called "mix-and-measure" assays because they do not require separation of bound from unbound ligand. (wikipedia.org)
Ligand binding assays are used primarily in pharmacology for various demands. (wikipedia.org)
The ligand-binding assay methodology quantified the concentration of the hormone in the test material by comparing the effects of the test sample to the results of varying amounts of known protein (ligand). (wikipedia.org)
The first successful ligand binding assay was reported in 1960 by Rosalyn Sussman Yalow and Solomon Berson. (wikipedia.org)
Alternatively, test the influence of your aptamer on interactions between a target and its natural ligand. (2bind.com)
RORγ is known to activate transcription through ligand-dependent interactions with co-regulators and is involved in the development of secondary lymphoid tissues, autoimmune diseases, inflammatory diseases, the circadian rhythm, and metabolism homeostasis. (stratech.co.uk)

Mechanical forces play a vital role for many biological mechanisms, such as the sensation of infected cells by T-cells. (hu-berlin.de)
With respect to the mechanisms of RORs in cancer, previous studies on the regulatory mechanisms of RORs in cancer were mostly focused on immune cells and cytokines, but lately there have been investigations concentrating on RORs themselves. (stratech.co.uk)
Fluorescence microscopy and associated techniques are indispensable research tools for investigating molecular mechanisms of biological processes. (ki.se)

Our findings illustrate a key role of the EGLN-pVHL pathway in suppressing RIPK1 activation under normoxic conditions to promote cell survival and a model by which hypoxia promotes RIPK1 activation through modulating its proline hydroxylation to mediate cell death and inflammation in human diseases, independent of TNFR1. (nature.com)
Cell-based assay development for drug discovery can accelerate optimization of treatments for cancer and other angiogenesis-dependent diseases. (sigmaaldrich.com)
Th17 cells produce pro-inflammatory cytokines, such as interleukin-17 (IL-17), which contribute to tissue inflammation and damage in diseases like psoriasis, rheumatoid arthritis, and inflammatory bowel disease. (stratech.co.uk)
The transplantation of cells or tissues has been applied for treating various human diseases (Bhatia et al. (springer.com)
Therefore, cell encapsulation of T lymphocytes might help mitigate this problem and increase the efficiency of immunotherapy in many diseases. (springer.com)
As lipids have such critical biological roles, any deviations from their regular function can result in a variety of diseases. (sanbio.nl)
She has participated in and led several proteomic and multi-omic studies, which were primarily aimed at understanding the complex biological processes underlying development and heterogeneity of various diseases, such as Alzheimer´s disease and various cancers, as well as exploring possible new diagnostic and therapeutic options. (lu.se)
She is associated with the Swedish National Infrastructure for Biological Mass Spectrometry, and a member of the Working group on Protein Misfolding and Aggregation Diseases in B/D HPP. (lu.se)

Last week four different clinical groups at one of our key customers were trained in performing biological relevant assays using primary cells. (cision.com)
The goal is to apply clinical samples for detailed interaction analysis to cure cancer. (cision.com)
Thus, inhibiting HOX-PBX interaction represents a potential strategy to prevent these malignancies, as demonstrated by the patented peptide HTL001 that is being studied in clinical trials. (mdpi.com)
These assays are used in clinical trials, as they decrease the cost of research & manual labor and increase the efficiency of experiments and therapies. (alliedmarketresearch.com)
However, despite growing biological and clinical significance, little is known about the structural arrangement of this receptor or any of its family members. (uci.edu)

To clarify this issue, we decided to apply a transfection-based assay, named Host Cell Reactivation (HCR), to investigate DNA lesions removal efficacy in the presence of DDB2 Wt protein or DDB2 mutated one. (biomedcentral.com)

The investigation of nanoparticle/cell interactions is a crucial issue with regard to two fields: nanomedicine and nanotoxicology. (materialstoday.com)
It is commonly admitted that nanoparticles physico-chemical features are determining factors in nanoparticle/cell interactions and consequently influence cell behavior. (materialstoday.com)
There is a strong likelihood that biological activity of nanoparticle s will depend on physicochemical parameters not routinely considered in toxicity screening studies. (cdc.gov)

The proposed studies may elucidate molecular events underlying normal and pathological aspects of cell physiology, and identify events contributing to the tumorigenic or neurodegenerative changes. (grantome.com)

Although binding assays are simple, they fail to provide information on whether or not the compound being tested affects the target's function. (wikipedia.org)
Optimized cell-based assays deliver crucial information to confirm the efficiency of a drug or a compound in simple and progressively complex co-culture assays. (alliedmarketresearch.com)
The compound-treated cells were infected with SARS-CoV-2. (uni-wuerzburg.de)
This provides an uninterrupted stream of visual data over the lifetime of the reporter cell as it interacts and reacts to compound exposure at both its pre- and post-biotransformed states. (inknowvation.com)
To overcome these detractions and develop an improved tier 1 screening system that reduces the cost and time required for new compound evaluation, 490 BioTech proposes to develop a panel of multiple continuously bioluminescent human cell lines that will permit the simultaneous monitoring of each line to ascertain both the individual effects of compound treatment as well as the downstream effects of a compound's biotransformed metabolic breakdown products in real-time. (inknowvation.com)
in this study, compound 18e was proved to protect cells from viral infection. (uniss.it)
From the performed biological assays, compound 18e turned out to be non-cytotoxic as well as cell protective against CVB5 infection, with a mechanism of action ascribable to an interaction on the early phase of infection, by hijacking the viral attachment process. (uniss.it)

The immuno-oncology assay is a type of immunoassay primarily used for cancer diagnosis. (alliedmarketresearch.com)

However, their relevance for investigations of drug-drug and drug-organic anion (e.g., bile acid, steroid hormone) interactions at the transporter level remains to be established. (cancerindex.org)

Dr. Rezeli has 15 years of experience in biological mass-spectrometry. (lu.se)

red blood cell folate and serum folate will be evaluated using the World Health Organization-recommended microbiologic assay. (cdc.gov)

They investigated the binding interaction for insulin and an insulin-specific antibody, in addition to developing the first radioimmunoassay (RIA) for insulin. (wikipedia.org)

Cell 177 , 1094-1107 (2019). (nature.com)
The global immuno-oncology assay market accounted for $2,769 million in 2018, and is expected to reach $6,179 million by 2026, growing at a CAGR of 10.6% from 2019 to 2026. (alliedmarketresearch.com)

Attana has filed an international patent application with claims for methods for assessing the neutralizing potential of antibodies in biological samples. (cision.com)
In addition, students can combine these techniques with explant and transplant methods to simplify or test tissue level interactions. (cshl.edu)
In addition, they offer easier & faster analysis of cancer, and are automated as compared to conventional methods, thus fueling the immune-oncology assay market growth. (alliedmarketresearch.com)
Methods We treated cell cultures with BC/BL or defined single plant extracts, purified anthocyanins and astaxanthin in different concentrations and subsequently infected the cultures with the Measles virus (wild-type or vaccine strain Edmonston), Herpesvirus 1 or 8, or murine Cytomegalovirus. (uni-wuerzburg.de)
The MIPs are produced by various polymerization methods but in all cases specifi c interactions, molecular partitioning and polymer structure are key properties that we focus on. (lu.se)

In contrast, our substrate-free, autobioluminescent reporter cell lines emit light continuously and can modulate the output level of this signal in real time in response to cytotoxic interactions. (inknowvation.com)
With current in vitro screening assays now representing a $1.4B market with a predicted 12% annual growth rate, we believe we possess a product capable of significantly impacting the chemical/drug screening market and, here in particular, advancing our understanding of cytotoxic chemical biotransformations as they pertain to public health and consumer safety. (inknowvation.com)

This project will also investigate the role of modifiers in exosome biogenesis by altering their expression with RNAi, using a lentiviral delivery system in neuronal cells. (edu.au)
Xenopus is increasingly being used as imaging test-bed to investigate the roles of cytoskeleton and intracellular trafficking in cell biological and morphogenetic contexts. (cshl.edu)
In this work we investigate the interaction between endothelial cells and nanoparticles emitted by catalytic converters. (cipsm.de)

Cell 174 , 1477-1491.e19 (2018). (nature.com)

Using live-cell imaging and electron microscopy we clearly show that 46 nm platinum-decorated ceria nanoparticles can rapidly penetrate cell membranes and reach the cytosol. (cipsm.de)

Expertise and capability in functional genomics and DNA based assay design can be applied to numerous disciplines, research interests and diagnostic test development in the food or animal testing areas. (dcu.ie)
In AIM 3, we will use functional assays to determine the biological significance of RG4s in mRNA stability, localization and translation. (grantome.com)
We sought to identify PKA phosphorylation sites in PLIN5 and assess their functional relevance in cultured cells and the livers of mice. (uci.edu)

Focus on toxicological aspects of pesticide chemical interaction in drinking water contamination. (cdc.gov)

For example, T-cells (and other cells in our immune system) use mechanical forces to explore their environment, by pulling or pushing the other cells or tissue surfaces. (hu-berlin.de)
2000 ). The encapsulation of cells has been developed to avoid immune rejection in cell/tissue transplantation. (springer.com)
In this lecture, I will focus on several new roles of SphKs and Spns2 in regulation of immune cell trafficking, cancer progression, and pulmonary metastasis. (sphingolipidclub.com)

Expression of phosphorylation-defective PLIN5 S155A in Plin5 null cells resulted in decreased rates of lipolysis and triglyceride-derived fatty acid oxidation. (uci.edu)
Unexpectedly, the interaction between DLC-1 and the cten SH2 domain is independent of tyrosine phosphorylation of DLC-1. (rupress.org)

This dissertation describes the fabrication, characterization, development, and application of silicon photonic microring resonator technology to multiplexed protein measurements in a variety of biological systems. (illinois.edu)
This course is on advanced fluorescence microscopy imaging and spectroscopy techniques for quantitative characterization of molecular transport and interactions in cells. (ki.se)
Image analysis techniques for quantitative characterization of cell phenotypes (CellProfiler). (ki.se)
We study the effects on lung cell cultures using e. g. the NACIVT system and the array of characterization instruments of the aerosol lab in close collaboration with Occupational and Environmental Medicine and Lund Bioengineering and Regeneration. (lu.se)

Guidelines for various bioassays on the MultiScreen system, which makes it possible to carry out an assay from sample incubation to scintillation counting in a 96-well plate. (sigmaaldrich.com)
Scanning electron microscopy revealed that C. albicans formed a contiguous biofilm with tightly packed blastospores and occasional hyphae compared with C. parapsilosis and C. krusei, which developed confluent biofilms displaying structural heterogeneity and a lesser cell density, after 48 h of incubation on nutrient agar. (who.int)

When expressed as a recombinant protein in transfected cells, PV 3A cofractionates with endoplasmic reticulum markers ( 66 ), and its single transient expression can disrupt the secretory apparatus ( 23 ) and decrease major histocompatibility complex (MHC) class I expression ( 22 ). (asm.org)

In previous courses, we have guided students in the ablation of a wide variety of genes and helped them design suitable assays for their biological interests. (cshl.edu)

Consecutive pathway and cluster analysis led to the identification of biological processes, namely processes involving binding and processing of nucleic acids, LRP1-dependent endocytosis, and exosome formation. (degruyter.com)
RN1 inhibits Smad/Id-3 signaling pathway by blocking Gal-3 interaction with BMPRs. (news-medical.net)

This assay has the potential to begin to address questions about fundamental biological interactions that we haven't been able to address before. (salk.edu)
Studies using the frog Xenopus have made remarkable contributions to our understanding of fundamental processes such as cell cycle regulation, transcription, translation and many other topics. (cshl.edu)
cytokine quantitation is thus important in fundamental biological studies and diagnostics, and complex and overlapping cytokine roles make multiplexed measurements especially vital. (illinois.edu)

These forces help T-cells to discriminate between pathogenic invaders and the body's own cells, during the so-called antigen recognition process. (hu-berlin.de)
This procedure majorly uses the body's natural defense system to target and destroy cancer cells. (alliedmarketresearch.com)

Mitochondrial carriers catalyse the translocation of numerous metabolites across the inner mitochondrial membrane, playing a key role in different cell functions. (cancerindex.org)
Regarding cell membrane, surface charge and membrane potential are often confused and a sharper distinction should be done between the two as they refer to different concepts. (materialstoday.com)
This is why cell membrane is generally considered as globally negatively charged [5] . (materialstoday.com)
The uptake of nanoparticles by cells can be viewed as a two-step process: binding to the cell membrane and internalization. (materialstoday.com)
The accepted explanation lies in the fact that electrostatic interactions are favored with cell membrane that is negatively charged. (materialstoday.com)
Interestingly, no permanent membrane disruption or any other significant adverse effects on cells were observed. (cipsm.de)
Here we uncover structural motifs required for ceramide interaction with the four membrane spanning Lysosome Associated Protein Transmembrane 4B (LAPTM4B). (sphingolipidclub.com)
MARV assembles and buds from the host cell plasma where MARV matrix protein (mVP40) dimers associate with anionic lipids at the plasma membrane inner leaflet and undergo a dynamic and extensive self-oligomerization into the structural matrix layer. (uci.edu)
In cells, the majority of Sec61 is stably associated with a heterotetrameric membrane protein complex, the translocon-associated protein complex (TRAP), yet the mechanism by which TRAP assists in polypeptide translocation remains unknown. (lu.se)
Ribosome interactions anchor the Sec61/TRAP complex in a conformation that renders the ER membrane locally thinner by significantly curving its lumenal leaflet. (lu.se)

T helper 2 cells many important characteristics of chemical allergens are common to both contact and respiratory sensitizers. (cdc.gov)

These techniques bring new biological insight at an unprecedented rate and are of crucial importance for the development of life sciences. (ki.se)

1994. Genotoxic effects of the herbicides alachlor, atrazine, pendimethaline, and simazine in mammalian cells. (cdc.gov)

Intriguingly, RG4s appear predominantly unfolded in eukaryotic cells, whereas they are readily folded in vitro, suggesting that in cells RG4s are constitutively recognized and actively unfolded. (grantome.com)

This study aimed to identify the role of AA in oxidized low-density lipoprotein (ox-LDL)-induced vascular smooth muscle cell (VSMC) behaviours and to explore the effects of microRNAs (miRNAs). (hindawi.com)

Chemical inhibitor of nonapoptotic cell death with therapeutic potential for ischemic brain injury. (nature.com)
Mesenchymal stem cells (MSCs) are multipotent cells with immunomodulatory and neuroprotective potential. (bvsalud.org)
In this study, we aimed to assess the neuroprotective potential of MSCs from bone marrow (BM-MSCs) and stem cells from human exfoliated deciduous teeth (SHED) in the cuprizone model. (bvsalud.org)
These data provide promising results, demonstrating the potential use of encapsulated PSS-GNR/PAH@Jurkat to provide a more inert T cell population for immunotherapy application and other biomedical applications. (springer.com)
The potential spectrum of bioterrorism ranges from hoaxes and actual use of agents by individuals or groups against others, to state-sponsored terrorism that employs biological warfare (BW) agents and delivery systems that can produce mass casualties. (medscape.com)
Introduction The research of Hatch (1) has greatly increased our understand- ing of the use and potential limitations of biological markers for adverse reproductive effects. (cdc.gov)
Exposure to a potential fetal health hazard can be estimated through ecological assessment (e.g., testing the community water supply), questionnaires (e.g., classifying residents accord- ing to whether they drink bottled or tap water), or biological markers (e.g., testing for exposures to chemicals or solvents found in tap water). (cdc.gov)
Understanding the interactions between our environment and our genes and how they affect health outcomes offers a multitude of potential opportunities in public health. (cdc.gov)

4 For example, eicosanoids have been identified to play a role in cancerous cells' neoplastic transformations and blocking eicosanoid receptors can impede the inflammatory response of tumour cells. (sanbio.nl)
They reported that RN1 binding can potentially impede the interaction between Gal-3 and the BMPRs. (news-medical.net)
Biological weapons include any organism or toxin found in nature that can be used to incapacitate, kill, or otherwise impede an adversary. (medscape.com)

The diversity of proteoglycans and their high interaction with growth factors and their receptors provides structural basis for a multitude of biological functions. (premiumbeautynews.com)

Some recent results on the chemistry, formation, and biological activity of N-nitroso compounds. (cdc.gov)
In addition, we will have regular interactions with the permanent staff at Physical Chemistry and will get to meet students in their fi nal studies thereby enlarging our network and creating possibilities for future recruitment. (lu.se)

These latter usually have intracellular targets, therefore the nanodevice must be able to enter the cell to reach its objective and exert its effects [1] . (materialstoday.com)
Although sulfated GAGs (sGAGs) appear intracellularly, the knowledge about intracellular effects and putative interaction partners is scarce. (degruyter.com)
Respecting the preferentially intracellular localization of sGAG in vesicle-like structures, also the interaction data indicate sGAG-specific modulation of vesicle-based transport processes. (degruyter.com)

Anatomy20

Organisms10

Chemicals and Drugs26

Analytical, Diagnostic and Therapeutic Techniques and Equipment16

Phenomena and Processes38

Disciplines and Occupations2

Anthropology, Education, Sociology and Social Phenomena1

Information Science4

Proteins41

Biochemical7

Cellular11

Apoptosis2

Proliferation12

High-throughput3

Multiplex2

Extracellular2

Vivo10

Pathways4

Quantify3

Differentiation4

Firefly luciferase2

Gene4

Cytotoxicity1

Analyze2

Mechanistic1

Protein-protein inte4

Fluorescent1

Tumor6

Molecules6

Secretion1

Biophysical1

Particles1

Lipid4

Biology7

Characterize4

Immunoprecipitation1

Migration5

Molecular interactions2

Vitro cell2

Ligand8

Mechanisms3

Diseases8

Clinical5

Transfection1

Nanoparticle3

Tumorigenic1

Compound7

Immunoassay1

Steroid hormone1

Mass Spectrometry1

Serum1

Insulin1

20192

Methods5

Cytotoxic2

Investigate3

20181

Membranes1

Functional3

Toxicological1

Immune3

Phosphorylation2

Characterization4

Incubation2

Recombinant protein1

Suitable1

Pathway2

Fundamental3

Body's2

Membrane10

Characteristics1

Crucial1

Mammalian cells1

Eukaryotic cells1

Vascular smooth musc1

Potential8

Impede3

Multitude1

Chemistry2

Intracellular3