Interact histone acetyl acetyltransferase p300 cbp transcription factors. Medical search. Frequent questions

Histone acetylation is important in chromatin remodelling and gene activation. (embl-heidelberg.de)
Nearly all known histone-acetyltransferase (HAT)-associated transcriptional co-activators contain bromodomains, which are approximately 110-amino-acid modules found in many chromatin-associated proteins. (embl-heidelberg.de)
PCR2 targets genes that are developmentally regulated and catalyzes di- and histone tri-methylation, resulting in chromatin compaction and gene repression. (chicagobiomedicalconsortium.org)
Garcinol treatment alters expression of chromatin modifying enzymes in MCF7 cells, resulting in reprogramming of key histone and p53 PTMs and growth arrest, underscoring its potential as a cancer chemopreventive agent. (biomedcentral.com)
Enzymes that modify chromatin and associated proteins by the addition or removal of acetyl or methyl groups play a key role in genome regulation [ 1 ]. (biomedcentral.com)
These and other PTMs generate a combinatorial histone code that demarcates chromatin regions for transcription activation or repression [ 2 ]. (biomedcentral.com)
Thus, drug-like molecules that target chromatin modifying enzymes to reprogram selected histone PTMs in tumour cells may have potential as cancer chemopreventive agents. (biomedcentral.com)
Chromatin immunoprecipitation studies showed that HIPK2 was required in vivo for efficient p300/p53 co-recruitment onto apoptotic promoters and that both p53 modifications at Ser46 and Lys382 were necessary for p53 apoptotic transcription. (biomedcentral.com)
Acetylation of the N-terminal tails of histones "relaxes" the chromatin making it more accessible for binding by co-activating factors. (biomedcentral.com)
The canonical nucleosome is a fundamental unit of chromatin and consists of a protein octamer of two molecules each of histone H2A, H2B, H3 and H4, wrapped by 147 bp of genomic DNA. (aging-us.com)
Similarly, the recruitment of protein acetyltransferases positively correlates with histone hyperacetylation at active genes as shown by genome-wide chromatin immunoprecipitation (ChIP-Seq) studies of CBP and p300 in human T cells [ 3 ]. (aging-us.com)
Chromatin immunoprecipitation assay was performed to evaluate the histone modification of gene loci that are regulated by lunasin and cytokine. (weeksmd.com)
Chromatin assembly factor 1. (lookformedical.com)
A retinoblastoma-binding protein that is involved in CHROMATIN REMODELING, histone deacetylation, and repression of GENETIC TRANSCRIPTION. (lookformedical.com)
Although initially discovered as a retinoblastoma binding protein it has an affinity for core HISTONES and is a subunit of chromatin assembly factor-1 and polycomb repressive complex 2. (lookformedical.com)
This family of proteins includes a wide variety of classes, including CYCLIN-DEPENDENT KINASES, mitogen-activated kinases, CYCLINS, and PHOSPHOPROTEIN PHOSPHATASES as well as their putative substrates such as chromatin-associated proteins, CYTOSKELETAL PROTEINS, and TRANSCRIPTION FACTORS. (lookformedical.com)

Studies indicate that STAT2 requires a protein called interferon regulatory factor 9 (IRF9) to enter the nucleus. (wikipedia.org)
In addition, we show by a combination of structural and site-directed mutagenesis studies that bromodomains can interact specifically with acetylated lysine, making them the first known protein modules to do so. (embl-heidelberg.de)
Protein interacting with PcG histone methylation sites may provide sites for therapeutic intervention. (chicagobiomedicalconsortium.org)
Protein lysine acetyltransferases (HATs or PATs) acetylate histones and other proteins, and are principally modeled as transcriptional coactivators. (aging-us.com)
CREB binding protein (CBP, CREBBP) and its paralog p300 (EP300) constitute the KAT3 family of HATs in mammals, which has mostly unique sequence identity compared to other HAT families. (aging-us.com)
Nonetheless, CBP and p300 mutations in humans and mice show that these coactivators have important roles in development, physiology, and disease, possibly because CBP and p300 act as network "hubs" with more than 400 described protein interaction partners. (aging-us.com)
The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family. (cancerindex.org)
The herpes simplex virus 1 U(L)34 protein interacts with a cytoplasmic dynein intermediate chain and targets nuclear membrane. (uchicago.edu)
Herpes simplex virus 1 alpha regulatory protein ICP0 functionally interacts with cellular transcription factor BMAL1. (uchicago.edu)
The infected cell protein 0 of herpes simplex virus 1 dynamically interacts with proteasomes, binds and activates the cdc34 E2 ubiquitin-conjugating enzyme, and possesses in vitro E3 ubiquitin ligase activity. (uchicago.edu)
Here, we show that the lncRNA Panct1 regulates the transient recruitment of a putative X-chromosome-encoded protein A830080D01Rik, hereafter referred to as transient octamer binding factor 1 (TOBF1), to genomic sites resembling the canonical Oct-Sox motif. (ku.dk)
A histone chaperone protein that plays a role in the deposition of NUCLEOSOMES on newly synthesized DNA. (lookformedical.com)
A retinoblastoma-binding protein that has an affinity for core HISTONES. (lookformedical.com)
It is found as a subunit of protein complexes that are in involved in the enzymatic modification of histones including the Mi2 and Sin3 histone deacetylase complexes and the polycomb repressive complex 2. (lookformedical.com)

p53 acetylation at different C-terminus lysines including p300-mediated lysine-382 (Lys382) is also required for full activation of p53 transcriptional activity. (biomedcentral.com)
The purpose of the current study was to evaluate the interplay among HIPK2, p300, and p53 in p53 acetylation and apoptotic transcriptional activity in response to drug by using siRNA interference, p300 overexpression or deacetylase inhibitors, in cancer cells. (biomedcentral.com)
Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. (cancerindex.org)

There are three key parts of JAK-STAT signalling: Janus kinases (JAKs), signal transducer and activator of transcription proteins (STATs), and receptors (which bind the chemical signals). (wikipedia.org)
Acetylases are now known to modify a variety of proteins, including transcription factors, nuclear import factors and alpha-tubulin. (embl-heidelberg.de)
Post-translational modifications (PTMs) of histones and other proteins are perturbed in tumours. (biomedcentral.com)
Histone modifications act as signals that are 'read' by sensory proteins containing bromodomains, PHD fingers and other domains, many of which function as coregulators of DNA-binding transcription factors [ 4 ]. (biomedcentral.com)
These epigenetic mechanisms are mediated by chemical modifications of DNA, proteins (most commonly histones), and RNA. (activemotif.com.cn)
and nonhistone proteins (CHROMOSOMAL PROTEINS, NON-HISTONE) found within the nucleus of a cell. (lookformedical.com)

Cell viability/proliferation assays, cell cycle analysis by flow cytometry, immunodetection of specific histone and p53 acetylation marks, western blotting, siRNA and RT-qPCR. (biomedcentral.com)
CBP/p300 can also be important for transcription, but the recruitment of CBP/p300 and their associated histone acetylation marks do not absolutely correlate with a requirement for gene activation. (aging-us.com)

The nature of the recognition of acetyl-lysine by the P/CAF bromodomain is similar to that of acetyl-CoA by histone acetyltransferase. (embl-heidelberg.de)
Post-translational modification of histones, in particular the removal or addition of acetyl groups on ϵ-N-acetyl lysine residues, play an important role in epigenetic regulation of transcription. (biomedcentral.com)
Classification into the various types (designated histone I, histone II, etc.) is based on the relative amounts of arginine and lysine in each. (lookformedical.com)

Phenotype and gene expression changes are often surprisingly moderate or specific in yeast that harbor point mutations in the N-terminal tails of histones H2A, H2B, H3 and H4 [ 9 , 16 - 19 ]. (aging-us.com)

The recent identification of enzymes that regulate histone acetylation has revealed a broader use of this modification than was suspected previously. (embl-heidelberg.de)
The current experiments were designed to investigate the effects of Zyflamend on the expression of class I and II histone deacetylases, a family of enzymes known to be over expressed in a variety of cancers. (biomedcentral.com)
Histone deacetylases (HDACs) are a family of enzymes associated with cancer risk. (biomedcentral.com)
Enzymes that catalyze acyl group transfer from ACETYL-CoA to HISTONES forming CoA and acetyl-histones. (lookformedical.com)

In addition, Zyflamend up regulated the histone acetyl transferase complex CBP/p300, potentially contributing to the increase in histone 3 acetylation. (biomedcentral.com)

Interestingly trichostatin A (TSA), the inhibitor of histone deacetylase complexes (HDAC) did not have effect, suggesting that Sirt1 was the deacetylase involved in p53 deacetylation in HIPK2 knockdown. (biomedcentral.com)

Our results indicate that HIPK2 may regulate the balance between p53 acetylation and deacetylation, by stimulating on one hand co-recruitment of p300 and p53Lys382 on apoptotic promoters and on the other hand by inhibiting Sirt1 deacetylase activity. (biomedcentral.com)
Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. (cancerindex.org)

Analysis of CBP and p300 mutant mouse fibroblasts reveals CBP/p300 are together chiefly responsible for the global acetylation of histone H3 residues K18 and K27, and contribute to other locus-specific histone acetylation events. (aging-us.com)

In summary, although garcinol and curcumin can both inhibit histone acetyltransferase activities, our results show that these compounds have differential effects on cancer cells in culture. (biomedcentral.com)
A number of natural and synthetic molecules that inhibit histone acetyltransferase (HAT) or histone deacetylase (HDAC) activities have been described. (biomedcentral.com)
Our results suggest that the extracts of this polyherbal combination increase histone 3 acetylation, inhibit the expression of class I and class II histone deacetylases, increase the activation of CBP/p300 and inhibit cell proliferation, in part, by up regulating p21 expression. (biomedcentral.com)

Drug-like molecules that can reprogram selected histone PTMs in tumour cells are therefore of interest as potential cancer chemopreventive agents. (biomedcentral.com)
Histone PTMs are also critical for other genomic functions, such as DNA replication and induction of repair mechanisms at sites of DNA damage [ 3 ]. (biomedcentral.com)
In this context, numerous histone post-translational modifications (PTMs) have been described that include acetylation, phosphorylation, methylation, ubiquitylation and SUMOylation [ 1 ]. (aging-us.com)
Histone PTMs often correlate with the activity of the gene (or of a regulatory element such as an enhancer) that is in their proximity, suggesting a causal relationship. (aging-us.com)
The function of histone PTMs in mammals remains uncertain because the multiple genes encoding each canonical histone, renders in vivo mutational analysis unfeasible in most instances. (aging-us.com)
The difficulty of testing histone point mutations in mammals has therefore contributed to the uncertainty of whether canonical histone PTMs are correlative with gene expression or causal. (aging-us.com)
In baker's yeast, however, histone mutations can be easily made and numerous studies show that histone PTMs might not be as essential as their correlative behavior with transcription would suggest [ 9 - 14 ]. (aging-us.com)

CWR22Rv1 cells, a castrate-resistant prostate cancer cell line, were treated with Zyflamend and the expression of class I and II histone deacetylases, along with their downstream target the tumor suppressor gene p21, was investigated. (biomedcentral.com)
Zyflamend down-regulated the expression of all class I and II histone deacetylases where Chinese goldthread and baikal skullcap (two of its components) appear to be primarily responsible for these results. (biomedcentral.com)
Expression of the tumor suppressor gene p21, a known downstream target of histone deacetylases and CBP/p300, was increased by Zyflamend treatment and the effect on p21 was, in part, mediated through Erk1/2. (biomedcentral.com)

A histone chaperone that facilitates nucleosome assembly by mediating the formation of the histone octamer and its transfer to DNA. (lookformedical.com)

Thus, the bromodomain is functionally linked to the HAT activity of co-activators in the regulation of gene transcription. (embl-heidelberg.de)

Wild-type herpes simplex virus 1 blocks programmed cell death and release of cytochrome c but not the translocation of mitochondrial apoptosis-inducing factor to the nuclei of human embryonic lung fibroblasts. (uchicago.edu)

Relevant to the role of CBP/p300 as HATs, hyperacetylation of histone N-terminal tail lysines correlates strongly with active transcription [ 2 ]. (aging-us.com)

In addition, siRNA directed against p53 decreased MCP-1 transcription after TNF-α addition, directly confirming a crosstalk between p53 and MCP-1. (biomedcentral.com)

The He lab identified a core complex comprised of two subunits: METTL3 and METTL14, and an accessory factor WTAP, which mediates cellular m6A RNA methylation. (chicagobiomedicalconsortium.org)

Class I and II HDACs form complexes with multiple cofactors for activation where histones are a primary substrate [ 20 ] and have been targets for cancer therapies, including PrC [ 21 ]. (biomedcentral.com)

The pathway communicates information from chemical signals outside of a cell to the cell nucleus, resulting in the activation of genes through the process of transcription. (wikipedia.org)
These activated STATs form hetero- or homodimers, where the SH2 domain of each STAT binds the phosphorylated tyrosine of the opposite STAT, and the dimer then translocates to the cell nucleus to induce transcription of target genes. (wikipedia.org)
As a transcription factor that both activates and represses target genes p53 demands a highly complicated network to control and fine-tune responses to the different stress-signals encountered [ 1 ]. (biomedcentral.com)

In this study we assessed the effects of the phytocompounds garcinol and curcumin on histone and p53 modification in cancer cells, focussing on the breast tumour cell line MCF7. (biomedcentral.com)

This review sets out what we know about the broader substrate specificity and regulation of acetyl- ases and goes on to compare acetylation with the process of phosphorylation. (embl-heidelberg.de)

This article provides an introduction to the world of epigenetics, covers the history of this field in biology, discusses the factors and players that play a role in this process, and highlights which biological processes are influenced by epigenetic mechanisms. (activemotif.com.cn)

Although treatment with curcumin, garcinol or the garcinol derivative LTK-14 hampered MCF7 cell proliferation, differential effects of these compounds on histone modifications were observed. (biomedcentral.com)
In addition to its effects on histone modifications, garcinol was found to block CBP/p300-mediated acetylation of the C-terminal activation domain of p53, but resulted in enhanced acetylation of p53K120, and accumulation of p53 in the cytoplasmic compartment. (biomedcentral.com)

Activation of p53-mediated gene transcription is a critical cellular response to DNA damage and involves a phosphorylation-acetylation cascade of p53. (biomedcentral.com)

It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. (cancerindex.org)

These results reveal a novel role for HIPK2 in activating p53 apoptotic transcription. (biomedcentral.com)

Regulatory transcription factors (TFs) are encoded by approximately 10% of the human genome [ 1 ]. (biomedcentral.com)

It has histone deacetylase activity and represses transcription when tethered to a promoter. (cancerindex.org)

TOBF1 physically interacts with Panct1 and exhibits a cell-cycle-specific punctate localization in ESCs. (ku.dk)

Although studies in yeast show that many histone mutations cause modest or specific phenotypes, similar studies are impractical in mammals and it remains uncertain if histone acetylation is the primary physiological function for CBP/p300. (aging-us.com)
e.g. they bind selectively to DNA, stimulate transcription resulting in tissue-specific RNA synthesis and undergo specific changes in response to various hormones or phytomitogens. (lookformedical.com)

For instance, a systematic analysis of 486 different histone H3 and H4 mutations in yeast (where every residue was mutated at least one way) showed that only 11 of 79 N-terminal tail deletions resulted in lethality, a phenotype that also depends to some extent on strain background [ 9 ]. (aging-us.com)

DNA sequence) influences the extent to which CBP and p300 are necessary for transcription. (aging-us.com)

Is histone acetylation the most important physiological function for CBP and p300? (aging-us.com)

Exceptions to this limitation occur when analyzing histone variants that have few gene copies, or when assessing putative gain-of function histone mutations, such as those identified in pediatric glioblastoma and glioma (e.g. (aging-us.com)

We have previously reported that post-transplant lymphoma patients have an acquired deficiency of signal transducer and activator of transcription 4, which results in defective IFNÎ³ production during clinical immunotherapy. (weeksmd.com)

cdc2 cyclin-dependent kinase binds and phosphorylates herpes simplex virus 1 U(L)42 DNA synthesis processivity factor. (uchicago.edu)

In both cases, non-functional p53 leads to diminished MCP-1 transcription upon TNF-α treatment. (biomedcentral.com)

Anatomy17

Organisms9

Chemicals and Drugs127

Analytical, Diagnostic and Therapeutic Techniques and Equipment27

Phenomena and Processes75

Disciplines and Occupations1

Information Science3

Chromatin16

Protein14

Transcriptional3

Proteins6

Acetylation marks2

Lysine3

Tails of histones1

Enzymes4

Transferase1

Deacetylase complexes1

Deacetylation2

Residues1

Inhibit3

PTMs7

Deacetylases3

Octamer1

Functionally1

Fibroblasts1

Lysines1

SiRNA1

Methylation1

Complexes1

Genes3

Modification1

Substrate1

Epigenetic1

Modifications2

Cellular1

Regulation1

Role1

Regulatory1

Represses1

Cell1

Specific2

Yeast1

Sequence1

Important1

Function1

Results1

Synthesis1

Treatment1