• This document has been developed by CDC to give guidance to laboratories performing lymphocyte immunophenotyping assays in human immunodeficiency virus-infected persons. (cdc.gov)
  • Ensure optimal flow cytometer instrument performance by using Thermo Scientific™ Flow Cytometry beads for calibration, alignment, counting and instrument set-up to ensure flow and optical stability and for developing assays used for the simultaneous detection and quantitation of up to 24 different. (thermofisher.com)
  • Antibodies NovaFluor dye-conjugated antibodies Brilliant Ultra Violet and Brilliant Violet Antibodies Assays and reagents Beads for compensation, instrument controls, and standards 5 steps to. (thermofisher.com)
  • and specific protocols for organelle functional assays as well more pedestrian immunophenotyping. (typepad.com)
  • This makes FlowCytes the ideal cell controls to calibrate, standardize and ensure performance for instruments and assays. (slingshotbio.com)
  • Our biomarker controls are engineered with key surface antigen epitopes for standard immunophenotyping assays. (slingshotbio.com)
  • BD Biosciences multiomic instruments and reagents are designed to facilitate and support your single-cell multiomic research. (bdbiosciences.com)
  • Speak with your representative about including reagents as part of your instrument purchase to enable you to minimize the cost and risk of optimizing new high-parameter panels on your BD FACSymphony™ Cell Analyzer. (bdbiosciences.com)
  • Our high-parameter specialists will work with you to identify the optimal panel for your experimental design and instrument configuration and provide the reagents needed to get to the final rendition of your panel. (bdbiosciences.com)
  • Since the '90s immunophenotyping (staining cells with antibodies conjugated with fluorochromes and detection with flow cytometer) became the preferred method in diagnostics of haematological malignancies. (wikipedia.org)
  • A comprehensive range of probes, detection, ancillaries, and instruments for automated or manual ISH detection in fluorescence and brightfield applications. (leicabiosystems.com)
  • If muscle biopsy is performed, a moderately weak muscle should be selected with specimens frozen for cryostat sections to perform histologic and enzyme histochemical stains and immunocytochemistry for major histocompatibility complex (MHC) antigens, immunophenotyping of T cells, and detection of cytokines and complement. (medscape.com)
  • With protocols optimized for the MARS instruments, the kits provide an integrated cell isolation solution. (appliedcells.com)
  • Perform instrument QC protocols and maintain calibrations/tunings reports of cytometers. (stanford.edu)
  • The process of measuring the percentage of CD4+ T-lymphocytes in the whole blood sample is referred to as ``immunophenotyping by flow cytometry'' (9-14). (cdc.gov)
  • Total absolute cell count and percent lymphocyte values are usually obtained using the number of white blood cells and lymphocytes counted on an automated hematology instrument multiplied by the percentage of positivelystained cells measured on a flow cytometer. (beckman.com)
  • Immunophenotyping relies on detecting specific antigenic determinants on the surface of WBCs by antigen-specific monoclonal antibodies that have been labeled with a fluorescent dye or fluorochrome, such as phycoerythrin (PE) or fluorescein isothiocyanate (FITC). (cdc.gov)
  • Each BD fluorochrome optimized for flow cytometry has been conjugated to anti-human or mouse CD4 and is provided for PMT voltrations and instrument characterization. (bdbiosciences.com)
  • Immunophenotyping is the characterization of cells based on the expression of genes and combinations of genes. (bio-rad.com)
  • With these high-performance instruments, it's possible to use up to four lasers and 14 fluorescence channels. (excedr.com)
  • Panels, consisting of screening tube and supplementary characterisation tubes, are based on experiences and knowledge from literature but further optimised and tested in multiple research centers on large collection of samples impeaching on selection of fluorochromes and standardization of instrument settings and SOPs. (wikipedia.org)
  • BD is positioned with BD ® CS&T and BD ® FC Bead technology to enable instrument standardization simply from day to day, instrument to instrument, and lab to lab. (bdbiosciences.com)
  • Reference control-based instrument standardization is the next step for flow cytometry. (bdbiosciences.com)
  • T-cell numbers were derived from three measurements deter- attributable to the decrease in the number of T cells that bear mined with two different instruments, a hematology analyzer the CD4 receptor ( 5-9 ). (cdc.gov)
  • The sensitivity of FCM is about 10 −4 for MRD in AML whose immunophenotyping is obviously different from the normal cells, whereas the sensitivity is only 10 −3 when immunophenotyping is partially overlapped with normal cells [ 15 , 16 ]. (oncotarget.com)
  • The ability to stain live cells with a viability dye and preserve that staining pattern after fixation is critical for intracellular immunophenotyping. (thermofisher.com)
  • that describe dual-platform technology, a method in which absolute counts are derived from measurements obtained from two instruments--a flow cytometer and hematology analyzer. (cdc.gov)
  • This Standard (Indirect) Method, however, is associated with wide interlaboratory variation.1-4 Flow-Count Fluorospheres is an efficient alternative to the Standard (Indirect) Method for absolute counts and provides this measure directly using the single platform of flow cytometric immunophenotyping analysis. (beckman.com)
  • This high-end instrument allows polychromatic flow cytometry analysis up to 18 different colours (17 simultaneously). (edu.au)
  • Yoav is available to assist with reagent selection, experiment design, data analysis, protocol development, grant preparation, instrument training and operation and writing methods sections for publications. (sbpdiscovery.org)
  • These instruments purify and collect samples for additional analysis. (excedr.com)
  • Microspheres are small, non-biological beads that can be used as a (1) standard for flow cytometer instrument calibration, (2) reference for cell or particle size, and/or (3). (thermofisher.com)
  • Though there are many types of flow cytometers, benchtop instruments are the primary choice. (excedr.com)
  • Common sample types can be analyzed on a single cost-effective instrument. (atonarp.com)
  • As a Special Order Research Product (SORP), the BD FACSymphony™ A5 Cell Analyzer offers a choice of lasers from 26 different laser wavelengths to optimally configure your instrument for your specific research application. (bdbiosciences.com)
  • The powerful lasers and integrated optics from the company's CytoFLEX* instrument provide clear, accurate population resolution. (beckman.hk)
  • Configure your instrument to meet your research needs using traditional or unique wavelengths and power ratings. (bdbiosciences.com)
  • Under the agreement, Research Instruments will distribute BioSkryb's single-cell amplification products in Singapore and elsewhere in Southeast Asia. (genomeweb.com)
  • Our broad range of tissue processors means you can choose the right instrument for your laboratory's space, throughput, and workflow needs. (leicabiosystems.com)
  • The instrument features an ultra-quiet VPX electronics system that supports up to 50 high-performance detectors. (bdbiosciences.com)
  • The instrument imparts an electrical charge to molecules and converts the resultant flux of electrically charged ions into a proportional electrical current that a data system then reads. (atonarp.com)
  • The guidelines describe single-platform technology (SPT), a process in which absolute counts of lymphocyte subsets are measured from a single tube by a single instrument. (cdc.gov)
  • 10). To respond to the particular requirement data obtained from laboratory analyzers and re- of the accreditation standard for medical laborato- sults transferred into LIS, and between data en- ries, the accuracy of the entire process of data tered into LIS and then into HIS. (who.int)
  • Upon entry into the instrument, two nozzles descend onto each side of every spot, one to dispense wash buffer and the other to aspirate the buffer and debris away. (curiox.com)