• Preventing cell spreading by depolymerizing the actin cytoskeleton with Latrunculin B abrogates this initial volume loss in neutrophil-like differentiated HL-60 (dHL-60) cells ( fig. S1F ). (elifesciences.org)
  • In our study, we have investigated the potential role of OAG in Ca(2)(+) influx-mediated NOX2 activity in neutrophil-like-differentiated HL-60 cells to further characterize second signals involved in the regulation of NOX2. (uni.lu)
  • We used VES and FBZ, at low concentrations, singly and in combination, to test their inhibitory effects on proliferation of human and mouse prostate cancer cells in vitro. (researchgate.net)
  • Administered alone, FBZ inhibited proliferation faster than VES in both mouse and human prostate cancer cell lines and a synergistic effect between both was also observed. (researchgate.net)
  • Most cancer cells undergo metabolic reprogramming that support their survival and proliferation. (degruyter.com)
  • Downregulation of KLF8 inhibits proliferation and glycolysis, and also promotes apoptosis in AML cells via AKT/mTOR pathway. (ajol.info)
  • In previous research, the authors demonstrated that the methanol extract of Artemisia vulgaris (AVM) has the ability to inhibit chronic myeloid leukemia (CML) cell proliferation. (spandidos-publications.com)
  • Extracts prepared from Trametes versicolor under the trade name of I'm-Yunity exhibit anti-tumorigenic activities, as supported by inhibition of the proliferation and induction of apoptosis in malignant cells. (spandidos-publications.com)
  • The broad spectrum anti-tumor effects of calcitriol and analogs are mostly based on inhibition of cancer cell proliferation and invasiveness, induction of differentiation and apoptosis, and promotion of angiogenesis. (jcancer.org)
  • Morphine has been shown to negatively impact macrophage function by impairing the proliferation of macrophage progenitor cells, inhibiting cytokine secretion leading to decreased chemotaxis, hindering the ability of macrophages to directly phagocytize pathogens, and inhibiting nitric oxide production. (uspharmacist.com)
  • C3G decreased the proportion of CD38+ cells, RASF proliferation, and proinflammatory cytokine secretion, and it increased the Treg cell proportion. (biomedcentral.com)
  • The inhibitory effects of bufalin on cell proliferation were determined by MTT (Methyl thiazolyltetrazolium) assay. (biomedcentral.com)
  • 1) Bufalin inhibited the proliferation of SW620 cells. (biomedcentral.com)
  • Inhibits human HL-60 leukemia cell differentiation induced by Am80 (IC50 = 150 nM). (fishersci.com)
  • Calcitriol elicits anti-tumor effects mainly through the induction of cancer cell apoptosis, cell cycle arrest, differentiation, angiogenesis and the inhibition of cell invasiveness by a number of mechanisms. (jcancer.org)
  • MAP kinase-dependent autophagy controls phorbol myristate acetate-induced macrophage differentiation of HL-60 leukemia cells. (ac.rs)
  • We investigated the mechanisms and the role of autophagy in the differentiation of HL-60 human acute myeloid leukemia cells induced by protein kinase C (PKC) activator phorbol myristate acetate (PMA). (ac.rs)
  • PMA-triggered differentiation of HL-60 cells into macrophage-like cells was confirmed by cell-cycle arrest accompanied by elevated expression of macrophage markers CD11b, CD13, CD14, CD45, EGR1, CSF1R, and IL-8. (ac.rs)
  • Pharmacological or genetic inhibition of ERK or JNK suppressed PMA-triggered nuclear translocation of TFEB and FOXO1/3, ATG expression, dissociation of pro-autophagic beclin-1 from its inhibitor BCL2, autophagy induction, and differentiation of HL-60 cells into macrophage-like cells. (ac.rs)
  • Pharmacological or genetic inhibition of autophagy also blocked PMA-induced macrophage differentiation of HL-60 cells. (ac.rs)
  • Therefore, MAP kinases ERK and JNK control PMA-induced macrophage differentiation of HL-60 leukemia cells through AMPK/mTORC1-independent, TFEB/FOXO-mediated transcriptional and beclin-1-dependent post-translational activation of autophagy. (ac.rs)
  • Mandic M, Misirkic Marjanovic M, Vucicevic L, Jovanovic M, Bosnjak M, Perovic V, Ristic B, Ciric D, Harhaji-Trajkovic L, Trajkovic V. MAP kinase-dependent autophagy controls phorbol myristate acetate-induced macrophage differentiation of HL-60 leukemia cells. (ac.rs)
  • This study also demonstrates that the inhibition of Treg cell differentiation in MNCs by CD38+ NK cells is a potential cause of the immune imbalance in RA and CIA. (biomedcentral.com)
  • During the differentiation process, the cells were treated with the program chain "pathogene Ai" of the BICOM® optima mobile bioresonance device on three consecutive days in an external mini-incubator at 37 °C for 180 min each. (bioresonance.org)
  • Stem and progenitor cell populations are often heterogeneous, which may reflect stem cell subsets that express subtly different properties, including different propensities for lineage selection upon differentiation, yet remain able to interconvert. (lu.se)
  • A key challenge is to understand how state, but must also afford flexibility in cell-fate choice to permit the different cell-fate options confronting stem and progenitor cell-type diversification and differentiation in response to cells are selected and coordinated such that adoption of a given intrinsic cues or extrinsic signals. (lu.se)
  • Evidence the fate of stem cells has broad ramifications for biomedical suggests that during development or differentiation, cells make science from elucidating the causes of cancer to the use of very precise transitions between apparently stable ``network stem cells in regenerative medicine. (lu.se)
  • 8-10 It inhibits nitric oxide production 11 and cyclooxygenases 1 and 2, 9 and induces apoptosis in HL-60 leukemia cells via caspase 3 activation. (ascopost.com)
  • In HL-60 leukaemia cells, di-GA activated caspase 3 and dose-dependently induced apoptosis. (nature.com)
  • KYC dose-dependently inhibited MPO-NaSCN-mediated LDL oxidation (Fig. 8C). (technumber.com)
  • The researchers also determined the IC50 of P. alata (ELPA) leaf extracts against both cancer (HCT-116, SF-295, OVACAR-8 and HL-60) cells and non-cancer cells (PBMC). (naturalnews.com)
  • IC50 or half maximal inhibitory concentration is a measure of the effectiveness of a substance in inhibiting a biochemical function. (naturalnews.com)
  • Among the Passiflora leaf extracts evaluated, ELPA exhibited the highest cytotoxicity against HL-60 cells (IC50 19.37 u g/mL) but was not cytotoxic against PBMC, suggesting selectivity for cancer cells. (naturalnews.com)
  • AVM induced the release of cleaved PARP and cleaved caspase‑3 caused apoptosis and inhibited the viability of these cells. (spandidos-publications.com)
  • By assaying their anti-proliferative and anti-apoptotic effects using human promyelocytic HL-60 cells, we found that the ethanolic extract of I'm-Yunity-Too was more active in inducing cell death compared to I'm-Yunity, based on measured changes in the expression of caspase 3 and Bax. (spandidos-publications.com)
  • These chemicals are characterized by their low molecular weight and their ability to inhibit the progress of certain infections. (hindawi.com)
  • In previous research, the authors demonstrated that the methanol extract of A. vulgaris (AVM) has the ability to inhibit the viability of CML cells ( 10 ). (spandidos-publications.com)
  • BocPLPLP inhibited leukotriene B4-induced rises in [Ca2+]i with a Ki of 0.33 microM, whereas CsH showed no inhibitory effect. (rspharmchem.com)
  • exerted the survival or growth inhibitory effect on HL-60 human leukemia cells as well as antimutagenic activity, and the dichloromethane fr. (jcpjournal.org)
  • It further inhibited cell-cycle progression in the G1 phase by four different mechanisms: rapid downregulation of cyclin D1, induction of Chk2 with simultaneous downregulation of Cdc25A, induction of the Cdk-inhibitor p21 Cip/Waf and inhibition of ribonucleotide reductase activity resulting in reduced dCTP and dTTP levels. (nature.com)
  • Through NHE1 inhibition in primary human neutrophils, we show that cell swelling is both necessary and sufficient for rapid migration following chemoattractant stimulation. (elifesciences.org)
  • CsH inhibited FMLP binding in HL-60 membranes with a Ki (inhibition constant) of 0.10 microM. (rspharmchem.com)
  • Gyp-L-induced cell death was associated with lysosomal swelling and autophagic flux inhibition. (oncotarget.com)
  • The kimchi fractions inhibited the survival or growth of HL-60 human leukemia cells in the sulforhodamine B(SRB) assay, 3-(4,5-dimethylthiazol)-2, 5-diphenyltetrazolium bromide(MTT) assay and growth inhibition test. (jcpjournal.org)
  • In a study published in the Journal of Medicinal Plants Research , Brazilian researchers investigated the cytotoxicity of various Passiflora species using colon cancer, ovarian cancer and leukemia cell lines. (naturalnews.com)
  • These compounds proved to be highly cytotoxic to the leukemia cell line HL-60 but not to normal cancer cells, suggesting a selectivity to cancer cells that could prove beneficial in clinical settings. (naturalnews.com)
  • This pathway should be particularly important during inflammation whereby oxidative/nitrosative burst generated by macrophages may cause direct nitrosylation or trans-nitrosylation of APT in target cells. (cdc.gov)
  • RAW 264.7 macrophages elicited enhanced phagocytizing activity towards "nitrosylated" HL-60 cells. (cdc.gov)
  • MiRNAs appear to target about 60% of the genes of humans and other mammals. (wikipedia.org)
  • Our scientists pursue every aspect of cancer research-from exploring the biology of genes and cells, to developing immune-based treatments, uncovering the causes of metastasis, and more. (mskcc.org)
  • PMA also increased nuclear translocation of autophagy transcription factors TFEB, FOXO1, and FOXO3, as well as the expression of several autophagy-related (ATG) genes in HL-60 cells. (ac.rs)
  • explosion further, consider that a fictitious small genome with 2002) More recently and more dramatically, the potential for 260 genes would host the same number of combinations as cell state conversions is exemplified by the reprogramming of the number of atoms in the visible universe! (lu.se)
  • Thus, the secretion of gall wasps stimulates plant cell growth and overrules homeostasis of the affected leaf area - this is similar to tumour outgrowth. (nature.com)
  • C3G also elevated Sirt6 expression to suppress NKG2D expression, increase TNF-α secretion, and decrease IFN-γ secretion in CD38+ NK cells, which stimulates MNCs to differentiate into Treg cells. (biomedcentral.com)
  • To varying degrees, these fates also extend to the Such state stability is required in stem and progenitor cells to immediate progeny of stem cells, known as progenitor or support self-renewal and maintenance of the uncommitted transit-amplifying cells. (lu.se)
  • Despite high ORAC values, neither purified juglone nor the extract showed protective effects on HL-60 cells under oxidative conditions. (ejbiotechnology.info)
  • Based on these results, the researchers concluded that the cytotoxic activity of ELPA against cancer cells may be due to the synergistic action of flavonoids in it, which induces cell death by apoptosis and necrosis. (naturalnews.com)
  • They then evaluated the in vitro cytotoxicity of these extracts against cancer cell lines using MTT assay. (naturalnews.com)
  • Most of these compounds inhibited the in vitro growth of B16 melanoma cells. (noniresearch.org)
  • This study evaluated the in vitro effects of the combination of PKC412 and ara-C or daunorubicin, studying the effect of co-incubation, pre-incubation and sequential incubation of the drugs in patient samples and cell lines. (lu.se)
  • Inflammation is an active defense reaction against diverse insults, designed to remove or inactivate noxious agents and to inhibit their detrimental effects. (420magazine.com)
  • During inflammation, binding of selectins to cell-surface glycoconjugates mediates rolling adhesion of leukocytes on vascular surfaces. (ashpublications.org)
  • When CIA rats were injected with both C3G and the Sirt6 inhibitor OSS_128167, the rats exhibited joint inflammation and a low Treg cell proportion, but the CD38+ NK proportion was still low. (biomedcentral.com)
  • They play an essential part of the innate immune system and as inflammation-mediating cells. (bioresonance.org)
  • They play a two fold role as (i) phagocytes (= scavenger cells) by floating in the circulating blood and forming a cellular defense against invading microbial pathogens as an essential part of the innate immune system [1], and (ii) as inflammation-mediating cells after having entered the inflamed tissue. (bioresonance.org)
  • All the kimchi fractions inhibited survival of HL-60 leukemia cells more than 50% in the addition concentration of 0.2 mg/assay, in particular dichloromethane fr. (jcpjournal.org)
  • It is thus concluded that AVM inhibits the activity of BCR/ABL and their subsequent molecular signals, including AKT and MAPK, resulting in cytotoxicity via apoptosis. (spandidos-publications.com)
  • Cells extend their membranes during morphogenesis and movement. (elifesciences.org)
  • CsH inhibited activation by FMLP of high affinity GTPase (the enzymatic activity of alpha-subunits of heterotrimeric regulatory guanine nucleotide-binding proteins) in HL-60 membranes with a Ki of 0.79 microM. (rspharmchem.com)
  • We explored whether LPL affects (a) lipoprotein transport across bovine aortic endothelial cells or (b) lipoprotein binding to subendothelial cell matrix (retention). (jci.org)
  • 1 , 2 P- and E-selectin, expressed on activated platelets and/or endothelial cells, bind to ligands on leukocytes. (ashpublications.org)
  • L-selectin, expressed on leukocytes, binds to ligands on some endothelial cells and on other leukocytes. (ashpublications.org)
  • These data suggest KYC is certainly a book and particular inhibitor of MPO activity that's non-toxic to 1353859-00-3 supplier endothelial cell civilizations. (technumber.com)
  • The protein-based communication between leukocytes and inflamed endothelial cells leading to diapedesis has been largely investigated and several key players such as IL6, TNFα, or the damage associated molecular pattern molecule (DAMP) calprotectin are now well identified. (uni.lu)
  • Therefore, we examined the inflammatory impact of IL27 on primary endothelial cells and the potentially modulatory effect of calprotectin on both transcriptome and proteome levels. (uni.lu)
  • Endothelial cells are the primary site of plasma TFPI synthesis. (medscape.com)
  • 4] Most circulating TFPI is bound to lipoproteins, but on the surface of endothelial cells, a second pool of TFPI is bound to heparan sulfates. (medscape.com)
  • When heparin is administered, the endothelial cell-bound TFPI is released and the plasma level rises severalfold. (medscape.com)
  • This review discusses the anti-inflammatory properties of cannabinoid compounds at molecular, cellular and whole animal levels, first by examining the evidence for anti-inflammatory effects of cannabinoids obtained using in vivo animal models of clinical neuroinflammatory conditions, specifically rodent models of multiple sclerosis, and second by describing the endogenous cannabinoid (endocannabinoid) system components in immune cells. (420magazine.com)
  • The plaques contain lipids, inflammatory cells, smooth muscle. (msdmanuals.com)
  • High abundance of glucose in the cytoplasm of a cancer cell also increases flux into other metabolic pathways such as hexosamine biosynthetic pathway (HBP) [ 7 ]. (degruyter.com)
  • The purpose of the research is to investigate the roles of Jak-STAT3 signaling pathway in bufalin-induced apoptosis in colon cancer SW620 cells. (biomedcentral.com)
  • The chronic effect of PMA was inhibited by 10 nM actinomycin D or 7 microM cycloheximide. (jci.org)
  • CsH inhibited the stimulatory effects of FMLP on cytosolic Ca2+ concentration ([Ca2+]i), O2- formation, and beta-glucuronidase release with Ki values of 0.08, 0.24, and 0.45 microM, respectively. (rspharmchem.com)
  • This polyphenolic compound is known to be a strong anti-oxidant which scavenges reactive oxygen species (ROS) directly or effectively inhibits ROS-generating enzymes ( e.g. xanthine oxidase, lipoxygenase). (rsc.org)
  • Mechanistic investigations revealed that through increasing the levels of intracellular reactive oxygen species (ROS), Gyp-L triggered protein ubiquitination and endoplasm reticulum (ER) stress response, leading to Ca 2+ release from ER inositol trisphosphate receptor (IP 3 R)-operated stores and finally cell death. (oncotarget.com)
  • The cell survival rate was measured using the principle of water-soluble tetrazolium salt-1 assay, which is a measurement method for cell survival rate. (researchgate.net)
  • Senescence-associated β-galactosidase (SA-β-gal) assay was implemented to dye β-galactosidases (used as a cell aging biomarker) to measure HDF cell aging by UVA. (researchgate.net)
  • HL-60 cells externalized PS in response to SNCEE or GSNO treatment as evidenced by annexin V binding assay and fluorescence microscopy. (cdc.gov)
  • This indicates the intricate control of metabolism and redox balance for survival of cancer cells that can be targeted by therapeutic agents. (degruyter.com)
  • Neurotrophic factors, including brain-derived neurotrophic factor (BDNF), are indispensable regulators for the survival of glial cells and neurons in the central nervous system (CNS) [ 13 ]. (degruyter.com)
  • Opinions are embraced and defended, but transfusion of red blood cells has not reliably demonstrated increased survival, other than in 2 specific populations, as follows: (1) those with active hemorrhage, and (2) those with active cardiac ischemia. (medscape.com)
  • We speculate that macrophage induced nitrosative stress contributes to effective clearance of apoptotic cells. (cdc.gov)
  • Drugs used in combination chemotherapy, such as oxaliplatin, leucovorin calcium, and fluorouracil, work in different ways to stop the growth of tumor cells, either by killing the cells, by stopping them from dividing, or by stopping them from spreading. (stanford.edu)
  • Immunotherapy with monoclonal antibodies, such as atezolizumab, may help the body's immune system attack the cancer, and may interfere with the ability of tumor cells to grow and spread. (stanford.edu)
  • Some block the ability of tumor cells to grow and spread. (stanford.edu)
  • Others find tumor cells and help kill them or carry tumor-killing substances to them. (stanford.edu)
  • Quercetin -rich vegetables such as onion and lettuce in meals are quite popular, and the daily intake of quercetin in Western countries is estimated to be 10-20 mg. 3 The extensive studies using cell cultures and experimental animals have clearly indicated its anti-atherosclerotic and anti-carcinogenic effects, as well as its protective action in the central nervous system (CNS). (rsc.org)
  • The activities of these compounds against two human cancer cell lines (HL-60 and AZ521), and their inhibitory effects on Epstein-Barr virus early antigen (EBV-EA) activation, were also evaluated. (noniresearch.org)
  • We studied the effects of CsH in comparison with those of N-t- butoxycarbonyl-L-phenylalanyl-L-leucyl-L-phenylalanyl-L-leucyl-L- phenylalanine (BocPLPLP), a well known formyl peptide receptor antagonist, and of other Cs on activation of N6,2'-O-dibutyryl adenosine 3:5'-monophosphate-differentiated HL-60 cells and human erythroleukemia cells (HEL cells). (rspharmchem.com)
  • Opioids have considerable effects on cellular function across many immune cell lineages, affecting both the innate and adaptive immune systems. (uspharmacist.com)
  • In cell lines, antagonistic effects were seen frequently in HL-60 (90%) and less so in MV4-11 (60%) regardless of sequence or timing of the drugs. (lu.se)
  • The combination of daunorubicin and PKC412 resulted in more synergistic and less antagonistic effects compared to combinations with ara-C, in both patient material and cell lines. (lu.se)
  • The combination of Lonafarnib, a farnesyl-transferase inhibitor (FTI) and PKC412 had additive and synergistic effects in both FLT3/ITD positive and negative cell lines. (lu.se)
  • In turn, the plant produces GA, which seems to combat the improper growth signals and re-establishes cell-cycle control. (nature.com)
  • The growth and apoptosis of AML cells were determined using CCK-8, 5-ethynyl-2-deoxyuridine (EdU), flow cytometry, and western blot assays. (ajol.info)
  • Bufalin not only inhibited the growth of colon cancer SW620 cells, but also induced apoptosis of SW620 cells. (biomedcentral.com)
  • PKC412 inhibits the growth of leukemic cell lines with FLT3 mutations such as the MV4-11. (lu.se)
  • Redirection of glutamine to cellular energetics destabilizes glutathione homeostasis in glioblastoma cells and other cancer cells [ 9 ]. (degruyter.com)
  • specifically, evidence showing that cannabinoids inhibit neuroinflammation and that immune cells express the entire machinery that constitutes a functional cannabinoid signaling system. (420magazine.com)
  • 1993), expressed primarily by immune cells. (420magazine.com)
  • 2 Previous studies have evaluated the opioid receptors on a wide array of immune cells. (uspharmacist.com)
  • Cancer cells often maintain high basal level of ROS and are vulnerable to any further increase in ROS levels beyond a certain protective threshold. (degruyter.com)
  • Protein activation and expression were analysed by western blotting, deoxyribonucleoside triphosphate levels by HPLC, ribonucleotide reductase activity by 14 C-cytidine incorporation into nascent DNA and cell-cycle distribution by FACS. (nature.com)
  • This complementarity was proposed to inhibit the translation of the lin-14 mRNA into the LIN-14 protein. (wikipedia.org)
  • Long-term activation of protein kinase c causes chronic Na/H antiporter stimulation in cultured proximal tubule cells. (jci.org)
  • To examine the role of protein kinase C as a chronic regulator of proximal tubule Na/H antiporter activity, the effect of phorbol 12-myristate 13-acetate (PMA) on the Na/H antiporter was studied in cultured proximal tubule cells. (jci.org)
  • This latter effect was due to protein kinase C activation in that it was inhibited by sphingosine and was not seen with 4 alpha-PMA, an inactive analogue. (jci.org)
  • Apoptosis increased in KG-1 and HL-60 cells, with enhanced Bax and reduced Bcl-2 protein levels, after transfection with sh-KLF8. (ajol.info)
  • Interestingly, there existed a reciprocal positive-regulatory loop between Ca 2+ release and ER stress in response to Gyp-L. In addition, protein synthesis was critical for Gyp-L-mediated ER stress and cell death. (oncotarget.com)
  • 13 Morphine has been shown to decrease IL-23 production in murine models, decreasing dendritic cell antimicrobial protein production. (uspharmacist.com)
  • PMA failed to activate autophagy inducer AMP-activated protein kinase (AMPK) and inhibit autophagy suppressor mechanistic target of rapamycin complex 1 (mTORC1). (ac.rs)
  • OAG inhibited fMLF- and thapsigargin-induced Ca(2)(+) entry, a phenomenon that was not restored by protein kinase C (PKC) or PI3K blockade. (uni.lu)
  • Green husk extract generates an antiproliferative effect in HL-60 cells, which is related to an induction of the early stages of apoptosis and a loss of mitochondrial membrane potential. (ejbiotechnology.info)
  • Of note, an increased carbohydrate uptake and/or induction of death receptors of cancer cells was exploited to develop glycoconjugates that potentially induce cellular stress, ROS and apoptosis. (degruyter.com)
  • Taken together, this work suggested a novel therapeutic option by Gyp-L through the induction of an unconventional ROS-ER-Ca 2+ -mediated cell death in human esophageal cancer. (oncotarget.com)
  • In terminally differentiated cell fate is coupled to appropriate regulation of the alternative cells, transcriptional networks must be stable and irreversible, pathways. (lu.se)
  • Due to the increased drug and radiation tolerance, it is urgent to develop novel anticancer agent that triggers nonapoptotic cell death to compensate for apoptosis resistance. (oncotarget.com)
  • This mini review highlights the development of compounds that target cancer cells by taking advantage of redox or metabolic alteration in cancer cells. (degruyter.com)
  • Additionally, juglone is present in the green husk of walnut, which suggests evaluating the effect of GH extracts on carcinogenic cell lines. (ejbiotechnology.info)
  • prostate cancer cell lines and a synergistic effect between both was also o bserved. (researchgate.net)
  • Laboratory studies suggest that ginger can protect brain cells from the plaques that cause Alzheimer's disease, but this effect has not been studied in humans. (mskcc.org)
  • This study investigated the pathogenic role of CD38+ NK cells and the effect of C3G on RA. (biomedcentral.com)
  • In the patient cells PKC412 exerted its effect at concentrations between 0.1 and 2.0 muM. (lu.se)
  • The results revealed that AVM inhibited the phosphorylation of BCR/ABL and their subsequent molecular signals including AKT and MAPK activation. (spandidos-publications.com)
  • It is well known that the cells of our body emit and receive electromagnetic signals which allow an intra- and intercellular electromagnetic communication [13,14]. (bioresonance.org)
  • The cell- ations in these signals (see Table 1 for a glossary of terms). (lu.se)
  • Therefore, we hypothesized that nitrosative stress - acting through APT Snitrosylation - enhances PS externalization in cells by inhibiting APT activity. (cdc.gov)
  • Chemoattractant exposure increases cell volume and potentiates neutrophil migration, but the causal link between these processes is not known. (elifesciences.org)
  • Our data demonstrate that cell swelling complements cytoskeletal inputs for chemoattractant-induced potentiation of migration. (elifesciences.org)
  • This paper will be of interest to cell biologists and biophysicists working on cell migration. (elifesciences.org)
  • In contrast to the extensive work characterizing the actin regulators that underlie cell motility, we have a relatively poor understanding of the molecular regulators of water influx during cell migration. (elifesciences.org)
  • Neutrophils are a powerful system to study the biophysical demands of cell motility, as they acutely initiate rapid migration following stimulation with chemoattractant. (elifesciences.org)
  • In 1994, through application of broad range molecular cell signal transduction and possibly eukaryotic transcrip- amplification and DNA sequencing, the causative agent tion. (cdc.gov)
  • Correspondence with low molecular weight chemicals that results in the priming of chemical-specific, Prof. Dr. Stefan F. Martin, Allergy Research skin-homing CD8+ Tc1/Tc17 and CD4+ Th1/Th17 cells. (cdc.gov)
  • BocPLPLP was 14-fold less potent than CsH in inhibiting FMLP binding and 4- to 6-fold less potent than CsH in inhibiting FMLP-induced GTP hydrolysis, rises in [Ca2+]i, O2- formation, and beta-glucuronidase release. (rspharmchem.com)
  • CsH and BocPLPLP did not inhibit the rises in [Ca2+]i induced by several other stimuli in HL-60 cells and HEL cells. (rspharmchem.com)
  • While the involvement of actin polymerization in membrane protrusion is well-established, we have a more limited understanding of the role of transmembrane water flow in cell motility. (elifesciences.org)
  • The normal cells were not affected when juglone is present at concentrations of 1 μM, while at higher concentrations, there is loss of viability of both cancerous and healthy cells. (ejbiotechnology.info)
  • It is likely that cytoskeletal rearrangements and water influx collaborate in a wider range of cell morphological and migratory contexts than are currently appreciated. (elifesciences.org)
  • The morphological changes of cells were measured by Wright-Giemsa staining. (biomedcentral.com)
  • Interestingly, AVM appeared to be more sensitive to imatinib‑resistant (T315I, Y253H, and E279K) than wild‑type BCR/ABL cells, indicating its potential to overcome imatinib‑resistant severe issues in CML. (spandidos-publications.com)
  • To experimentally address this hypothesis we utilized HL-60 cells that express high activity of APT. (cdc.gov)
  • KYC particularly inhibits the experience of MPO that's released from PMA-activated HL-60 cells however, not NOX activity that's needed for HL-60 cells to create O2? (technumber.com)
  • Treatment with the BICOM® mobile bioresonance device had no significant influence on mitotic activity or cell size of functional neutrophils. (bioresonance.org)
  • The transcriptional and translational levels of KLF8 in AML cell lines were determined by quantitative real time-polymerase chain reaction (qRT-PCR) and western blotting. (ajol.info)
  • Similarly, GATA-1 has been shown to induce lineage switching expression values even if, for simplicity, we assume only ``on'' of committed cells in hematopoiesis, first in cell lines (Kulessa and ``off'' states for each gene. (lu.se)
  • BACKGROUND: We have recently reported a novel mutation in the beta3 subunit of the platelet fibrinogen receptor (alpha(IIb)beta3D723H) identified in a patient with dominantly inherited macrothrombocytopenia, and we have shown that this mutation promotes a new phenotype in Chinese hamster ovary (CHO) cells, characterized by fibrinogen-dependent, microtubule-driven proplatelet-like cell extensions. (uni.lu)
  • Furthermore, di-GA inhibited the generation of lymphendothelial gaps by cancer cell spheroid-secreted lipoxygenase metabolites. (nature.com)
  • a single cell volume measurement technique, to primary human neutrophils. (elifesciences.org)
  • We observed that P- and E-selectin bound to pronase-sensitive ligands on murine monocytic WEHI-3 cells and murine neutrophils, indicating that the ligands for both selectins are glycoproteins. (ashpublications.org)
  • CSLEX-1, HECA-452, and other widely used mAbs to sLe x and Le x did not bind to WEHI-3 cells and bound at very low levels to murine neutrophils. (ashpublications.org)
  • Compared to human promyelocytic HL-60 cells, WEHI-3 cells and murine neutrophils expressed low α1-3-fucosyltransferase activities. (ashpublications.org)
  • Consistent with very low endogenous fucosylation, forced fucosylation of intact WEHI-3 cells or murine neutrophils by exogenous α1-3-fucosyltransferase FTVI and GDP-fucose created many new epitopes for anti-sLe x mAbs such as HECA-452 and CSLEX-1. (ashpublications.org)
  • Neutrophils are the most common type of granulocyte, a type of white blood cell, found in most mammals. (bioresonance.org)
  • Human promyelocytes (HL-60 cells) were differentiated to functional neutrophils over a period of 5 days by the addition of 1.5 vol% dimethyl sulfoxide to the culture medium. (bioresonance.org)
  • Finally, the basal cell metabolism and the generation of superoxide anion radicals with and without treatment of the functional neutrophils was examined. (bioresonance.org)
  • After 60 min, the animals were treated with saline, carbamazepine and hydroalcoholic plant extracts. (bvsalud.org)
  • 11,12 Dendritic cells are essential to the innate immune system through their presentation of foreign antigens to T-cells. (uspharmacist.com)
  • C3G also significantly increased the level of interleukin (IL)-10 and the regulatory T (Treg) cell proportion, and it decreased the interleukin (IL)-6 and interferon (IFN)-γ levels and CD38+ NK cell proportion in rat peripheral blood and synovial fluid. (biomedcentral.com)
  • Furthermore, C3G stimulated MNCs to increase IL-2 and IL-10 production and the Treg cell proportion, and it caused MNCs to decrease IL-6 and IFN-γ production and the CD38+ NK cell proportion. (biomedcentral.com)
  • Although CD38+ NK cells significantly decreased the Treg cell proportion and IL-10 level in MNCs, CD38+ NK cells that had been pretreated with C3G increased the proportion of Treg cells and IL-10 levels and decreased the IL-6 and IFN-γ levels in the coculture. (biomedcentral.com)
  • When MNCs were cocultured with C3G-pretreated CD38+ NK cells in the presence of TNF-α and an anti-IFN-γ antibody, the IL-10+ Treg cell proportion significantly increased. (biomedcentral.com)
  • Treg, regulatory T cell. (cdc.gov)