• In addition, the lab is actively engaged in studies examining regulation of contractility by microtubules and other cytoskeletal elements, cardiotoxicity associated with VEGF-anti-angiogenic tyrosine kinase inhibitors, disease-associated shifts in cardiac metabolism, mechanisms of inherited cardiomyopathies, advanced myocardial phenotyping using digital pathology and machine learning techniques, and integrated genomic inquiries designed to identify molecular mechanisms of myocardial pathology. (upenn.edu)
  • The mechanism by which the kinase inhibitors led to CGG repeat reduction involved triggering DNA damage repair pathways. (biocentury.com)
  • Finding physiologically relevant, reproducible, and reliable cell models that can be used to detect these end points at preclinical and discovery stages has been a challenge, as most toxicity testing is done either by using immortalized cancer cell lines, primary explanted somatic cells, or live animals. (the-scientist.com)
  • The iPSC technology was pioneered by Shinya Yamanaka and Kazutoshi Takahashi in Kyoto, Japan, who together showed in 2006 that the introduction of four specific genes (named Myc, Oct3/4, Sox2 and Klf4), collectively known as Yamanaka factors, encoding transcription factors could convert somatic cells into pluripotent stem cells. (wikipedia.org)
  • Human induced pluripotent stem (iPS) cells can differentiate into somatic cells ( 1 ). (spandidos-publications.com)
  • The compound can also be used in protocols for the chemical reprogramming of somatic cells to iPSCs. (tocris.com)
  • Induced pluripotent stem cells (also known as iPS cells or iPSCs) are a type of pluripotent stem cell that can be generated directly from a somatic cell. (wikipedia.org)
  • citation needed] Since iPSCs can be derived directly from adult tissues, they not only bypass the need for embryos, but can be made in a patient-matched manner, which means that each individual could have their own pluripotent stem cell line. (wikipedia.org)
  • dubious - discuss] In his Nobel seminar, Yamanaka cited the earlier seminal work of Harold Weintraub on the role of myoblast determination protein 1 (MyoD) in reprogramming cell fate to a muscle lineage as an important precursor to the discovery of iPSCs. (wikipedia.org)
  • iPSCs are typically derived by introducing products of specific sets of pluripotency-associated genes, or "reprogramming factors", into a given cell type. (wikipedia.org)
  • Unlike the first generation of iPSCs, these second generation iPSCs produced viable chimeric mice and contributed to the mouse germline, thereby achieving the 'gold standard' for pluripotent stem cells. (wikipedia.org)
  • Here we show the differentiation of patient specific iPSCs derived from a patient with SFTPB deficiency into lung organoids with mesenchymal and epithelial cell populations from both the proximal and distal portions of the human lung. (nature.com)
  • Infection of iPSCs with lentiviral inserts is a highly efficient process since stem cells grow quickly, remain undifferentiated in specific cell culture conditions and can establish fully infected clones within 2-3 passages. (nature.com)
  • Using induced pluripotent stem cells (iPSCs), we generated mature multiciliated cells in a functional airway epithelium. (ca.gov)
  • Our report demonstrating the generation of mature multiciliated cells in respiratory epithelium from iPSCs is a significant advance toward modeling a number of human respiratory diseases in vitro. (ca.gov)
  • We will use methodology described by Cader to derive nociceptors from human induced pluripotent stem cells (iPSCs) [4]. (ox.ac.uk)
  • Derivation of nociceptors from human induced pluripotent stem cells (iPSCs) with validation of activation assays using recombinant NGF, angiotensin and human OA synovial fluid. (ox.ac.uk)
  • Induced pluripotent stem cells, iPSCs, are mature, differentiated cells, such as skin cells, that are reprogrammed in the laboratory to be similar to undifferentiated embryonic stem cells. (jove.com)
  • To create iPSCs, mature cells, like skin fibroblasts, are taken from a patient and put into culture. (jove.com)
  • These cells are also pluripotent like embryonic stem cells-able to produce all cell types-and are therefore called induced pluripotent stem cells (iPSCs). (jove.com)
  • iPSCs are potentially valuable in medicine, because a patient who needs a particular cell type-for instance, someone with a damaged retina due to macular degeneration-could receive a transplant of the required cells, generated from another cell type in their own body. (jove.com)
  • To create iPSCs, mature cells such as skin fibroblasts or blood cells from a person are grown in culture. (jove.com)
  • It is still being studied whether iPSCs are truly equivalent to embryonic stem cells, but they appear to be similar and can produce cells from all three germ layers of the body. (jove.com)
  • As with other types of stem cells, scientists are learning how to promote the differentiation of specific cell types from iPSCs efficiently, so that the needed cell types can be produced in adequate quantities. (jove.com)
  • Here, we assessed the potential therapeutic effect of HDAC6 inhibitors on peripheral neuropathy with HSPB1 mutation using in vitro model of motor neurons derived from induced pluripotent stem cells (iPSCs) of CMT2F and dHMN2B patients. (ewha.ac.kr)
  • Our results suggest that the neurons derived from patient-specific iPSCs can be used in drug screening including HDAC6 inhibitors targeting peripheral neuropathy. (ewha.ac.kr)
  • Histone acetylation has been implicated to be important for the pluripotent cell state, as some HDAC inhibitors have been shown to improve reprogramming efficiency of induced pluripotent stem cells (iPSCs) or maintain a pluripotent stem cell state. (biomedcentral.com)
  • Maintaining DNA stability in induced pluripotent stem cells (iPSCs) and iPSCs-derived neurons is a challenge in their clinical application. (bvsalud.org)
  • UV treatment induced a significantly decreasing in the cell viability of iPSCs-derived neurons by ~76.1%, whereas ~20.8% in primary retinal neurons . (bvsalud.org)
  • Moreover, using the Ku80 inhibitor Compound L, our data showed that Ku80 promotes the DNA stability and cell viability of iPSCs-derived neurons . (bvsalud.org)
  • Here, we describe an approach that allows splenic B cells activated to undergo Ig heavy-chain (IgH) class-switch recombination (CSR) to be reprogrammed into iPSCs that contribute to high-grade chimerism in mice. (northwestern.edu)
  • We find that anti-CD40 plus IL-4-activated B cells produce iPSCs that are uniformly hypermethylated in the imprinted Dlk1-Dio3 gene cluster and fail to produce chimerism in mice. (northwestern.edu)
  • However, treatment of activated B cells with the methyltransferase inhibitor 5-aza-2′-deoxycytidine before and at early stages of reprogramming attenuates hypermethylation of the Dlk1-Dio3 locus in resultant iPSCs and enables them to form high-grade chimerism in mice. (northwestern.edu)
  • These conditions allowed us to produce chimeric mice in which all mature B cells were derived entirely from IgG1-expressing B-cell-derived iPSCs. (northwestern.edu)
  • We conclude that culture conditions of activated B cells before and at early stages of reprogramming influence the developmental potency of resultant iPSCs. (northwestern.edu)
  • Promotes neurogenesis in human induced pluripotent stem cells (iPSCs) when used in combination with SB 431542 (Cat. (tocris.com)
  • DMH-1 induces intestinal differentiation in human intestinal organoids (hIOs) derived from human pluripotent stems cells (hPSCs). (tocris.com)
  • This strategy provides a useful tool for visualization and identification of neural progeny derived from human pluripotent stem cells. (lu.se)
  • They are 3-dimensional, complex, multicellular structures that can be derived from patient specific human induced pluripotent stem cells (hiPSCs). (nature.com)
  • While human induced pluripotent stem cells (hiPSCs) provide novel prospects for disease-modeling, the high phenotypic variability seen across different lines demands usage of large hiPSC cohorts to decipher the impact of individual genetic variants. (frontiersin.org)
  • Thus, a much higher grade of parallelization, and throughput in the production of hiPSCs is needed, which can only be achieved by implementing automated solutions for cell reprogramming, and hiPSC expansion. (frontiersin.org)
  • We have developed a feeder-free, Sendai virus-mediated reprogramming protocol suitable for cell culture processing via a robotic liquid handling unit that delivers footprint-free hiPSCs within 3 weeks with state-of-the-art efficiencies. (frontiersin.org)
  • Automatically expanded hiPSCs exhibit normal growth characteristics, and show sustained expression of the pluripotency associated stem cell marker TRA-1-60 over at least 5 weeks (10 passages). (frontiersin.org)
  • The advent of the human induced pluripotent stem cells (hiPSCs) technology offers unprecedented opportunities for disease modeling, personalized medicine, and the development of new therapeutic interventions. (frontiersin.org)
  • Although human induced pluripotent stem cells (hiPSCs) are an alternative cell source of ECs and EPCs, the differentiation and purification processes have not been optimized. (nih.gov)
  • Given this, human pluripotent stem cells, such as human embryonic stem cells (hESCs) and induced pluripo- tent stem cells (hiPSCs), have become a unique cell source for study- ing early human brain development. (lu.se)
  • TWS 119 is also offered as part of the Tocriscreen 2.0 Max and Tocriscreen Kinase Inhibitor Library . (tocris.com)
  • DMH-1 is also offered as part of the Tocriscreen 2.0 Max , Tocriscreen Kinase Inhibitor Library and Tocriscreen Stem Cell Library . (tocris.com)
  • Human liver organoids are formed from the assembly of hepatocyte-like cells differentiated from iPS cells, human umbilical vascular endothelial cells and mesenchymal stem cells ( 9 ). (spandidos-publications.com)
  • This study tested the hypothesis that double overexpression of miR-19a & miR-20a (dOex-mIRs) in human induced pluripotent stem cell (iPS)-derived mesenchymal stem cells (MSCs) effectively preserved left-ventricular-ejection-fraction (LVEF) in dilated cardiomyopathy (DCM) (i.e., induced by doxorubicin) rat. (researchsquare.com)
  • We are excited to bring this cutting-edge technology to Lake County, and to partner with academics or companies that are interested in utilizing iPSC-derived endothelial, mesenchymal, or blood cells to advance their own research and product development," said COO Yekaterina Galat. (lakecountypartners.com)
  • Human induced pluripotent stem cell-derived mesenchymal stem cells (iPS-MSCs) are emerging as attractive options for use in cell replacement therapy, but their effect in kidney diseases remains unknown. (oncotarget.com)
  • Successful surgical ways of replacing the diseased endothelium have been clinically tested, and new approaches to accelerate endothelial healing and suppress endothelial-mesenchymal transformation have been proposed including Rho kinase (ROCK) inhibitor eye drops and gene therapy to activate TGF- inhibitor SMAD7. (cancerrealitycheck.com)
  • Many existing protocols for neuronal differentiation of human pluripotent cells result in heterogeneous cell populations and unsynchronized differentiation, necessitating the development of methods for labeling specific cell populations. (lu.se)
  • HDI is based on an HSM with the addition of oncostatin M and an apoptosis inhibitor ( 20 ). (spandidos-publications.com)
  • N-acylated serinol is usually a novel ceramide mimic inducing apoptosis in neuroblastoma cells. (healthweeks.com)
  • In vitro , conditioned medium from iPS-MSCs (iPSMSC-CM) significantly limited albumin-induced tubular apoptosis and enhanced tubular proliferation, accompanied by a reduced expression of tubular Bax and an elevated expression of Bcl2 and survivin. (oncotarget.com)
  • There is a growing body of evidence which demonstrates that following ACS, microRNAs might inhibit fibroblast proliferation and scarring, as well as harmful apoptosis of cardiomyocytes, and stimulate fibroblast reprogramming into induced cardiac progenitor cells. (archivesofmedicalscience.com)
  • Abnormal proliferation, clonal expansion, aberrant differentiation, and diminished apoptosis (programmed cell death) lead to replacement of normal blood elements with malignant cells. (msdmanuals.com)
  • Ion-channel block can lead to acute and delayed arrhythmias, while biochemical toxicities can result in cell death, abnormal cardiac function, and even heart failure and death. (the-scientist.com)
  • She previously worked as a postdoctoral scientist for two years in GlaxoSmithKline's Safety Pharmacology group, where her research focused on developing and validating an electrophysiology assay using human induced pluripotent stem cell-derived cardiomyocytes for cardiac safety screening. (the-scientist.com)
  • Modeling genetic cardiac channelopathies using induced pluripotent stem cells - Status quo from an electrophysiological perspective. (uni-koeln.de)
  • Recognizing shortcomings of 2D formats for inducing cardiac cell maturation or modeling pathological processes, we have increasingly employed a 3D heterocellular model in which cardiac myocytes and fibroblasts self-assemble into contracting cardiac microtissues (CMT) mounted on flexible cantilevers. (upenn.edu)
  • The use of cardiac myocytes derived from human induced pluripotent stem cells (Hu-iPSC-CM), molecular reporters and advances in gene editing provides diverse unprecedented opportunities to rigorously study these fundamental processes in human cardiac cells. (upenn.edu)
  • These inquiries involve utilization of several core capabilities in the Prosser and Margulies laboratories including: super-resolution live-cell microscopy, advanced primary human cardiac myocyte mechanical characterization, and an engineered myocardial microtissue model derived from human induced pluripotent stem cells, and murine models currently being developed. (upenn.edu)
  • Background: Cardiac resynchronization therapy (CRT) induces a significant improvement in patients with heart failure (HF), who are often characterized by the presence of endothelial dysfunction (ED) with impaired flow-mediated vasodilation (FMD). (mirnainhibitor.com)
  • Dr. French and her colleagues have shown a number of hematopoietic pathologies to be successfully recapitulated through differentiation of patient-derived induced pluripotent stem cells, including Glanzmann thrombasthenia, Down syndrome, and juvenile myelomonocytic leukemia. (chop.edu)
  • Basic research utilizing immune-related disease patient-derived induced pluripotent stem (iPS) cells is expected to be a promising platform for elucidating the pathogenesis of the diseases and for drug discovery. (biomedcentral.com)
  • The development of human induced pluripotent stem cell (iPSC)-derived cell lines as models for drug-toxicity testing offers a promising alternative that is more physiologically relevant, more predictive, and more time and cost efficient. (the-scientist.com)
  • Her current research focuses on utilizing cellular impedance measures of human iPSC-derived cardiomyocytes to deconvolute kinase inhibitor cardiotoxicity. (the-scientist.com)
  • Gene therapy has been utilized successfully to repair or inactivate mutations in animal models of monogenic human diseases 19 as well as human in vitro cells 12 . (nature.com)
  • Using a newly developed human neuronal cell model, derived from patient-induced pluripotent stem cells, we find that 2-aminobenzamide histone deacetylase (HDAC) inhibitors increase FXN mRNA levels and frataxin protein in FRDA neuronal cells. (frontiersin.org)
  • Cyclooxygenases Inhibitors Efficiently Induce Cardiomyogenesis in Human Pluripotent Stem Cells. (uni-koeln.de)
  • The Effect of Antiarrhythmic Drugs on the Beat Rate Variability of Human Embryonic and Human Induced Pluripotent Stem Cell Derived Cardiomyocytes. (uni-koeln.de)
  • Human embryonic stem cells also exhibit the Warburg effect ( 15 ). (spandidos-publications.com)
  • and human embryonic stem cells(hESCs). (sigmaaldrich.com)
  • Generation of multiciliated cells in functional airway epithelia from human induced pluripotent stem cells. (ca.gov)
  • Based on the widely cited Essential 8 Medium, Gibco CTS Essential 8 Medium is the first globally available human pluripotent stem cell (hPSC) culture medium with components not directly derived from animals. (thermofisher.com)
  • Human Fibrinogen for Maintenance and Differentiation of Induced Pluripotent Stem Cells in Two Dimensions and Three Dimensions. (mayo.edu)
  • Dr. French came to CHOP in 2008 to establish the Human Pluripotent Stem Cell Core in the Center for Cellular and Molecular Therapeutics. (chop.edu)
  • She is an internationally recognized researcher involved in multi-investigator teams that utilize pluripotent stem cells for modeling human disease to study mechanism, development, and establish new therapeutic modalities. (chop.edu)
  • She is continuing this work using human pluripotent stem cells to study pediatric and adult genetic diseases, including hematological disorders. (chop.edu)
  • The pluripotent stem cell technology represents a major breakthrough for studying disease because these cells express human genes of interest, they can be differentiated in vitro to the three germ layers and derivative tissues, they can be genetically manipulated, and they allow for easy access to cells for in-depth structural, functional, and molecular characterization. (chop.edu)
  • Dr. French is involved in a broad spectrum of projects using these cells to model human development and disease. (chop.edu)
  • In addition to using renewable sources of pluripotent stem cell-derived human hematopoietic lineages for therapeutic and investigative applications, she is genome editing pluripotent stem cells using the CRISPR-CAS9 technology. (chop.edu)
  • Highly Efficient CRISPR-Cas9-Mediated Genome Editing in Human Pluripotent Stem Cells. (chop.edu)
  • Immunocytochemistry and picture analysis Human Ha sido cells had been set with 4% paraformaldehyde (PFA)/PBS or 4% PFA/0.1% glutaraldehyde for 15 min at area temperature or 37°C to conserve tubulin polymers. (healthweeks.com)
  • Similarly, both Ku80 and Ku70 are also expressed in 3D retinal organoids from human embryonic stem cells (ESCs), except for a few Map2-negative cells and the hyaloid vessels of mice E12.5 retinas. (bvsalud.org)
  • Investigating human immune-related diseases using induced pluripotent stem (iPS) cells presents a novel and promising field of study. (biomedcentral.com)
  • We suggest an advantage of utilizing iPS cells for studying human immune-related diseases. (biomedcentral.com)
  • In human disease studies, ethical concerns often restrict the collection of patient cells and tissues. (biomedcentral.com)
  • With iPS cell technology, researchers can readily acquire human cells and investigate these diseases using human-derived samples. (biomedcentral.com)
  • In this way, investigating human immune-related diseases using iPS cells offers distinct advantages over conventional research methods. (biomedcentral.com)
  • In this review, we discuss the application of iPS cells in the study of human immune-related diseases, with an emphasis on autoimmune diseases (Fig. 1 ). (biomedcentral.com)
  • High-Content Assay Multiplexing for Muscle Toxicity Screening in Human-Induced Pluripotent Stem Cell-Derived Skeletal Myoblasts. (tamu.edu)
  • High-Content Assay Multiplexing for Vascular Toxicity Screening in Induced Pluripotent Stem Cell-Derived Endothelial Cells and Human Umbilical Vein Endothelial Cells. (tamu.edu)
  • shRNA-mediated suppression of and genes by adenoviral gene therapy of whole corneal epithelium or only of limbal cells normalized epithelial wound healing altered in human diabetic organ-cultured corneas with restoration of signaling pathways mediated by EGFR-Akt axis (Saghizadeh et al. (cancerrealitycheck.com)
  • Furthermore, downregulation of phosphorylated and/or Fenoterol total EGFR by miR-146a or miR-424 led to a delayed wound healing in cultured telomerase-immortalized human corneal epithelial cells (Funari et al. (cancerrealitycheck.com)
  • We provide detailed protocols for lentiviral transduction, neural differentiation, and subsequent analysis of human embryonic stem cells. (lu.se)
  • However, current protocols for deriving neurons from human pluripotent stem cells give rise to heterogeneous cell populations both in regard to the temporal aspects and the cellular composition. (lu.se)
  • Although possible, these strategies are often complicated to transfer to human cells due to technical issues (9 ), and only a few successful cases have been described ( 10, 11 ). (lu.se)
  • Here we describe how this system can be used for human embry- onic stem cells. (lu.se)
  • In this investigation we sought to answer the question concerning the presence of mast cells in human and rat dental pulp tissues, under normal and inflammatory conditions. (bvsalud.org)
  • Studies using human samples have shown that such cells are present in dental pulps 1,2 whilst others have demonstrated the absence of those cells 3,4,5 . (bvsalud.org)
  • Here, we characterize SpCas9 targeting specificity in human cells to inform the selection of target sites and avoid off-target effects. (cdc.gov)
  • Using the SURVEYOR nuclease assay 13 , we assessed the ability of each Cas9-sgRNA complex to generate indels in human embryonic kidney (HEK) 293FT cells through the induction of DNA doublestranded breaks (DSBs) and subsequent nonhomologous end joining (NHEJ) DNA damage repair (Online Methods). (cdc.gov)
  • Surfactant is produced by alveolar type II cells which can be differentiated in vitro from patient specific induced pluripotent stem cell (iPSC)-derived lung organoids. (nature.com)
  • The influence of melatonin on the heart rhythm - An in vitro simulation with murine embryonic stem cell derived cardiomyocytes. (uni-koeln.de)
  • Pluripotent cells can be differentiated into many different cell types in vitro. (biomedcentral.com)
  • DMH-1 is a selective inhibitor of bone morphogenic protein (BMP) type-I receptor activin receptor-like kinase 2 (ALK2) receptor (IC 50 = 108 nM or 12.6 nM in in vitro kinase assays). (tocris.com)
  • DMH-1 suppresses lung cancer cell proliferation, migration, invasion in vitro and reduces tumor growth in a mouse lung cancer xenograft model. (tocris.com)
  • By interrogating microarray data from neuronal cells treated with inhibitors of different specificity, we selected two genes encoding histone macroH2A ( H2AFY2 ) and Polycomb group ring finger 2 ( PCGF2 ) that were specifically down-regulated by the inhibitors targeting HDACs1 and 3 versus the more selective inhibitors for further investigation. (frontiersin.org)
  • Our results shed light on the mechanism whereby HDAC inhibitors increase FXN mRNA levels in FRDA neuronal cells. (frontiersin.org)
  • TWS 119 induces neuronal differentiation in pluripotent murine embryonal carcinoma cells and embryonic stem cells at 400 nM. (tocris.com)
  • Hence, ACM improves neuronal maturation principally by amplification of an endogenous pathway which is dependent on GABAA receptor-induced depolarization and consequent Ca2+ inflow. (ack1inhibitor.com)
  • After differentiating the mutant and corrected cells into lung organoids, we show expression of SFTPB mRNA during endodermal and organoid differentiation but the protein product only after organoid differentiation. (nature.com)
  • We also show the presence of normal lamellar bodies and the secretion of surfactant into the cell culture medium in the organoids of lentiviral infected cells. (nature.com)
  • Although many differentiation protocols in the literature have been successful in mimicking lung development from stem cells, there has not been an examination of how a specific mutation impacts the differentiation process including its effects on the early endoderm, as well as the proximal and distal lung epithelial cell populations in the lung organoids. (nature.com)
  • Which cells make NGF and angiotensin in painful murine OA? (ox.ac.uk)
  • We have established RNAScope in murine knee joints which allows one to identify expression of specific single RNA molecules (NGF, angiotensinogen) in individual cells. (ox.ac.uk)
  • RNAScope combined with immunohistochemistry of murine joint sections to visualise cells where NGF and angiotensin are regulated during the development of painful OA. (ox.ac.uk)
  • Here, we present a quantitative and comprehensive study of the abundance levels of histone PTMs during the differentiation of mouse embryonic stem cells (ESCs) using mass spectrometry (MS). We observed dynamic changes of histone PTMs including increased H3K9 methylation levels in agreement with previously reported results. (biomedcentral.com)
  • Inhibition of Brd4:histone H4 interaction by the BET domain inhibitor (+)-JQ1 in ESCs results in enhanced differentiation to the endodermal lineage, by disrupting the protein abundance dynamics. (biomedcentral.com)
  • Embryonic stem cells (ESCs) are self-renewing, pluripotent cells derived from the inner cell mass (ICM) of a blastocyst [ 1 , 2 ]. (biomedcentral.com)
  • Then, typically, genes for transcription factors, are delivered by viral vectors into the cell nuclei, where they are incorporated into the genome. (jove.com)
  • CSR leads to irreversible IgH locus deletions wherein the IgM-producing Cμ exons are permanently excised from the B-cell genome. (northwestern.edu)
  • Both crRNA-tracrRNA duplexes and sgRNAs can be used to target SpCas9 for multiplexed genome editing in eukaryotic cells 1 , 3 . (cdc.gov)
  • The Scientist brings together a panel of experts to educate you about the successes and caveats of using iPSC-derived cells in toxicological assays. (the-scientist.com)
  • In bringing its expertise to Lake County, ARTEC increases its attractiveness for companies that are looking for iPSC-derived cell technologies to advance their gene target identification, drug screening, and disease modeling research. (lakecountypartners.com)
  • These benefits, plus reduced steatosis, were recapitulated by TLC-065 treatment in steatotic, induced pluripotent stem cell (iPSC)-derived HLOs, in which ACMSD expression is upregulated. (orsobio.com)
  • The primary microRNA (Pri-miRNA) is produced in the cell nucleus through the transcription of a DNA strand mediated by RNA polymerase II [ 2 ]. (archivesofmedicalscience.com)
  • A microRNA strand is transferred to the Argonaute complex (AGO), forming an RNA-induced silencing complex (RISC), and guides it to pair with the target mRNA through binding of the microRNA seed sequence with the microRNA recognition site on the mRNA. (archivesofmedicalscience.com)
  • Promising new technologies with potential for corneal wound Fenoterol healing manipulation including microRNA, induced pluripotent stem cells to generate corneal epithelium, and nanocarriers for corneal drug delivery are discussed. (cancerrealitycheck.com)
  • Here we describe how microRNA-regulated lentiviral vectors can be used to visualize specific cell populations by exploiting endogenous microRNA expression patterns. (lu.se)
  • When a microRNA is present in the cell, it binds to the target sites and downregulates GFP expression, while in cells that do not express the microRNA GFP, it is expressed. (lu.se)
  • AChE inhibition induces an acetylcholine build-up, disrupting signals between nerves and muscles, which in high doses leads to asphyxiation. (cdc.gov)
  • The most well-known type of pluripotent stem cell is the embryonic stem cell. (wikipedia.org)
  • Patient-matched embryonic stem cell lines can now be derived using somatic cell nuclear transfer (SCNT). (wikipedia.org)
  • They hypothesized that genes important to embryonic stem cell (ESC) function might be able to induce an embryonic state in adult cells. (wikipedia.org)
  • The ability to take a skin cell and reprogram it into an induced pluripotent stem cell, a cell theoretically capable of generating any cell in the body, provides an invaluable tool for studying such lung diseases. (ca.gov)
  • However, scientists have found a way to reprogram these mature cells so that they "de-differentiate" and return to an unspecialized, proliferative state. (jove.com)
  • A Harvard Medical School team led by Fulcrum Therapeutics Inc. (NASDAQ:FULC) co-founder Jeannie Lee revealed in Cell that MEK and BRAF inhibitors together elicited CGG repeat contraction and full FMR1 reactivation in an induced pluripotent stem cell (iPSC) line from a male fragile X syndrome patient with a full mutation, defined as greater than 200 copies of the CGG repeat. (biocentury.com)
  • However, only compounds targeting the class I HDACs 1 and 3 are active in increasing FXN mRNA in these cells. (frontiersin.org)
  • Structural analogs of the active HDAC inhibitors that selectively target either HDAC1 or HDAC3 do not show similar increases in FXN mRNA levels. (frontiersin.org)
  • MicroRNAs regulate gene expression at the post-transcriptional level by binding to 3′- or less often to 5′-untranslated regions of target messenger RNAs (mRNAs), which in consequence leads to inhibited translation and/or induces degradation of targeted mRNA [ 1 ]. (archivesofmedicalscience.com)
  • Robust multiciliogenesis occurred when notch signaling was inhibited and was confirmed by (i) the assembly of multiple pericentrin-stained centrioles at the apical surface, (ii) expression of transcription factor forkhead box protein J1, and (iii) presence of multiple acetylated tubulin-labeled cilia projections in individual cells. (ca.gov)
  • Oxidative stress was markedly attenuated by iPS-MSCs both in AN mice and in protein-overloaded tubular cells. (oncotarget.com)
  • Wu then performed immunoprecipitation experiments, which use antibodies to identify protein interactions in the cell nucleus. (pharmaceuticalintelligence.com)
  • To understand the mechanism of action of these compounds, we probed the kinetic properties of the active and inactive inhibitors, and found that only compounds that target HDACs 1 and 3 exhibited a slow-on/slow-off mechanism of action for the HDAC enzymes. (frontiersin.org)
  • Alveolar type II (ATII) epithelial cells synthesize, secrete and recycle all components of surfactant and dysfunction in surfactant metabolism can result in a variety of pediatric lung diseases including respiratory distress syndrome and interstitial lung disease 3 . (nature.com)
  • We developed a step-wise differentiation protocol ending in an air-liquid interface to generate a pseudostratified polarized layer of endodermal-derived epithelial cells, the cells that reside in the respiratory tract. (ca.gov)
  • Clara, goblet, and basal cells were all present, confirming the generation of a complete polarized epithelial-cell layer. (ca.gov)
  • Additionally, cAMP-activated and cystic fibrosis transmembrane regulator inhibitor 172-sensitive cystic fibrosis transmembrane regulator currents were recorded in isolated epithelial cells. (ca.gov)
  • Attention is also paid to problems in wound healing understanding and treatment, such as lack of specific epithelial stem cell markers, reliable identification of stem cells, efficient prevention of haze and stromal scar formation, lack of data on wound regulating microRNAs in keratocytes and endothelial cells, as well as virtual lack of targeted systems for drug and gene delivery to select corneal cells. (cancerrealitycheck.com)
  • 2013) and in primary limbal epithelial cells (Winkler et al. (cancerrealitycheck.com)
  • 2010). Several studies also suggest that it stimulates better wound healing responses than EGF in corneal epithelial cells (Xu et al. (cancerrealitycheck.com)
  • Using these cells, and established high throughput assays, we will assess the ability of NGF and angiotensin, either alone or in combination, and in the presence or absence of inhibitors, to activate nociceptors. (ox.ac.uk)
  • High-content assays for hepatotoxicity using induced pluripotent stem cell-derived cells. (tamu.edu)
  • QuinoMit Q10-Fluid attenuates hydrogen peroxide-induced irregular beating in mouse pluripotent stem cell-derived cardiomyocytes. (uni-koeln.de)
  • Particularly, non-hematopoietic cells, such as fibroblasts and cardiomyocytes, differentiated from autoimmune patient-derived iPS cells have shown promising results for further research into the pathogenesis. (biomedcentral.com)
  • Endothelial cells (ECs) and endothelial progenitor cells (EPCs) play crucial roles in maintaining vascular health and hemostasis. (nih.gov)
  • TWS 119 enhances the proliferation and survival of γδ T-cells, and induces differentiation of CD8+ T cells towards a stem cell-like memory T cell phenotype at 2.5 μM. (tocris.com)
  • In acute myeloid leukemia (AML), malignant transformation and uncontrolled proliferation of an abnormally differentiated, long-lived myeloid progenitor cell results in high circulating numbers of immature blood cells and replacement of normal marrow by malignant cells. (msdmanuals.com)
  • The galactose‑1‑phosphate concentration was significantly higher in cells cultured in HDI than in those cultured with ReproFF. (spandidos-publications.com)
  • Advancing your pluripotent stem cell therapy research to clinical applications requires careful material selection as the quality of starting materials significantly impacts the properties of your final stem cell therapy product. (thermofisher.com)
  • In a cohort study of 27 HGPS patients treated with the farnesyltransferase inhibitor lonafarnib, compared with a pool of 103 contemporaneous untreated patients, treatment with lonafarnib monotherapy was associated with a significantly lower mortality rate (3.7% versus 33.3%) after a median 2.2 years of follow-up. (aad.org)
  • Both 15-second and 2-minute exposures induced phase delay shifts [median (± SD)] of -34.8 ± 47.2 minutes and -45.4 ± 28.4 minutes, respectively, that were significantly ( P = 0.04) greater than the control condition (advance shift: +22.3 ± 51.3 minutes) but were not significantly different from each other. (jci.org)
  • Mast cells play an important role in a variety of biological processes including allergic reactions, atherosclerosis and inflammation 7,8 . (bvsalud.org)
  • As far as we know, we are the only ones in the Chicago-area with the expert knowledge to produce vascular and blood cells from iPSC. (lakecountypartners.com)
  • The activation of these cells leads to the release of chemical mediators such as histamine and arachidonic acid metabolites, which increase vascular permeability and tissue swelling 9 . (bvsalud.org)
  • The ability to generate isogenic lines has eliminated the clonal heterogeneity observed when comparing cell lines of different genetic backgrounds during differentiations to derivative tissues and downstream functional applications. (chop.edu)
  • ARTEC's technology allows the introduction of genetic modifications at the iPSC level, and then differentiating them to adult cell types. (lakecountypartners.com)
  • Since autoinflammatory diseases are usually monogenic, genetic mutations affect the cell function and patient-derived iPS cells tend to exhibit disease-specific phenotypes. (biomedcentral.com)
  • In particular, patient-derived iPS cells retain the same genetic background as these patients, allowing for recurrent analysis of genetic effects on cellular functions. (biomedcentral.com)
  • Cell Biol Int;46(10): 1625-1636, 2022 Oct. (bvsalud.org)
  • Upon introduction of reprogramming factors, cells begin to form colonies that resemble pluripotent stem cells, which can be isolated based on their morphology, conditions that select for their growth, or through expression of surface markers or reporter genes. (wikipedia.org)
  • The transcription factors then turn on genes that are expressed by embryonic stem cells. (jove.com)
  • Then, genes for multiple transcription factors are delivered into the cells using a viral vector, and the transcription factor proteins are expressed using the cell's machinery. (jove.com)
  • The transcription factors then turn on many other genes that are expressed by embryonic stem cells, returning the cells to an undifferentiated, proliferative, and pluripotent state. (jove.com)
  • The Ccr7, Slfn1, and Mapk11 genes were weakly induced in mature cell populations from only one of the mutant mice, but remained at background levels across all populations in the samples derived from the second mouse. (jak-inhibitors.com)
  • with the previous results demonstrating impaired expression of genes associated with positive selection in DP cells from Bcl11b-deficient thymocytes 26. (jak-inhibitors.com)
  • Collectively, these analyses indicate that the premature expression of SP-associated genes in Bcl11bdp−/− DP cells reflects gene-specific dysregulation in cells that have not undergone positive selection. (jak-inhibitors.com)
  • These data indicate that Bcl11b likely acts directly in DP cells to prevent the premature expression of genes encoding critical regulators of the SP differentiation programs. (jak-inhibitors.com)
  • In yet another coup for a research concept known as "big data," researchers at the Stanford University School of Medicine have developed a computerized algorithm to understand the complex and rapid choreography of hundreds of proteins that interact in mindboggling combinations to govern how genes are flipped on and off within a cell. (pharmaceuticalintelligence.com)
  • At the end of 2023, researchers at Lund Stem Cell Center received funding amounting to SEK 77.5 million from the Swedish Research Council, the Swedish Cancer Society, and the Swedish Childhood Cancer Fund. (lu.se)
  • At Lund Stem Cell Center, researchers are using innovative approaches to advance the field, offering exciting possibilities for the future. (lu.se)
  • We hope that this new method will foster a culture of proactive health management and shift the paradigm towards preventive care, in addition to paving the way for more effective treatments," says Sandra Linstedt, research group leader at Lund Stem Cell Center. (lu.se)
  • Our CTS products have been used in over 200 clinical trials as well as in commercially approved cell therapies including FDA-approved CAR-T therapies and the first FDA-approved therapeutic cancer vaccine. (thermofisher.com)
  • Moreover, iPS cell-based studies have proposed new diagnostic biomarkers and contributed to the discovery of new therapeutic targets [ 7 ]. (biomedcentral.com)
  • I see tremendous therapeutic potential in OrsoBio's ACMSD inhibitors across a spectrum of metabolic and inflammatory hepatic and renal diseases. (orsobio.com)
  • It is also clear that pro-mitotic factors such as C-MYC/L-MYC or repression of cell cycle checkpoints, such as p53, are conduits to creating a compliant cellular state for iPSC reprogramming. (wikipedia.org)
  • The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
  • T cell responses. (jak-inhibitors.com)
  • Since mast cells are active during inflammatory responses it becomes crucial to find out if these cells are actually present and play a role in dental pulp under normal and inflammatory conditions either in humans or in rats. (bvsalud.org)
  • Because they can propagate indefinitely, as well as give rise to every other cell type in the body (such as neurons, heart, pancreatic, and liver cells), they represent a single source of cells that could be used to replace those lost to damage or disease. (wikipedia.org)
  • Disease modeling studies using induced pluripotent stem cells: are we using enough controls? (mayo.edu)
  • This treatment avoids the common harsh side effects of current standard of care treatments and the risk of Graft Vs. Host Disease (GVHD) associated with newer cancer treatments like CAR-T. It is the first "off-the-shelf" live NK cell therapy developed from induced pluripotent stem cells (iPSC) that does not require co-administration of check-point inhibitors to improve efficiency. (lakecountypartners.com)
  • The new cells could then be transplanted back into the patient to treat damage or disease with minimal risk of rejection, because they originated from the patient's own cells. (jove.com)
  • On the other hand, the utilization of disease-specific iPS cells is less successful for research on autoimmune diseases. (biomedcentral.com)
  • Nevertheless, several groups have reported studies utilizing autoimmune disease patient-derived iPS cells, including patients with rheumatoid arthritis, systemic lupus erythematosus (SLE), and systemic sclerosis. (biomedcentral.com)
  • Neurons and glia are commonly affected, likely because of the relative paucity of cell turnover in the central nervous system, yet non-neuronopathic forms of lysosomal storage disease exist. (medscape.com)
  • These unlimited supplies of autologous cells could be used to generate transplants without the risk of immune rejection. (wikipedia.org)
  • This layer includes secretory cells called Clara cells and goblet cells and multiciliated cells which generate flow in the fluid lining the lungs. (ca.gov)