• Increases in the amount of PAPP-A mRNA in granulosa cells during follicular development occurs in some but not all species, indicating that other proteases or protease inhibitors may be involved in IGFBP degradation. (bioone.org)
  • In Experiments IV and V, three protease inhibitors, namely 6-dimethylaminopurine (6-DMAP, 2 mM), cycloheximide (7.5 microg/ml) and cytochalasin B (7.5 microg/ml), were used as in Experiment II. (druglib.com)
  • Results: APAP-induced upregulation of ferroptosis, levels of lipid peroxides and reactive oxygen species, and depletion of glutathione were effectively alleviated by the ferroptosis inhibitor, ferrostatin-1, and downregulation of GAS1 expression. (bvsalud.org)
  • Camptothecin, an inhibitor of nuclear topoisomerase, induces apoptosis in many types of cells. (enzolifesciences.com)
  • IL-1 beta also induces the accumulation of cGMP by the insulinoma cell line Rin-m5F, and both NMMA as well as the protein synthesis inhibitor cycloheximide prevent this cGMP accumulation. (jci.org)
  • It was discovered that while GH induces the transcription of genes such as c-fos, c-jun, insulin-like growth factor-I, and serine protease inhibitor 2.1, it resulted in the acute inhibition of insulin-like growth factor binding protein-1 and albumin gene expression. (aacc.org)
  • A study was conducted to determine the effects of pretreatment at varying concentrations of Cycloheximide (CHI) on senescence and postharvest performance in Nerine sarniensis . (scialert.net)
  • The present study was undertaken to investigate the effects of pretreatment with different concentrations of Cycloheximide (CHI) before transfer to Distilled Water (DW) or Sucrose (0.15 M) on senescence with the ultimate aim to gain an insight into the mechanism of senescence and to improve its postharvest performance. (scialert.net)
  • Pretreatment of beta-cells, purified by FACS with IL-1 beta results in a 40% inhibition of glucose-stimulated insulin secretion that is prevented by the nitric oxide synthase inhibitor, NG-monomethyl-L-arginine (NMMA). (jci.org)
  • Cycloheximide or Chk2 inhibitor pretreatment abrogated not only activation of Chk2 but also G 2 /M arrest and apoptosis mediated by DIM. (aspetjournals.org)
  • Pretreatment with transcriptional (actinomycin D), translational (cycloheximide) or HSF1 (KNK437) inhibitors prevented the ITC-induced accumulation of HO-1, HSP70 and HSP30. (uwaterloo.ca)
  • This possibility was supported by the finding that BITC pretreatment reduced the formation of aggresome-like structures induced by the proteasomal inhibitor, MG132. (uwaterloo.ca)
  • The other elongating regions can be detected by adding antibiotics like cycloheximide that inhibit translocation, chloramphenicol that inhibits transfer of peptides within the ribosome, or non-drug means like thermal freezing. (wikipedia.org)
  • Cycloheximide, an active antibiotic against many yeast and fungi, inhibits protein synthesis. (enzolifesciences.com)
  • α Hydrazino ornithine is a potent and selective inhibitor of ornithine decarboxylase (L ornithine carboxy lyase). (johnshopkins.edu)
  • Protein stability was determined following treatment with protein synthesis inhibitor cycloheximide and proteasome inhibitor MG132. (thno.org)
  • Inhibition of VCP either by genetic depletion or the pharmacologic inhibitor CB-5083 increased ubiquitination and degradation of p53-R273H, leading to cell death. (aacrjournals.org)
  • The present study was designed to examine the underlying mechanism(s) by which CKS-17 down-regulates the TNF-alpha mRNA expression using a human acute monocytic leukemia cell line THP-1 stimulated with the superantigen staphylococcal enterotoxin E. A cyclooxygenase inhibitor indomethacin does not reverse the inhibition of TNF-alpha mRNA expression by CKS-17, suggesting that prostaglandins are not responsible for the suppressive action of CKS-17. (duke.edu)
  • To determine whether Met expression levels correlate with sensitivity to Met inhibition by tyrosine kinase inhibitors (TKIs), we performed cell viability studies. (thno.org)
  • When added to rat hepatoma cells at the time of dilution, α hydrazino ornithine elicits a dose related increase in ornithine decarboxylase activity and a concomitant prolongation of the apparent half life of the enzyme from 10 min to 28 min, as determined by the rate of decline of ornithine decarboxylase activity after inhibition of protein synthesis by cycloheximide. (johnshopkins.edu)
  • The present invention provides a method for treating SWI/SNF complex-deficient cancers comprising a glutathione (GSH) metabolic pathway inhibitor, a pharmaceutical composition comprising a glutathione (GSH) metabolic pathway inhibitor for therapy in SWI/SNF complex-deficient cancers, and a glutathione (GSH) metabolic pathway inhibitor for use in treating SWI/SNF complex-deficient cancers. (justia.com)
  • The present invention also provides a method for detecting and/or selecting a susceptible patient to a glutathione (GSH) metabolic pathway inhibitor. (justia.com)
  • The present invention relates to a method for treating SWI/SNF complex-deficient cancers comprising a glutathione (GSH) metabolic pathway inhibitor, a pharmaceutical composition comprising a glutathione (GSH) metabolic pathway inhibitor for therapy in SWI/SNF complex-deficient cancers, and a glutathione (GSH) metabolic pathway inhibitor for use in treating SWI/SNF complex-deficient cancers. (justia.com)
  • Cycloheximide reduced both aldosterone- and dexamethasone-induced TH mRNA. (elsevierpure.com)
  • Cycloheximide can be used to study cellular processes and inhibit yeast growth. (goldbio.com)
  • Moreover, inhibitor of PI3K/AKT signaling pathway could inhibit paraptosis-like cell death induced by 125 I seeds radiation. (hindawi.com)
  • Since epidermal growth factor receptor (EGFR)-tyrosine kinase inhibitors, including gefitinib (GEF) have been reported to induce the apoptosis of several cancer cell lines, in the present study, we examined whether the cytotoxic effects of GEF are further enhanced under amino acid starvation (AAS) culture conditions. (spandidos-publications.com)
  • The insulin-induced increases in both ET-1 mRNA and ET-1 protein secretion were significantly attenuated by genistein, a tyrosine kinase inhibitor. (diabetesjournals.org)
  • While initially received with enthusiasm, results from targeted therapy with small molecule tyrosine kinases inhibitors (TKIs) have been mixed, in part due to poor patient selection and compensatory changes in signaling networks upon blockade of one or more kinase of tumors. (thno.org)
  • The interactions between proteases and their inhibitors have been linked to modulation of cell death processes in plants and in certain cut flowers. (scialert.net)
  • The fact that cycloheximide delays tepal senescence demonstrates the synthesis of particular proteins probably enzymes, responsible for degradation of cellular constituents, executes the cell death programme in flower tepals. (scialert.net)
  • This protection was blocked if cells were treated with the protein synthesis inhibitor cycloheximide. (cdc.gov)
  • Cycloheximide is a protein synthesis inhibitor in eukaryotic cells. (goldbio.com)
  • These observations are consistent with a model in which 6AN and other inhibitors of protein synthesis act as modulating agents by increasing cisplatin accumulation, thereby enhancing the formation of Pt-DNA adducts and subsequent cisplatin-induced cell death. (aspetjournals.org)
  • Furthermore, studies using cycloheximide indicate that these GH-induced changes in mRNA expression do not require protein synthesis and hence are primary, not secondary events. (aacc.org)
  • Immediate post-training infusions of the protein synthesis inhibitor, cycloheximide, targeting the basal nucleus of the amygdala (BA) attenuated trace and contextual fear memory expression, but had no effect on delay fear conditioning. (ed.gov)
  • Methods: Cyclohexamide, an inhibitor of protein synthesis, was used to investigate the stability of CD86 on the surface of human APCs and MARCH 1 and 8 ligases were overexpressed in Chinese Hamster Ovary (CHO)-CD86 cells. (bham.ac.uk)
  • The inhibitory effect of CKS-17 is, however, significantly blocked by a protein synthesis inhibitor cycloheximide, indicating that CKS-17 requires de novo protein synthesis to induce the suppressive activity. (duke.edu)
  • Nuclear movement and flagellar root contraction and extension are not sensitive to inhibitors of protein synthesis (cycloheximide), or drugs that influence either microtubules (colchicine) or actin-based microfilaments (cytochalasin D). Detergent-extracted cell models contract and extend their flagellar roots and move their nuclei in response to alterations of free calcium levels in the medium. (rupress.org)
  • Ferroptosis 과정에 관여하는 inhibitor와 activator를 소개합니다. (komabiotech.co.kr)
  • The presence of necrostatin-1, an inhibitor of receptor-interacting serine/threonine-protein kinase 1 (RIPK‑1), but not that of Z-VAD-fmk, attenuated the cytotoxic effects of GEF under AAS culture conditions. (spandidos-publications.com)
  • The harvested scapes were immediately brought to the laboratory, cut to a uniform size of 30 cm and pulse treated for 1 h separately with varying concentrations of cycloheximide (0.01, 0.05, 0.1 and 0.5 mM). (scialert.net)
  • and 2) simultaneous treatment of cells with 6AN and cycloheximide prevented the increase in GRP78 but not the sensitizing effect of 6AN. (aspetjournals.org)
  • Upon addition of cycloheximide, WT-TRPM4 decayed with a half-life of ~20 h, while loss-of-expression variants showed a ~30% increase in degradation rate, with a half-life close to 12 h. (frontiersin.org)
  • Although many studies on RCC have demonstrated the importance of MMPs, very little information is currently known regarding the effect of inducers and inhibitors. (drrathresearch.org)
  • All the inhibitors tested without and with PMA showed a dose-dependent decrease in both MMP-2 and -9 expression. (drrathresearch.org)