Infection bacterial isolation microbial viability. Medical search. Frequent questions

Nucleic acid amplification diagnostics offer a rapid and accurate method of diagnosing microbial pathogens. (medscape.com)
It has been used for the diagnosis of multiple viral and bacterial pathogens, including H influenzae . (medscape.com)
Thus, allows the recovery of new bacterial species from heavily virulent capsular strains were phagocytosed by and repli- contaminated samples and might be a valuable approach cated in Acanthamoeba castellanii , leading to amoebal for the recovery of as-yet unrecognized emerging death, while the nonvirulent acapsular strains were killed pathogens from clinical specimens. (cdc.gov)
They represent the first line of defense against pathogens in the lower airspace and recognize microbial ligands via pattern recognition receptors ( 4 , 5 ). (frontiersin.org)
A Monitoring for human pathogens in sewage Monitoring of human pathogens in sewage is possible because they may be excreted in a range of bodily fluids, skin, and hair during active infection (Feachem et al. (scienceopen.com)
In addition to release during active infection, pathogens can be washed into sewage systems from cleaning of indoor (floor washing, kitchen sink use) and outdoor (auto washing, driveway cleaning, storm water collection) facilities. (scienceopen.com)
Control of such infections is hampered by persistent contamination of foods and food-processing environments, resistance of pathogens to sanitizing agents, existence of heterogeneous populations of pathogens (including culturable and viable but non-culturable cells) within the same food items, and inability to detect all such pathogens by culture-based methods. (biomedcentral.com)
Candida albicans and Staphylococcus aureus , representing two different kingdoms, are the most frequently isolated pathogens from invasive infections. (mdpi.com)
In other bacterial pathogens, genes important for pathogenicity and host range include secreted protein effectors that suppress host immunity, alter host metabolism, and enable colonization, providing a fitness advantage. (biorxiv.org)

This is particularly relevant for bacterial skin and soft-tissue infections (SSTIs) being most commonly caused by multidrug-resistant bacteria with Staphylococcus aureus as predominant causative Gram-positive bacterium and Pseudomonas aeruginosa as most frequent Gram-negative bacterium and having fatal consequences if treated unproperly 2 . (nature.com)
Strains of Staphylococcus aureus (SA), Escherichia coli (EC) and Candida albicans (CA) were used to construct single-surface, full-surface, and internal infection models in sterile pork tissue. (researchsquare.com)
The strain of Staphylococcus aureus ( S. aureus ) ATCC 25923 (American Type Culture Collection, USA) the strain of Escherichia Coli ( E. coli ) ATCC 25922 (American Type Culture Collection, USA), and the strain of Candida albicans ( C. albicans )ATCC 90029 (American Type Culture Collection, USA) were used to establish infection model in vitro. (researchsquare.com)

The target of the nanobodies was identified as the major outer membrane protein, a porin that contributes to bacterial virulence and viability. (biomedcentral.com)

It is usually the causative agent of the relatively rare bacterial disease listeriosis, an infection caused by eating food contaminated with the bacteria. (wikipedia.org)
Chemical interaction with matrix molecules is hypothesized, based on SEM images and considering the low or absent anti-microbial activity on planktonic bacteria showed by flow cytometry. (frontiersin.org)
Antimicrobial peptides are promising agents for the treatment of bacterial infections and recent studies indicate that Pep19-2.5, a synthetic anti-lipopolysaccharide (LPS) peptide (SALP), efficiently neutralises pathogenicity factors of Gram-negative (LPS) and Gram-positive (lipoprotein/-peptide, LP) bacteria and protects against sepsis. (nature.com)
Microbial-synthesized silver nanoparticles have a potential application to combat multidrug-resistant bacteria, especially in the intensive care unit. (springeropen.com)
Antibiotic-resistant bacteria are a growing problem in the 21st century, causing longer and more serious infections, and resulting in poor outcomes for vulnerable patients. (qub.ac.uk)
For many bacteria, biofilm formation is a key part of their pathogenic behaviour, and is especially important in the development of medical device-associated infection. (qub.ac.uk)
Our 'A40-Micro' flow cytometer gives the performance we need for measuring small bacteria, assessing their viability and absolute count. (apogeeflow.com)
These bacteria might cause secondary infections to animals, so the parasite can become a vector of pathogenic bacterial, reason why the understanding of the 'bacteria-parasite interaction" is important to combating of parasitic infestations. (fortunepublish.com)
Understand the bacteria-parasite interaction will help us to the control of parasitic diseases in an integral form, as well know as the bacterial infections that can develop from the parasitic infestation. (fortunepublish.com)
The use of antagonist bacterial is a biotechnological option in the control of parasites as therapy on the animal biomedicine, so in this review, we have a general idea of the "bacteria-parasite interaction' and their impact on the animal's health. (fortunepublish.com)
It should be noted the keep symbiotic bacteria and parasites interaction can become an alternative method in therapies dewormers dependent on the presence or absence of the bacterial biota. (fortunepublish.com)
Reduced microbial diversity and altered function in the GI tract as a result of antibiotic exposure may contribute to the proliferation of pathogenic bacteria leading to enteric infections such as Clostridioides (previously Clostridium) difficile infection (CDI) and recurrence of CDI (rCDI) 1 , 8 . (dunianusantara.io)
After biomechanical preparation of root canals, the bacterial load is reduced by at least 95% and the remaining viable bacteria may be the cause of refractory lesions 8 . (bvsalud.org)

Owing to the alarming increase in bacterial resistance towards conventional antibiotics and the decrease in the development of new antibiotics at the same time, treatment of bacterial infections has become a major clinical problem 1 . (nature.com)
Antibiotics for bacterial infections. (stemcelldaily.com)
Antibiotics used to treat C. difficile infection are usually broad spectrum and can further disrupt the commensal gut microbiota, leaving patients susceptible to recurrent C. difficile infection. (dunianusantara.io)
The authors used data from a trial of antibiotics for foot infections in diabetic patients (SIDESTEP) and evaluated the accuracy of their wound-scoring technique in predicting outcome. (medscape.com)

Previous issue date: 2014-01-30 / This study aims to evaluate the microbiota of necrotic primary teeth through the technique of polymerase chain reaction (PCR), whereas primary endodontic infections, comparing the microbiota of necrotic caries and dental trauma. (ndltd.org)
In cases of primary endodontic infections, there is a higher bacterial load than in secondary infections 6-7 . (bvsalud.org)

Dr. O'Rourke and her team found that isolates for both species, B. cepacia and B. contaminans recovered from the ISS PWD had highly similar genomes regardless of isolation date. (jcvi.org)
The resolution of variation within species is critical for interpreting and acting on many microbial measurements. (bvsalud.org)
The most common species of human infection is P. aeruginosa , which can cause infections in the blood, lungs (pneumonia), and skin, particularly in the immunocompromised patients post-surgery. (springeropen.com)
A variety of enrichrnent and selective culture media that enable distinction between pathogenic and non-pathogenic Listeria species have been formulated for isolation of Listeria spp. (biomedcentral.com)
In Africa, Combretum species are well known in traditional medicine and used for various ailments and diseases ranging from heart and worm remedies to wound dressings, treatment of the mentally ill, scorpion stings and snake bites, to fever and microbial infections [ 9 ]. (biomedcentral.com)

This study also evaluated the bacterial viability in three transport medias (Reduced to Fluid Transport , Tris - EDTA and Viability Medium Göteborg anaerobically III), simulating the conditions of carriage of the endodontic microbiota from the clinic to the laboratory, considering the time elapsed between the collection and processing. (ndltd.org)
Este trabalho também avaliou a viabilidade bacteriana em três meios de transporte (Fluido Reduzido para Transporte, Tris- EDTA e Viability Medium Göteborg Anaerobically III), simulando as condições de transporte da microbiota endodôntica da clínica ao laboratório, considerando o tempo decorrido entre a coleta e o processamento. (ufc.br)

The detection of microbial molecules via Toll-like receptors (TLR) in B cells is not well characterized. (aai.org)
The Veriflow system utilizes a game-changing technology that combines proven diagnostic principles for microbial detection with innovative, first-in-class scientific approaches. (invisiblesentinel.com)
Veriflow's proprietary DNA Signature Capturing technology combines proven diagnostic principles for microbial detection with innovative, first-in-class scientific approaches for unparalleled performance. (invisiblesentinel.com)

In this study, we describe the isolation and characterization of nanobodies (Nb) recognizing multiple Campylobacter strains. (biomedcentral.com)

In man-made water systems microbial biofilms increase the resistance of legionella to disinfection, posing a significant threat to public health. (frontiersin.org)
For microbiological collection after infiltration anesthesia and absolute isolation, disinfection of the operative field to open the coronary cavity was performed. (ndltd.org)
Terminal disinfection and daily cleaning have been performed in hospitals in Taiwan for many years to reduce the risks of healthcare-associated infections. (microbe.net)
Here, we report the surface changes of bacterial communities with terminal disinfection and daily cleaning in a medical intensive care unit (MICU) and only terminal disinfection in a respiratory care center (RCC) using 16s ribosomal RNA (rRNA) metagenomics. (microbe.net)

Antifungals for fungal infections. (stemcelldaily.com)
Since 1970s, the emergence of antifungal drug resistance has been a cause of concern in the treatment of invasive fungal infections such as candidiasis [ 1 ]. (biomedcentral.com)
Persons who are at high risk for systemic fungal infections include those who have haematological malignancies, undergoing prolonged bone marrow transplantation and chemotherapy, HIV infected patients and severely burnt patients [ 3 ]. (biomedcentral.com)

ABSTRACT The study aimed to develop a rapid and simple method for the primary isolation and de- tection of Helicobacter pylori from dyspeptic patients. (who.int)

However, laboratory and WGS methods depend on an isolation step that is time-consuming and incompletely represents the sample when multiple strains are present. (bvsalud.org)
Identifying novel antimicrobial agents from plant-based compounds may result in anti-microbial drugs effective against the resistant strains. (biomedcentral.com)
Viable E. faecalis strains could be trapped inside the root canal in the filling material and provide a niche for subsequent infection in the long term 13 . (bvsalud.org)

The leaves of Combretum zeyheri have been used as herbal medicine and have been reported to have pharmacological activity which includes anti-bacterial, anti-fungal, anticancer and antioxidant properties. (biomedcentral.com)
As a result, R. chinensis is a good candidate to examine for in vitro anti-bacterial and anti-proliferative properties. (biomedcentral.com)
Anti-Bacterial Agents. (bvsalud.org)

Community sequencing approaches that skip the isolation step are therefore of interest for pathogen surveillance. (bvsalud.org)

Microbial Community and Potential Pathogen Shifts Along an Ornamental Fish Supply Chain. (mbl.edu)
In order to simulate PJI infection as much as possible, in which pathogen may be distributed on a single surface or multiple surfaces even inside tissues 6 , therefore, three tissues infection models were established ( Fig 1): ① Single surface infection: pipette aspirate 10 μL (200 CFU) bacterial dilution ( S. aureus /E. coli/Candida albicans ) to colonized a single surface of the pork cube. (researchsquare.com)

During retreatment, a microbial sample was obtained from the root canal and inoculated in a selective Enterococcus medium. (bvsalud.org)
Using bacterial cultivation methods, bacterial isolates belonging to the genus Enterococcus were identified. (bvsalud.org)

preventive actions and their effectiveness allowed us to intercept microbiological SE and trigger appropriate precautionary behaviour and isolation. (microbe.net)

Microbial biosynthesis of the AgNPs using the supernatant is an easy and cheap method to synthesize NPs with specific size and morphology shapes. (springeropen.com)
Microbial products such as enzymes, proteins, and bio-surfactants are the key to capping and/or stabilizing of the NPs in the biosynthesis process [ 4 ]. (springeropen.com)

from urine cultures of dogs with urinary tract infections (UTIs). (bvsalud.org)
from canine urinary tract infections. (bvsalud.org)
Escherichia coli which causes urinary tract infections. (stemcelldaily.com)

Thus it is concluded from clinical samples of necrotic primary teeth, the methodology involved in the bacterial collection and storage process as the means of transport, can influence our findings. (ndltd.org)
According to the findings, M. incognita infection can alter the bacterial population in the soil. (ppjonline.org)

Bacterial ability to form biofilms was verified using a crystal violet colorimetric assay and testing cell viability by real-time quantitative PCR and Plate Count assay. (frontiersin.org)
This is the first report on rapid and efficient synthesis of AgNPs, AuNPs and AgAuNPs from P. zeylanica and their effect on quantitative inhibition and disruption of bacterial biofilms. (dovepress.com)

The skin comprises various cell types providing the potential to react to bacterial pathogenicity factors and can thus initiate inflammatory responses if exposed to these factors 5 . (nature.com)

Many events can be responsible for these soft tissue infections. (medscape.com)
A description of soft tissue infections includes simple, moderate, and severe infections, which includes but is not limited to the immunocompromised patient, infections associated with PVD, emergency soft tissue infections, and infections associated with trauma. (medscape.com)

Infection with a fungus of the genus CANDIDA. (lookformedical.com)
Infection of the mucous membranes of the mouth by a fungus of the genus CANDIDA. (lookformedical.com)
In Chile, there are yet no studies about the presence of this bacterial genus in CAP. (bvsalud.org)

Common bacterial diseases include UTIs, food poisoning, STIs and some skin, sinus and ear infections. (stemcelldaily.com)

The Setting is inpatient care with Multi- Drug-Resistance infections. (microbe.net)
These new antimicrobial and antibiofilm technologies will treat wound and medical device related infections, and will provide an alternative treatment to antibiotic classes in which bacterial resistance is high. (cdc.gov)

For H influenzae infection, PCR is more accurate than conventional methods of serotyping (slide agglutination method). (medscape.com)
This study aimed to evaluate the effects of different pretreatment methods on the microbial yield in infected tissues. (researchsquare.com)
Manual milling (MM), mechanical homogenization (MH), sonificated (SF), dithiothreitol (DTT), and direct culture (DC) were used to pretreat tissues, the microbial yield from different pretreatment methods were compared. (researchsquare.com)
At the same time, periprosthetic tissues collected intraoperatively from periprosthetic joint infection (PJI) patients were pretreated with the same methods. (researchsquare.com)
Moreover, the number of bacterial colonies obtained from periprosthetic tissues pretreated by MH was significantly higher than pretreated by other pretreatment methods ( P = 0.004). (researchsquare.com)
The effects of MH and DTT in microbial yield were significantly higher than that of DC, SF and MM, and these methods can be used to process multiple tissue samples at the same time, which might further improve the sensitivity of infectious disease. (researchsquare.com)
At present, the traditional laboratory diagnostic methods include synovial fluid, sonication fluid and periprosthetic tissues, but in many cases, synovial/sonication are insufficient, resulting in poor sensitivity of microbial culture. (researchsquare.com)
Therefore, in this study, the abovementioned pretreatment methods were used to treat infection tissues in vitro and PJI tissue specimens. (researchsquare.com)
2 methods: histopathological examination tion is focal, negative biopsy results do not and bacterial culture growth. (who.int)

Campylobacter infections are among the most prevalent foodborne infections in humans, resulting in a massive disease burden worldwide. (biomedcentral.com)
Campylobacter remains the leading cause of bacterial foodborne illness in the U.S. and worldwide. (bvsalud.org)

The CDC's (Center for Disease Control) Environmental Legionella Isolation Techniques Evaluation (ELITE) Program was utilized for validation and verification of assay performance at an ELITE member lab. (invisiblesentinel.com)

Overall, the project will develop antimicrobial coatings which can reduce the risk of medical device-related infections. (qub.ac.uk)

The viability of H influenzae is lost rapidly, so clinical specimens should be inoculated to an appropriate culture media without delay. (medscape.com)
Therefore, optimizing the pretreatment method of tissue specimens to make full use of PJI tissue samples is of great significance for improving the sensitivity of microbial culture as a diagnostic tool. (researchsquare.com)
For bacterial culture, specimens were cultured in parallel on 2 media: the non- selective classic Columbia agar and the selective modified Columbia urea agar (MCUA). (who.int)

Multiplex PCR assays are commonly used to evaluate CNS and respiratory tract infections. (medscape.com)

The antimicrobial activity of AgNPs is directly proportional to the surface area of the particles exposed to the interaction, which facilitates the mechanical penetration of the cell membrane and increases the adhesion to the surface of microbial cells better. (springeropen.com)

Antiparasitics for parasitic infections. (stemcelldaily.com)
Parasitic infections are important in the livestock sector and pets because they affect the production and animal welfare, in addition to endangering human health by parasitosis called zoonoses, that generate economic losses focused on its control and prevention [1]. (fortunepublish.com)

After many years of use, we appreciate the high sensitivity not only of the fluorescence signals (for example viability assessment), but also of the light scatter signals. (apogeeflow.com)

Infections caused by multi-drug resistant Escherichia coli cause significant morbidity and mortality especially in developing countries. (bvsalud.org)

The preliminary isolation of C. zeyheri active compounds was carried out using chromatographic techniques which include sephadex gel column chromatography, silica gel column chromatography and thin-layer chromatography (TLC). (biomedcentral.com)

It is usually a superficial infection of the moist areas of the body and is generally caused by CANDIDA ALBICANS. (lookformedical.com)

Last 72 hours counting of bacterial colonies was performed. (ndltd.org)

Antivirals for viral infections. (stemcelldaily.com)

the nutritional role, immunomodulating property, and contribution to the pathogenesis [5-7], at the same time, the enteric helminths can increase or decrease the beneficial bacterial populations in the host, affecting the health of the animal [8]. (fortunepublish.com)

Here we evaluated the viability of amplicon sequencing of the full-length 16S rRNA gene for serotyping S. enterica and E. coli. (bvsalud.org)
This study used 16S rRNA gene sequencing and PICRUSt to assess bacterial community structure and function changes in rhizosphere soil from Meloidogyne incognita -infected tobacco plants. (ppjonline.org)

Antibacterial potential of extracts is explainable on bases of phytochemical composition as ethanolic extract strongly inhibits bacterial growth. (jptcp.com)

One synthetic pathway produced valine at a sufficient level for cell viability and proliferation. (elifesciences.org)

This project aims to develop new carbohydrate-functionalised polymeric surfaces which will inhibit bacterial adhesion. (qub.ac.uk)

It induces directed polymerization of actin by the ActA transmembrane protein, thus pushing the bacterial cell around. (wikipedia.org)
A dose dependent curve was obtained by plotting concentration of dose and cell viability. (jptcp.com)
Together, these results demonstrate that IL-12p70 production in human B cells is under the dual control of microbial stimulation and T cell help. (aai.org)
To determine the safety of the fermented extracts, their cytotoxicity was analyzed by measuring the cell viability of CCD-966SK and human epidermal melanocytes (HEMn) after exposure. (hindawi.com)

Ventilator-associated pneumonia (VAP) is one of the commonest hospital-acquired infections associated with high mortality. (microbe.net)

Bacterial multiplication and the production of soluble proteins called exotoxins require a low oxygen tension. (medscape.com)

The parasite infections of animals (production and pets) affect the health and quality of animal life, the parasites frequently present a microbiome associated which play several functions, like nutrition, reproduction or defense against the host immune system, and in some cases are opportunistic microorganisms. (fortunepublish.com)
It causes opportunistic infections especially in immunocompromised patients. (lookformedical.com)

Typical clinical symptoms of Campylobacter infections in humans are abdominal cramps, diarrhoea and fever. (biomedcentral.com)

Efficacy of certain plant extracts against seed-borne infection of Collectotrichum destructivum on cowpea ( Vigna uniguculata ). (innspub.net)

Then identify a microbial organisms comprising predominantly anaerobic Gram negative for the test. (ndltd.org)

One such factor is the use of antibiotic therapy to treat routine or suspected bacterial infections 1 . (dunianusantara.io)

Des échantillons de muqueuse ont été prélevés grâce à une biopsie antrale sur 136 patients dyspeptiques consécutifs pour lesquels un diagnostic d'ulcère gastro-duodénal avait été établi par endoscopie à l'hôpital général de Bassora (Basra) en Iraq. (who.int)

Such bacterial presence may lead to clinical symptoms of persistent chronic apical periodontitis (CAP), which has high prevalence in some countries, ranging from 40% to 61% 3-4 . (bvsalud.org)

Here, we investigated the potential of Pep19-2.5 and the structurally related compound Pep19-4LF for their therapeutic application in bacterial skin infections. (nature.com)
Conclusively, our data suggest a novel therapeutic target for the treatment of patients with acute and chronic skin infections. (nature.com)

BVCCA has been found superior to chocolate agar for isolation of NTHi from nasopharyngeal swabs. (medscape.com)
Microbial analyses of foods and biological samples are traditionally carried out by direct plating on agar media. (biomedcentral.com)
MCUA showed a higher isolation rate than classic Columbia agar (67.6% versus 44.1% of patients), and the results were obtained faster (24 hours versus 5-7 days) with more clear-cut identification. (who.int)
Om onderdrukking en competitieve interacties tussen enerzijds Listeria monocytogenes en anderzijds enkele goede bacteriën vanop sla te onderzoeken, werd een modelsysteem ontwikkeld in het labo, namelijk sla-agar. (scriptieprijs.be)

Microbial surveillance of the PWS was implemented by NASA to ensure crew health. (jcvi.org)

This ability to survive in distilled water with minimal additives has made them problematic for healthcare, as hospital-acquired BCC infections can arise from contaminated disinfectants, anesthetic solutions, distilled water, and aqueous chlorhexidine solutions. (jcvi.org)
It is the most common healthcare-associated infection in the United States and one of the most common hospital-acquired GI infections around the world 9 . (dunianusantara.io)

Thus, there is need for better strategies to protect existing drugs and develop new classes of effective drugs to treat resistant infections. (biomedcentral.com)

Principal Coordinate Analysis revealed clear separations between bacterial communities from infested and uninfested soil, indicating that different infection conditions resulted in significantly different bacterial community structures in soils. (ppjonline.org)

Broilers have been identified as the major source of campylobacteriosis and reducing Campylobacter loads in the broiler caeca has been proposed as an effective measure to decrease the number of infections in humans. (biomedcentral.com)
Plastic and reconstructive techniques for limb salvage have altered the course of treatment for foot infections, with the goals of functional restoration and a major decrease in amputation rates. (medscape.com)

Test results on body fluids from various sites of infection that reveal small, gram-negative, pleomorphic coccobacilli with polymorphonuclear cells are strong evidence of infection. (medscape.com)

Anatomy4

Organisms6

Chemicals and Drugs13

Analytical, Diagnostic and Therapeutic Techniques and Equipment28

Psychiatry and Psychology1

Phenomena and Processes32

Disciplines and Occupations1

Anthropology, Education, Sociology and Social Phenomena1

Information Science4

Health Care3

Pathogens9

Staphylococcus3

Virulence1

Bacteria13

Antibiotics4

Primary endodontic infections2

Species5

Microbiota2

Detection3

Characterization1

Disinfection4

Fungal infections3

Abstract1

Strains3

Anti-bacterial3

Approaches1

Pathogen2

Enterococcus2

Microbiological1

Biosynthesis2

Urinary tract infe3

Findings2

Biofilms2

Pathogenicity1

Soft-tissue infections2

Genus3

UTIs1

Resistance2

Methods9

Foodborne2

Legionella1

Medical device-related infections1

Specimens3

Assays1

AgNPs1

Parasitic2

Sensitivity1

Escherichia1

Compounds1

Candida1

Colonies1

Viral1

Enteric1

RRNA gene2

Ethanolic extract1

Proliferation1

Adhesion1

Cell4

Pneumonia1

Proteins1

Opportunistic2

Fever1

Efficacy1

Organisms1

Antibiotic1

Diagnostic1

Symptoms1

Therapeutic2

Agar4

Surveillance1

Hospital-acquired2

Resistant1

Significantly1

Decrease2

Gram1