Individual dna probes. Medical search. Frequent questions

DNA microarrays are simply platforms that consist of small solid supports onto which the sequences from thousands of different genes are attached at fixed locations. (medscape.com)
In FISH procedure, fixed tissue sections are pretreated to expose target DNA or mRNA sequences. (biogenex.com)
An appropriately labeled probe is hybridized to the exposed target DNA or mRNA sequences in the cells. (biogenex.com)
First, we collected different individuals of Theodoxus fluviatilis from three different populations to obtain reference DNA sequences and create specific primers and probe for qPCR. (unige.ch)
Spot signal intensities were 18 to 74 times greater for perfect matches than for mismatched target sequences differing by a single nucleotide (discrimination ratio) for "homozygous" DNA from healthy individuals. (hindawi.com)
This is achieved by cleaving genomic DNA into fragments with a restriction enzyme and then introducing common adaptor sequences into the restriction products by ligation. (hindawi.com)
The Molecular Inversion Probe assay (Parallele Biosciences, South San Francisco, Calif, USA) [ 6 ] uses probes that can be circularized and contain two sequences complementary to regions adjacent to SNPs in the DNA target in the first step of the genotyping reaction. (hindawi.com)
All DNA sequences for the three genes known to be involved in CMT disease, for healthy individuals and patients, were confirmed by direct sequencing. (hindawi.com)
Although DNA-based tests are desirable for identifying particular classes of organisms such as pathogens within these complex mixtures, they are limited in the ability to detect specific DNA sequences of targeted organisms. (genomeweb.com)
By using 32P-labeled human growth hormone or the homologous human chorionic somatomammotropin complementary DNA (cDNA) sequences as a probe, the growth hormone genes of affected individuals from all families yielded normal restriction patterns. (jci.org)
The novel method also opens the door for scientists to study the effects of genetic variants on different types of cells and tissues, as the probes can be used to distinguish similar genetic sequences. (nanowerk.com)
The Vysis CEP 12 SpectrumOrange DNA Probe Kit is intended to detect AT rich alpha satellite sequences in the centromere region of chromosome 12 in conjunction with routine diagnostic cytogenetic testing. (molecularcatalog.abbott)
It is possible that some individuals may have target sequences at an alternate chromosomal location that may hybridize with CEP 12. (molecularcatalog.abbott)
DNA) sequences of interest in organisms present in a clinical specimen. (cdc.gov)
In conclution, DNA amplification is a rapid, reliable and accurate method with a high degree of sensitivity and specificity for the detection of Mycobacterium tuberculosis DNA sequences and it can replace the conventional culture method in the diagnosis of extra pulmonary tuberculosis and tuberculosis meningitis except in the situation when antibiotic sensitivity results are required. (who.int)

Each assay consists of a primer pair and a hydrolysis probe with a configurable fluorophore dye. (qiagen.com)
For the dPCR reaction, the assay is combined with the QIAcuity Probe PCR Kit (DNA targets) or the QIAcuity OneStep Advanced Probe Kit (RNA targets). (qiagen.com)
Each assay is based on an endpoint PCR amplification of a species-specific genetic region of the relevant microbe or a region of an individual microbial gene. (qiagen.com)
Data reduction is an issue because Affymetrix GeneChip oligonucleotide microarrays assay each target mRNA with a set of 11 to 16 pairs of 25-nucleotide DNA probes. (biomedcentral.com)
The Western blot assay is a method in which individual proteins of an HIV-1 lysate are separated according to size by polyacrylamide gel electrophoresis. (cdc.gov)
Additional laboratory evaluation included retesting of initial DNA extracts at CDC using a two-target PCR assay (IS 481 and ptxS 1). (cdc.gov)
This assay, described as Amplified hsp65 Restriction Analysis (AHSPRA), can discriminate all the three individual species using a one-step restriction digestion using either BbvI or Eco0109I. (cdc.gov)

Fluorescence in situ hybridization (FISH) is a robust technique of cytogenetic used for the detection of chromosomal aberrations, presence or absence of specific DNA sequence in native context. (biogenex.com)
In ehxA (enterohemolysin encoding) virulence genes by several regions of the world, STEC strains of O group hybridization to individual DNA polynucleotide probes. (cdc.gov)
This review is about "molecular cytogenetics" including 1) the historical perspective of its development from cytogenetics, 2) technical aspects, 3) available probe sets, and 4) variants and applications of the basic fluorescence in situ hybridization (FISH) approach. (frontiersin.org)
After hybridization with perfectly complementary labeled targets, the stems of probes are opened for the subsequent detection of these targets. (hindawi.com)
One of the major limitations of our original method was the use of individual PCR products, amplified separately, for the hybridization and genotyping reaction. (hindawi.com)
Comparative genomic hybridization (CGH) has been developed to elucidate genome-wide sequence copy number variation (CNV) between different genomes, such as the differential amplification or deletion of genetic regions between tumor DNA and normal DNA from neighboring unaffected tissue [ 7-10 ]. (idtdna.com)
High resolution analysis of DNA copy number variation using comparative genomic hybridization to microarrays. (idtdna.com)
Transcribed RNA levels are often measured by hybridization to microarrays of oligonucleotide probes, in which each transcript is represented by multiple probes. (biomedcentral.com)
Specificity of the product was confirmed with hybridization to a labeled probe. (cdc.gov)
The specificity of the product was confirmed by hybridization to a digoxigenin-labeled probe according to the method described in Boehringer Mannhein's Genius System User's Guide for Filter Hybridization. (cdc.gov)

deletions, amplification and translocation in tissue sections or within individual cells. (biogenex.com)
We show here that the multiplex genotyping of multiple mutation sites is possible, based on multiplex PCR DNA amplification and a stem-loop probe method. (hindawi.com)
Analysis at the single-cell level is essential for the understanding of cellular responses in heterogeneous cell populations, but it has been difficult to perform because of the strict requirements put on detection methods with regard to selectivity and sensitivity (i.e., owing to the cross-reactivity of probes and limited signal amplification). (nih.gov)
After a series of successive cycles of amplification, the presence of double stranded DNA product was visualized on agarose gels stained with ethidium bromide. (cdc.gov)
The objective of the study was to develop a rapid method to detect Mycobacterium tuberculosis from clinical samples and the applicability of DNA amplification techniques to a developing country like Sri Lanka. (who.int)

After binding to genomic DNA, these probes are pulled down and washed, allowing exon regions to be selectively sequenced. (digitaljournal.com)
PEs reverse transcribe the pegRNA extension directly into the genome using the nicked target DNA strand as a primer, leading to permanent changes in genomic DNA with relatively few byproducts 2 . (nature.com)
These kits generate high yields of genomic DNA, total cellular DNA or total nucleic acids (TNA) in under 60 minutes. (biosearchtech.com)

3' exonuclease activity of the Taq DNA polymerase cleaves the fluorophore from the probe. (eurogentec.com)
Reliable hot start Taq DNA Polymerase for sensitive detection of low abundance target DNA. (biosearchtech.com)
During PCR testing, Taq polymerase and DNA primers complementary to a unique sequence of target DNA were used to greatly amplify that region if the target was present in the sample. (cdc.gov)
Reverse transcription is the synthesis of a complementary DNA sequence from an RNA template using reverse transcriptase, which is an RNA-dependent DNA polymerase. (medscape.com)
[ 1 ] The resultant complementary DNA is amplified by polymerase chain reaction (PCR). (medscape.com)
In PCR, a thermostable DNA polymerase is used to amplify target DNA 2-fold with each temperature cycle. (medscape.com)
Initially, DNA is taken from the clinical specimen, as well as certain sequence-specific oligonucleotide primers, thermostable DNA polymerase, nucleotides, and buffer. (medscape.com)
Finally, nucleotides complementary to the target DNA are added extending each primer by the thermostable DNA polymerase. (medscape.com)

Building on previous methods developed by the same team, the researchers were able to convert messenger RNA (mRNA) into a type of DNA molecule that can be detected by 'padlock probes', fluorescent probes which lock onto the DNA after hybridisation, making it possible to study DNA repair at the molecular level in individual cells. (nanowerk.com)
Padlock probes can amplify the minority signals making it possible to directly identify genetic variation at the mRNA (messenger ribonucleic acid) level (in molecules produced by active genes) in cells in microscopic preparations. (nanowerk.com)
this is achieved by combining padlock probes with in situ proximity ligation assays (in situ PLA). (nih.gov)
In addition, we provide an example of how to design padlock probes and how to optimize staining conditions for fixed cells and tissue sections. (nih.gov)
Both padlock probes and in situ PLA provide the ability to directly visualize single molecules by standard microscopy in fixed cells or tissue sections, and these methods may thus be valuable for both research and diagnostic purposes. (nih.gov)

Furthermore, LNA® offers the possibility to adjust Tm values of primers and probes in multiplex assays. (eurogentec.com)
Finally, the pooling of DNA samples or multiplex PCR strategies for SNP genotyping have also been described in several microarray methods, such as the original arrayed primer extension (APEX) [ 9 - 12 ] or single base extension (SBE-tags) [ 13 , 14 ] assays. (hindawi.com)
Contrast this current state of DNA-based or molecular diagnostic assays with the GEN article written in 1983, which appears below. (genengnews.com)
dPCR Microbial DNA Detection Assays are intended for molecular biology applications. (qiagen.com)
The dPCR Microbial DNA Detection Assays and the QIAcuity dPCR System enable accurate digital PCR-based quantification. (qiagen.com)
Our dPCR Microbial DNA Detection Assays can be combined for multiplex analysis. (qiagen.com)
A comparison between singleplex and multiplex analysis using the dPCR Microbial DNA Detection Assays shows similar and highly precise quantification for all targets (see figure Microbial detection in multiplex dPCR on QIAcuity ). (qiagen.com)
The dPCR Microbial DNA Detection Assays are intended for use in nanoplate digital PCR. (qiagen.com)
Heat-labile Cod Uracil-DNA Glycosylase prevents carry-over contamination in PCR, qPCR, RT-qPCR, and RT-LAMP assays. (sigmaaldrich.com)
This greatly enhanced the sensitivity of assays used to detect that specific sequence of DNA. (cdc.gov)

The project will begin by evaluating different primers and probes on known bacterial strains with the goal of creating a multiplex PCR where all three bacteria are included in the same real-time PCR. (lu.se)

The 2 most common microarray technologies in use are the oligonucleotide microarrays and the robotically spotted complementary DNA (cDNA) microarrays. (medscape.com)
Eurogentec is a supplier of Double-Dye Oligonucleotide probes and offers the largest number of fluorescent reporters and quenchers. (eurogentec.com)
Double-Dye Oligonucleotide probes with various non-fluorescent quenchers and a variety of fluorescent dyes can be conveniently ordered using our web ordering interface. (eurogentec.com)
The optimal recommended length of Double-Dye Oligonucleotide probes is 25 bases. (eurogentec.com)
The Tm of the Double-Dye Oligonucleotide probe must be higher than the upstream primer, to avoid that the later primer is already being extended while the Double-Dye Oligonucleotide probe is not yet annealed. (eurogentec.com)

Recent advances in recombinant DNA technology, as well as focused research efforts to characterize the genetic defects involved in the transmission of inherited diseases, promise to make earlier diagnosis and disease prevention a reality. (genengnews.com)
The basic principle of the mapping scheme was to develop, by recombinant DNA techniques, random single-copy DNA probes capable of detecting DNA sequence polymorphisms when hybridized to restriction digests, or specific fragments, of an individual's DNA. (nih.gov)
The use of recombinant inbred lines allows an estimate of the heritability of expression measured by individual probes. (biomedcentral.com)
In addition, recombinant inbred lines can provide both replicates from genetically identical individuals and samples from different segregants. (biomedcentral.com)

The introduction of complementary DNA (cDNA) microarray technology in 1995 has helped to facilitate the identification and classification of DNA sequence information and the assignment of functions to these new genes by allowing investigators to analyze expression of thousands of genes simultaneously in a single experiment. (medscape.com)
In this technique florescent probes bind to the target sequence of DNA in chromosome. (biogenex.com)
In-solution capture utilizes pools of oligonucleotides or probes bound to magnetic beads, whose sequence has been designed to hybridize to exon regions. (digitaljournal.com)
Each pair of probes consists of a perfect match (PM) sequence and a mismatch (MM) sequence, the latter intended to estimate nonspecific binding. (biomedcentral.com)
RMA provides statistically robust averaging methods, dChip fits a model that allows probe-specific binding affinities, and PDNN fits a model that allows sequence-specific binding affinities and nearest-neighbor stacking interactions. (biomedcentral.com)

In the 1990s Botstein, having moved to Stanford University School of Medicine, collaborated with Patrick O. Brown of Stanford in exploiting DNA microarrays to study genome-wide gene expression patterns in yeast and in human cancers. (nih.gov)

The recent discovery that DNA methylation survives in fossil material provides an opportunity for novel molecular approaches in palaeogenomics. (ku.dk)
While exome capture methods using PCR, hybrid capture and molecular inversion probes exist, the most common and efficient strategies are in-solution capture methods. (digitaljournal.com)

Messenger RNA (mRNA) from the sample of interest can serve as a template for producing complementary DNA (cDNA) in the presence of a reverse transcriptase enzyme. (medscape.com)

Furthermore, as many applications, like genotyping or gene expression profiling, are requiring small amounts of several different probes, Eurogentec has developed a high-throughput platform to produce high quality small scale Real-Time qPCR Double-Dye probes. (eurogentec.com)

Double-Dye Oligonucleotides are provided lyophilized in individual 2 mL tubes. (eurogentec.com)
In case of extra long Double-Dye Oligonucleotides probes are required, quenchers like Black Hole Quencher®, Deep Dark Quencher I and TAMRA can be coupled internally. (eurogentec.com)

Her project involves synthesising fluorescent probes to visualise G-quadruplexes in live cells and establishing correlations between their presence and epigenetic characteristics in cancer. (imperial.ac.uk)

Her project aims to use high-throughput synthesis and testing to develop optical probes for G-quadruplex DNA. (imperial.ac.uk)
Our mag kits are a high-throughput and cost-effective DNA isolation method suitable for PCR, sequencing and genotyping. (biosearchtech.com)

Genotoxic chemicals interfere with the normal process of DNA (deoxyribonucleic acid) repair, and can affect cancer risk in individuals. (nanowerk.com)
DNA (deoxyribonucleic acid) is the genetic material present in every cell of the human body. (findlaw.com)

The presence of more than 300 bacterial species is analyzed using 54 different DNA probes. (his.se)
A new, kiwi specific DNA probe was trialled and the remaining stomach contents were sequenced for other native wildlife species. (helsinki.fi)
The DNA sequencing however revealed five other species: tomtit (lat. (helsinki.fi)
Here, we examine the occurrence of this species in the Upper Rhone river by morphological observations and environmental DNA detection, in order to assess the impact of environmental changes on this particularly sensitive ecosystem. (unige.ch)
Environmental DNA (eDNA) detection is a non‐invasive technique that can provide reliable information about a macrobial species occurrence based on intra- and extracellular material shed by an organism into the environment. (unige.ch)
Microorganisms in the environment are typically in complex mixtures of hundreds or more individual species. (genomeweb.com)
Using remains of a Palaeo-Eskimo Saqqaq individual, woolly mammoths, polar bears and two equine species, we confirm that DNA methylation survives in a variety of tissues, environmental contexts and over a large temporal range (4,000 to over 45,000 years before present). (ku.dk)
Although the pathogens in the above cultures may look alike, DNA data indicate they represent distinctly different species within the Fusarium graminearum complex. (usda.gov)
Ward and O'Donnell envision using the test to understand the distribution of these pathogens worldwide, as well as to determine if individual pathogen species prefer certain crops or environments. (usda.gov)
How do we make the connections from individual species fitness to population growth rates? (explorers.org)
DNA probes are available for several species. (medscape.com)

DNA was extracted from blood samples with the Nucleon extraction and purification kit (RPN8512, Amersham Biosciences, GE Healthcare AB, Uppsala, Sweden), used according to the manufacturer's instructions. (hindawi.com)
For the DNA extraction and purification from the clinical samples two methods, the standard phenol extraction procedure desctibed by Sambrook etal (1982) and the guanidinium thiocyanate method described by Boom et al(1990) were used. (who.int)

His project aims at developing molecules that target different DNA and RNA topologies. (imperial.ac.uk)
Here, we apply to ancient DNA extracts the probe-independent Methylated Binding Domains (MBD)-based enrichment method, which targets DNA molecules containing methylated CpGs. (ku.dk)
The concept of localizing individual molecules with high precision was first described by the German physicist Werner Heisenberg in the 1930s and was more formally addressed with a strong mathematical foundation during the 1980s - 1990s by several research groups. (microscopyu.com)
Usually, the NOE and NMRD in B-DNA is expected to be less ``integral'' than methods can only provide bounds on the residence water molecules buried inside proteins. (lu.se)

Other "in-house" PCRs have been developed at individual laboratories to diagnose infections (eg, testing of cerebrospinal fluid [CSF] for herpes simplex virus to diagnose herpes encephalitis and testing of nasopharyngeal wash fluid to diagnose Bordetella pertussis infection ), particularly if traditional culture and antigen detection techniques have failed. (medscape.com)

The individual DNA strands are called probes. (medscape.com)
The temperature of these is increased to 90-95°C in order to separate (denature) the 2 strands of target DNA. (medscape.com)

The DNA of each patient bores one heterozygous mutation in one of these genes. (hindawi.com)
Data from these define genetic and non-genetic variation, define a measure of heritability for expression of individual genes, and provide the basis for a new method of data reduction for genetical genomics. (biomedcentral.com)

His project aims at developing novel proximity-labeling methods for mapping the protein interactome of G4 DNA using photochemical processes. (imperial.ac.uk)
Several alternative methods have been proposed to combine multiple probe-specific values into a single expression estimate. (biomedcentral.com)

Except for identical multiples (such as twins or triplets), each individual's DNA is unique. (findlaw.com)

Polymorphic restriction endonuclease sites (HincII and MspI), which are closely linked to the structural gene for growth hormone on chromosome 17, were used as markers in linkage analysis of DNA of family members. (jci.org)
Onadim, Z, Hungerford, J & Cowell, JK 1992, ' Follow-up of retinoblastoma patients having prenatal and perinatal predictions for mutant gene carrier status using intragenic polymorphic probes from the rbi gene ', British Journal of Cancer , vol. 65, no. 5, pp. 711-716. (elsevierpure.com)

This system now combines the principle of highly specific genotyping based on stem-loop structure probes with the advantages of multiplex analysis. (hindawi.com)

All probes are HPLC purified. (biosearchtech.com)

Results obtained from Restriction fragment length polymorphism typing show that the mahority of circulating Mycobacterium tuberculosis strains in Sri Lanka belong to a limited number of families, but the degree of IS6160 DNA polymorphism among strains were high. (who.int)

Heterozygous" mutant DNA samples gave signal intensity ratios close to 1 at the positions of the mutations as expected. (hindawi.com)
Over 1,000 diseases are now known to be transmitted via defects or mutations in the genetic material, DNA. (genengnews.com)

Optical indicators provide a powerful means to reveal such distributed activity, particularly when protein-based and encodable in DNA: encodable probes can be introduced into cells, tissues, or transgenic organisms by genetic manipulation, selectively expressed in anatomically or functionally defined groups of cells, and, ideally, recorded in situ, without a requirement for exogenous cofactors. (nih.gov)
Nanowerk News ) EU-funded scientists in Sweden have developed a novel method to study genetic variation directly in individual cells and in tissues. (nanowerk.com)

No suspect was identified, and allegedly no fingerprints, DNA samples, or leads were found! (thegatewaypundit.com)
It appears the DNA samples were not only found but also preserved in an evidence vault. (thegatewaypundit.com)
Primetime ask the Secret Service, why didn't you take DNA samples from your suspect list? (thegatewaypundit.com)
The composition of the bacterial flora in the intestine is determined by sequencing bacterial DNA from stool samples. (his.se)
This method, thus, appears only appropriate for the analysis of ancient methylomes from very well preserved samples, where both DNA fragmentation and deamination have been limited. (ku.dk)
Contamination of PCR samples with amplicon DNA generated by previous reactions, "carry-over contamination", is one of the major sources of false positive results. (sigmaaldrich.com)
In four cases foetal DNA samples were obtained by chorionic villus sampling, the remaining six were derived from either cord blood samples or venipuncture of neonates. (elsevierpure.com)
Samples were tested for the presence of amplifiable DNA and absence of inhibitors by performing beta globin PCR. (cdc.gov)
450 individuals and 5000 samples in The Gambia as well as for analyzes of a smaller study of 176 individuals with throat infection that were not sampled longitudinally but collected by the same study group (12). (lu.se)

Using the 500,000-probe array, users can detect the presence of up to 9,000 bacterial agents, according to a statement from LBNL. (genomeweb.com)

DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. (idtdna.com)
In all cases standard linkage studies were employed using intragenic DNA probes which recognise restriction fragment length polymorphisms. (elsevierpure.com)

Nuclear DNA from individuals belonging to nine different families in which two sibs were affected with isolated growth hormone deficiency type I were studied by restriction endonuclease analysis. (jci.org)
1. Feinberg AP, Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity . (idtdna.com)

The sensitivity of the PCR method is largely dependent on the efficiency of the DNA extraction procedure which, could probably be improved by modifications of the lysis technique. (who.int)

contained 0.5 µL of each primer (10 with serology suggestive of past HBV pmol/µL) and the probe (SP2, 10 infection and clearance of HBsAg [1,2]. (who.int)

At first, the digital certificate will be used to verify that each individual has surrendered their body to unreliable covid-19 nasal probes or DNA swabs. (naturalnews.com)
Common Pass requires each traveler to submit to DNA swabs, lab testing and vaccination. (naturalnews.com)
During summer 2018-2019, three out of eight monitored kiwi chicks were predated by a suspected mustelid/mustelids and DNA swabs were obtained from the bite sites. (helsinki.fi)

The use of karyotyping to determine genetic defects is highly accurate for determining chromosomal abnormalities in individuals and prenatal fetuses. (genengnews.com)

In the diet part of this thesis, we trialled the kiwi specific probe but could not identify any kiwi DNA in the stoat stomach contents. (helsinki.fi)
We previously developed a highly specific method for detecting SNPs with a microarray-based system using stem-loop probes. (hindawi.com)
We previously developed a highly specific genotyping method based on hairpin probes. (hindawi.com)
Our probes, also called hairloops, consist of a GC-rich stem and a target-specific loop [ 15 ]. (hindawi.com)
Eventually, scientists hope to pinpoint the exact DNA defects causing disease, and develop more specific, simpler tests. (genengnews.com)
The rationale for this method is that MM probes exhibit probe-specific binding as well as nonspecific binding [ 15 , 17 ] and may therefore be more effective for estimating specific binding than for correcting for nonspecific binding. (biomedcentral.com)
Specific DNA present in small amounts in a clinical specimen are amplified by PCR so they become detectable. (medscape.com)
The amplified segment may be observed using electrophoretic gel or can be identified by Southern blot analysis, using specific DNA probes for that segment. (medscape.com)

Long-range PCR for amplifying long PCR fragments, up to 10 kb in length, in each individual PCR has also been described [ 4 , 5 ]. (hindawi.com)
Inactivation of CIP2A-TOPBP1 caused acentric fragments to disperse throughout the mitotic cytoplasm, stochastically partition into the nucleus of both daughter cells and aberrantly misaccumulate as cytoplasmic DNA. (nature.com)
Mitotic clustering facilitates the reassembly of acentric fragments into rearranged chromosomes lacking the extensive DNA copy-number losses that are characteristic of canonical chromothripsis. (nature.com)
The stochastic inheritance of chromosome fragments by both newly formed daughter cells could in part contribute to the alternating DNA copy-number states that are characteristic of chromothripsis 2 . (nature.com)

In clinical practice, the WHO-enforced six-step method has proven to be a valuable tool to systematically choose the best pharmacotherapy available and the most appropriate dose for each individual patient. (springer.com)
Surveillance case definitions are not intended to be used by healthcare providers for making a clinical diagnosis or determining how to meet an individual patient's health needs. (cdc.gov)
but with incomplete penetrance (clinical symptoms are not always present in individuals who have the disease-causing mutation). (msdmanuals.com)

The latter contains the methodology itself, the types of probe- and target-DNA, as well as probe sets. (frontiersin.org)
This fluorescence can be measured, and the level is directly proportional to the amount of target DNA accumulating during the PCR reaction. (eurogentec.com)
Prime editors (PEs) are precision gene editing agents that can perform virtually any substitution, small deletion and small insertion at target DNA sites in living cells 2 . (nature.com)
A prime editing guide RNA (pegRNA) contains a spacer that guides the PE to the target DNA site as well as a 3′ extension that encodes the desired edit. (nature.com)
In the second step, the temperature decreases (45-60°C), depending on the primers, to permit annealing (strengthening) of the target DNA primers. (medscape.com)
The target DNA segment is amplified in the range of 10 5 - to 10 6 -fold by repeating this cycle no less than 30-40 times. (medscape.com)
Contamination of reagents or specimens with target DNA from the environment must be avoided because such contamination can produce false-positive results. (medscape.com)

Compared to DNA Double-Dye probes, LNA® Double-Dye probes exhibit higher thermal stabilities, specificity and reproducibility. (eurogentec.com)

It is not intended for use with test matrices other than peripheral blood lymphocytes from subjects with CLL, to screen for chromosome 12 aneuploidy, eg, in asymptomatic individuals, or to monitor patients for residual disease. (molecularcatalog.abbott)
The CEP 12 DNA Probe Kit has been optimized only for identifying chromosome 12 in interphase nuclei from peripheral blood specimens from patients with B-cell chronic lymphocytic leukemia. (molecularcatalog.abbott)

HBV-DNA was detectable in sera from 3 patients out of the 40 patients who were positive for hepatitis B core antibody. (who.int)

DNA within a culture-negative cerebrospinal fluid (CSF) specimen. (cdc.gov)

Like sbeadex, mag uses magnetic beads to bind DNA but uses salt and alcohol-based wash buffers to remove contaminants before the final elution step. (biosearchtech.com)

The main part deals with examples of modern FISH-applications, highlighting unique possibilities of the approach, like the possibility to study individual cells and even individual chromosomes. (frontiersin.org)

The researchers looked at areas of the genome with tandem repeats - stretches of 2 to 20 nucleotides, which are the 'building blocks' of DNA, that are repeated two or more times in one spot. (spectrumnews.org)

For example, early reports of the imaging of DNA in scanning tunneling microscopes (STMs) used highly ordered pyrolitic graphite (HOPG) substrate, and subsequent investigations showed that at least some of the features reported were not those of DNA at all but of artifacts on the HOPG surface. (nationalacademies.org)
Subsequent stringent washing steps remove any probe that is non-specifically bound to the tissue section. (biogenex.com)

Mechanistically, the CIP2A-TOPBP1 complex prematurely associates with DNA lesions within ruptured micronuclei during interphase, which poises pulverized chromosomes for clustering upon mitotic entry. (nature.com)

Quantitative changes in DNA can indicate whether a child is trisomic or afflicted with Down's syndrome. (genengnews.com)

The method allows us to study biological processes in individual cells, as opposed to the average states of large numbers of cells,' explained Dr Nilsson. (nanowerk.com)
DNA solution, however, are in a range where the ecules in the minor groove of DNA are exposed to residence time can actually be pinpointed by either the external solvent medium and should therefore method. (lu.se)
Direct cell-lysis was used instead of the conventional DNA template preparation, which improved the rapidity, simplicity and adaptability of the developed method. (cdc.gov)

the CEP 12 DNA Probe Kit has been optimized for archived peripheral blood specimens stored only at - 20°C or - 80°C, as recommended. (molecularcatalog.abbott)

In neural systems, information is often carried by ensembles of cells rather than by individual units. (nih.gov)
Simple, rapid extraction of PCR-ready DNA for screening and genotyping type applications from hair follicles, quill-end cells of feathers, tissue-culture cells, buccal cells and other sample types. (biosearchtech.com)

When linked to a vesicle membrane protein, pHluorins were sorted to secretory and synaptic vesicles and reported transmission at individual synaptic boutons, as well as secretion and fusion pore 'flicker' of single secretory granules. (nih.gov)
Genomics has resulted in a parallel consensus that the central functions of all eukaryotes are highly conserved, not only at the level of individual protein functions, but also complex biological pathways and systems. (nih.gov)

It is also more affordable enabling the analysis of more individuals and populations. (digitaljournal.com)
Comprehensive analysis of pan-cancer genomes revealed clusters of DNA copy-number-neutral rearrangements-termed balanced chromothripsis-across diverse tumour types resulting in the acquisition of known cancer driver events. (nature.com)

Anatomy11

Organisms12

Diseases9

Chemicals and Drugs48

Analytical, Diagnostic and Therapeutic Techniques and Equipment32

Phenomena and Processes38

Disciplines and Occupations4

Anthropology, Education, Sociology and Social Phenomena1

Technology, Industry, Agriculture4

Humanities1

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Health Care4

Sequences15

Assay7

Hybridization10

Amplification5

Genomic3

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Padlock probes5

Assays10

Primers and probes1

Oligonucleotide5

Recombinant4

Sequence5

Microarrays1

Molecular2

Complementary1

QPCR1

Oligonucleotides2

Fluorescent probes1

High-throughput2

Deoxyribonucleic acid2

Species11

Extraction and purification2

Molecules4

Detection1

Strands2

Genes2

Methods2

Individual's1

Polymorphic2

Multiplex analysis1

HPLC1

Strains1

Mutations2

Tissues2

Samples9

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Polymorphisms2

Restriction endonuclease2

Sensitivity1

Primer1

Swabs3

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Specific8

Fragments4

Clinical3

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Analysis2