Testing of serum at 1:200 dilution is proposed for epidemiological screening. (edu.in)
Results2
The test was based on the detection of IgG antibodies to Pseudomonas mallei antigens bound to nitrocellulose coated on plastic strips (dipsticks), the reaction being amplified by an avidinbiotin system with biotinylated anti-horse IgG and horseradish peroxidase-avidin D. Sera from 810 normal, six naturally infected and 48 sensitized equines were tested by this assay and results were compared with complement fixation, indirect haemagglutination and counter-immunoelectrophoresis tests. (edu.in)
Dot ELISA had the highest sensitivity and was superior to other tests in that it was rapid and easy to perform, the results were easy to interpret, the assay was not influenced by anti-completement activity and it was able to detect antibodies at an early stage. (edu.in)
ELISA5
82% of 2434 schoolchildren (aged 7-12 years) identified in a census were screened for antibodies to T cruzi by indirect immunofluorescence, indirect haemagglutination, and ELISA. (nih.gov)
Original investigations were eligible if they estimated sensitivity and specificity, or reliability -or if their calculation was possible - of ELISA or PCR tests, for chronic Chagas disease. (biomedcentral.com)
Heterogeneity was high within each test (ELISA and PCR) and threshold effect was detected only in a particular subgroup. (biomedcentral.com)
Both conventional and recombinant based ELISA give useful information, however there are commercial tests without technical reports and therefore were not included in this review. (biomedcentral.com)
Enzyme-Linked ImmunoSorbent Assays (ELISA) are the most sensitive serological tests. (biologyease.com)
Serological tests7
The secondary endpoint was the reduction of antibody titres on repeated serological tests. (nih.gov)
Most current guidelines recommend two serological tests to diagnose chronic Chagas disease. (biomedcentral.com)
When serological tests are persistently inconclusive, some guidelines recommend molecular tests. (biomedcentral.com)
Physicians need to have access to technical reports to understand if these serological tests are similar to those included in this review and therefore correctly order and interpret test results. (biomedcentral.com)
Thus, the diagnosis relies almost solely on serological tests. (biomedcentral.com)
For diagnostic immunological tests, the serological tests must possess high specificity and sensitivity. (biologyease.com)
Precipitation reactions can be easily observed in vitro, therefore they play an important role in serological tests. (biologyease.com)
Antibody4
Application of indirect hemagglutination test and indirect fluorescent antibody test for IgM antibody for diagnosis of melioidosis in Thailand. (nih.gov)
If in a diagnostic test an antibody is capable of detecting a single antigen molecule, then such a test possesses the highest sensitivity. (biologyease.com)
The amount of antigen detected in a test is directly proportional to the amount of antibody used. (biologyease.com)
IHAT negative results were obtained from tests of 50 normal blood donors and 50 sewerage workers. (nih.gov)
Titres2
The IHAT showed good specificity since negative titres were seen in tests using sera from 2 patients with culture-positive Pseudomonas aeruginosa and 4 patients positive for Legionella. (nih.gov)
Of 683 national servicemen tested, 5 (0.73%) had IHAT titres ranging from 1:16 to 1:128. (nih.gov)
Diagnosis1
Currently, PCR should not be used in clinical practice for chronic Chagas disease diagnosis and there is no PCR test commercially available for this purpose. (biomedcentral.com)
Positive2
Using the polysaccharide (melioidin)-sensitized turkey red cells in the indirect haemagglutination test (IHAT), 20 (100%) of the Pseudomonas pseudomallei culture-positive cases were detectable by the IHAT with titles ranging from 1:16 to 1:32, 768. (nih.gov)
130 were positive in all tests and were randomly assigned benznidazole (7.5 mg/kg daily for 60 days by mouth) or placebo. (nih.gov)