• substance
  • This invention presents novel assay methods employing a capture reagent, involving a first binding member typically conjugated to a polymeric anion substance, and a solid phase material containing a reaction site comprising a polymeric cation substance having a nitrogen content of at least about two. (google.ca)
  • The use of a polycationic substance, having a nitrogen content of at least about two percent (excluding the counter ions), to prepare a suitably charged solid phase provides a solid phase that can be subjected to a greater extent of manipulation, such as multiple washes, without losing the capability to attract and retain the capture reagent. (google.ca)
  • wherein
  • 11. The method of claim 9, wherein the enzyme catalyzes the conversion of a non-chemiluminescent reagent into a chemiluminescent product. (freepatentsonline.com)
  • 6. The method according to Claim 1 , further comprising the step of combining the test sample and said capture reagent to form a reaction mixture, wherein said reaction mixture is contacted to said solid phase. (google.ca)
  • 7. The method according to Claim 1 , further comprising the addition of an ancillary specific binding member, wherein said ancillary specific binding member is capable of binding the analyte and is capable of binding a member selected from the group consisting of said indicator reagent or said capture reagent in an indirect assay. (google.ca)
  • react
  • RMg+ RMg+ + X− → RMgX A limitation of Grignard reagents is that they do not readily react with alkyl halides via an SN2 mechanism. (wikipedia.org)
  • Drops
  • Drops of safe reagent are then placed into the wells in sequence, which first bind with the cholesterol in the skin and then subsequently change color in proportion to the amount of bound cholesterol present. (issuu.com)
  • After colonies have grown on the medium, 2-3 drops of the reagent DMPD are added to the surface of each organism to be tested. (wikipedia.org)
  • reactions
  • While pH indicators work efficiently at their designated pH range, they are usually destroyed at the extreme ends of the pH scale due to undesired side reactions. (wikipedia.org)
  • Grignard reactions and reagents were discovered by and are named after the French chemist François Auguste Victor Grignard (University of Nancy, France), who published it in 1900 and was awarded the 1912 Nobel Prize in Chemistry for this work. (wikipedia.org)
  • Similar pathways are assumed for other reactions of Grignard reagents, e.g., in the formation of carbon-phosphorus, carbon-tin, carbon-silicon, carbon-boron and other carbon-heteroatom bonds. (wikipedia.org)
  • On the other hand, they readily participate in transmetalation reactions: RMgX + AlX → AlR + MgX2 For this purpose, commercially available Grignard reagents are especially useful because this route avoids the problem with initiation. (wikipedia.org)
  • In reactions involving Grignard reagents, it is important to exclude water and air, which rapidly destroy the reagent by protonolysis or oxidation. (wikipedia.org)
  • form
  • In one embodiment a test sample suspected of containing the analyte of interest may be contacted with the capture reagent to form a charged capture reagent/analyte complex. (google.ca)
  • The invention preferably relates to a process for the production of test strips in which the reagent zone and the test strip holder form a plane, planar carrier film. (google.com)
  • The general reaction scheme of a pH indicator can be formulated as: HInd + H 2O ⇌ H 3O+ + Ind− Here, HInd stands for the acid form and Ind− for the conjugate base of the indicator. (wikipedia.org)
  • Grignard reagents are similar to organolithium reagents because both are strong nucleophiles that can form new carbon-carbon bonds. (wikipedia.org)
  • The Ehrlich reagent works by binding to the C2 position of two indole moieties to form a resonance stabilised carbenium ion compound. (wikipedia.org)
  • the hydride reagent reacts with water to form solid calcium hydroxide, while it is also a strong enough base to generate the trityl anion. (wikipedia.org)
  • Temperature
  • The transition range may shift slightly depending on the concentration of the indicator in the solution and on the temperature at which it is used. (wikipedia.org)
  • change
  • The hydrophilic group on the ammonia salts also change the dissociation of the reagent to be used with bromopyrogallol red to a more acidic region. (wikipedia.org)
  • Normally, the indicator causes the color of the solution to change depending on the pH. (wikipedia.org)
  • for example, olfactory indicators show change in their odor. (wikipedia.org)
  • For example, when carbohydrates are fermented, the pH within the well decreases and that change is indicated by a change in the color of the pH indicator. (wikipedia.org)
  • uses
  • The "improved hallucinogen reagent", which uses 5% DMAB in 1:1 concentrated phosphoric acid (specific gravity 1.75) / methanol mixture. (wikipedia.org)
  • addition
  • The addition of the Grignard reagent to the carbonyl typically proceeds through a six-membered ring transition state. (wikipedia.org)
  • During incubation, metabolism produces color changes that are either spontaneous or revealed by the addition of reagents. (wikipedia.org)
  • give
  • The reagent will also give a positive result for opium, despite the opiates not containing the indole functional group, because of the presence of tryptophan in natural opium. (wikipedia.org)
  • simple
  • The principle can be illustrated by taking the indicator to be a simple acid, HA, which dissociates into H+ and A−. HA ⇌ H+ + A− The value of the acid dissociation constant, pKa, must be known. (wikipedia.org)
  • solid
  • The complex is then contacted to the oppositely charged solid phase to attract, attach, and immobilize the capture reagent/analyte complex. (google.ca)
  • group
  • The Grignard reagent functions as a nucleophile, attacking the electrophilic carbon atom that is present within the polar bond of a carbonyl group. (wikipedia.org)
  • blue
  • By reacting the bromopyrogallol red with niobium(V) in a tartrate medium at pH 6.0, an intense blue coloured 3:1 reagent was formed. (wikipedia.org)
  • The reagent is a dark-blue to maroon color when oxidized, and colorless when reduced. (wikipedia.org)
  • common
  • Process for the production of a test devices using extruder casting machines or cascade casting machines is disclosed in which reagent zones are produced on a common surface with remaining portion of test device such that reagent zone is protected by being in the same plane as a portion of the test device. (google.com)
  • Common oxidants used in this process are permanganate (both sodium permanganate and potassium permanganate), Fenton's Reagent, persulfate, and ozone. (wikipedia.org)
  • System
  • With the HRP-H2O2-BPR catalytic system, the bromopyrogallol red is determined to be an efficient reagent and quantitation of HRP can be performed. (wikipedia.org)
  • present
  • pH indicators that are weak protolytes, the Henderson-Hasselbalch equation for them can be written as: pH = pKa + log10 [Ind− ] / [HInd] The equation, derived from the acidity constant, states that when pH equals the pKa value of the indicator, both species are present in a 1:1 ratio. (wikipedia.org)
  • Fenton's reagent was initially used to treat hydrocarbon sites where benzene, toluene, and ethylbenzene were present. (wikipedia.org)