• assay
  • This invention presents novel assay methods employing a capture reagent, involving a first binding member typically conjugated to a polymeric anion substance, and a solid phase material containing a reaction site comprising a polymeric cation substance having a nitrogen content of at least about two. (google.ca)
  • 7. The method according to Claim 1 , further comprising the addition of an ancillary specific binding member, wherein said ancillary specific binding member is capable of binding the analyte and is capable of binding a member selected from the group consisting of said indicator reagent or said capture reagent in an indirect assay. (google.ca)
  • The disclosure relates to a dry reagent assay device having two or more test zones for detecting two or more analytes in a test sample. (google.com)
  • Interchim manufactures protein assays (BC Assay, Coo Assay), superior fluorescent dyes known as FluoProbes, Cell Assays reagents and kits (i.e. (wikipedia.org)
  • FluoProbes indicators, high sensitive viability assay UptiBlue) and an innovate transfection agent UptiFectin. (wikipedia.org)
  • substance
  • The use of a polycationic substance, having a nitrogen content of at least about two percent (excluding the counter ions), to prepare a suitably charged solid phase provides a solid phase that can be subjected to a greater extent of manipulation, such as multiple washes, without losing the capability to attract and retain the capture reagent. (google.ca)
  • react
  • RMg+ RMg+ + X− → RMgX A limitation of Grignard reagents is that they do not readily react with alkyl halides via an SN2 mechanism. (wikipedia.org)
  • Drops
  • Drops of safe reagent are then placed into the wells in sequence, which first bind with the cholesterol in the skin and then subsequently change color in proportion to the amount of bound cholesterol present. (issuu.com)
  • After colonies have grown on the medium, 2-3 drops of the reagent DMPD are added to the surface of each organism to be tested. (wikipedia.org)
  • reactions
  • While pH indicators work efficiently at their designated pH range, they are usually destroyed at the extreme ends of the pH scale due to undesired side reactions. (wikipedia.org)
  • Grignard reactions and reagents were discovered by and are named after the French chemist François Auguste Victor Grignard (University of Nancy, France), who published it in 1900 and was awarded the 1912 Nobel Prize in Chemistry for this work. (wikipedia.org)
  • Similar pathways are assumed for other reactions of Grignard reagents, e.g., in the formation of carbon-phosphorus, carbon-tin, carbon-silicon, carbon-boron and other carbon-heteroatom bonds. (wikipedia.org)
  • On the other hand, they readily participate in transmetalation reactions: RMgX + AlX → AlR + MgX2 For this purpose, commercially available Grignard reagents are especially useful because this route avoids the problem with initiation. (wikipedia.org)
  • In reactions involving Grignard reagents, it is important to exclude water and air, which rapidly destroy the reagent by protonolysis or oxidation. (wikipedia.org)
  • form
  • The invention preferably relates to a process for the production of test strips in which the reagent zone and the test strip holder form a plane, planar carrier film. (google.com)
  • Grignard reagents are similar to organolithium reagents because both are strong nucleophiles that can form new carbon-carbon bonds. (wikipedia.org)
  • The Ehrlich reagent works by binding to the C2 position of two indole moieties to form a resonance stabilised carbenium ion compound. (wikipedia.org)
  • the hydride reagent reacts with water to form solid calcium hydroxide, while it is also a strong enough base to generate the trityl anion. (wikipedia.org)
  • zones
  • Process for the production of a test devices using extruder casting machines or cascade casting machines is disclosed in which reagent zones are produced on a common surface with remaining portion of test device such that reagent zone is protected by being in the same plane as a portion of the test device. (google.com)
  • hydrogen
  • Fenton's reagent (hydrogen peroxide catalyzed with iron) and potassium permanganate are the oxidants that have been used the longest, and are now used the most widely. (wikipedia.org)
  • laboratory
  • Tabulated below are several common laboratory pH indicators. (wikipedia.org)
  • Interchim is a privately owned French company specialized in manufacturing and distribution of reagents, consumables and dedicated instruments for the R&D and industry laboratory in the fields of fine chemistry, chromatography and bio-analysis. (wikipedia.org)
  • change
  • The hydrophilic group on the ammonia salts also change the dissociation of the reagent to be used with bromopyrogallol red to a more acidic region. (wikipedia.org)
  • Normally, the indicator causes the color of the solution to change depending on the pH. (wikipedia.org)
  • for example, olfactory indicators show change in their odor. (wikipedia.org)
  • For example, when carbohydrates are fermented, the pH within the well decreases and that change is indicated by a change in the color of the pH indicator. (wikipedia.org)
  • Range
  • This pH range varies between indicators, but as a rule of thumb, it falls between the pKa value plus or minus one. (wikipedia.org)
  • Indicators usually exhibit intermediate colors at pH values inside the listed transition range. (wikipedia.org)
  • The transition range may shift slightly depending on the concentration of the indicator in the solution and on the temperature at which it is used. (wikipedia.org)
  • The figure on the right shows indicators with their operation range and color changes. (wikipedia.org)
  • uses
  • The "improved hallucinogen reagent", which uses 5% DMAB in 1:1 concentrated phosphoric acid (specific gravity 1.75) / methanol mixture. (wikipedia.org)
  • addition
  • The addition of the Grignard reagent to the carbonyl typically proceeds through a six-membered ring transition state. (wikipedia.org)
  • During incubation, metabolism produces color changes that are either spontaneous or revealed by the addition of reagents. (wikipedia.org)
  • presence
  • The reagent will also give a positive result for opium, despite the opiates not containing the indole functional group, because of the presence of tryptophan in natural opium. (wikipedia.org)
  • simple
  • The principle can be illustrated by taking the indicator to be a simple acid, HA, which dissociates into H+ and A−. HA ⇌ H+ + A− The value of the acid dissociation constant, pKa, must be known. (wikipedia.org)
  • group
  • The Grignard reagent functions as a nucleophile, attacking the electrophilic carbon atom that is present within the polar bond of a carbonyl group. (wikipedia.org)
  • blue
  • By reacting the bromopyrogallol red with niobium(V) in a tartrate medium at pH 6.0, an intense blue coloured 3:1 reagent was formed. (wikipedia.org)
  • The reagent is a dark-blue to maroon color when oxidized, and colorless when reduced. (wikipedia.org)
  • common
  • Common oxidants used in this process are permanganate (both sodium permanganate and potassium permanganate), Fenton's Reagent, persulfate, and ozone. (wikipedia.org)
  • System
  • With the HRP-H2O2-BPR catalytic system, the bromopyrogallol red is determined to be an efficient reagent and quantitation of HRP can be performed. (wikipedia.org)