• The retinal transcriptome of WNIN/GR-Ob was compared with the respective lean controls and with the WNIN/Ob model using microarray analysis. (molvis.org)
  • Complementary DNA microarray analysis was performed for gene expression profiling. (thejns.org)
  • For the microarray analysis, we surgically obtained 27 breast cancer tissue samples from patients undergoing treatment at the Saitama Cancer Center Hospital (Saitama, Japan) from 25/8/2003 - 6/11/2003. (iiarjournals.org)
  • b Microarray analysis shows that vascular related genes, among which are Col4a1 and Col4a2 , show the highest significance in late chick lung development. (biomedcentral.com)
  • Results Microarray analysis (n=43) demonstrates that UC patients in remission present an intestinal transcriptional signature that significantly differs from that of non-IBD controls and active patients. (bmj.com)
  • Microarray analysis Microarray analysis of total genome gene expression profiling was performed employing Human 12 x 135 K Gene Expression Array. (trpvantagonists.com)
  • Beyond this time, no differences in global gene expression in infected and control animals were detectable by microarray analysis. (biomedcentral.com)
  • On the other hand, these proteins' expression was significantly downregulated in ARG1 silenced Huh7 cells. (hindawi.com)
  • Compared with non-tumorous tissues, both mRNA expression and protein product of ROR2 and Wnt5a genes were significantly increased in NSCLC. (oncotarget.com)
  • c 2 analysis revealed that high ROR2 or Wnt5a expression in NSCLC was significantly associated with advanced TNM stage. (oncotarget.com)
  • We have performed a microarray expression analysis in late chick lung development and found that COL4A1 and COL4A2 were among the most significantly upregulated genes during the formation of the avian BGB. (biomedcentral.com)
  • SPIB , FOXM1 , and POLR2A significantly regulate all the hub genes. (genominfo.org)
  • Twenty-nine of these genes were significantly regulated in UC-in-remission subjects compared with non-IBD controls, including a large number of epithelial cell-expressed genes such as REG4, S100P, SERPINB5, SLC16A1, DEFB1, AQP3 and AQP8, which modulate epithelial cell growth, sensitivity to apoptosis and immune function. (bmj.com)
  • About half of the genes that are significantly regulated in UC active mucosa remain altered during remission despite endoscopic and histological healing. (bmj.com)
  • Raw areca nut (RAN) consumption induces oral, esophageal and gastric cancers, which are significantly associated with the overexpression of pituitary tumor transforming gene 1/securin and chromosomal instability (CIN). (biomedcentral.com)
  • Results Old age (over 60 years) was found to be significantly associated with SARS-related deaths in the univariate analysis. (besjournal.com)
  • Besides classical gene regulation, miRNAs have emerged as post-transcriptional regulators of angiogenesis. (plos.org)
  • The rat HMGCS2 gene contains elements that control its multihormonal regulation and tissue specificity ( 11 - 14 ). (aacrjournals.org)
  • Even though transcriptional regulation of hsp27 is well recognized [ 14 ], few papers focus on degradation mechanisms of HSP27 protein. (biomedcentral.com)
  • Furthermore, post-transcriptional gene expression regulation by cellular and/or EBV-derived microRNAs has attracted considerable attention. (spandidos-publications.com)
  • Furthermore, we discuss recent findings on EBV associated gastric carcinogenesis by focusing on the roles of latent genes, epigenetic abnormalities, genomic alterations, and post-transcriptional regulation by cellular and viral microRNAs (miRNAs). (spandidos-publications.com)
  • Tumor-infiltrated macrophages were isolated from human GC tissues using magnetic beads, gene transcription was determined by real-time PCR, protein expression was monitored using western blots, metabolites were determined using HPLC, and transcriptional regulation was analyzed by the luciferase-based reporter gene system. (cancerindex.org)
  • Given the strong link between the regulation of some of these genes and the growth and dissemination of gastrointestinal cancers, we believe their aberrant expression in UC may provide a mechanism for epithelial hyper-proliferation and, in the context of malignant transformation, for tumour growth. (bmj.com)
  • The lentivirus-mediated overexpression of psoriasin, mimicking the psoriatic milieu, gave rise to an altered regulation of differentiation genes and an expression pattern reminiscent of that in psoriatic epidermis. (medicaljournals.se)
  • Moreover, direct estrogen regulation is demonstrated for three array-identified genes, Sele , Lypd3 and Arg1 , in mouse cells in vitro . (biomedcentral.com)
  • Fifty-four selected genes were validated in an independent cohort of patients (n=30). (bmj.com)
  • Microarrays are a significant advance because of their small size and are therefore useful when one wants to survey a large number of genes quickly or when the study sample is small. (medscape.com)
  • Microarrays may be used to assay gene expression within a single sample or to compare gene expression in 2 different cell types or tissue samples, such as in healthy versus diseased tissue. (medscape.com)
  • DNA microarrays are simply platforms that consist of small solid supports onto which the sequences from thousands of different genes are attached at fixed locations. (medscape.com)
  • The 2 most common microarray technologies in use are the oligonucleotide microarrays and the robotically spotted complementary DNA (cDNA) microarrays. (medscape.com)
  • Using tissue microarrays and full tissue sections of normal and 448 neoplastic tissues, HE4 immunoreactivity was found in normal glandular epithelium of the female genital tract and breast, the epididymis and vas deferens, respiratory epithelium, distal renal tubules, colonic mucosa, and salivary glands, consistent with HE4 gene expression. (nature.com)
  • Here, we used oligonucleotide and tissue microarrays to survey HE4 gene and protein expression in a large group of malignant and normal tissues from adults. (nature.com)
  • 18 With the exception of the ovarian neoplasms, the same carcinomas analyzed for gene expression were also examined for HE4 by immunohistochemistry on tissue microarrays (see below). (nature.com)
  • The differential expression of a subset of genes was confirmed at the protein level by immunohistochemical labeling of tissue microarrays (inhibin β A [INHBA] and CD29) and/or at the transcript level by RT-PCR (INHBA, AKAP12, ELK3, FOXQ1, EIF5A2, and EFNA5). (elsevierpure.com)
  • Microarrays were then hybridized with Cy3 labeled ds cDNA within a hybridization chamber. (trpvantagonists.com)
  • In a series of 175 human adult tumors, gene expression was highest in ovarian serous carcinomas. (nature.com)
  • The commonly used immunohistochemical panel includes CD31 and CD34 which lack adequate sensitivity and specificity, especially in tumors with epithelioid morphology 2 - 5 . (turkjpath.org)
  • Mice had been sacrificed immediately after 28 days of solutions and tumors have been removed and fixed in 10% neutral buf fered formalin for histologic and immunohistochemical analyses. (trpvantagonists.com)
  • Histological analysis of tumors Tumors were embedded in paraffin, sectioned at five um, and stained with H E. (trpvantagonists.com)
  • Normally, the PVRL4 gene is not active in breast epithelial cells, but its activity is detected in many breast, lung, and ovarian tumors. (elifesciences.org)
  • Here, we examine recent results, including those from the European FP7 project METAcancer consortium, that show that integrated metabolomic analyses can provide information on the stage, subtype and grade of breast tumors and give mechanistic insights. (biomedcentral.com)
  • However, biologically a gene or a sample could participate in multiple biological pathways, and a cellular process is generally active only under a subset of genes or experimental conditions. (columbiagypsy.net)
  • In addition to accumulating more case series of EBV-associated gastric carcinoma (EBVaGC), the development of comprehensive molecular analyses has provided evidence that EBVaGC is a distinct subset both in terms of its clinicopathological and molecular features. (spandidos-publications.com)
  • This cDNA can then be fluorescently labeled and hybridized to the target gene sequences on the microarray. (medscape.com)
  • Applying the BBC algorithm to the yeast expression data, we noticed that most the biclusters we discovered are backed by significant biological evidences, such as for example enrichments of gene features and transcription aspect binding sites in the corresponding promoter sequences. (columbiagypsy.net)
  • Methods Total murine RNA was isolated from the small intestine of a 4-week-old BALB/c mouse (♂).Gene-specific primers were designed and synthesized according to the cDNA sequences of lactase-phlorizin hydrolase (LPH) in human, rat, and rabbit. (besjournal.com)
  • In rats, hepatic ketogenic capacity increases rapidly during postnatal development ( 4 ) or fasting ( 5 ) when the mRNA, protein, and activity of HMGCS2 increase in liver ( 6 - 8 ). (aacrjournals.org)
  • Administration of dibutyryl-cyclic AMP to adult rats increases mRNA, protein levels, and enzymatic activity of HMGCS2 in the liver ( 6, 9 ). (aacrjournals.org)
  • Immunohistochemical analysis showed decreased levels of rhodopsin and postsynaptic density protein-95 (PSD-95) proteins and increased levels of glial fibrillary acidic protein (GFAP), vascular endothelial growth factor (VEGF), and calretinin in WNIN/GR-Ob rats compared with the age-matched lean controls, further supporting cellular stress/damage and retinal degeneration. (molvis.org)
  • Testes from 90-day-old control and flutamide-exposed rats were used for all analyses. (biomedcentral.com)
  • Short-term treatment with flutamide applied to adult rats exerts its primary effect on the basal ES, coexisting junctional complexes and their constituent proteins Cx43 and ZO-1, without any apparent morphological alterations in the seminiferous epithelium. (biomedcentral.com)
  • Objective To characterize the immune reaction in SD rats exposed to trichloroethylene (TCE) and to identify the gene expression profiles involved in skin after TCE exposure. (besjournal.com)
  • Total RNAs of skin samples at 3 h or 24 h after the seventh dose of TCE in SD rats were extracted, and gene expression profiles of these tissues were analyszed by rat toxicology U34 array of Affymetrix. (besjournal.com)
  • [ 1 ] the Cancer Genome Anatomy Project index of tumor genes has classified more than 40,000 genes directly or indirectly involved in one or more cancers. (medscape.com)
  • Proliferation and migration were studied and we used COBRA-FISH and array-CGH for karyotyping and genotyping. (biomedcentral.com)
  • P21-activated kinase 3 (PAK3) is a serine/threonine protein kinase, and PAK3 affects the EMT, proliferation, metastasis and invasion of HCC. (jcancer.org)
  • Lastly, we revealed that addition of type IV collagen protein induced myofibroblast proliferation and migration in monolayer culture and increased the formation of mesenchymal-epithelial septal-like structures in co-culture. (biomedcentral.com)
  • These abnormalities result in significant alterations in gene expression related to cell proliferation, apoptosis, migration and immune signaling pathways. (spandidos-publications.com)
  • Among the genes that remain deregulated during remission, we identify several that are expressed by epithelial cells and are involved in epithelial cell proliferation, resistance to apoptosis and response to stress. (bmj.com)
  • A gain of function screen for genes that enable proliferation independently of matrix anchorage identified a cell adhesion molecule PVRL4 (poliovirus-receptor-like 4), also known as Nectin-4. (elifesciences.org)
  • We show that CCN1 (also known as CYR61), a matricellular protein that dampens and resolves liver fibrosis, also mediates cholangiocyte proliferation and ductular reaction, which are repair responses to cholestatic injury. (jci.org)
  • Administration of CCN1 protein or soluble JAG1 induced cholangiocyte proliferation in mice, which was blocked by inhibitors of NF-κB or NOTCH signaling. (jci.org)
  • The expression of MMR genes (MSH2, MLH1, MSH6 and PSM2) and co-stimulatory molecule CD80 was assessed by tissue microarray immunohistochemistry. (oncotarget.com)
  • Immunohistochemistry for p16 and p53 was performed as well as TP53 and IDH mutation analysis. (biomedcentral.com)
  • Gene and protein expressions were analyzed by real-time RT-PCR and Western blotting, respectively, protein distribution by immunohistochemistry, and steroid hormone concentrations by radioimmunoassay. (biomedcentral.com)
  • REG4, S100P, SERPINB5 and REG1A protein expression was confirmed by immunohistochemistry (n=23). (bmj.com)
  • Double strand cDNA was synthe sized from 5 ug of complete RNA using a SuperScript ds cDNA synthesis kit while in the presence of one hundred pmol oligo dT primers. (trpvantagonists.com)
  • It encodes a protein that prevents separin from promoting sister chromatid separation during mitosis [ 3 ]. (biomedcentral.com)
  • Expression of selected retinal marker genes was studied via real-time PCR. (molvis.org)
  • HE4 is one of more than 8900 different human genes represented on U95a GeneChips (Affymetrix, Santa Clara, CA, USA), which we used previously to develop a molecular classification of 175 human carcinomas based on patterns of gene expression. (nature.com)
  • Using an Affymetrix microarray-based approach we show that the differences in gene expression between male elderly and young human wounds are almost exclusively estrogen regulated. (biomedcentral.com)
  • Since the major variable contributing to chronic wounds in humans is being an aged male [ 4 , 5 ], our initial approach was to compare acute wound gene expression between young and old male human subjects via Affymetrix microarray. (biomedcentral.com)
  • Since the L-ornithine produced by arginase will further metabolize to polyamines, which is involved in the multiple fundamental cellular functions, arginase has been revealed to impact an array of pivotal downstream metabolic pathways [ 6 ]. (hindawi.com)
  • The most responsible genes and pathways involved in the pathology of this disorder have not been fully understood. (genominfo.org)
  • We aimed to identify differentially expressed genes (DEGs), the most critical hub genes, transcription factors, signaling pathways, and biological processes (BPs) associated with the pathogenesis of primary SGC. (genominfo.org)
  • 1). A protein interaction map (PIM) was built, and the main modules within the network were identified and focused on the different pathways and BP analyses. (genominfo.org)
  • Despite many types of investigation that have been performed, the most critical genes and signaling pathways taking part in the etiology of the disease have not been fully demonstrated [ 5 ]. (genominfo.org)
  • What pathways are this gene/protein implicaed in? (cancerindex.org)
  • Conclusions Analysis of the gene signature associated with remission allowed us to unravel pathways permanently deregulated in UC despite histological recovery. (bmj.com)
  • A unified theory of biological aging is emerging in which cellular maintenance and repair systems are influenced by genes and environment, and wound healing is one of the main pathways of such repair responses [ 1 ]. (biomedcentral.com)
  • We were able to definitively map 43 of the 60 ESTs to known or novel genes, and 15 of the ESTs could be localized in close proximity to a gene in the human genome although we were unable to establish that the EST was indeed derived from those genes. (elsevierpure.com)
  • The identification of novel genes that are functionally involved in the initiation and progression of ccRCC may provide more sophisticated early diagnostic and further therapeutic strategies. (biomedcentral.com)
  • Activated ER binds to estrogen-responsive elements (EREs), which are located in the promoter regions of various target genes. (iiarjournals.org)
  • miRNAs (miRs) are 18- to 25-nucleotide long noncoding RNAs that regulate pathophysiological functions via expression of target genes at the posttranscriptional level. (biomedcentral.com)
  • Here, we examined the expression of the HE4 gene and protein in a large series of normal and malignant adult tissues by oligonucleotide microarray and tissue microarray, respectively. (nature.com)
  • HMGCS2 , the gene that regulates ketone body production, is expressed in liver and several extrahepatic tissues, such as the colon. (aacrjournals.org)
  • The GeneExpress® software system Fold Change Analysis Tool was used and 60 ESTs were identified that were expressed at levels at least 3-fold greater in the pancreatic cancers as compared to normal tissues. (elsevierpure.com)
  • An integrated bioinformatics analysis based on the array data from the GEO database was conducted to view the differential expression of CMTM4 across multiple cancers and their corresponding control tissues. (biomedcentral.com)
  • Genomic defects in DNA mismatch repair (MMR) genes (MSH2, MLH1, PSM2 or MSH6) and consequent high-frequency microsatellite instability (MSI) characterize the HNPCC syndrome [ 1 , 2 ]. (oncotarget.com)
  • Searches against the human genomic sequence and comparative genomic analysis of human and mouse genomes was carried out using basic local alignment search tools (BLAST), BLASTN, and BLASTX, for identifying protein coding genes corresponding to the ESTs. (elsevierpure.com)
  • Histological analyses included quantitative immunohistochemical studies and evaluation of collagen deposition. (thejns.org)
  • Histological analysis demonstrated leukocyte and macrophage infiltration in the thrombus at Day 3, myofibroblast infiltration at Days 7 to 14, and progressive collagen deposition and contraction thereafter. (thejns.org)
  • This investigation provides a detailed histological and molecular analysis of thrombus organization in the swine aneurysm model. (thejns.org)
  • We used careful bioinformatics analyses to characterize those ESTs that were found to be highly overexpressed in a series of pancreatic adenocarcinomas. (elsevierpure.com)
  • Fluorescence-activated cell sorting and cDNA-microarray analyses revealed that each subclone was composed of distinct populations of cells. (nature.com)
  • In CaCo-2 colonic epithelial cells, the expression of this gene increases with cell differentiation. (aacrjournals.org)
  • Besides, the level of E-cadherin protein was upregulated in ARG1 knocked down cells. (hindawi.com)
  • Using mouse models, we discovered that mutations in murine Col4a1 and Col4a2 genes affected the balance between lung epithelial progenitors and differentiated cells. (biomedcentral.com)
  • Intracellular psoriasin has been found to interact with various signalling proteins, including the nuclear factor (NF)-kB regulator Jab1, while secreted psoriasin functions as an antimicrobial peptide and has the ability to induce chemotaxis of neutrophils and T cells (6). (medicaljournals.se)
  • Notably, amid the dif ferentially expressed mRNAs predicted to become targeted through the differential miRNAs, had been some genes previously ex perimentally identified to get concerned in aneurysm forma tion or loss of vessel cells, this kind of as TGFBR1, MMPs and IL18 and so on. (trpvantagonists.com)
  • explored which genes enable epithelial cells from the human mammary gland to grow without being attached to the extracellular matrix. (elifesciences.org)
  • They found that the gene that codes for a protein called poliovirus receptor-like 4 (PVRL4) allows attachment-free cell growth and also makes cells cluster together once detached from the matrix. (elifesciences.org)
  • also showed that interactions between the PVRL4 in one cell and a related protein called PVRL1 in a neighboring cell were responsible for holding the cells together in clusters. (elifesciences.org)
  • Conventional molecular target drugs can selectively inhibit gene mutations which activate prolifiration of cancer cells, but many patients in which gene mutations cannot be detected are not targets of the molecular target drugs. (justia.com)
  • Some loss-of-function mutations of genes confer druggable vulnerabilities on cancer cells. (justia.com)
  • Salvia miltiorrhiza could induce hBMSC to differentiate into neuron-like cells.Some differentiated neuron-like cells, that expressed NSE, beta-tubulin and NF-200, showed typical neuron morphology, but some neuron-like cells also expressed alpha smooth muscle protein, making their neuron identification complicated. (besjournal.com)
  • 3 Given its homology and comparable transcription profile with known leukocyte protease inhibitors in the WFDC family of proteins, HE4 also presumably has a role in natural immunity. (nature.com)
  • This gene belongs to the forkhead family of transcription factors which is characterized by a distinct DNA-binding forkhead domain. (cancerindex.org)
  • The BBC model can simply handle lacking data via Monte Carlo imputation and gets the potential to end up being extended to integrated study of gene transcription networks. (columbiagypsy.net)
  • This gene product is involved in cell cycle progression, p53-mediated apoptosis, transcription activator of several other oncogenes [ 4 ] and DNA repair [ 5 ]. (biomedcentral.com)
  • Although several immunohistochemical markers are suggested to assist in the differentiation of HE from AS, evaluation of morphological characteristics such as size, cellularity, nuclear pleomorphism, necrosis and mitotic activity is still essential for an accurate diagnosis 1 . (turkjpath.org)
  • Mutations in this gene cause various glaucoma phenotypes including primary congenital glaucoma, autosomal dominant iridogoniodysgenesis anomaly, and Axenfeld-Rieger anomaly. (cancerindex.org)
  • Scope includes mutations and abnormal protein expression. (cancerindex.org)
  • Loss-of-function mutations of genes encoding subunits of the SWI/SNF chromatin-remodeling complex are found in approximately 20% of all human cancers. (justia.com)
  • However, molecular target drugs cannot be applied to loss-of-function mutations of genes. (justia.com)
  • Therefore, a new therapy has been required for loss-of-function mutations of genes. (justia.com)
  • In most microarray experiments, a significant fraction of the differentially expressed mRNAs identified correspond to expressed sequence tags (ESTs) and are generally discarded from further analyses. (elsevierpure.com)
  • A total of 137 genes were found to be differentially expressed in primary SGC. (genominfo.org)
  • Differentially regulated genes were validated at the level of gene and protein expression, with genes identified as estrogen-regulated in human confirmed as estrogen-dependent in young estrogen depleted mice in vivo . (biomedcentral.com)
  • The scanner that records the intensity value is linked to digital image analysis software, which produces a color-coded image of the array, and a quantitative value is recorded for each target gene. (medscape.com)
  • [ 6 ] Both technologies use hybridization of labeled nucleic acid transcripts to measure the gene expression. (medscape.com)
  • Moreover, the FD10 microarray system automates and integrates pumping of the sample solution for hybridization, solution-driven incubation, and image acquisition and analysis functions ( 8 ). (iiarjournals.org)
  • Hybridization time in this system is 2 hours, an improvement over the 16 hours required with a standard glass array. (iiarjournals.org)
  • After hybridization and washing, the slides have been scanned applying the Axon Gene Pix 4000B microarray scanner. (trpvantagonists.com)
  • The present invention relates to methods, kits and systems for predicting the benefit from inclusion of taxane in a chemotherapy regimen based on the measurements of gene expression levels in tumor samples of breast cancer patients. (justia.com)
  • Background: Expression analysis of estrogen response genes (ERGs) may help to predict the effectiveness of endocrine therapies in breast cancer patients. (iiarjournals.org)
  • We selected the ERGs for this study from our previously developed custom-made breast cancer cell cDNA glass array ( 6 ). (iiarjournals.org)
  • Our data have provided evidence for a comparable prediction of clinical outcome in CMF-treated breast cancer patients using conventional clinical variables and gene expression based markers. (lu.se)
  • Data were analyzed by cluster analysis. (iiarjournals.org)
  • First, Ezetimibe inhibitor a gene or an experimental condition can be assigned to only one cluster. (columbiagypsy.net)
  • In addition, the gene that encodes psoriasin is positioned within the epidermal differentiation complex (EDC) (10-12), a gene cluster that contains several genes of importance in the terminal differentiation of the human epidermis (10, 11). (medicaljournals.se)
  • Additionally, overexpression of ARG1 led to a significant increase in the expression of Vimentin, N-cadherin, and β -catenin both at protein and mRNA levels, which promotes the EMT process. (hindawi.com)
  • By omic data analysis, we found a substantial downregulation of CMTM4 in ccRCC. (biomedcentral.com)
  • miRNAs comprise a large group of endogenous non-coding RNAs that can block mRNA translation or negatively regulate mRNA stability and thereby play a central role in regulating gene expression [10] , [11] . (plos.org)
  • The differentiation-induced psoriasin expression was found to be mediated by the protein kinase C pathway. (medicaljournals.se)
  • CD28, Mitogen activated protein kinase 1. (trpvantagonists.com)
  • Activated phospho-kinases involved in exosome-treated adipocytes were detected by phospho-kinase antibody array and Western blot. (biomedcentral.com)
  • Multivariate analyses suggested that ROR2, Wnt5a and TNM stage were independent prognostic factors in NSCLC. (oncotarget.com)
  • Chi-square test and multivariate stepwise logistic regression analysis were performed. (besjournal.com)
  • Multivariate stepwise logistic regression analysis found four risk factors for SARS-related deaths. (besjournal.com)
  • Recent advances in genome-wide and comprehensive molecular analyses have demonstrated that both genetic and epigenetic changes contribute to EBVaGC carcinogenesis. (spandidos-publications.com)
  • Global CpG island hypermethylation, which induces epigenetic silencing of tumor suppressor genes, is also a unique feature of EBVaGC and is considered to be crucial for its carcinogenesis. (spandidos-publications.com)
  • Since the molecular mechanism underlying securin upregulation remains unclear, this study intended to investigate the association of securin upregulation with the Rb-E2F1 circuit and epigenetic histone (H3) modification patterns both globally and in the promoter region of the securin gene. (biomedcentral.com)
  • Psoriasin, which is highly expressed in psoriasis, is encoded by a gene located within the epidermal differentiation complex. (medicaljournals.se)
  • CMTM4 is a member of this family and is located at chromosome 16q22.1, a locus that harbours a number of tumour suppressor genes. (biomedcentral.com)
  • Knowledge of the expression patterns of HE4 in our survey is useful for application in histopathologic diagnosis, and should be taken into consideration in future studies that examine the role of HE4 as a serological tumor biomarker or as a target for gene-based therapy. (nature.com)
  • While expression patterns of many proteins in human medulloblastoma subgroups have been discerned, the expression pattern of GPCRs in medulloblastoma has not been investigated. (biomedcentral.com)
  • Inflammasomes refer to the cytoplasmic multimeric protein complexes that sense pathogen- or danger-associated molecular patterns (PAMPs or DAMPs, respectively) to mediate the inflammatory response and induce programmed cell death known as pyroptosis [ 1 ]. (thno.org)
  • For that reason, 10 decided on wheat accessions with different ploidy amount as well as yr regarding discharge, in the past indicated because of their ATI gene patterns, were produced during a few sequential crop years from a couple of developing places and also employed for within vitro ATI pursuits. (talazoparibinhibitor.com)
  • HA proteins bind to sialoside receptors on the host cell surface. (cdc.gov)
  • Consistent with increase in serum T, piperine increased Leydig cell number, cell size, and multiple steroidogenic pathway proteins, including steroidogenic acute regulatory protein, cholesterol side-chain cleavage enzyme, 3β-hydroxysteroid dehydrogenase 1, 17α-hydroxylase/20-lyase, and steroidogenic factor 1 expression levels. (frontiersin.org)
  • Based on these findings, we propose that ketogenesis is an undesirable metabolic characteristic of the proliferating cell, which is down-regulated through c-Myc-mediated repression of the key metabolic gene HMGCS2 . (aacrjournals.org)
  • Full length human recombinant protein of human IDO1 produced in HEK293T cell. (thermofisher.com)
  • Present study was designed to establish a causal connection between changes in the cell-cell junction protein expression at the blood-testis barrier and alterations in the adult rat testis histology following an anti-androgen flutamide exposure. (biomedcentral.com)
  • Synthetic lethality," which is defined by an interdependent relationship between two genes, which means that simultaneous loss of two genes, but not loss of either gene alone, leads to cell death. (justia.com)
  • These genes participate in biological functions such as cellular growth, movement, assembly and organisation, as well as in fatty acid and protein metabolism. (bmj.com)
  • Each of these layers is characterized by specific features in terms of gene expression, function and cellular mor-phology (1). (medicaljournals.se)
  • Multiple processes have been implicated in age-related delayed healing, including altered gene expression, intrinsic cellular changes, and changes in extracellular milieu (including hormones). (biomedcentral.com)
  • Multiple processes have been implicated in cutaneous aging, including gene expression, intrinsic cellular change and an altered extracellular milieu. (biomedcentral.com)
  • The SWI/SNF complex was originally described in yeast as the protein complex critical for cellular responses to mating-type switching (SWI) or sucrose fermentation (SNF). (justia.com)
  • The introduction of complementary DNA (cDNA) microarray technology in 1995 has helped to facilitate the identification and classification of DNA sequence information and the assignment of functions to these new genes by allowing investigators to analyze expression of thousands of genes simultaneously in a single experiment. (medscape.com)
  • A confocal scanner then reads the fluorescent intensity of each hybridized sequence in the array. (medscape.com)
  • A coding sequence (CDS) fragment was obtained using RT-PCR, and inserted into a clone vector pNEB-193, then the cDNA was sequenced and analyzed using bioinformatics. (besjournal.com)
  • Analysis of the deduced amino acid sequence using bioinformatics revealed that this cDNA shared extensive sequence homology with human LPH containing a conserved glycosy1 hydrolase family 1 motif important for regulating lactase intolerance. (besjournal.com)
  • Because the locations of the probes are known, the intensity and pattern of the labeled mRNA can be used to measure the expression of the targeted gene. (medscape.com)
  • The aRNAs were applied to the infrastructure of a 3DMS with spotted cDNA probes to 36 ERGs. (iiarjournals.org)
  • Through database analysis, we found that PAK3 was highly expressed in HCC patients and was positively correlated with tumor stage and grade, suggesting that PAK3 expression was closely related to HCC occurrence and development. (jcancer.org)
  • How Reliable Are Gene Expression-Based and Immunohistochemical Biomarkers Assessed on a Core-Needle Biopsy? (lu.se)
  • 54 genes and eleven miRNAs have been concerned from the top 12 predicted functions, and a network produced by IPA showed the interactions amongst individuals miRNAs and mRNA. (trpvantagonists.com)
  • Some distinctive genes proven while in the network targeted by a lot more than 3 miRNAs include things like Kruppel like things 4. (trpvantagonists.com)
  • [ 2 , 3 ] Conventional techniques of gene investigation in cancer rely on the identification of single genetic alterations associated with disease. (medscape.com)
  • In contrast to ancestral clade 2.3.4 H5 proteins, novel clade 2.3.4.4 H5 proteins bind to fucosylated sialosides because of substitutions K222Q and S227R, which are unique for highly pathogenic influenza virus H5 proteins. (cdc.gov)
  • Although a recent study ( 22 ) reported enhanced avidity of H5N6 viruses for human-type receptors, recombinant clade 2.3.4.4 highly pathogenic influenza A virus H5 proteins from virus isolates in North America show a strict avian receptor-binding preference ( 23 ). (cdc.gov)
  • We hypothesized that analysis of GPCR expression would identify clear subsets of medulloblastoma and suggest distinct GPCRs that might serve as molecular targets for both imaging and therapy. (biomedcentral.com)
  • The lists of up- and downregulated genes were cross-matched with lists of genes known to be associated with cytokines or the extracellular matrix. (thejns.org)
  • Therefore, it is quite critical to identify relevant genes and novel targets for therapy of HCC. (hindawi.com)
  • This study illustrated several hub genes and their master regulators that might be appropriate targets for the therapeutic aims of primary SGC. (genominfo.org)
  • Conclusion BALB/c mouse LPH cDNA (GenBank accession No: AY751548) provides a necessary foundation for study of the biological function and regulatory mechanism of the lactose intolerance in mice. (besjournal.com)
  • Many investigators consider the expression of estrogen-responsive genes (ERGs) to be evidence of an activated, functional ER ( 6 , 7 ). (iiarjournals.org)
  • In a recent study we characterized estrogen-regulated changes in gene expression using a model of delayed wound healing in young mice that have been rendered hypogonadal by ovariectomization (hence removing any effects of 'intrinsic aging') [ 9 ]. (biomedcentral.com)
  • Thus, using comparative analysis we are now in a position to address the relative contributions of estrogen and aging to healing in elderly humans. (biomedcentral.com)
  • Transcriptomic alterations of exosome-stimulated adipocytes were analyzed using gene expression profiling, and secretion of inflammation-associated cytokines was detected by RT-PCR and ELISA. (biomedcentral.com)