• In the RRBP1 -knockdown Calu-6 cells, a human renin-producing cell line, transmission electron and confocal microscopy revealed that renin was primarily retained in the endoplasmic reticulum and was unable to efficiently target the Golgi apparatus for secretion. (biomedcentral.com)
  • B-D , Images from triple-labeling experiments in sections from GFP-GAD67 mice showing the colocalization of α2 subunit-positive clusters (red) with gephyrin (blue) inside eGFP-positive GC dendrites (green) in the EPL, as visualized by confocal microscopy. (jneurosci.org)
  • Technical expertise include cell and tissue culture, Immunofluorescence, immunoelectron microscopy, transmission- and scanning electron microscopy, Laser-scanning confocal microscopy, computer image analysis. (marketopia.co)
  • We, for the primary time, aimed to analyze the distribution, immunohistochemical traits, and ultrastructure of PDGFRα -positive ICs in murine vas deferens, utilizing confocal laser scanning microscopy, transmission electron microscopy (TEM), and immuno-electron microscopy (immuno-EM). (aetox.com)
  • 3) The cytoplasmic and membrane spanning domains of galactosyltransferase were found to be sufficient to retain all of the hybrid invariant chain in trans Golgi cisternae as judged by indirect immunofluorescence, treatment with brefeldin A and immuno-electron microscopy. (comparewords.com)
  • Strategies Immuno-electron microscopy 4 ml of mid-log-phase EATRO 1125 wild-type procyclic cells had been gathered by centrifugation, 5 min 1,000 g. (isme-la2019.org)
  • In immunofluorescence technique, the synthesized antibodies are complexed with fluorochromes that emit low wavelength radiation, which is spontaneous, that can be visualized by an ultraviolet microscope. (adejournal.com)
  • Immunofluorescence microscopy utilizes antibodies that are labeled with fluorescent dye. (genomicglossaries.com)
  • For immunofluorescence, we used antibodies in opposition to PDGFRα and different markers of ICs. (aetox.com)
  • A , Low-magnification photomicrograph depicting the distribution and morphology of eGFP-positive newborn GCs in adult mouse OB at 7 dpi, as detected by combined eGFP and immunofluorescence with antibodies against GFP. (jneurosci.org)
  • Epstein, A.L. and Clevenger, C.V., Identification of nuclear antigens in human cells by immunofluorescence, immunoelectron microscopy, and immuno-biochemical methods using monoclonal antibodies. (neobiotechnologies.com)
  • Immune electron microscopy (more often called immunoelectron microscopy) is the equivalent of immunofluorescence, but it uses electron microscopy rather than light microscopy. (wikipedia.org)
  • Scanning electron microscopy is a viable option if the antigen is on the surface of the cell, but transmission electron microscopy may be needed to see the label if the antigen is within the cell. (wikipedia.org)
  • Transmission electron microscopy then detects the antibody and, therefore, the protein. (wikipedia.org)
  • While using immune electron microscopy, the specimen can either be in thin sections so the electrons can penetrate it or negatively stained. (wikipedia.org)
  • When used in immune electron microscopy, negative staining implants a small particle into the specimen, better resolving structures within it. (wikipedia.org)
  • Results from immune electron microscopy are typically quantified visually. (wikipedia.org)
  • During successful experiments, immune electron microscopy can accurately locate proteins and strengthen comprehension of the relationship between structure and function. (wikipedia.org)
  • This invention led to the scanning electron microscope and transmission electron microscope, which later contributed to immunoelectron microscopy. (wikipedia.org)
  • Transmission electron microscopy successfully provides general information about structure but struggles to differentiate more detailed parts of a virus or cell. (wikipedia.org)
  • BACKGROUND: Accurate diagnosis of epidermolysis bullosa (EB) has significant implications for prognosis, management, and genetic counseling.OBJECTIVE: To describe diagnostic testing patterns and assess diagnostic concordance of transmission electron microscopy (TEM), immunofluorescence mapping (IFM), and genetic analysis for EB.METHODS: A retrospective cohort of patients enrolled in the Epidermolysis Bullosa Clinical Characterization and Outcomes Database from January 1, 2004 to July 8, 2019 was included. (stanfordchildrens.org)
  • Visual and photographic microscopy in which electron beams with wavelengths thousands of times shorter than visible light are used in place of light, thereby allowing much greater magnification. (genomicglossaries.com)
  • In high-resolution electron microscopy one can begin to do `` crystallography without crystals'', averaging thousands of images of single molecules or other assemblies to reveal near atomic level structure. (genomicglossaries.com)
  • Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana- Champaign Molecular Biomedicine in the Era of Teraflop Computing - DDDAS.org Narrower term: transmission electron microscopy (TEM). (genomicglossaries.com)
  • Microscopy in which the samples are first stained immunocytochemically and then examined using an electron microscope. (genomicglossaries.com)
  • This study elucidated the etiology of C3 glomerulonephritis (C3GN) and non-C3GN with primary membranoproliferative glomerulonephritis (MPGN) using transmission electron microscopy (TEM) and periodic acid-methenamine silver stain (PAM-EM). (bvsalud.org)
  • In contrast to the classical binary fission used by most bacteria, electron microscopy reveals that ROLP exploits an asymmetrical budding strategy to replicate in plant hosts and insect vectors. (biomedcentral.com)
  • In the present study, we investigated the localization of PRKRA in the mammalian testis by immunofluorescence and immunoelectron microscopic techniques. (hindawi.com)
  • The capacity of mouse CABYR isoforms to associate into dimers and oligomers, and the relationships between CABYR and other FS proteins were studied by gel electrophoresis, Western blotting, immunofluorescence, immunoprecipitation and yeast two-hybrid analyses. (biomedcentral.com)
  • Previously, we investigated the localization of several nuage-resident proteins in the nuage structures of spermatogenic cells by immunoelectron microscopy (IEM) [ 22 - 26 ]. (hindawi.com)
  • The development of new probe technologies, such as quantum dots and high-resolution laser fluorescence microscopy, allow real- time observations of molecular interactions and trafficking within living cells. (genomicglossaries.com)
  • total internal reflection fluorescence microscopy. (rupress.org)
  • Immunoelectron microscopy using a polyclonal anti-P-selectin antibody demonstrated immunogold localization in dense granules, morphologically indistinguishable from the ACTH granules. (rupress.org)
  • B) GAMA localization using immunoelectron microscopy. (huji.ac.il)
  • In today's research, we characterized the DEV pUL51 subcellular localization by pc aided analysis, aswell as indirect immunofluorescence (IIF) and transmitting immunoelectron microscopy (TIEM) strategies in DEV-infected cells. (e-core.org)
  • Sometimes, more advanced techniques may be necessary to establish the diagnosis such as split skin immunoblotting, immunoelectron-microscopy, and enzyme-linked immunosorbent assay (ELISA). (iacdworld.org)
  • Enzyme-linked immunosorbent assay (ELISA) has also been suggested as a useful diagnostic method in suspected cases of pemphigus erythematosus with equivocal immunofluorescence results. (medscape.com)
  • In this study, a specific antibody against the immunodominant membrane protein (Imp) of rice orange leaf phytoplasma (ROLP) was generated and subjected to the frozen section immune gold labeling assay, immune gold labeling microscopy, and immune fluorescence labeling assay to investigate how ROLP enters, propagates, and spreads in rice and its leafhopper vector Recilia dorsalis at the ultrastructural level. (biomedcentral.com)
  • Immunofluorescence allows the imaging of a particular factor in cells or tissue sections through the use of a specific antibody that is conjugated with a fluorescent dye. (iacdworld.org)
  • Alpha-gal epitopes in endosomes of the digestive gut cells of the ticks were observed via immunoelectron microscopy. (lih.lu)
  • In HEK293T cells, immunoelectron microscopy confirmed the association of MxA with endosomes, and immunofluorescence methods showed these to be positive for early endosome markers (early endosomal antigen 1, clathrin light chain and Rab5) and RSmad1/5/8. (touro.edu)
  • Indirect immunofluorescence staining (IIF) has a secondary antibody labeled with a fluorochrome used to recognize a primary antibody. (iacdworld.org)
  • Direct immunofluorescence microscopy performed on epithelial biopsy specimen obtained from a patient with pemphigus vulgaris detects immunoglobulin G deposits at the epithelial cell surfaces. (medscape.com)
  • With direct immunofluorescence (see the image above) in pemphigus erythematosus, linear deposits of immunoglobulin G (IgG) and C3 are present in the intercellular space of the epidermis. (medscape.com)
  • With immunoelectron microscopy in pemphigus erythematosus, IgG and C3 deposits are localized to the epidermal cell membranes and the upper dermis. (medscape.com)
  • Immunoelectron microscopy identifies and localizes a molecule of interest, specifically a protein of interest, by attaching it to a particular antibody. (wikipedia.org)
  • Direct immunofluorescence staining (DIF) has a primary antibody labeled with a fluorescence dye. (iacdworld.org)
  • Immunoelectron microscopy was used to investigate background labelling and the specificity of the antibody to antigenic sites. (alr-journal.org)
  • This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. (antikoerper-online.de)
  • Microscopy of specimens stained with fluorescent dye (usually fluorescein isothiocyanate) or of naturally fluorescent materials, which emit light when exposed to ultraviolet or blue light. (genomicglossaries.com)
  • Immunofluorescence and immunoelectron microscopy experiments established that AeIMUC1 is a bona fide PM protein, and binding of the recombinant protein to chitin was demonstrated in vitro. (johnshopkins.edu)
  • The subject of the study was the major fibrous sheath protein of the mouse sperm flagellum, AKAP82, a member of the A Kinase Anchor Protein (AKAP) family of polypeptides that bind the regulatory (RII) subunit of protein kinase A (PK-A). Immunoelectron microscopy demonstrated that AKAP82 is present throughout the transverse ribs and longitudinal columns of the fibrous sheath. (elsevierpure.com)
  • A , Immunofluorescence staining depicting the distribution of α2 subunit clusters in adult mouse OB. (jneurosci.org)
  • Immunofluorescence permit early diagnosis, treatment and subsequent monitoring of disease activity in patient with potentially life threatening disease. (adejournal.com)
  • Their diagnosis is aided by specific immunofluorescence testing. (iacdworld.org)
  • The glide was previously HLCL-61 covered with poly-L-Lysine for 10 min as found in regular immunofluorescence methods C find [10]. (isme-la2019.org)
  • By double-label immunofluorescence, P-selectin was visible as a punctate pattern at the tips of cell processes. (rupress.org)
  • Alpha-gal epitopes were detected in the midgut, hemolymph and salivary glands, and the immunofluorescence analysis revealed signs of the endocytosis of alpha-gal-containing constituents during the process of hematophagy. (lih.lu)
  • Rapid freezing and freeze substitution were used in conjunction with immunofluorescence, whole mount EM, and immunoelectron microscopy to study the organization of myosin and actin in growth cones of cultured rat superior cervical ganglion neurons. (wustl.edu)
  • Immunoelectron microscopy of myosin using postembedment labeling with colloidal gold showed a similar distribution to that seen by immunofluorescence. (wustl.edu)
  • By immunofluorescence, pro-AKAP82 was localized to the entire length of the principal piece in testicular sperm, while in epididymal sperm p109 and p26 were present only in the proximal portion of the principal piece. (elsevierpure.com)
  • A type of scanning probe microscopy in which a probe systematically rides across the surface of a sample being scanned in a raster pattern. (genomicglossaries.com)