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  • NS4A
  • Regarding NS3 protease, two strategies have been followed: competitive inhibitors blocking the active site and allosteric inhibitors blocking the binding of the accessory viral protein NS4A. (jove.com)
  • protease
  • These include targeting the recognition molecules for the avoidance of detection, targeting key enzymes and complexes of the complement pathways like C3 convertases and C5b-9 formation - either by encoding complement regulators or by recruiting membrane-bound and soluble host complement regulators, cleaving complement proteins by encoding protease, and inhibiting the synthesis of complement proteins. (frontiersin.org)
  • We found ligands binding to the Zn(+2)-free NS3 protease, trap the inactive protein, and block the viral life cycle. (jove.com)
  • HCV NS3/4A protein is an attractive therapeutic target responsible for harboring serine protease and RNA helicase activities during the viral replication. (jove.com)
  • Small molecules binding at the interface between the protease and helicase domains can stabilize the closed conformation of the protein and thus block the catalytic function of HCV NS3/4A protein via an allosteric regulation mechanism. (jove.com)
  • Occludin
  • These are the tetraspanin CD81, scavenger receptor class B member I (SR-BI), and the tight junction proteins Claudin-1 and Occludin [ 9 - 12 ]. (hindawi.com)
  • The fully-developed HLCs expressed phase I, II, and III drug-metabolizing enzymes, HCV associated receptors (claudin-1, occludin, CD81, ApoE, ApoB, LDL-R) and HCV essential host factors (miR-122 and SEC14L2) comparable to the primary human hepatocyte. (biomedcentral.com)
  • genome
  • These trVLPs can continuously infect cells expressing the Ebola virus proteins responsible for genome replication and transcription, allowing us to safely model multiple infectious cycles under biosafety level 2 conditions. (jove.com)
  • gene
  • To date, several host- and virus-related factors have been implicated in the progression to MCII, such as the virus-induced expansion of selected subsets of B-cell clones expressing discrete immunoglobulin variable (IgV) gene subfamilies, the involvement of complement factors and the specific role of some HCV proteins. (mdpi.com)
  • capsid
  • The two 5-proximal ORFs encode the replicases, two internal ORFs encode movement proteins, and the 3-proximal ORF encodes a polypeptide (p37) which plays a dual role as capsid protein and as suppressor of RNA silencing. (jove.com)
  • promotes
  • NSs induces a shut-off of host transcription including interferon (IFN)-beta mRNA 7,8 and promotes degradation of double-stranded RNA-dependent protein kinase (PKR) at the post-translational level. (jove.com)
  • cells
  • Due to the relatively conserved binding region of E2 to the CD81 receptor on the liver cells, this discovery is expected to pave the way to design a HCV vaccine which will stimulate antibody response with neutralizing effects on broad range of virus strains. (wikipedia.org)
  • The green fluorescent protein is a commonly used marker for studying lentiviruses in living cells. (jove.com)
  • CD81 cross-linking by monoclonal antibody (mAb) specific for CD81 or by immobilized E2 have been shown to result in costimulatory signals for human T cells. (pubmedcentralcanada.ca)
  • In this study, we show that CD81 cross-linking via immobilized E2 or mAbs specific for CD81 inhibits not only non major histocompatibility complex-restricted cytotoxicity mediated by NK cells but also interferon (IFN)-γ production by NK cells after exposure to interleukin (IL)-2, IL-12, IL-15, or CD16 cross-linking. (pubmedcentralcanada.ca)
  • These results show that CD81 cross-linking mediates completely different signals in NK cells versus T cells. (pubmedcentralcanada.ca)
  • andsubsequently administering at least once a second composition comprising a viral vector comprising a nucleic acid encoding said at least one epitope of said first composition, whereby the nucleic acid of said second composition is expressed in one or more cells of the subject and at least part of the protein of said first composition is produced. (patentsencyclopedia.com)
  • complex
  • This immune complex-mediated vasculitis is characterized by a primary B-cell clonal proliferation accompanied by the deposition of immune complexes composed of complement factors, mono/oligoclonal IgMs with rheumatoid factor (RF) activity bound to oligo/polyclonal IgGs that, in the case of HCV infection, are mostly directed against HCV proteins [ 3 ]. (mdpi.com)
  • They include: apo state of HCV NS3/4A protein, HCV NS3/4A protein in complex with an allosteric inhibitor and the truncated form of the above two systems. (jove.com)
  • The molecular dynamics simulation results indicate HCV NS3/4A protein in complex with the allosteric inhibitor 4VA adopts a closed conformation (inactive state), while the truncated apo protein adopts an open conformation (active state). (jove.com)
  • allosteric
  • Here, we aimed to provide some insight into the inhibitor binding mode and allosteric regulation mechanism of HCV NS3/4A protein by using computational methods. (jove.com)
  • Finally, a dynamic model about the allosteric regulation and conformational changes of HCV NS3/4A protein was proposed and could provide fundamental insights into the allosteric mechanism of HCV NS3/4A protein function regulation and design of new potent inhibitors. (jove.com)
  • function
  • This method may also allow analysis of how PP pDCs affect the frequency or function of other immune subsets in PPs. (jove.com)
  • infection
  • SEC14L2, an α-tocopherol transfer protein, was expressed in HLCs, but not in Huh7 cell, had been implicated in effective HCVser infection. (biomedcentral.com)
  • play an important role
  • These proteins are highly glycosylated and play an important role in cell entry. (biomedcentral.com)
  • Further residue interaction network analysis suggests the communication of the domain-domain interface play an important role in the transition from closed to open conformation of HCV NS3/4A protein. (jove.com)
  • present
  • In particular, about 60% of HCV-infected patients present cold-precipitable (cryoprecipitable) and noncryoprecipitable immune complexes that could be associated with the clinical onset of type II mixed cryoglobulinemia (MCII) [ 2 ]. (mdpi.com)
  • The methods utilize one or more administrations of HCV protein compositions, followed by one or more administrations of a viral vector comprising a nucleic acid encoding a least one HCV epitope that is present in the first composition. (patentsencyclopedia.com)
  • human
  • Human Leukocyte Antigen (HLA) Class I Down-Regulation by Human Immunodeficiency Virus Type 1 Negative Factor (HIV-1 Nef): What Might We Learn From Natural Sequence Variants? (mdpi.com)
  • Therefore, HBV and HBV-derived proteins were examined for their effect on human pDC in vitro. (jove.com)
  • contain
  • Negative-stain electron microscopy revealed that the particles were spherical (~40- to 75-nm diameter) and pleomorphic and that some of them contain HCV E2 protein and apolipoprotein E on their surfaces. (pubmedcentralcanada.ca)