• assays
  • The species examined in this study included the white-spotted puffer (Arothron hispidus), the Japanese sea bass (Lateolabrax japonicus), olive flounder (Paralichthys olivaceus) and the "sting fish" or Schlegel's black rockfish (Sebastes schegeli) Several serologic assays have been developed to identify LCDV infections, including flow cytometry, immunoblot, and immunofluorescence. (wikipedia.org)
  • cell
  • We identified multiple subpopulations across T, B, and NK-cell lineages that distinguished patients with cGVHD from those without cGVHD and which were associated in varying ways with severity of cGVHD. (frontiersin.org)
  • Finally, no differences in levels of blood soluble markers could be identified, with the exception for the semi-soluble combined marker B-cell activating factor/B cell ratio, which was increased in patients with mild cGVHD compared to patients without cGVHD. (frontiersin.org)
  • The clinical consequences of the exercise induced immune changes have not formally been identified, but the exercise effect on lymphocyte dynamics and immune function may be linked to the exercise effects on resistance to infections and malignancy and the cytokine response may be linked to muscle damage or muscle cell growth. (bmj.com)
  • Similar cell cycle compartments are also recognized by multiparameter analysis that includes measurement of expression of cyclin D1, cyclin E, cyclin A and cyclin B1, each in relation to DNA content Concurrent measurement of DNA content and of incorporation of DNA precursor 5-bromo-2'-deoxyuridine (BrdU) by flow cytometry is an especially useful assay, that has been widely used in analysis of the cell cycle in vitro and in vivo. (wikipedia.org)
  • At the same time, microfluidic qPCR methods are providing a flow cytometry-like method of quantifying 48 or more RNA molecules per cell. (wikipedia.org)
  • Blood counting machines aspirate a very small amount of the specimen through narrow tubing followed by an aperture and a laser flow cell. (wikipedia.org)
  • Mack Fulwyler was the inventor of the forerunner to today's flow cytometers - particularly the cell sorter. (wikipedia.org)
  • Soon after, flow cytometry instruments were developed, including the Cytofluorograph (1971) from Bio/Physics Systems Inc. (later: Ortho Diagnostics), the PAS 8000 (1973) from Partec, the first FACS (Fluorescence-activated cell sorting) instrument from Becton Dickinson (1974), the ICP 22 (1975) from Partec/Phywe and the Epics from Coulter (1977/78). (wikipedia.org)
  • Modern flow cytometers are able to analyze many thousand particles per second, in "real time," and, if configured as cell sorters, can actively separate and isolate particles at similar rates having specified optical properties. (wikipedia.org)
  • A flow cytometer has five main components: a flow cell, a measuring system, a detector, an amplification system, and a computer for analysis of the signals. (wikipedia.org)
  • widely
  • Data standards include the widely adopted Flow Cytometry Standard (FCS) defining how data from cytometers should be stored, but also several new standards under development by the International Society for Advancement of Cytometry (ISAC) to aid in storing more detailed information about experimental design and analytical steps. (wikipedia.org)
  • cytometer
  • Each fluorescently labeled bead is analyzed in a flow cytometer, resulting in a separation of mutant from wild-type DNA as well as the ratio of mutant to wild-type DNA present in a sample. (wikipedia.org)
  • At the 5th American Engineering Foundation Conference on Automated Cytology in Pensacola (Florida) in 1976 - eight years after the introduction of the first fluorescence-based flow cytometer (1968) - it was agreed to commonly use the name "flow cytometry", a term that quickly became popular. (wikipedia.org)
  • Acquisition is mediated by a computer physically connected to the flow cytometer, and the software which handles the digital interface with the cytometer. (wikipedia.org)
  • specific
  • These disorders can be further subdivided into B- and Tcell disorders by using specific antibodies in a test process, known as immunophenotyping, which can be performed by flow cytometry on peripheral blood or bone marrow aspirates, or by staining paraffin sections using immunohistochemical techniques. (thefreelibrary.com)
  • Molecular characterization of this process identified a specific DNase (CAD, caspase-activated DNase) that cleaves chromosomal DNA in a caspase-dependent manner. (wikipedia.org)
  • signals
  • Identifying signals of inflammation initiation and responses to stimuli from within the epithelial layer is critical to understanding the molecular pathways underlying disease pathology. (dovepress.com)
  • immune
  • Traditionally, scientists studying the immune system have had to search for antigens on an individual basis and identify the protein sequence of these antigens ("epitopes") that would stimulate an immune response. (wikipedia.org)
  • early
  • Early flow cytometers were, in general, experimental devices, but technological advances have enabled widespread applications for use in a variety of both clinical and research purposes. (wikipedia.org)
  • techniques
  • Phagocytic uptake was detected using flow cytometric and microscopic techniques. (jimmunol.org)
  • Flow cytometry bioinformatics requires extensive use of and contributes to the development of techniques from computational statistics and machine learning. (wikipedia.org)
  • cause
  • This information can be very helpful to a physician who, for example, is trying to identify the cause of a patient's anemia. (wikipedia.org)