• Fine fescue chloroplast genome sizes ranged from 133,331 to 133,841 bp and contained 113-114 genes. (umn.edu)
  • Genome-wide expression of low temperature response genes in Rosa hybrida L.. Plant Physiology and Biochemistry. (usda.gov)
  • DNA barcoding begins with extracting the DNA from a material and then amplifying target regions of the genome (genes) that will allow differentiation. (nutraceuticalsworld.com)
  • Those genes are then sequenced for identification. (nutraceuticalsworld.com)
  • Genes that have been traditionally sequenced for identification of plants include ribulose-1, 5-biphosphate carboxylase (rbcL), and maturase K (matK). (nutraceuticalsworld.com)
  • A total of 134 genes were identified in the cp genome of S. tetrandra, including 87 protein - coding genes , 8 rRNA genes , 37 tRNA genes and 2 pseudogene copies (ycf1 and rps19). (bvsalud.org)
  • Comparative analysis of the Stephania cp genomes revealed 5 highly variable regions, including 3 intergenic regions (trnH-psbA, trnD-trnY, trnP) and two protein coding genes (rps16 and ndhA). (bvsalud.org)
  • Phylogenetic tree analysis based on the conserved chloroplast protein coding genes indicated a sister relationship between S. tetrandra and the monophyletic group of S. japonica and S. kwangsiensis with high support values, suggesting a close genetic relationship within Stephania plants . (bvsalud.org)
  • A total of 114 genes have been identified in the genome, including 80 protein-coding genes, 30 tRNA genes, and 4 rRNA genes. (kib.ac.cn)
  • Genome-wide identification and expression analysis of the bZIP transcription factor family genes in response to abiotic stress in Nicotiana tabacum L. (biomedcentral.com)
  • In this study, 23 HD-Zip genes distributed on 6 chromosomes were identified from the peach ( Prunus persica ) genome, and named PpHDZ01-23 according to their positions on the chromosomes. (biomedcentral.com)
  • The 2.01 Mb genome with a G+C content of 51.9% was assembled into 221 contigs and predicted to have 1,913 protein-coding genes, 98 pseudo genes, 7 rRNAs, 60 tRNAs, and 1 CRISPR array. (bvsalud.org)
  • All plastomes are highly conserved in genome structure and gene order, with a total length of 158-159 kb and 122 genes. (biomedcentral.com)
  • Because the mitochondrial cytb gene potentially represents a new standard method for identifying Prototheca species ( 5 ), we performed whole-genome sequencing to investigate cytb as well as other genes by using Illumina MiSeq ( https://www.illumina.com ) ( Appendix ). (cdc.gov)
  • However, the number of variable sequences provided by chloroplast DNA for suggested genes or genomic regions in plant phylogenetic analyses is often inadequate. (geneticsmr.com)
  • The prin2.2 and csp41b-2 single mutants displayed pale phenotypes, abnormal chloroplasts with reduced transcript levels of photosynthesis genes and defects in embryo development. (frontiersin.org)
  • The gradual conversion from endosymbiont to organelle during the course of evolution has clearly been accompanied by a dramatic reduction in genome size as the chloroplasts lost most of their genes to the nucleus. (frontiersin.org)
  • The genome contains 92 protein-coding genes, 36 transfer RNA genes, eight ribosomal RNA genes, and two pseudogenes. (e-kjpt.org)
  • A reference phylogeny was reconstructed with 78 chloroplast genes for molecular identification and possible applications. (dbgi.org)
  • The availability of the genome sequence now opens the possibility of identifying genes involved in producing therapeutic molecules and to produce them in vitro (in the lab) according to CSIR. (livemint.com)
  • This will also facilitate identification of not-yet-identified genes involved in the synthesis of important secondary metabolites in this plant," said a press release issued by CSIR. (livemint.com)
  • The genome sequence and annotation of Basil provides new insights into the function of genes and the medicinal nature of the metabolites synthesized in this plant. (livemint.com)
  • Identification and analysis of unitary loss of long-established protein-coding genes in Poaceae shows evidences for biased gene loss and putatively functional transcription of relics. (myworldcup2006.com)
  • This quantity isn't traditionally needed in standard identification but can be critical when looking to sequence a whole genome. (nutraceuticalsworld.com)
  • With the higher throughput of next-generation sequencing, we are able to sequence chloroplast genomes or even entire genomes. (nutraceuticalsworld.com)
  • Complete Chloroplast Genome Sequence of a White Spruce ( Picea glauca , Genotype WS77111) from Eastern Canada. (gc.ca)
  • Here, I present the genome sequence of the L. fermentum LAB1 strain, its annotation, and phylogenetic features. (bvsalud.org)
  • Determination of average nucleotide identity (ANI) of the genome sequence revealed 99.37% identity to that of the type strain ATCC 14931. (bvsalud.org)
  • Its 16S rRNA gene sequence extracted from the genome sequence showed close phylogenetic association with several L. fermentum strains. (bvsalud.org)
  • The genome sequence is expected to provide useful insights with regard to the phenotypic, metabolic and beneficial aspects of this lactic acid bacterium. (bvsalud.org)
  • In contrast to the well-delimitated species boundary inferred from the nuclear ITS sequence variations, three of the four species are non-monophyletic in the plastome trees, which is consistent with previous studies based on a few chloroplast markers. (biomedcentral.com)
  • Previous study failed to discern the four Ostrya species based on four chloroplast (cp) fragments ( rps16 , trnG (UCC) intron, trnH-psbA , and trnL-trnF ) while the ITS sequence variations did [ 5 ]. (biomedcentral.com)
  • Variation in the chloroplast DNA sequence is useful for plant phylogenetic studies. (geneticsmr.com)
  • We found that these chloroplast genomes were conserved with respect to genome size, structure, and gene content and showed low sequence divergence. (cd-genomics.com)
  • The complete chloroplast genome (cp genome) sequence of Clematis calcicola J. S. Kim (Ranunculaceae) is 159,655 bp in length. (e-kjpt.org)
  • The complete cp genome sequence of C. calcicola reported here provides important information for future phylogenetic and evolutionary studies of Ranunculaceae. (e-kjpt.org)
  • Draft Genome Sequence of the Symbiotic Frankia sp. (scienceopen.com)
  • Here, we report the 5.3-Mbp draft genome sequence of Frankia sp. (scienceopen.com)
  • The system, software and consumables will initially be sold to research organizations, pharmaceutical companies and diagnostic companies that will sequence large regions of genomic DNA, including whole genomes, at costs several orders of magnitude below current levels. (scienceopen.com)
  • The complete chloroplast genome sequence of Garcinia oblongifolia (Clusiaceae). (myworldcup2006.com)
  • FDA is spearheading an international effort to build a network of laboratories that can sequence the genomes of foodborne pathogens and then upload the genomic sequence of the pathogen and the geographic location from which the pathogen was gathered into a publicly accessible database. (texas.gov)
  • Chloroplast Genome Characteristics of Endangered Relict Plant Tetraena mongolica in the Arid Region of Northwest China [J]. Bulletin of Botanical Research, 2019, 39(5): 653-663. (nefu.edu.cn)
  • Phylogenetic relationship reconstruction using whole chloroplast genome sequences agreed with previous work based on morphology. (umn.edu)
  • Species identification of an unknown sample is achieved by comparing its DNA barcode against reference sequences from authenticated specimens of that species. (nature.com)
  • To identify conserved regions that can be used to design primers and amplify variable sequences for use in plant phylogenetic studies, the complete chloroplast genomic sequences of six plant species (including Oryza sativa , Arabidopsis thaliana , Glycine max , Lotus japonicus , Medicago truncatula , and Phaseolus vulgaris ), searched from the taxonomy database of NCBI were investigated. (geneticsmr.com)
  • To infer the phylogenetic relationships among Clematis species, the complete chloroplast genome sequences of 20 Clematis species were downloaded from GenBank ( https://www.ncbi.nlm.nih.gov/genbank/ ). (e-kjpt.org)
  • The research effort focused on finding sequences in the tomato genome responsible for how the fruits ripened. (theaggie.org)
  • Of these, 257 samples were identified to the species level and 504 samples to the family level using specimens and chloroplast sequences. (dbgi.org)
  • However, the division of labor between PEP and NEP during plastid development and in mature chloroplasts is unclear. (frontiersin.org)
  • We show here that PLASTID REDOX INSENSITIVE 2 (PRIN2) and CHLOROPLAST STEM-LOOP BINDING PROTEIN 41 kDa (CSP41b), two proteins identified in plastid nucleoid preparations, are essential for proper plant embryo development. (frontiersin.org)
  • It reveals functionally distinct plastid and chloroplast development stages, identifies processes occurring in each of them, and highlights our very limited knowledge of the earliest drivers of plastid biogenesis, while providing a basis for their future identification. (biomedcentral.com)
  • BiGG Models: A platform for integrating, standardizing, and sharing genome-scale models (2016) Nucleic Acids Research 44(D1):D515-D522. (ucsd.edu)
  • However, the evolution and structure of mitochondrial genomes (mitogenomes) remain unclear in the Rosaceae family. (biomedcentral.com)
  • For instance, identification of a specimen from a species-poor plant community naturally poses a far lesser challenge than one from a species-rich location or an island flora that has undergone extensive species radiation and/or hybridization 6 . (nature.com)
  • While the entire genome may not be necessary for identification, taking a deeper look into the genome can provide new information about the history of an organism or can even discover new similarities and differences among species. (nutraceuticalsworld.com)
  • The complete chloroplast (cp) genome data has become a promising strategy to determine geographical origin and understand species evolution for closely related plant species. (bvsalud.org)
  • Herein, we sequenced the complete cp genome of S. tetrandra from Zhejiang Province and conducted a comparative analysis within Stephania plants to reveal the structural variations, informative markers and phylogenetic relationship of Stephania species. (bvsalud.org)
  • In addition, five pairs of specific primers targeting the divergence regions were accordingly designed, which could be utilized as potential molecular markers for species identification, population genetic and phylogenetic analysis in Stephania species. (bvsalud.org)
  • Overall, we provided comprehensive and detailed information on the complete chloroplast genome and identified nucleotide diversity hotspots of Stephania species. (bvsalud.org)
  • However, studies based on limited informative sites of several chloroplast markers failed to resolve interspecific delimitation and relationships among the four Chinese species. (biomedcentral.com)
  • We assembled and annotated 33 complete chloroplast genomes (plastomes) of the four Chinese species, representing 17 populations across most of their geographical distributions. (biomedcentral.com)
  • In addition, the plastomes with more informative sites compared with a few chloroplast markers still failed to resolve the phylogenetic relationships of the four species, and further studies involving population genomic data may be needed to better understand their evolutionary histories. (biomedcentral.com)
  • Pathologic examination and whole-genome sequencing identified this species of algae, and susceptibility testing determined antimicrobial resistance patterns. (cdc.gov)
  • Genus and species were not identified by matrix-assisted laser desorption ionization/time-of-flight mass spectrometry and GEN III Microbial identification (Biolog, https://www.biolog.com ). (cdc.gov)
  • The five newly developed primer pairs are recommended as tools for use in the identification of plant species and in phylogenetic studies. (geneticsmr.com)
  • For the rapid and accurate identification of Dalbergia species and assessment of phylogenetic relationships, it would be highly desirable to develop more effective DNA barcodes for these species. (cd-genomics.com)
  • In this study, we sequenced and compared the chloroplast genomes of nine species of Dalbergia. (cd-genomics.com)
  • A phylogenetic analysis based on the cp genome of 19 taxa showed high similarity between our cp genome and data published for C. calcicola , which is recognized as a species endemic to the Korean Peninsula. (e-kjpt.org)
  • The complete chloroplast genome of C. calcicola , and the phylogenetic analysis results reported in the present study provide information on the relationship between C. calcicola and related species, which may be a valuable resource for future studies on this species. (e-kjpt.org)
  • However, only a limited number of plant species have had their whole genome sequenced, and the limited taxon information of these species has further restrained the utility of these data. (dbgi.org)
  • We generated 54 Tb sequencing data in total, which resulted in an average sequencing depth of 60× for these species, as estimated by the genome size. (dbgi.org)
  • The complete chloroplast genome of a critically endangered tree species, Hopea reticulata (Dipterocarpaceae). (myworldcup2006.com)
  • The B. carinata (Ethiopian mustard, BBCC, 2n = 4X = 34) is an important species that contains 17 sets of chromosomes with a genome size of 1.087 Gb. (nature.com)
  • This is the first book on the genome of Salvia miltiorrhiza, summarizing the research advances in the molecular mapping, whole genome sequencing, chloroplast and mitochondria genomes, epigenetics, transcriptomics and functional genomics of this emerging model plant with great economic and medicinal value. (stanford.edu)
  • Chloroplasts, like mitochondria, evolved from free-living prokaryotic organisms that entered the eukaryotic cell through endosymbiosis. (frontiersin.org)
  • This process involves arranging all of an organism's chromosomal DNA as well as DNA contained in the mitochondria and, for plants, in the chloroplast. (texas.gov)
  • Originally, Sanger sequencing brought identification of a single, dominant signal. (nutraceuticalsworld.com)
  • Chloroplast genomes have been found to be helpful for the identification of Echinacea in a study conducted by the FDA which may indicate future developments in the sequencing space. (nutraceuticalsworld.com)
  • Long-read sequencing technologies are advancing rapidly, and have already shown significant improvements in costs, portability, and accuracy, which should eventually make whole genome sequencing a more financially feasible option. (nutraceuticalsworld.com)
  • 4.2 The Sequencing and Assembly of S. miltiorrhiza Genome 4.2.1 Sequencing of the S. miltiorrhiza 99-3 Genome (Xu et al. (stanford.edu)
  • Genome paired-end sequencing was performed at Macrogen Inc. on an Illumina platform (Illumina Inc., San Diego, CA, USA) based on 301 bp read size. (e-kjpt.org)
  • Genome sequencing has been widely used in plant research to construct reference genomes and elucidate evolutionary insights. (dbgi.org)
  • In this study, we established a large dataset of vascular plants' genomes, with both the high-depth whole genome sequencing data and the voucher specimens, making it valuable dataset for plant genome researches and applications. (dbgi.org)
  • Complete chloroplast genome of Stephania tetrandra (Menispermaceae) from Zhejiang Province: insights into molecular structures, comparative genome analysis, mutational hotspots and phylogenetic relationships. (bvsalud.org)
  • This study characterizes the variations in gene content, genome size, rearrangement rate, and the impact of domestication in Rosaceae mitogenomes and provides insights into their structural variation patterns and phylogenetic relationships. (biomedcentral.com)
  • To address these questions we have used a population genomic dataset of 12 strains of O.tauri that have been sequenced by Joint Genome Institute at more 100-fold coverage. (europa.eu)
  • are very small (~1m) unicellular autotrophic cells, with a very small nuclear genome (~13MB) [3,4], containing a single chloroplast and mitochondrion. (europa.eu)
  • Each mitochondrion has its own genome, which is usually uniparentally inherited [ 2 ]. (biomedcentral.com)
  • The nuclear genome of basil is the smallest in the family Lamiaceae, while the chloroplast genome is the smallest in the order Lamiales. (livemint.com)
  • Chloroplast Genome Characterization and Identification of Genetic Relationship of Relict Endangered Plant Amygdalus nana [J]. Bulletin of Botanical Research, 2020, 40(5): 686-695. (nefu.edu.cn)
  • A first draft genome of the Sugarcane borer, Diatraea saccharalis. (usda.gov)
  • Chloroplast biogenesis and function is essential for proper plant embryo and seed development but the molecular mechanisms underlying the role of plastids during embryogenesis are poorly understood. (frontiersin.org)
  • The developmental gradient in monocot leaves has been exploited to uncover leaf developmental gene expression programs and chloroplast biogenesis processes. (biomedcentral.com)
  • It is not dissimilar to the gradient of proliferation and differentiation along developing roots [ 2 ] but, in contrast, it provides a unique opportunity to study chloroplast biogenesis and differentiation. (biomedcentral.com)
  • Chloroplast biogenesis involves a multiplicity of processes. (biomedcentral.com)
  • In an effort to develop identification methods for fescues, we used flow cytometry to estimate genome size and ploidy level and sequenced the chloroplast genome of all five taxa. (umn.edu)
  • Patterns of genome-wide variation, population differentiation and SNP discovery of the red banded stink bug (Piezodorus guildinii). (usda.gov)
  • Comparative genomics suggested unique repeat signatures for each fine fescue taxon that could potentially be used for marker development for taxon identification. (umn.edu)
  • The comparative genome-wide analysis and mode of evolution of KCS and ELO gene families have not been investigated in tetraploid Brassica carinata and its diploid progenitors. (nature.com)
  • Complete chloroplast genome of Camellia japonica genome structures, comparative and phylogenetic analysis. (uinsgd.ac.id)
  • Phylogenetic analyses based on whole chloroplast genome data provided the best resolution of Dalbergia , and phylogenetic analysis of the Fabaceae showed that Dalbergia was sister to Arachis. (cd-genomics.com)
  • A Pyrus -specific deletion (DEL-D) in selective sweeps was identified based on the assembled genomes and population data. (biomedcentral.com)
  • However, the relationship between the two is barely understood, which limits the value of transcriptome data to understand the process of chloroplast development. (biomedcentral.com)
  • The twin-arginine translocation (Tat) pathway is another protein transport system that transports folded proteins in bacteria, archaea, and chloroplasts. (genome.jp)
  • Modification of the C. reinhardtii chloroplast genome is routine and many single proteins have been synthesised in the organelle. (ucl.ac.uk)
  • Based on comparison of chloroplast genomes, we identified a set of highly variable markers that can be developed as specific DNA barcodes. (cd-genomics.com)
  • The presented results help to enlarge the repertoire of molecular tools and know-how available for chloroplast engineering in C. reinhardtii. (ucl.ac.uk)
  • In this study, we assembled 34 Rosaceae mitogenomes and characterized genome variation, rearrangement rate, and selection signal variation within these mitogenomes. (biomedcentral.com)
  • A chromosome- scale genome and transcriptomic analysis of the endangered tropical tree Vatica mangachapoi (Dipterocarpaceae). (myworldcup2006.com)
  • Analysis of the genome revealed the presence of high numbers of secondary metabolic biosynthetic gene clusters. (scienceopen.com)
  • The algal chloroplast is a unique sub-cellular compartment with its own genetic system, which houses core metabolic pathways for carbohydrates, fatty acids, terpenoids and tetrapyrroles. (ucl.ac.uk)
  • Thus, the challenge is to increase the complexity of chloroplast engineering to allow sophisticated metabolic engineering. (ucl.ac.uk)
  • Taking advantage of the developmental gradient in the bread wheat leaf, we provide a simultaneous quantitative analysis for the development of mesophyll cells and of chloroplasts as a cellular compartment. (biomedcentral.com)
  • Our analysis generates both the first wheat leaf transcriptional map and one of the most comprehensive descriptions to date of the developmental history of chloroplasts in higher plants. (biomedcentral.com)
  • Genome skimming and supplementary barcodes could improve diagnostic power but would slow new barcode acquisition. (nature.com)
  • Therefore, this review aims to summarize recent studies on the geographic distribution, taxonomic identification, pharmacology, clinical applications and safety issues related to rhubarb and provide insights into the further development and application of rhubarb in the future. (biomedcentral.com)
  • Using a network reconstruction algorithm, we predict that known chloroplast gene expression regulators are differentially involved across those developmental stages. (biomedcentral.com)
  • both identification and classification of fine fescues remain challenging. (umn.edu)
  • Genotype Classification and Fluorescence Visual Identification of the Rhizoma Paridis of Paris Polyphylla Var. (researchsquare.com)
  • Identification, genealogical structure and population genetics of S-alleles in Malus sieversii , the wild ancestor of domesticated apple. (myworldcup2006.com)
  • With the increasing number of cultivars, accurate identification becomes fundamental to selecting the right cultivar for grafting, industrial processing or development of new cultivars. (kib.ac.cn)
  • This study proposed for the first time the existence of different genotypes on the legal basis of Rhizoma Paridis, and provided a model for the accurate identification of different genotypes. (researchsquare.com)
  • In the present study, the complete chloroplast genome (chloroplast genome) of C. calcicola is reported for the first time, to the best of our knowledge. (e-kjpt.org)
  • To ensure proper identification of cultivars and understand the genetic structure of wild and cultivated material, we genotyped 362 cultivated and wild individuals of walnut trees from southwest China (with two additional populations from Xinjiang, plus three cultivars from Canada, France and Belgium) using 36 polymorphic microsatellite loci. (kib.ac.cn)
  • both coding regions on the chloroplast genome. (nature.com)
  • The cp genome of S. tetrandra voucher ZJ was 157,725 bp, consisting of a large single copy region (89,468 bp), a small single copy region (19,685 bp) and a pair of inverted repeat regions (24,286 bp each). (bvsalud.org)