• Bacterial growth detection (or absence) is based on carbohydrate metabolism ( 11 ). (cdc.gov)
  • Acid formation associated with carbohydrate metabolism in Enterobacteriaceae can be observed through the color change of a pH indicator. (cdc.gov)
  • IL-1 beta functions in a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. (rndsystems.com)
  • The depletion of DOM over time was strongly correlated with increases in expression of many genes associated with heterotrophy (e.g., amino acid, fatty acid, and carbohydrate metabolism) belonging to a Hydrogenophaga strain that accounted for a relatively large percentage (~8%) of the metatranscriptome. (frontiersin.org)
  • The blocks of A. oryzae -specific sequence are enriched for genes involved in metabolism, particularly those for the synthesis of secondary metabolites. (nature.com)
  • Gene ontology (GO) and pathway enrichment analysis demonstrated that the DEGs were mainly implicated in energy metabolism, metabolism of terpenoids and polyketides, and carbohydrate metabolism related pathways. (biomedcentral.com)
  • Carbohydrate metabolism, inborn errors refer to genetic disorders that affect the body's ability to properly process carbohydrates, leading to various metabolic disorders. (lookformedical.com)
  • A slightly higher fraction of genes belonging to the categories amino acid metabolism (T. velox 11.8%, T. acidaminovorans 11.4%), carbohydrate metabolism (T. velox 5. (phosphorylase-signal.com)
  • Non-specific binding of primary antibodies may occur due to either binding of antibody to Fc receptors or non-specific interactions with cellular proteins, lipids, or carbohydrates. (genetex.com)
  • The four types most important to human structure and function are carbohydrates, lipids, proteins, and nucleotides. (chemicalindustrynews.com)
  • They range from small molecules such as primary and secondary metabolites and hormones to large macromolecules like proteins, nucleic acids, carbohydrates, lipids etc. (sarthaks.com)
  • Glycoprotein hormones are heterodimers consisting of a common alpha subunit and a unique beta subunit (this protein) which confers biological specificity. (wikipedia.org)
  • We identified a lipA gene in the genome sequence of Acremonium alcalophilum , encoding a protein with a predicted lipase domain with weak sequence identity to characterized enzymes. (biomedcentral.com)
  • We show that one of these, which we name SYMRKL1, encodes a protein with an ectodomain predicted to be nearly identical to that of SYMRK and is required for normal infection thread formation. (bvsalud.org)
  • The mouse MMR protein is synthesized as a 1456 amino acid (aa) precursor that contains a 19 aa signal sequence, a 1369 aa extracellular region, a 21 aa transmembrane segment and a 47 aa cytoplasmic domain (4). (rndsystems.com)
  • The MUC1 protein, also known as polymorphic epithelial mucin or epithelial membrane antigen, has a large extracellular region, a transmembrane sequence, and a cytosolic domain. (medscape.com)
  • Several mutations in the N terminus of the G-protein-coupled receptor rhodopsin disrupt NXS/T consensus sequences for N-linked glycosylation (located at N2 and N15) and cause sector retinitis pigmentosa in which the inferior retina preferentially degenerates. (jneurosci.org)
  • Whereas the trisaccharidic head group of Gb 3 defines the specificity of Shiga toxin binding, the lipophilic part composed of sphingosine and different fatty acids is suggested to determine its localization within membranes impacting membrane organisation and protein binding eventually leading to protein internalisation. (springer.com)
  • Although existing protin-centric informatics resources provide the biomedical research community with well-curated compendia of protein sequence and structure, these resources lack formal ontological representations of the relationships among the proteins themselves. (biomedcentral.com)
  • Although numerous methods of indirect bioconjugate preparation have been described, a significant number of those reported in the literature have been prepared by exploiting the Avidin-Biotin system, in which, the binding specificity of the protein Avidin (purified from egg white), or Streptavidin (purified from the bacterium Streptomyces avidinii), toward the cofactor Biotin (vitamin H) is utilized to bridge an Avidin conjugated macromolecule with a biotinylated macromolecule. (justia.com)
  • With the exploding landscape of available protein sequences it has become possible to model the structures of individual proteins with increasing accuracy from sequence information alone and these models could soon be used as building blocs to assemble larger macromolecular complexes, using computation methods and additional experimental clues. (bioexcel.eu)
  • If a batch of a single protein is treated with enzymes that clip the sequence wherever a particular type of peptide bond exists, the resulting bits can be separated by electrophoresis. (profmcdarby.com)
  • These proteins were found to be identical to the acidic and basic FGFs described by Gospodarowicz and coworkers. (beauty104.com.tw)
  • It belongs to a family of proteins called collectins, which consists of collagenous region and a carbohydrate recognition domain (Taylor et al. (springeropen.com)
  • The chemical or biochemical addition of carbohydrate or glycosyl groups to other chemicals, especially peptides or proteins. (lookformedical.com)
  • This process can be used to isolate proteins for further analysis, as well as to find differences in proteins in two near-identical systems, such as closely-related organisms. (profmcdarby.com)
  • Each bit can be analyzed in a similar way, and rough sequences can be built, as well as isolating critical differences between very similar proteins. (profmcdarby.com)
  • Because isotype control antibodies have no relevant specificity, they enable the researcher to distinguish non-specific "background" binding from antigen-specific antibody binding. (genetex.com)
  • Part of the antibody molecule that is identical between all antibodies of the same isotype, comprised of one or more constant domains. (stratech.co.uk)
  • The detection level accomplished in this study should help not only to further characterize anti-LOS antibodies in blood and body fluids but also to analyze carbohydrate structures that are recognized by them. (go.jp)
  • Although utilized extensively, several limitations are known to be associated with the Avidin-Biotin system, which include nonspecific binding generally attributed to the basicity of the Avidin molecule, nonspecific binding attributed to the presence of carbohydrate residues on the Avidin molecule, and background interference associated with the presence of endogenous Biotin, which is ubiquitous in both eukaryotic and prokaryotic cells. (justia.com)
  • Enzymes that catalyze the transfer of mannose from a nucleoside diphosphate mannose to an acceptor molecule which is frequently another carbohydrate. (lookformedical.com)
  • Cross-reactivity is sometimes desirable when one wants general binding to a related group of antigens or when attempting cross-species labeling when the antigen epitope sequence is not highly conserved during evolution. (sigmaaldrich.com)
  • Therefore, the primary antibody used should be matched to an isotype control of identical species and isotype (including both heavy and light chains). (genetex.com)
  • Antibody VIM2 reacts with a carbohydrate structure expressed by myeloid cells. (nordicmubio.com)
  • Ultimately these factors define the specificity of the antibody, that is, the likelihood that the particular antibody is binding to a precise antigen epitope. (sigmaaldrich.com)
  • This can be caused either by low avidity or specificity of the antibody or by multiple distinct antigens having identical or very similar epitopes. (sigmaaldrich.com)
  • Short sequences of amino acids found on the antibody variable region that collectively form the paratope. (stratech.co.uk)
  • This takes advantage of the specificity of antibody molecules produced by mammals. (profmcdarby.com)
  • For this purpose, a mAb-concentration range is selected to include both the saturation point (i.e. the mAb dilution expected to bind all epitopes on the target cell) and the detection threshold (i.e. the mAb dilution expected to represent the least amount of mAb needed to detect an identical percentage of cells). (nordicmubio.com)
  • 5] These carbohydrate epitopes may be antigenically different than those in normal breast cells. (medscape.com)
  • Immunochemical techniques capitalize upon the extreme specificity, at the molecular level, of each immunoglobulin for its antigen, even in the presence of high levels of contaminating molecules. (sigmaaldrich.com)
  • The CA 15-3 antigen (also known as MUC1, from which it is derived) represents sequences of mucins that are often overexpressed in malignant glandular cells, such as breast cancer. (medscape.com)
  • Elevated cell-surface expression of truncated carbohydrate structures such as Tn antigen (GalNAcα-Ser/Thr) is frequently observed during tumor progression. (oncotarget.com)
  • The genome sequence of Acremonium alcalophilum has revealed a large number of genes encoding biomass-degrading enzymes. (biomedcentral.com)
  • however, as evidenced by genome sequence analysis, the organism lacks common enzymes required for the de novo synthesis of precursors of PGN, which rationalizes its MurNAc auxotrophy. (karger.com)
  • Sensitivity and specificity were 99.3% and 95.4%, respectively, compared with the standard broth microdilution method. (cdc.gov)
  • In conclution, DNA amplification is a rapid, reliable and accurate method with a high degree of sensitivity and specificity for the detection of Mycobacterium tuberculosis DNA sequences and it can replace the conventional culture method in the diagnosis of extra pulmonary tuberculosis and tuberculosis meningitis except in the situation when antibiotic sensitivity results are required. (who.int)
  • Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. (lookformedical.com)
  • The (Z)-allene-ene-yne 10, a precursor to a reactive α,3-dehydrotoluene biradical, was covalently linked to a minor groove binding element, and the resultant conjugate (9) was shown to bind and cleave DNA with sequence selectivity. (caltech.edu)
  • Tumors express embryonic carbohydrate antigens called tumor-associated carbohydrate antigens (TACA). (silverchair.com)
  • The signaling complex at the cell surface is believed to be a ternary complex formed between two identical FGF ligands, two identical FGFR subunits and either one or two heparan sulfate chains. (beauty104.com.tw)
  • While this first joint experiment focused primarily on complexes comprising identical subunits, the experiment demonstrated beyond any doubt that integrations of different modeling techniques with information from various sources will the prevailing approach in the future. (bioexcel.eu)
  • Here we describe the deployment of new sequencing technology to extensively sample eight genomes from the Yersinia genus of the family Enterobacteriaceae. (biomedcentral.com)
  • In bacterial isolates of prisoners and ex-prisoners from the general population, there were 2 strains, which had identical banding patterns, while there were clear similarities between several isolates. (who.int)
  • Further insights on the roles of CD8 + specificities and TCR avidity of naturally arising tumor-specific T cells, where both high and low avidity T cells recognizing the same peptide-major histocompatibility complex (pMHC) coexist in the same tumor, are crucial for understanding T cell exhaustion and resistance to PD-1 immunotherapy. (bmj.com)
  • It binds to carbohydrates on the surface of mannose-rich pathogens and mediates clearing by phagocytosis or complement activation (Nepomuceno et al. (springeropen.com)
  • The activity of Endo F2 is identical on biantennary structures with and without core fucosylation. (neb.com)
  • The assembly included transcript sequences from both the coral host and its symbionts, which are related to the thermotolerant C3-Gulf ITS2 type Symbiodinium . (nature.com)
  • The results of cDNA cloning, peptide mapping, and amino acid sequencing of PPAE revealed that PPAE is synthesized as prepro-PPAE with 441 amino acid residues and is activated from pro-PPAE by cleavage of a peptide bond between Lys152 and Ile153. (lookformedical.com)
  • Examination of direct smears for acid fast bacilli is the widely used method for the detection of mycobacteria, but lacks the desired specificity and relatively large number of bacteria is needed for detection. (who.int)
  • The derived amino acid sequence of Ov-phy-1 encoded a peptide that was most similar to the two Caenorhabditis elegans prolyl 4-hydroxylase homologues and to the isoform II enzymes of vertebrates. (embl.de)
  • Recently, the genome of A. alcalophilum was sequenced ( http://genome.jgi-psf.org/Acral2/Acral2.home.html ), providing a rich genetic resource for the identification of enzymes that potentially function under alkaline conditions. (biomedcentral.com)
  • Think about the stages of respiration and photosynthesis - for almost every conversion of relatively simple carbohydrates, there are enzymes, coenzymes, and carriers involved - mostly huge, intricate molecules whose activity depends upon subtle differences in sequences and conformation. (profmcdarby.com)
  • While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. (rndsystems.com)
  • These sugars are thought not only to contribute to the structural conformation and stability of the Fc domain, but also mediate receptor binding through carbohydrate-carbohydrate interactions. (stratech.co.uk)
  • New DNA sequencing technologies have enabled detailed comparative genomic analyses of entire genera of bacterial pathogens. (biomedcentral.com)
  • That the distances relating the number of identical base pairs to total HSP length (formula 2) behave differently indicates that the genomic similarities between T. velox, T. acidaminovorans and A. paucivorans are strongly restricted to more conserved sequences, a kind of saturation phenomenon [42]. (phosphorylase-signal.com)
  • It is proposed that the species that determines the sequence specificity of DNA cleavage is thecumulene intermediate. (caltech.edu)
  • Prior to this study, three species of the enterobacterial genus Yersinia that cause invasive human diseases ( Yersinia pestis , Yersinia pseudotuberculosis , and Yersinia enterocolitica ) had been sequenced. (biomedcentral.com)
  • Tall fescue is also a hexaploid outcrossing species with a large genome size [ 8 ], and up to now its genome has not been sequenced. (biomedcentral.com)
  • Its extracellular region is composed of an N-terminal cysteine-rich domain, followed by a single fibronectin type II repeat, and eight C-type lectin carbohydrate recognition domains (CRD) (3‑5). (rndsystems.com)
  • Mouse to human, the extracellular region is 82% aa identical. (rndsystems.com)
  • The title compound, (4S,5S,8R,10Z)-2,2,5,6,8,10- hexamethyl-4-phenyl-l,3-dioxa-6-aza-2-silacycloundec- 10-en-7-one, crystallizes with two virtually identical molecules in the asymmetric unit, each with the same absolute configuration. (caltech.edu)
  • Several of the CRDs participate in the Ca 2+ -dependent recognition of carbohydrates showing a preference for branched sugars with terminal mannose, fucose or N‑acetylglucosamine (7). (rndsystems.com)
  • The 17 kDa molecular weight mature human IL-1 beta shares 96% aa sequence identity with rhesus and 67%-78% with canine, cotton rat, equine, feline, mouse, porcine, and rat IL-1 beta. (rndsystems.com)
  • To better understand the molecular mechanisms underlying its biology and ecology, we generated a reference transcriptome for H. coerulea using next-generation sequencing. (nature.com)
  • Unusually, the predicted lipase displays ≈ 30% amino acid sequence identity to both feruloyl esterase and lipase of Aspergillus niger . (biomedcentral.com)
  • The genome of Aspergillus oryzae , a fungus important for the production of traditional fermented foods and beverages in Japan, has been sequenced. (nature.com)
  • The results obtained here indicate that the binding of IgG in NHS to the LOS and LPS samples is probably due to their carbohydrate moieties. (go.jp)
  • These data suggest that small carbohydrate moieties may "fit" in the variable region of the TcR. (silverchair.com)
  • Rapid high-quality draft sequencing was used successfully to compare pathogenic and non-pathogenic members of the Yersinia genus. (biomedcentral.com)
  • In equids the beta subunit polypeptides of luteinizing hormone and chorionic gonadotropin are identical in sequence, differing only in their carbohydrate side-chains, and are the product of a single gene. (wikipedia.org)
  • Polysaccharides are complex carbohydrates composed of long chains of monosaccharide units, with important functions in the body including energy storage, structural support, and immune response. (lookformedical.com)
  • 1989 ). MBL consists of multimers of an identical polypeptide chain of 32 kDa. (springeropen.com)
  • 1998 ). The MBL2 gene is composed of 7461 bases and lies between the regions 52765380 to 52772841 bp of chromosome no. 10 (NCBI reference sequence number NC_000010.11). (springeropen.com)
  • It is determined that while there is evidence to support the hypothesis that the carbohydrate amino group of 1 participates in the thiol activation of 1 in organic solvents, no conclusions may be drawn at this time concerning its role in the corresponding reaction in water in the presence of DNA. (caltech.edu)
  • Expressed sequence tag (EST) analysis and developmental polymerase chain reaction (PCR) studies demonstrated that Ov-phy-1 was expressed in L3 and adult parasites. (embl.de)
  • Key features of this sequence include the coupling of the enol triflate 11 and the arylboronic acid 10 (90%), the thermal deprotection/internal amidation of the coupling product 18 (84%), the use of 2-chloropyridine as an economical alternative to 2,6-di-tert-butylpyridine to promote the reaction of the quinolone 9 and triflic anhydride (85%), the highly stereoselective addition of the (Z)-enediyne 31 to the quinoline 61 (89%), intramolecular acetylide addition within the acetylenic ketone 66 (94%), and oxidation of the phenol 76 with iodosobenzene to afford the quinone imine precursor 77 in 89% yield. (caltech.edu)
  • New and existing data pertaining to the potential role of DNA as a catalyst in the thiol activation reaction and to the potential participation of the carbohydrate amino group of 1 in that reaction is evaluated. (caltech.edu)
  • 95% of the carbohydrate from 10 µg Porcine Fibrinogen in 1 hour at 37°C in a total reaction volume of 10 µl. (neb.com)
  • The recent development of second generation sequencing platforms (reviewed by Mardis [ 7 , 8 ] and Shendure [ 7 , 8 ]) offers an opportunity to change the direction of microbial genomics, enabling the rapid genome sequencing of large numbers of strains of both pathogenic and non-pathogenic strains. (biomedcentral.com)
  • Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product. (genetex.com)
  • Together with other sialylated and fucosylated polylactosamines the carbohydrate structure recognized by VIM2 may play a critical role on the adhesion of granulocytes and monocytes to endothelium and platelets during inflammation and clotting. (nordicmubio.com)
  • One can define several major GSL series based on their internal core carbohydrate structure. (springer.com)
  • Of the 20 strains isolated from prisoners, none of the strains displayed identical fingerprints. (who.int)
  • More than 68 percent had less than 5 copies of the IS sequence suggesting that our local M.tuberculosis strains have a fewer number of copies compared to data shown in most countries. (who.int)
  • RNA sequencing data have been deposited in the Gene Expression Omnibus (GEO) under accession number GSE221590. (bmj.com)
  • These results are in line with a previously reported wet-lab DDH value of 15% (��1) [1] As expected, those distances relating HSP coverage (formula 1) and number of identical base pairs within HSPs to total genome length (formula 3) are higher between the T. velox and T. acidaminovorans than between T. velox and A. paucivorans. (phosphorylase-signal.com)
  • Genetic variants at PSMD3 interact with dietary fat and carbohydrate to modulate insulin resistance. (nih.gov)
  • From the general population 5 sets of identical banding patterns were observed. (who.int)
  • These innovations allow for the coupling of a uridine-derived aldehyde fragment with a performed trehalose-linked disaccharide allylic alcohol to form the carbohydrate core (1) of the natural product in a highly covergent manner. (caltech.edu)
  • in most carbohydrates, hydrogen and oxygen are found in the same two-to-one relative proportions they have in water. (chemicalindustrynews.com)
  • Table 4 Robustness study of lornoxicam and thiocolchicoside (n = 3) Specificity The method was found to be specific as no interfering spots were seen when Rf values of the standard and sample were compared. (phosphorylase-signal.com)
  • Syntenic analysis of the three aspergilli revealed the presence of syntenic blocks and A. oryzae -specific blocks of sequence (lacking synteny with the two other aspergilli) in a mosaic manner throughout the A. oryzae genome ( Fig. 1 ). (nature.com)
  • This suggests that the genome size differences are largely due to sequence acquisition in A. oryzae . (nature.com)
  • Although these factors possess remarkably similar sequence homology, they do not bind FGFRs and are involved in intracellular processes unrelated to the FGFs (Olsen et al. (beauty104.com.tw)
  • Thus, the increase in genome size seems to be due to an A. oryzae lineage-specific acquisition of sequence, rather than loss of sequence in A. nidulans and A. fumigatus . (nature.com)
  • The fine specificity of the TF-specific CTL repertoire indicates that the TcR recognize the glycosylated amino acid residue together with TF in a conventional major histocompatibility complex class I-restricted fashion. (silverchair.com)