• Enzymatic
  • Binding of cholesterol results in an enzymatic conformational change and a subsequent induced fit of the active site around the cholesterol molecule, anchoring the hydroxylation site (C-24, C-25) near the catalytic center of the enzyme (5.7Å from the iron core of the heme molecule to allow oxyferryl intermediates to perform the cholesterol hydroxylation). (wikipedia.org)
  • reaction
  • These observations suggested the participation of the cytochrome, P450, in the cholesterol 7α-hydroxylation reaction as the terminal electron acceptor and oxygen activator. (bl.uk)
  • There was no apparent correlation between P450 levels and the amount of 7a-hydroxycholesterol formed in the microsomal fraction and it is therefore unlikely that this haeinoprotein is the rate-controlling species in the 7a-hydroxylation reaction. (bl.uk)
  • The Milas hydroxylation is an organic reaction converting an alkene to a vicinal diol, and was developed by Nicholas A. Milas in the 1930s. (wikipedia.org)
  • The phenotypes for hydroxylation and acetylation can be predicted be newly developed methods based on mutation-specific amplification of DNA by the polymerase chain reaction (PCR), which also allow for identification of heterozygous carriers of one mutant allele. (springer.com)
  • Using ambient pressure X-ray photoelectron spectroscopy, we report that the affinity toward hydroxylation, coincident with strong adsorption energies calculated for dissociated water and hydroxyl groups, leads to strong H bonding that is favorable for wetting while detrimental to catalysis of the oxygen reduction reaction (ORR). (mit.edu)
  • An NIH shift is a chemical rearrangement where a hydrogen atom on an aromatic ring undergoes an intramolecular migration primarily during a hydroxylation reaction. (wikipedia.org)
  • vitro
  • Hydroxylation-deficient AKT mutants induced enhanced anchorage-independent growth in vitro and tumor formation in vivo compared with wild-type AKT, demonstrating that hydroxylation of AKT modulates oncogenic signaling. (aacrjournals.org)
  • As a first step in substantiating this hypothesis, we have developed and validated an in vitro propofol hydroxylation system, which incorporates a novel and highly sensitive 4-hydroxypropofol assay using HPLC with fluorescence detection. (aspetjournals.org)
  • phenol
  • One of the most interesting transformations yet to be efficiently developed from a practical point of view is the direct benzene hydroxylation toward phenol, for which a number of systems have been reported. (rsc.org)
  • We have previously reported 7 a copper-based catalyst containing a trispyrazolylborate type ligand 8 that induced the hydroxylation of benzene with high selectivity toward phenol ( Scheme 2 ), with H 2 O 2 as the oxidant and in the absence of acids. (rsc.org)
  • pathway
  • Whereas 4-hydroxylation constitutes the minor pathway in the liver, the greater proportion of CYP1B1 expression in extrahepatic tissues shifts the balance in favor of 4-OH-E2 formation. (wikipedia.org)
  • Taken together, these results indicate that hypoxia and defects in the VHL-EGLN pathway lead to hydroxylation-dependent hyperactivation of AKT, and suggest that targeting the AKT pathway may be effective in patients with VHL-deficient tumors as well as more generally in hypoxic tumors. (aacrjournals.org)
  • protein
  • Pseudomonas putida GPo1 alkane hydroxylase (AlkB) is an integral membrane protein that catalyses the hydroxylation of medium-chain alkanes (C 3 -C 12 ). (biochemj.org)
  • interaction
  • Moreover, two cancer-associated mutations, AKT1 G311D and AKT2 P127N , were identified that reduce AKT hydroxylation, disrupt the VHL-AKT interaction, and enhance AKT activation. (aacrjournals.org)
  • important
  • Site-directed mutagenesis combined with structural and functional analyses indicated that hydrophobic interactions and shielding effects against water are important factors in the hydroxylation of biphenyl by the o-xylene dioxygenase. (nih.gov)