• Enzymatic
  • Binding of cholesterol results in an enzymatic conformational change and a subsequent induced fit of the active site around the cholesterol molecule, anchoring the hydroxylation site (C-24, C-25) near the catalytic center of the enzyme (5.7Å from the iron core of the heme molecule to allow oxyferryl intermediates to perform the cholesterol hydroxylation). (wikipedia.org)
  • pathway
  • Taken together, these results indicate that hypoxia and defects in the VHL-EGLN pathway lead to hydroxylation-dependent hyperactivation of AKT, and suggest that targeting the AKT pathway may be effective in patients with VHL-deficient tumors as well as more generally in hypoxic tumors. (aacrjournals.org)
  • Whereas 4-hydroxylation constitutes the minor pathway in the liver, the greater proportion of CYP1B1 expression in extrahepatic tissues shifts the balance in favor of 4-OH-E2 formation. (wikipedia.org)
  • vitro
  • Hydroxylation-deficient AKT mutants induced enhanced anchorage-independent growth in vitro and tumor formation in vivo compared with wild-type AKT, demonstrating that hydroxylation of AKT modulates oncogenic signaling. (aacrjournals.org)
  • As a first step in substantiating this hypothesis, we have developed and validated an in vitro propofol hydroxylation system, which incorporates a novel and highly sensitive 4-hydroxypropofol assay using HPLC with fluorescence detection. (aspetjournals.org)
  • Reaction
  • The phenotypes for hydroxylation and acetylation can be predicted be newly developed methods based on mutation-specific amplification of DNA by the polymerase chain reaction (PCR), which also allow for identification of heterozygous carriers of one mutant allele. (springer.com)
  • An NIH shift is a chemical rearrangement where a hydrogen atom on an aromatic ring undergoes an intramolecular migration primarily during a hydroxylation reaction. (wikipedia.org)
  • phenol
  • One of the most interesting transformations yet to be efficiently developed from a practical point of view is the direct benzene hydroxylation toward phenol, for which a number of systems have been reported. (rsc.org)
  • We have previously reported 7 a copper-based catalyst containing a trispyrazolylborate type ligand 8 that induced the hydroxylation of benzene with high selectivity toward phenol ( Scheme 2 ), with H 2 O 2 as the oxidant and in the absence of acids. (rsc.org)
  • oxygen
  • Hydroxylation occurs at the γ-C atom, forming hydroxyproline (Hyp), which stabilizes the secondary structure of collagen due to the strong electronegative effects of oxygen. (wikipedia.org)
  • interaction
  • Moreover, two cancer-associated mutations, AKT1 G311D and AKT2 P127N , were identified that reduce AKT hydroxylation, disrupt the VHL-AKT interaction, and enhance AKT activation. (aacrjournals.org)
  • similar
  • This enzyme has also been found in insignificant quantities in the retina, where it serves a similar (but limited) function of catalyzing the hydroxylation of cholesterol for cholesterol-elimination from the eye. (wikipedia.org)