Protein EngineeringHydrolysisTissue EngineeringGenetic EngineeringDNA ShufflingModels, MolecularMetabolic EngineeringDirected Molecular EvolutionMolecular Sequence DataAmino Acid SequenceProtein ConformationKineticsMutagenesis, Site-DirectedProtein FoldingSubtilisinBiomedical EngineeringEscherichia coliInteinsSubstrate SpecificityProtein StabilityBinding SitesEnzyme StabilityProtein Structure, SecondaryProtein Structure, TertiaryBiotechnologyRecombinant ProteinsMutationProteinsMutagenesisCrystallography, X-RayTissue ScaffoldsSynthetic BiologyCatalysisProtein SplicingStructure-Activity RelationshipAmino Acid SubstitutionPeptide LibraryThermodynamicsBiophysical ProcessesProtein BindingMutant ProteinsBacterial ProteinsBacteriophage M13Biocompatible MaterialsPhosphoribosylglycinamide FormyltransferaseHydrogen-Ion ConcentrationBase SequenceCell Surface Display TechniquesTyrosine-tRNA LigaseRecombinant Fusion ProteinsProtein Denaturationalpha-AmylasesBioengineeringBacillusEnzymesCircular DichroismModels, ChemicalGenes, SyntheticCell EngineeringCatalytic DomainHydroxymethyl and Formyl TransferasesPeptidesAdenosine TriphosphateTemperatureSequence AlignmentChemical EngineeringBiosensing TechniquesSequence Homology, Amino AcidMolecular StructureCrystallizationDrug DesignCysteineTitrimetryMagnetic Resonance SpectroscopyTriose-Phosphate IsomeraseCloning, MolecularGeobacillus stearothermophilusGene LibraryGlycoside HydrolasesPlasmidsComputer SimulationElectrophoresis, Polyacrylamide GelDisulfidesNuclear Magnetic Resonance, BiomolecularMuramidaseDNA PrimersStereoisomerismSubtilisinsBioartificial OrgansProtein Structure, QuaternaryHydrophobic and Hydrophilic InteractionsAmino AcidsOxidation-ReductionEscherichia coli ProteinsDatabases, ProteinSolubilityAdenosine TriphosphatasesLigandsSaccharomyces cerevisiaeComputational Biology