Hybridization physical chromosome mapping. Medical search. Frequent questions

There are different techniques to visualize the gene location, including somatic cell hybridization, radiation hybridization and in situ hybridization. (wikipedia.org)
The three basic varieties of physical mapping are fluorescent in situ hybridization (FISH), restriction site mapping and sequencing by clones. (wikipedia.org)
Since their hierarchic structure is less condensed comparing to prometaphase and metaphase chromosomes, the standard in situ hybridization target, a high resolution of physical mapping could be produced. (wikipedia.org)
In the fall of 2011 Dr. Sharakhov's laboratory installed an Xmatrx Infinity system to physically map chromosomes of various invertebrate species using automated fluorescent in situ hybridization (FISH). (biogenex.com)
We had lots of physical markings of chromosomes to do," said Dr. Sharakhov whose project is now published in the Journal of Visualized Experiments (JoVE): George, P., Sharakhova, M. V., Sharakhov, I. V. High-throughput Physical Mapping of Chromosomes using Automated in situ Hybridization. (biogenex.com)
Here, we provide the first cytogenetic information on the species based on both conventional karyotyping and chromosomal mapping of 45S and 5S ribosomal genes through fluorescence in situ hybridization (FISH). (scielo.br)
You could use that material to build chromosome-specific libraries or chromosome-specific probes and use that for fluorescence in situ hybridization. (nih.gov)
So what we designed a fluorescence in situ hybridization procedure to detect the fusion gene. (nih.gov)
Physical mapping of ribosomal genes by Fluorescent Molecular in situ Hybridization. (tsukuba.ac.jp)
This cosmid was assigned to bovine Chromosome (Chr) 4 (BTA 4) by fluorescence in situ hybridization (FISH). (tamu.edu)
Genetic analysis included karyotyping, X inactivation studies, fluorescent in situ hybridization, microsatellite marker genotyping, and array comparative genomic hybridization. (biomedcentral.com)
Fluorescence in situ hybridization and BAL31 nuclease digestion demonstrate that the (TTAGGG)n sequence occurs at the extreme termini of the chromosomes and also at an interstitial site. (westminster.ac.uk)
18. What nucleic acid does in situ hybridization stain? (examyear.com)
19. When used in in situ hybridization, RNA probes are _______ to the sample's RNA. (examyear.com)
results can serve as an important argument in favour of the proposal to develop a special program for sequencing the genome chromosomes and for, To estimate the possibility of plant genome mapping using human genome probes, the probes fluorescent in situ hybridization (FISH) of human 18S-28S rDNA (clon 22F9 from the LA-13NCO1 library) was carried out on chromosomes of the spring barleyHordeum vulgareL. (centrocardiobogota.com)
Evaluation of F1 and BC1 hybrids of Lilium lancifolium × Asiatic hybrid 'Chianti' by morphological analysis and fluorescence in situ hybridization. (livedna.net)
Application of genomic in situ hybridization in horticultural science. (livedna.net)
A genome-wide in situ hybridization map of RNA-binding proteins reveals anatomically restricted expression in the developing mouse brain. (nih.gov)
Based on previous research, the identifications of 6VS, 3B and 7BL chromosomes (arms) sorted from ditelosomic and common wheat were performed through fluorescence in situ hybridization (FISH), primed in situ DNA labeling (C-PRINS) and PCR amplification methods respectively. (chinacrops.org)
C-PRINS combined the advantages of FISH and PCR, had potential for chromosomes identification, although the hybridization signals was instable and repetition was not so good at present, if combined this method with in situ hybridization in suspension, a new way for chromosome flow sorting might be set up. (chinacrops.org)

In order to improve cultivated wheat, genes and traits of agricultural importance need to be positioned accurately on chromosomes and closely linked markers identified to allow rapid selection in breeding programs. (usda.gov)
This is a pre-requistied to complete sequencing of this chromosomes and identifying the important genes that can be used to improve modern wheat cultivars. (usda.gov)
Both classes of ribosomal genes appeared to be spread out to multiple chromosomal locations, i.e. the 45S and 5S rDNA clusters were detected on six and seven chromosome pairs, respectively. (scielo.br)
We have contributed to the assembly process by mapping markers/genes to BACs and contigs using overgo hybridization. (kent.ac.uk)
Detailed mapping suggests that haploinsufficiency of one or more genes in Xp22.3, the distal 8.3 megabases (Mb) of the X chromosome, is responsible for a TS neurocognitive phenotype. (biomedcentral.com)
The genetic abnormality in TS is determined by the absence of one copy of genes on the X chromosome. (biomedcentral.com)
Although one X chromosome undergoes inactivation in normal females during early embryogenesis, about 15% of all X chromosome genes, mostly situated on the short arm (Xp), remain active to some degree on both X chromosomes [ 17 ]. (biomedcentral.com)
A total of 93 BAC clones were used to localize the chromosome complement of the species and 588 genes were annotated, thus almost reaching the 2.5% of the genome sequences. (metasystems-international.com)
In addition, chromosomes with a high number of linked genes that are conserved, even in distant species, were detected. (metasystems-international.com)
With the human DNA probe, signals were detected in the major sites of the ribosomal genes on chromosomes 5I (7H) and 6I (6H) only when the chromosome preparations were obtained using an optimized technique with obligatory pepsin treatment followed by hybridization. (centrocardiobogota.com)
The above-mentioned ab, of repetitive sequences in plant genomes (up to 99%), makes difﬁcult the application of this technique for, mapping individual plant genes [40]. (centrocardiobogota.com)
A team of IAE scientists determined the physical location of 242 tammar wallaby genes on their eight pairs of chromosomes. (edu.au)
They used a technique that fluorescently tags genes, enabling their location on a chromosome to be visualised. (edu.au)
Identifying genes on each chromosome is an active area of genetic research. (medlineplus.gov)
Because researchers use different approaches to predict the number of genes on each chromosome, the estimated number of genes varies. (medlineplus.gov)
Chromosome 1 likely contains 2,000 to 2,100 genes that provide instructions for making proteins. (medlineplus.gov)
Researchers have identified several possible tumor suppressor genes in the deleted region of chromosome 1, and more research is needed to understand what role these genes play in neuroblastoma development. (medlineplus.gov)
Any method used for determining the location of and relative distances between genes on a chromosome. (bvsalud.org)

Fluorescent in situ hybridisation (FISH) on metaphase chromosomes and on extended chromatin will be used to physically locate repetitive as well as single copy DNA sequences on conifer chromosomes and to integrate physical and genetic maps. (le.ac.uk)
More recently, the technique of comparative genomic hybridisation (CGH) using metaphase chromosomes has been applied to this clinical problem. (bmj.com)

In patients with normal karyotypes, the clinical features may still suggest appropriate regions to be screened for microscopic imbalance by fluorescent in situ hybridisation (FISH) analysis using locus specific probes. (bmj.com)

In this category, most mapping methods involve generating a somatic cell hybrid panel, which is able to map any human DNA sequences, the gene of interest[clarification needed], to specific chromosomes of animal cells, such as those of mice and hamsters. (wikipedia.org)
FISH mapping using interphase chromosome is a conventional in situ method to map DNA sequences from 50 to 500 kilobases, which are mainly syntenic DNA clones. (wikipedia.org)
a) PCR-based amplification of randomly selected bin mapped sequences (b) comparison with previously mapped ESTs and (c) comparison with a 7B genetic map developed in the present study. (usda.gov)
An examination of the content and distribution of repetitive DNA sequences to identify the location of such sequences within specific chromosomes and their conservation across species of the Pinaceae family. (le.ac.uk)
Characterisation and mapping of expressed sequence tags or EST's to serve as anchored sequences tagged sites (STS). (le.ac.uk)
Overgo hybridization provides a cost-effective, high throughput method for integration of physical maps, sequences and linkage maps for many species, including other avians. (kent.ac.uk)
Using a gene microarray containing ∼40 K NCBI UniGene EST clusters, PCR marker screening and BAC end sequences, we arranged 160 physical contigs (97 Mb or 35.3% of the chromosome arm) in a virtual order based on synteny with Brachypodium, rice and sorghum. (inrae.fr)
BAC end sequences and information from microarray hybridisation was used to anchor 3.8 Mbp of Illumina sequences from flow-sorted chromosome 1AS to BAC contigs. (inrae.fr)
In the present article, a review and new processed data have allowed to obtain a high-density BAC-based cytogenetic map of beside the analysis of the sequences of such BAC clones to achieve integrative data. (metasystems-international.com)
To better understand the karyotype organization in Melipona and the relationship among the subgenera, we mapped repetitive sequences and analyzed previously reported cytogenetic data with the aim to identify cytogenetic markers to be used for investigating the phylogenetic relationships and chromosome evolution in the genus. (karger.com)

A major technique to map such large DNA regions is high resolution FISH mapping, which could be achieved by the hybridization of probes to extended interphase chromosomes or artificially extended chromatin. (wikipedia.org)
CGH involves hybridizing reference and testing DNA samples onto chromosome or microarray slides with DNA probes representing all regions of the genome, then comparing their fluorescence intensity ratio. (ablogwithadifference.com)
Characterisation of Robertsonian translocations in cattle by in situ hybridisation using centromeric probes. (edu.au)
3- 16 However, these probes only reveal chromosome rearrangements located in the subtelomeric region. (bmj.com)
DNA from this somatic cell hybrid gave positive results in Southern blot hybridization analyses with probes for 4p16.2p15.1, D4S20, and 4q21q23, ADH3. (coriell.org)
The FISH approach could provide a visible and repetitive result and was suit for identifying purity of the sorted chromosomes, but it was time-consuming, complex and necessary for special probes. (chinacrops.org)

The physical map of 5DS was comprised of 164 contigs, of which 45 were organized into 21 supercontigs, covering 176 Mb with an N50 value of 2,173 kb. (biomedcentral.com)
Ordering of 80 contigs along the deletion bins of chromosome arm 5DS revealed small-scale breaks in syntenic blocks. (biomedcentral.com)
A total of 3,671 sequence contigs and scaffolds were mapped to deletion bins on wheat chromosome 7B providing a foundation for developing high-resolution integrated physical map for this chromosome. (usda.gov)
Chinese spring deletion lines, a total of 3,671 sequence contigs and scaffolds (~7.8 % of chromosome 7B physical length) were mapped into nine deletion bins. (usda.gov)
Validation of the bin mapping results suggested a high accuracy of the assignment of 7B sequence contigs and scaffolds to the 7B deletion bins. (usda.gov)
From the assembly of 25,918 high information content (HICF) fingerprints from a 1AS-specific BAC library, 715 physical contigs were produced that cover almost 99% of the estimated size of the chromosome arm. (inrae.fr)
A high-quality reference sequence for the Brassica napus genome is not yet available, thus studies of specific regions of the Brassica napus genome at sequence level have relied on the analysis of bacterial artificial chromosomes (BACs) or BAC contigs. (biomedcentral.com)

Dr. Sharakhov is focused on identifying the chromosomal physical map. (biogenex.com)
gene is mapped on the wheat chromosome 5B, but, neither obtaining a physical marker nor microdissec-, tion of this chromosomal region gave practical results, [56]. (centrocardiobogota.com)
After comparing to these three protocols, the PCR approach was the fastest with better repetition which adapted to determine rapidly constitute of chromosomal peaks on the univariate flow karyotype histogram, but there were no visible signals and the purity of sorted chromosomes could not be determined were the disadvantages of this approach. (chinacrops.org)
The following chromosomal conditions are associated with changes in the structure or number of copies of chromosome 1. (medlineplus.gov)
1q21.1 microdeletion is a chromosomal change in which a small piece of the long (q) arm of chromosome 1 is deleted in each cell. (medlineplus.gov)
[ 1 ] They described a child with midline fusion defects, and subsequent cytogenetic studies revealed a chromosomal deletion of the short arm of chromosome 4. (medscape.com)

The hybrid cell panel is produced by collecting hybrid cell lines containing human chromosomes, identified by polymerase chain reaction (PCR) screening with primers specific to the human sequence of interest as the hybridization probe. (wikipedia.org)
Efficient screening of bacterial artificial chromosome (BAC) libraries with polymerase chain reaction (PCR)-based markers is feasible provided that a multidimensional pooling strategy is implemented. (biomedcentral.com)

5 clones) that constitute ~95 % of the cumulative length of the map. (biomedcentral.com)
The map position of cDNA clones relative to the predicted location of quantitative trait loci will provide new opportunities to unravel the genetic control of polygenic traits in forest tree species. (le.ac.uk)
Development of comparative physical gene maps for cattle, sheep, goat and deer by fluorescence in situ hybridisation (FISH) using ovine bacterial artificial chromosome (BAC) clones. (edu.au)
1993) Isolation and FISH mapping of 80 cosmid clones on the short arm of human chromosome 3. (u-tokyo.ac.jp)
Hybridization of total genomic DNA to the library and analysis of individual clones indicated that ~26% of the clones contain single-copy DNA. (johnshopkins.edu)
In addition, a 550-kbp contig composed of 6 BAC clones was constructed that encompassed two adjacent RFLP markers on chromosome 2. (johnshopkins.edu)
All chromosome imbalances were size mapped using BAC and PAC clones based on clone mapping databases ( www.ensembl.org , July 2003), except for case 9, which was size mapped using a chromosome 22 specific micro-array. (bmj.com)

Using the 270 K Nimblegen comparative genomic hybridization (CGH) array on a set of cv. (usda.gov)
FISH and CGH (Comparative Genomic Hybridization) are molecular cytogenetic procedures commonly employed for genetic analysis. (ablogwithadifference.com)
Standard chromosome analysis by G-banding has a limited resolution, but molecular cytogenetic techniques, such as multi-subtelomeric FISH, microdeletion FISH, multicolour FISH and comparative genomic hybridisation (CGH), have played an important role for the diagnosis of MR during the past decade. (bmj.com)

Physical map is a technique used in molecular biology to find the order and physical distance between DNA base pairs by DNA markers. (wikipedia.org)
Genetic mapping, another approach of gene mapping, can provide markers needed for the physical mapping. (wikipedia.org)
Physical mapping uses DNA fragments and DNA markers to assemble larger DNA pieces. (wikipedia.org)
Markers from fragments of human chromosome in radiation hybrids give cross-reactivity patterns, which are further analyzed to generate a radiation hybrid map by ordering the markers and breakpoints. (wikipedia.org)
This provides evidence on whether the markers are located on the same human chromosome fragment, and hence the order of gene sequence. (wikipedia.org)
In this study a method of accurately placing thousands of markers onto chromosome intervals was evaluated. (usda.gov)
The chicken genetic map is about 4000 cM, with 2000+ molecular markers mapped using three major reference families. (kent.ac.uk)
We've assigned over 6000 BACs to over 600 markers across almost all chicken chromosomes and linkage groups. (kent.ac.uk)
Physical markers are, in particular. (centrocardiobogota.com)
This backcross analysis has defined the map position of my on mouse Chr 3 relative to DNA markers. (ucl.ac.uk)

Chromosome-mediated gene transfer is a process that coprecipitates human chromosome fragments with calcium phosphate onto the cell line, leading to a stable transformation of recipient chromosomes retaining human chromosomes ranging in size from 1 to 50 mega base pairs. (wikipedia.org)

Copies of colony hybridization membranes are available upon request. (johnshopkins.edu)

In the absence of a finished quality genome sequence, insights into wheat genome structure and function have been accumulating through survey sequencing of individual chromosomes or chromosome-specific Bacterial Artificial Chromosome (BAC) libraries. (biomedcentral.com)
Both bacterial artificial chromosome (BAC) and small insert sequencing libraries have been constructed from DNA of a single female inbred Red Jungle Fowl, the nearest wild relative of domestic chickens. (kent.ac.uk)
To facilitate M. grisea genome analysis, physical mapping, and positional cloning, we have constructed a bacterial artificial chromosome (BAC) library from the rice infecting strain 70-15. (johnshopkins.edu)
Transfers of a single gene, a complete metabolic pathway 10 , whole chromosomes 11 or even cases of the integration of almost complete genomes from bacterial endosymbionts into their eukaryotic hosts 12 have been described. (nature.com)

The establishment of synteny maps will provide a powerful tool to investigate the quantitative conservation of map location of trait loci across species and environments. (le.ac.uk)
Brassica napus (AACC) is an amphidiploid species that was formed by hybridization of two Brassica species that diverged approximately 4 million years ago, Brassica rapa (AA) and Brassica oleracea (CC) [ 10 , 11 ]. (biomedcentral.com)
In this sense, obtaining a high-density genome mapping of the species could offer clues to the aquaculture improvement in those aspects not resolved so far. (metasystems-international.com)
This kind of result widens the knowledge of this species' chromosome dynamics and evolution. (metasystems-international.com)
Cytogenetically, the species of the genus Melipona show variation in the amount and distribution of heterochromatin along their chromosomes and can be separated into 2 groups: the first with low content of heterochromatin and the second with high content of heterochromatin. (karger.com)
In general, Melipona species have 2n = 18 chromosomes, and the species of each subgenus share the same characteristics in relation to heterochromatin regions, DAPI/CMA 3 fluorophores, and the number and distribution of 18S rDNA sites. (karger.com)

In other cases, individuals have a shorter or longer duplicated segment within the q21.1 region of chromosome 1. (medlineplus.gov)
The core phenotype maps within the terminal 1.9 Mb region of chromosome 4p. (medscape.com)

We were building YAC [yeast artificial chromosome] libraries, cosmid libraries. (nih.gov)
High-resolution physical mapping by combined Alu-hybridization/PCR screening: construction of a yeast artificial chromosome map covering 31 centimorgans in 3p21-p14. (u-tokyo.ac.jp)

If you look at the karyotype, the chromosome map, you will see they are all different sizes. (nih.gov)
Routine karyotype analysis is not sensitive enough to detect subtle chromosome rearrangements (less than 5 Mb). (bmj.com)
A Comprehensive Integrated Genetic Map of the Complete Karyotype of Solea senegalensis (Kaup 1858). (metasystems-international.com)

Her research interests are in livestock molecular genetics and include the genome organization of Bovidae, genome mapping in Bovidae and genetics of lipid metabolism in cattle. (edu.au)
One such report appeared on January 26, 2001 on the, Genetics, Inc. regulating chromosome pairing in polyploid plants. (centrocardiobogota.com)

Our method of genotyping deletions on chromosome 7B relied on a model-based clustering algorithm (Mclust) to accurately predict the presence or absence of a given genomic sequence in a deletion line. (usda.gov)
Alterations in chromosome structure - deficiencies/deletions, duplications, inversions and translocations. (tsukuba.ac.jp)

5 megabase contig on mouse chromosome 1. (u-tokyo.ac.jp)

1993) Clinical and genetic studies of renal cell carcinomas in a family with a constitutional chromosome 3;8 translocation. (u-tokyo.ac.jp)

BAC fingerprint analyses and alignments of BACs to the pre-existing linkage map have generated a BAC contig physical map of the genome. (kent.ac.uk)

DNA from this somatic cell hybrid gave a positive result in Southern blot hybridization analysis with a probe for 21q22.1q22.2, D21S17. (coriell.org)

Construction of comprehensive, informative second generation linkage maps for three Conifer genomes. (le.ac.uk)
Initial studies with isozymes (1) indicate that pines exhibit conservation of gene order and this hypothesis will be examined further by the use of transcript based linkage maps. (le.ac.uk)
However, the utility of such maps is limited and there is a recognised need to produce second generation (STS based) linkage maps represented by living pedigrees. (le.ac.uk)
Subsequently, a gene for LD was mapped, by linkage studies, to a 16-cM region at 16q24.3. (nih.gov)

The method suggests the order of desired regions on the DNA sequence by analyzing the partial overlaps and gaps between yeast artificial chromosomes (YACs). (wikipedia.org)
A more detailed analysis of the critical region around my will allow the isolation of Yeast Artificial Chromosomes (YACs) which span the region and facilitate the subsequent physical mapping and gene isolation. (ucl.ac.uk)

It is one of the gene mapping techniques which can determine the sequence of DNA base pairs with high accuracy. (wikipedia.org)

Twenty years ago genetic studies were based not on disease but on chromosomes, because it was so hard to study a whole genome. (nih.gov)
19- 22 Yet none of these techniques is able to offer a high resolution screening of the whole genome for chromosome imbalances. (bmj.com)

was used to create chromosome-speciﬁc DNA libraries. (centrocardiobogota.com)
obtaining chromosome-speciﬁc DNA libraries [43]. (centrocardiobogota.com)

Here, we provide a chromosome-specific view into the organization and evolution of the D genome of bread wheat, in comparison to one of its ancestors, revealing recent genome rearrangements. (biomedcentral.com)
RUNX1 itself is the target of several chromosome rearrangements seen in leukemia. (nih.gov)
FISH offers high-resolution mapping of particular genetic regions while simultaneously revealing any structural changes or gene rearrangements that might exist within an organism's genomes. (ablogwithadifference.com)
Routine chromosome analysis (450-500 bands) had failed to detect the rearrangements in all cases. (bmj.com)

Comparison of genetic and synteny-based physical maps indicated that ∼50% of all genetic recombination is confined to 14% of the physical length of the chromosome arm in the distal region. (inrae.fr)
Wolf-Hirschhorn syndrome results from the deletion of the distal short arm of chromosome 4. (medscape.com)

A major mutation common in leukemia is called chromosome 16 inversion. (nih.gov)
In plants, the most dramatic form of mutation is polyploidization, the duplication of the chromosome set. (lu.se)

Humans normally have 46 chromosomes in each cell, divided into 23 pairs. (medlineplus.gov)

Despite the large chromosome fragments involved in the unbalanced translocation in this case, repeated standard G-band analysis could not reveal this rearrangement due to the similarity in the banding pattern of the chromosome fragments involved. (bmj.com)
Other changes in chromosome 4 can involve a ring structure or translocation. (medscape.com)

The high-quality physical map constructed in this study paves the way for the assembly of a reference sequence, from which breeding efforts will greatly benefit. (biomedcentral.com)
The goal of physical mapping, as a common mechanism under genomic analysis, is to obtain a complete genome sequence in order to deduce any association between the target DNA sequence and phenotypic traits. (wikipedia.org)
Irradiation fusion gene transfer produces radiation hybrids which contain the human sequence of interest and a random set of other human chromosome fragments. (wikipedia.org)
As part of the international effort to sequence the hexaploid bread wheat genome by the international wheat genome sequencing consortium (IWGSC) we are focused on assembling a reference sequence for chromosome 7B. (usda.gov)
The successful completion of the reference chromosome sequence is highly dependent on the integration of genetic and physical maps. (usda.gov)

If artificially stretched chromatin is used instead, mapping resolutions could be over 700 kilobases. (wikipedia.org)
An example of high resolution FISH mapping using stretched chromatin is extended chromatin fiber (ECF) FISH. (wikipedia.org)
General overview of chromosomes behavior during cell cycles - mitosis and meiosis, chromatin strcuture and molecular organization of the chromosomes. (tsukuba.ac.jp)

To aid the integration of these two types of maps, we have constructed a high-density deletion bin map of chromosome 7B. (usda.gov)
1p36 deletion syndrome is caused by a deletion of genetic material from a specific region in the short (p) arm of chromosome 1. (medlineplus.gov)
A large deletion several megabases (Mb) in length, easily detected using conventional chromosome analysis, is usually associated with severe phenotypic expression, including multiple malformations. (medscape.com)
Factors involved in prediction of prognosis include the extent of the deletion, the occurrence of complex chromosome anomalies, and the severity of seizures. (medscape.com)

In this study, we constructed the first comprehensive physical map of wheat chromosome arm 5DS, thereby exploring its gene space organization and evolution. (biomedcentral.com)
Bread wheat ( Triticum aestivum L.) originated from a spontaneous hybridization between the cultivated tetraploid wheat Triticum turgidum L. (2n = 4x = 28, AABB genome) and the wild diploid grass Aegilops tauschii Coss. (biomedcentral.com)
Bread wheat (Triticum aestivum L.) has a large, complex and highly repetitive genome which is challenging to assemble into high quality pseudo-chromosomes. (usda.gov)
A Physical Map of the Short Arm of Wheat Chromosome 1A. (inrae.fr)
To aid map-based cloning and future genome sequencing projects, we constructed a BAC-based physical map of the short arm of wheat chromosome 1A (1AS). (inrae.fr)
Construction of chromosome specific BAC library plays an important role for simplifying sequencing, physical mapping and gene cloning of plant with complexity genome such as common wheat, identification of sorted chromosome is a vital step of library construction. (chinacrops.org)
Identification of Wheat Chromosomes Sorted by Flow Cytometry[J]. Acta Agron Sin, 2008, 34(01): 89-94. (chinacrops.org)

One more to note is that if metaphase chromosome is used in FISH mapping, the resolution resulted will be very poor, which is to be classified to low-resolution mapping rather than a high-resolution mapping. (wikipedia.org)
Papillary renal cell carcinoma: quantitation of chromosomes 7 and 17 by FISH, analysis of chromosome 3p for LOH, and DNA ploidy. (u-tokyo.ac.jp)

As a result, statistical analysis is necessary to generate the accurate map order of interphase chromosomes. (wikipedia.org)
Practicals: chromosome analysis in plant and animals. (tsukuba.ac.jp)

Chastain-Moore AM, Roberts T, Trott DA, Newbold RF, Ornelles DA , An activity associated with human chromosome 21 permits nuclear colocalization of the adenovirus E1B-55K and E4orf6 proteins and promotes viral late gene expression. (coriell.org)

Contain a diploid set of chromosomes and make up the majority of human cells. (proprofs.com)

Segregating populations will be characterised with both single locus (microsatellites) and multi-locus (AFLPs) marker systems providing framework maps for the eventual location of cDNAs generated from single pass sequencing. (le.ac.uk)
This is the first assignment of a U13 marker to a bovine chromosome. (tamu.edu)

Two copies of chromosome 1, one copy inherited from each parent, form one of the pairs. (medlineplus.gov)
Chromosome 1 is the largest human chromosome, spanning about 249 million DNA building blocks (base pairs) and representing approximately 8 percent of the total DNA in cells. (medlineplus.gov)

The correct application of selection approaches for superior genotypes is directly dependent of the chromosome behavior during the process of cell division - mitosis and meiosis. (tsukuba.ac.jp)

Most phenotypic manifestations in this syndrome reflect a contiguous gene syndrome, leading to a phenotypic map of chromosome arm 4p. (medscape.com)

it allows mapping and visualization, making this technique ideal for the diagnosis of genetic anomalies such as abnormalities or changes to chromosomes as well as oncology screening or prenatal detection. (ablogwithadifference.com)

As a result, important data about its genome organization and evolution were obtained, such as the lesser gene density of the large metacentric pair compared with the other metacentric chromosomes, which supports the theory of a sex proto-chromosome pair. (metasystems-international.com)

Anatomy51

Organisms3

Diseases17

Chemicals and Drugs22

Analytical, Diagnostic and Therapeutic Techniques and Equipment34

Psychiatry and Psychology1

Phenomena and Processes130

Disciplines and Occupations3

Anthropology, Education, Sociology and Social Phenomena3

Information Science4

Health Care2

Situ hybridization20

Genes18

Metaphase chromosomes2

Fluorescent1

Sequences10

Probes6

Contigs7

Chromosomal6

Polymerase chain re2

Clones7

Comparative3

Markers10

Human chromosomes1

Colony hybridization1

Bacterial4

Species6

Region of chromosome2

Yeast artificial2

Karyotype3

Genetics2

Deletions2

Mouse chromosome1

Constitutional chromosome1

Contig1

Somatic cell hybrid1

Linkage4

YACs2

GENE MAPPING1

Whole genome2

Libraries2

Rearrangements4

Distal2

Mutation2

Humans1

Translocation2

Sequence5

Chromatin3

Deletion4

Wheat7

FISH2

Analysis2

Proteins1

Diploid1

Marker2

Pairs2

Meiosis1

Phenotypic1

Anomalies1

Genome organization1