• Fluorescence microscopy can be used to find out where the fluorescent probe is bound to the chromosomes. (wikipedia.org)
  • Advances in fluorescence microscopy, fluorescent dyes, biotechnology, bioinformatics, and research on the human genome project in the late 90's accelerated the development of methods to synthesize and design NA probes for FISH. (springer.com)
  • Fluorescence in situ hybridization (FISH) is a molecular cytogenetic technique that uses fluorescent probes that bind to only particular parts of a nucleic acid sequence with a high degree of sequence complementarity. (wikipedia.org)
  • The continuous flow of probes and shaping liquid on these selected cells resulted in a 120-fold reduction of the hybridization time compared with the standard protocol (3 min vs. 6 h) and efficient rinsing, thereby shortening the total FISH assay time for centromeric probes. (springer.com)
  • A key milestone in ISH-based techniques was fluorescence in situ hybridization (FISH) (Bauman et al. (springer.com)
  • Therefore, we analyzed the potential of fluorescence in situ hybridization (FISH) for diagnosing WD, using a Tropheryma (T.) whipplei -specific probe. (frontiersin.org)
  • The characterisation of the lymphoma cell line U937, using comparative genomic hybridisation and multi-plex FISH. (wikidata.org)
  • It is critical for the hybridization process to have all optimal conditions to have a successful in situ result, including temperature, pH, salt concentration, and time of the hybridization reaction. (wikipedia.org)
  • The process is done in 3 main procedures: tissue preparation (pre-hybridization), hybridization, and washing (post-hybridization). (wikipedia.org)
  • The hybridization signals for each probe when a nucleic abnormality is detected. (wikipedia.org)
  • After checking all the necessary conditions, hybridization steps can be started by first adding a target-specific probe, composed of 20 oligonucleotide pairs, hybridizes to the target RNA(s). (wikipedia.org)
  • The probe must be large enough to hybridize specifically with its target but not so large as to impede the hybridization process. (iiab.me)
  • At the end of the assay the tissue samples are visualized under a fluorescence microscope. (iiab.me)
  • It is critical for the hybridization process to have all optimal conditions to have a successful in situ result, including temperature, pH, salt concentration, and time of the hybridization reaction. (wikipedia.org)
  • After fixation, samples are permeabilized to allow the penetration of hybridization reagents. (wikipedia.org)