• Beginning in 1976, Nurse identified the gene cdc2 in fission yeast (Schizosaccharomyces pombe). (wikipedia.org)
  • The genome sequence of Schizosaccharomyces pombe. (genscript.com)
  • Schizosaccharomyces pombe YEATS family histone acetyltransferase subunit Yaf9 (yaf9), mRNA. (genscript.com)
  • In the February 21 Nature, an international consortium of laboratories, led by the British Nobel laureate Paul Nurse, reports the complete sequence of the fission yeast Schizosaccharomyces pombe (Nature 2002, 415:871-880).The depth of sequence coverage was about eight-fold. (the-scientist.com)
  • Professor Forsburg studies how chromosome duplication and maintenance contributes to overall genome stability using a simple model genetic system, the fission yeast Schizosaccharomyces pombe . (usc.edu)
  • It shares homology with the mating-type protein, Mc, from the fission yeast Schizosaccharomyces pombe and a conserved DNA-binding motif present in the nuclear high-mobility-group proteins HMG1 and HMG2. (nih.gov)
  • We have characterized a Schizosaccharomyces pombe homologue of the ORC subunit, Orc4p. (embl.de)
  • Homologues could be found, however, in predicted protein sequences from Schizosaccharomyces pombe. (pasteur.fr)
  • We have cloned the RAD6 homolog from Drosophila melanogaster and find that its encoded protein displays a very high degree of identity in amino acid sequence with the homologous RAD6 proteins from the two divergent yeasts, S. cerevisiae and Schizosaccharomyces pombe, and from human. (eur.nl)
  • Human Rad 9 ( hRad9 ) was originally identified as a structural homologue of yeast schizosaccharomyces pombe rad 9 , which can partially rescue the sensitivity of rad 9 null yeast to hydroxyurea, radiation damage and the associated checkpoint defects [ 1 ]. (biomedcentral.com)
  • For our work, we mainly use a simple eukaryotic model organism, the fission yeast Schizosaccharomyces pombe . (unige.ch)
  • The goal of this thesis was to characterize how cells regulate signaling of cytokinesis, both positively and negatively, during cell division using the fission yeast Schizosaccharomyces pombe as a model organism. (umassmed.edu)
  • In the fission yeast Schizosaccharomyces pombe, deletion of pot1+ results in rapid telomere loss, and the resulting survivors have circular chromosomes. (bvsalud.org)
  • Here we describe the isolation and characterization of pfh1 + , a Schizosaccharomyces pombe gene that encodes a Pif1-like protein. (princeton.edu)
  • Here we describe the isolation and characterization of pfh1+, a Schizosaccharomyces pombe gene that encodes a Pif1-like protein. (princeton.edu)
  • The swiss-prot protein sequence for schizosaccharomyces pombe clr4 is o60016. (shopambitionhustle.com)
  • Benvenisty, N. (1998) Characterization of a branched-chain amino-acid aminotransferase from Schizosaccharomyces pombe Yeast 14 189-94. (unipr.it)
  • His PhD dissertation entitled "Genetic approaches to the study of Rheb GTPase" utilized the model organisms, Saccharomyces cerevisiae and Schizosaccharomyces pombe , to elucidate Rheb's function in arginine uptake and nutrient sensing. (usc.edu)
  • He further utilized the model organism, Schizosaccharomyces pombe , to investigate the role of the SPN/FACT complex in eukaryotic DNA replication. (usc.edu)
  • We have developed a functional "no-hybrids" screen in the fission yeast Schizosaccharomyces pombe based on the transcription transactivator activity of human p53. (ox.ac.uk)
  • To understand better the contribution of hypermutagenesis to tumour development, we have modelled the most recurrent POLE-EDM (POLE-P286R) in Schizosaccharomyces pombe. (ox.ac.uk)
  • In a new study in the Journal of Agricultural and Food Chemistry , Wang and coworkers were able to toughen up budding yeast against caffeine by adding bfr1 , a gene from Schizosaccharomyces pombe that encodes the ABC transporter that shunts caffeine out of the cell. (yeastgenome.org)
  • Telomeric silencing has been demonstrated in organisms ranging from yeasts to humans (reviewed in [ 9 ]) but it has been best characterized in S. cerevisiae , Schizosaccharomyces pombe and Drosophila melanogaster , organisms that have no, or very little, DNA methylation. (biomedcentral.com)
  • The fission yeast S. pombe lacks Sir3p and Sir4p but employs methylation of histone H3 lysine 9 (K9) plus a Sir2p homologue (Sir2) for silencing [ 15 ]. (biomedcentral.com)
  • However, a Borealin homologue has never been characterized in the fission yeast, Schizosaccharomyces pombe. (uky.edu)
  • Nbl1 (novel Borealin-like 1) physically associates with known CPC components, affects the kinase activity and stability of the S. pombe Aurora B homologue, Ark1, colocalizes with known CPC subunits during mitosis, and shows sequence similarity to human Borealin. (uky.edu)
  • Thus, this study characterizes a fission yeast Borealin homologue and reveals a previously unrecognized connection between the CPC and the process of cytokinesis in S. pombe. (uky.edu)
  • A model system where the targeting of the chromosomes to the spindle can be studied is meiosis I in the fission yeast Schizosaccharomyces pombe ( S. pombe ). (nature.com)
  • At the onset of meiosis I in the fission yeast S. pombe , the KCs are far apart from SPBs and telomeres, and SPBs are clustered together 2 . (nature.com)
  • In the fission yeast Schizosaccharomyces pombe genes embedded in histone H3 lysine 9 methylation (H3K9me) heterochromatin, deposited by the sole H3K9- methyltransferase Clr4, are transcriptionally silenced. (ed.ac.uk)
  • Functional analysis of lncRNAs in fungi has mainly been carried out in the yeast species, Saccharomyces cerevisiae and Schizosaccharomyces pombe . (biomedcentral.com)
  • Here we identify causes underlying hybrid infertility of two recently diverged fission yeast species Schizosaccharomyces pombe and S. kambucha , which mate to form viable hybrid diploids that efficiently complete meiosis, but generate few viable gametes. (elifesciences.org)
  • have now looked at two species of fission yeast- S. pombe and S. kambucha -that share 99.5% of their DNA sequence. (elifesciences.org)
  • The C-terminal domain shows strong sequence similarity to human, frog, and yeast Orc4 proteins, including conserved ATP-binding motifs. (embl.de)
  • Mitochondrial ribosomal proteins were studied best in yeast, where the small subunit was shown to contain about 35 proteins. (pasteur.fr)
  • Using a recently described affinity purification technique and tagged versions of yeast Ykl155c and Mrp1, we isolated this mitochondrial ribosomal subunit and identified a total of 20 proteins, of which 12 are new. (pasteur.fr)
  • For a subset of the newly described ribosomal proteins, we showed that they are localized in mitochondria and are required for the respiratory competency of the yeast cells. (pasteur.fr)
  • In more distant species, putative homologues were detected for Ykl155c, which shares conserved motifs with uncharacterized proteins of higher eukaryotes including humans. (pasteur.fr)
  • The yeast cell wall is a complex structure comprising proteins, lipids, and at least two types of polysaccharides: mannan and glucan. (microbiologynote.com)
  • The human MutS and MutL proteins form heterodimeric complexes that mediate the initial steps of MMR, including the recognition of mismatched base(s) arising from errors in replication, and signaling downstream proteins to facilitate mismatch removal. (elsevierpure.com)
  • S. cerevisiae is also a critical model organism in cell biology research, aiding in the understanding of eukaryotic cell and human biology. (microbiologynote.com)
  • The term "yeast" is frequently synonymous with S. cerevisiae, though this does not reflect the full phylogenetic diversity of yeast species. (microbiologynote.com)
  • Commercial exploitation of yeast began in the late 18th century, with strains like S. cerevisiae and S. pastorianus being identified for brewing. (microbiologynote.com)
  • The S. cerevisiae checkpoint protein Rad17, the orthologue of human Rad1, forms a homocomplex in response to treatment with DNA damaging agents, and the complex is required for yeast survival after exposure to genotoxic agents [ 12 ]. (biomedcentral.com)
  • When Wang and coworkers added the gene to S. cerevisiae , this newly engineered yeast could now better tolerate caffeine. (yeastgenome.org)
  • Fission yeast is a single-celled organism with chromosomes very similar to those in humans, and uses the same genes to maintain those chromosomes. (usc.edu)
  • This organism only encodes 5000 genes but 70% are conserved in humans. (unige.ch)
  • Cloning cell cycle regulatory genes by transcomplementation in yeast. (ox.ac.uk)
  • The majority of these cancer cases are caused by mutations in the human homologs of the E. Coli mutS and mutL genes. (elsevierpure.com)
  • Our proposed studies will not only identify novel interactions and/or functions of the MMR genes, but also have the potential to identify highly effective chemotherapeutic strategies for a prevalent human cancer syndrome. (elsevierpure.com)
  • We are studying yeast and mammalian cell lines with mutations in key MMR genes to identify key conserved genetic networks for MMR. (elsevierpure.com)
  • Elevated oxidative stress triggers transcriptional reprogramming via the fission yeast oxidative stress response pathway, leading to upregulation of efflux pumps and antioxidant genes, resulting in caffeine and multidrug cross-resistance, explaining the phenotype of epimutants. (ed.ac.uk)
  • RePub, Erasmus University Repository: DHR6, a Drosophila homolog of the yeast DNA repair gene RAD6. (eur.nl)
  • DHR6, a Drosophila homolog of the yeast DNA repair gene RAD6. (eur.nl)
  • Of course unlike even the most grizzled 30 year coffee drinker with ideal genetics, the yeast almost certainly gets no joy from its morning Joe. (yeastgenome.org)
  • Accurate duplication and segregation of our chromosomes is critical for human development and well-being. (usc.edu)
  • Fission yeast is a simple, single-celled organism with chromosomes very similar to those in humans, and has proven to be an important model for cell division. (usc.edu)
  • Circular chromosomes are found in humans and are often unstable during mitosis, resulting in genome instability. (bvsalud.org)
  • Fission yeast is a good model for studying the formation and stability of circular chromosomes as deletion of pot1 (encoding a telomere protection protein) results in rapid telomere degradation and chromosome fusion. (bvsalud.org)
  • Loss of pot1 leads to viable strains in which all three fission yeast chromosomes become circular. (bvsalud.org)
  • Although hybrids of these two species inherit three chromosomes from each parent, the majority of spores (the yeast equivalent of sperm) that these hybrids produce fail to develop into new yeast cells. (elifesciences.org)
  • Some fungi have been used as model systems to investigate the role of PPIases within the cell, however how representative these repertoires are of other fungi or humans has not been fully investigated. (biomedcentral.com)
  • Twelve of the 17 human cyclophilins and both human parvulins, but only one of the 13 human FKBPs, identified orthologues within these fungi. (biomedcentral.com)
  • Yeast, a unicellular eukaryotic organism , belongs to the kingdom Fungi. (microbiologynote.com)
  • The first microscopic observation of yeast was by Anton van Leeuwenhoek in 1680, although their classification as fungi was established later by Theodor Schwann in 1837. (microbiologynote.com)
  • Other species have been used in biotechnological processes: treatment of wood pulp wastes, production of food and fodder yeast (single cell protein), production of SCP from whey and petroleum, production of gums (mannans), polyhydroxy alcohols (glycerol) and glycolipids. (thecanadianencyclopedia.ca)
  • Human DNA topoisomerase II-binding protein 1 (hTopBP1) plays an important role in DNA replication and the DNA damage checkpoint pathway. (biomedcentral.com)
  • Human DNA topoisomerase II-binding protein 1 (TopBP1) and its orthologs play important roles in DNA replication and checkpoint control [ 1 ]. (biomedcentral.com)
  • A protein discovered in the late 1970s has been shown to defend human cells from becoming cancerous. (shopambitionhustle.com)
  • Industrially, yeast plays a pivotal role in baking and brewing, and also serves as a source for protein, biofuel production, and the commercial synthesis of various enzymes and organic compounds. (microbiologynote.com)
  • Glutaminase liver isoform, mitochondrial is a protein in humans that is encoded by GLS2 gene. (cusabio.com)
  • For instance, Human Protein Atlas localization and expression information is only available for human gene entries. (gene-info.org)
  • You can choose which resources to display for localization, including the Compartments subcellular localization database, the Human Protein Atlas and/or UniProt. (gene-info.org)
  • The Rad1 protein, evolutionarily conserved from yeast to humans, exists in cells as monomer as well as a component in the 9-1-1 protein complex. (biomedcentral.com)
  • Although ORC appears to play an essential role in the initiation of DNA replication in the cells of all eukaryotes, its interactions with DNA have not been defined in species other than budding yeast. (embl.de)
  • One yeast species is pathogenic for cotton. (thecanadianencyclopedia.ca)
  • Distinguished as eukaryotic microorganisms, yeasts encompass over 1,500 recognized species, forming about 1% of all described fungal species. (microbiologynote.com)
  • Yeast sizes vary, typically measuring 3-4 µm in diameter, although some species can expand up to 40 µm. (microbiologynote.com)
  • Asexual reproduction in yeasts predominantly occurs through mitosis , with many species employing budding. (microbiologynote.com)
  • You can query by locus or ORF for other species by selecting the appropriate option from the settings dropdown, however it will only autodetect these query types in yeast, flies and worms as these species adhere to specific naming formats that do not necessarily hold in other species. (gene-info.org)
  • Since these two yeast species are so closely related, the findings of Zanders et al. (elifesciences.org)
  • The fission yeast homologue of Orc4p binds to replication origin DNA via multiple AT-hooks. (embl.de)
  • We have recently shown that MTs explore the space by pivoting around the SPB, which accelerates KC capture in mitosis in fission yeast 9 . (nature.com)
  • Although not as widely distributed as Bacteria , yeasts occur in terrestrial and aquatic environments worldwide in association with plants and animals. (thecanadianencyclopedia.ca)
  • Distinctly larger than most bacteria, yeast is non-motile, lacking flagella or other locomotive structures. (microbiologynote.com)
  • A search of a 35-kilobase region of the human Y chromosome necessary for male sex determination has resulted in the identification of a new gene. (nih.gov)
  • In fact, like a lot of other microorganisms, caffeine actually kills this yeast. (yeastgenome.org)
  • TTG transitions are not elevated, likely due to the absence of cytosine methylation in fission yeast. (ox.ac.uk)
  • Hutson, S.M. (1997) Cloning of the rat and human mitochondrial branched chain aminotransferases (BCATm) Biochim Biophys Acta 1339 9-13. (unipr.it)
  • Considering the conservation of cell cycle regulators in the eukaryotic kingdom, it is likely that similar mechanisms to control cytokinesis exist in humans. (umassmed.edu)
  • A yeast cell is a single-celled, eukaryotic microorganism belonging to the fungus kingdom, characterized by its ability to ferment sugars into alcohol and carbon dioxide. (microbiologynote.com)
  • In the present study, we combine experiments and theory to explore the mechanisms of kinetochore capture at the onset of meiosis I in fission yeast. (nature.com)
  • Dr Tabancay's current research interests lie in the fields of pharmacy education, signal transduction pathways leading to cancer, DNA replication, and the use of model organisms to study human diseases. (usc.edu)
  • Yeast cells vary in size and shape, reproduce mainly asexually through budding, and are found in diverse environments, particularly those rich in sugars. (microbiologynote.com)
  • LncRNA RZE1 regulates zinc finger transcription factor ZNF2 and affects the yeast-to-hypha transition in the human pathogenic fungus Cryptococcus neoformans [ 22 ]. (biomedcentral.com)
  • Other yeasts, such as Candida albicans, are opportunistic pathogens in humans. (microbiologynote.com)
  • Fungal pathogens are a growing threat to human health, food security and ecosystem biodiversity. (ed.ac.uk)
  • In 1987, Nurse identified the homologous gene in human, Cdk1, which codes for a cyclin dependent kinase. (wikipedia.org)
  • You can imagine adding the caffeine pathway from coffee to yeast and having the yeast merrily exporting caffeine to the culture medium where it can be harvested. (yeastgenome.org)
  • Although the yeast don't get any of the pleasurable effects of caffeine, at least they can now happily grow in cultures that have more caffeine than a strong cup of coffee. (yeastgenome.org)
  • And who knows, maybe they can have the yeast make caffeine and alcohol at the same time creating the equivalent of a vodka and Red Bull in a single step! (yeastgenome.org)
  • For example, whereas wild type yeast barely grew with 8 mg/ml caffeine, the engineered yeast did OK. (yeastgenome.org)
  • While not as robust as bfr1-B , this mutant allowed yeast expressing it to tolerate caffeine concentrations up to 15 mg/ml, conditions under which cells with wild type bfr1 failed to grow. (yeastgenome.org)
  • In the end we have a bullet-proof yeast when it comes to caffeine that should prove useful for anyone who wants yeast to synthesize caffeine for them. (yeastgenome.org)
  • Here we probe these fundamental questions using peroxisomal compartmentalization of the last steps of lysine and histidine biosynthesis in the fission yeast Schizosaccharomyces japonicus . (biorxiv.org)
  • We use the mating response of yeast cells, during which they polarize growth in response to pheromones, to probe the mechanisms of gradient sensing and cell pairing. (unige.ch)
  • Additionally, yeasts are harnessed in microbial fuel cells and ethanol production for biofuels. (microbiologynote.com)
  • Yeast cells demonstrate high polymorphism, meaning their shapes can vary considerably depending on the environment and the cell's age. (microbiologynote.com)
  • A new tumor model, combining human embryonic stem cells (ESC) and tumor cells, develops abundant human vessels. (nih.gov)
  • We hypothesize that ovarian cancer cells will induce human ovarian TVM expression. (nih.gov)
  • Finally, our preliminary data suggest that the ESC-ovarian tumor model has human tumor vascular cells. (nih.gov)
  • We hypothesize that the ESC ovarian tumor model, with human vascular cells, will provide an ideal microenvironment to support human stem cell growth. (nih.gov)
  • If successful, this will create a murine tumor model that nearly completely reproduces the human tumor microenvironment with human tumor stroma, vessels and tumor stem cells. (nih.gov)
  • Besides the existence of 9-1-1 heterotrimer in K562 and 293 human cells, a significant amount of hRad1 also exists in monomeric form, but monomeric hRad9 and hHus1 were not detectable in a study by Karnitz's group [ 10 ] and in our unpublished experiments in 293 human cells. (biomedcentral.com)
  • Working in fission yeast, Nurse identified the gene cdc2, which controls the transition from G1 to S, when the cell grows in preparation for the duplication of DNA, and G2 to M, when the cell divides. (wikipedia.org)
  • In 2003, he became president of Rockefeller University in New York City where he continued work on the cell cycle of fission yeast. (wikipedia.org)
  • Most yeasts belong to the subdivision Ascomycotina, which multiply by budding in various ways or by transverse fission, and may form ascospores in a naked ascus (saclike cell). (thecanadianencyclopedia.ca)
  • Our recent work established fission yeast as a simple model to investigate the flow of sterols through the cell and the role of the actin cytoskeleton in this flow. (unige.ch)
  • The structure of a yeast cell includes a distinct cell wall, granular cytoplasm, a prominent vacuole , and a nucleus. (microbiologynote.com)
  • The vacuole within a yeast cell, which can vary in size based on the cell's activity, plays a significant role in cell metabolism and storage. (microbiologynote.com)
  • We will utilize this information to predict (and test) synthetic sick or lethal interactions in defined MMR- deficient mouse and human cancer cell lines. (elsevierpure.com)
  • Vinyl chloride induced sister chromatid exchanges in human lymphocytes in vitro . (inchem.org)
  • The fission yeast Schizosaccharomyces japonicus provides an attractive stripped-down model for metabolic compartmentalization in peroxisomes. (biorxiv.org)
  • Development of anti-vascular immunotherapeutics, with few exceptions, has been hindered by the absence of a tumor model with human tumor vessels. (nih.gov)
  • With his postdoc Melanie Lee, Nurse also found the corresponding gene, CDK1, in humans. (wikipedia.org)
  • A "no-hybrids" screen for functional antagonizers of human p53 transactivator function: dominant negativity in fission yeast. (ox.ac.uk)
  • Expression of functional full-length human p53 is conditionally lethal to the screen reporter strains. (ox.ac.uk)
  • Maybe this genetic engineered yeast can make the equivalent of a Red Bull and vodka all in one step! (yeastgenome.org)
  • Microbiome research is a rapidly developing area of science and innovation, seeking to explore and exploit the complex communities of microbes associated with humans, animals, plants and other environments such as soils and oceans. (microbiologysociety.org)