• clones
  • IMPORTANCE The genomes of large DNA viruses, such as human cytomegalovirus (HCMV), are difficult to manipulate using current genetic tools, and at this time, it is not possible to obtain, molecular clones of CMV without extensive tissue culture. (asm.org)
  • Collectively, the BAC library and pooling system of the BAC clones from the Korean cattle, coupled with the chromosome-localized BAC clones, will provide us with novel tools for the excavation of desired clones for genome mapping and sequencing, and will also furnish us with additional information regarding breed differences in cattle. (koreascience.or.kr)
  • novo
  • HACs were first constructed de novo in 1997 by adding alpha-satellite DNA to telomeric and genomic DNA in human HT1080 cells. (wikipedia.org)
  • region
  • This region shows a significant divergence between human and chimpanzee, suggesting that its high mutation rates have contributed to the evolution of the human brain. (wikipedia.org)
  • models
  • HACs have been used to create transgenic animals for use as animal models of human disease and for production of therapeutic products. (wikipedia.org)
  • total
  • Chromosome 8 spans about 145 million base pairs (the building material of DNA) and represents between 4.5 and 5.0% of the total DNA in cells. (wikipedia.org)
  • cells
  • 21HAC was also able to be transferred into cells from a variety of species (mice, chickens, humans). (wikipedia.org)
  • Their study revealed the chromosome rearrangements and CNV of MCF7 human cancer cells at a 150kb resolution, which is much more accurate compared to both CGH and spectral karyotyping at that time. (wikipedia.org)