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CentromereCentromere Protein BChromosomal Proteins, Non-HistoneDNA, SatelliteChromosomes, Artificial, HumanAutoantigensMitosis ModulatorsKinetochoresHeterochromatinMitosisChromatinChromosome SegregationChromosomesDNA-Binding ProteinsChromosomes, FungalBase SequenceMolecular Sequence DataMeiosisHeLa CellsIn Situ Hybridization, FluorescenceSchizosaccharomycesMetaphaseChromatidsHistonesChromosomes, PlantAnaphaseAurora Kinase BSpindle ApparatusDNA, FungalSchizosaccharomyces pombe ProteinsAurora KinasesSaccharomyces cerevisiaeCell Cycle ProteinsMuntjacsChromosomes, HumanChromosome PairingSynaptonemal ComplexChromosome MappingProphaseSaccharomyces cerevisiae ProteinsNondisjunction, GeneticIsochromosomesInterphaseNucleosomesTelomereMeiotic Prophase IFungal ProteinsTelophaseNuclear ProteinsRepetitive Sequences, Nucleic AcidCREST SyndromeMicrotubulesEuchromatinRecombination, GeneticRetroelementsZea maysChromosome PositioningMutationSaccharomycetalesCrossing Over, Genetic
ProteinProteinsKinetochoreAnti-centromereChromosome segregationChromatinAutoantibodiesFunctionalHistoneSequenceSequencing the human genomeChromosomalSequencesAntibodyCentromericAssemblyConditionalMammalianEpithelial cellsCellsGenomicRepeatsGenesMitoticAneuploidyUrineIdentityMutantsProbeCenpbVariationCellularSynonymeMechanismsMutationsMolecular GeneticsSpeciesSyndromeVitroSimilarityNCBIMappingProductsFluorescentDisease
Protein18
  • Centromere protein B also known as major centromere autoantigen B is an autoantigen protein of the cell nucleus. (wikipedia.org)
  • In humans, centromere protein B is encoded by the CENPB gene. (wikipedia.org)
  • Centromere protein B is a highly conserved protein that facilitates centromere formation. (wikipedia.org)
  • This protein is proposed to play an important role in the assembly of specific centromere structures in interphase nuclei and on mitotic chromosomes. (wikipedia.org)
  • Centromere protein B is a potential biomarker of small-cell lung cancer. (wikipedia.org)
  • Entrez Gene: centromere protein B". Sugimoto K, Yata H, Himeno M (July 1993). (wikipedia.org)
  • Centromere protein C 1 is a centromere autoantigen and a component of the inner kinetochore plate. (antibodies-online.com)
  • Description: A sandwich quantitative ELISA assay kit for detection of Human Centromere Protein F (CENPF) in samples from tissue homogenates, cell lysates or other biological fluids. (kits-elisa.com)
  • Description: A competitive ELISA for quantitative measurement of Human Centromere protein F(CENPF) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. (kits-elisa.com)
  • Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Centromere Protein F (CENPF) in Tissue homogenates, cell lysates and other biological fluids. (kits-elisa.com)
  • Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Centromere Protein F (CENPF) in samples from Tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species. (kits-elisa.com)
  • ACA recognises centromere â € complex' rather than individual protein, and this feature is common among patients with SS, SSc and PBC. (elsevierpure.com)
  • Most eukaryotic centromeres are defined epigenetically by the histone H3 variant, centromere protein (CENP)-A, yet how its self-propagation is achieved remains poorly understood. (researchgate.net)
  • For each protein, basic characteristics about its composition and length, its human relatives and relatedness to proteins in other species, and direct links to resources at NCBI are included. (cshlpress.com)
  • The entire text of the Guide is searchable, and tools are available for identifying human protein sequences using those from other species. (cshlpress.com)
  • Sandwalk: How many human protein-coding genes are essential for cell survival? (blogspot.com)
  • The human genome contains about 20,000 protein-coding genes and about 5,000 genes that specify functional RNAs. (blogspot.com)
  • Mn, serum Mn, urine Mn, Centromere-positive micronuclei in periphery blood lymphocytes, DNA protein-crosslinks in peripheral white blood cells, oxidative stress as urinary 8OHdG, and long-term oxidative damage measured as erythrocytic superoxide dismutase (SOD) activity (measure of systemic oxidative stress) and serum malondialdehyde (MDA) (product of lipid peroxidation and reflect tissue injuries). (who.int)
Proteins4
  • In the online version, these sections contain links to more information about proteins encoded by over 17,000 known or predicted human genes. (cshlpress.com)
  • Additional links to NCBI resources are provided for human noncoding RNAs and repeated DNA elements and for proteins of interest from other species. (cshlpress.com)
  • How similar are human proteins to those of mouse, yeast, or bacteria? (cshlpress.com)
  • Ignoring the edges, they isolated certain proteins found only at centromeres from two different types of human cells, male and female, and looked at the DNA stuck to those centromere proteins in an unbiased way. (fredhutch.org)
Kinetochore1
  • HZwint-1, a novel human kinetochore component that interacts with HZW10. (joplink.net)
Anti-centromere3
  • It is also considered a major centromere autoantigen recognized by sera from patients with anti-centromere antibodies. (wikipedia.org)
  • CENP-B specific anti-centromere autoantibodies heralding small-cell lung cancer. (wikipedia.org)
  • This study aims to clarify overall of autoantibody production at lesion site, including anti-centromere antibody (ACA)-positive SS. (elsevierpure.com)
Chromosome segregation2
Chromatin5
  • The functional identity of centromeres arises from a set of specific nucleoprotein particle subunits of the centromeric chromatin fibre. (nuigalway.ie)
  • CRISPR imaging reveals chromatin fluctuation at the centromere region related to cellular senescence. (bvsalud.org)
  • The human genome is spatially and temporally organized in the nucleus as chromatin , and the dynamic structure of chromatin is closely related to genome functions. (bvsalud.org)
  • Here, we report chromatin dynamics at the centromere region during senescence in cultured human cell lines using live imaging based on the clustered regularly interspaced short palindromic repeat /dCas9 system. (bvsalud.org)
  • Our findings suggest that the increased fluctuation of the chromatin structure facilitates centromere disorganisation during cellular senescence . (bvsalud.org)
Autoantibodies1
  • Testing of IgG autoantibodies to human cellular antigens was performed by the HEp-2 cell immunofluorescence assay using slides from INOVA Diagnostics, San Diego, CA (Cat # 508100) following the manufacturer's instructions and evaluated primarily using their NOVA View system. (cdc.gov)
Functional5
  • Genomic and functional variation of human centromeres. (duke.edu)
  • Here, we discuss the organization of human centromeres as well as genomic and functional variation in human centromere location, and current understanding of the genomic and epigenetic mechanisms that underlie centromere flexibility in humans. (duke.edu)
  • Functional epialleles at an endogenous human centromere. (duke.edu)
  • In addition to the functional and structural role within centromere they are also a suitable model for evolutionary stud-ies, because of being subject to concerted evolution. (srce.hr)
  • I didn't believe that this approach would identify the functional centromere. (fredhutch.org)
Histone1
  • These include CENP-A and histone H3 nucleosomes and a novel nucleosome-like complex of CENPs -T, -W, -S and -X. Fluorescence cross-correlation spectroscopy and Forster resonance energy transfer (FRET) revealed that human CENP-S and -X exist principally in complex in soluble form and retain proximity when assembled at centromeres. (nuigalway.ie)
Sequence8
  • The sequence of the Biotin-labeled centromere probe AAACTAGACAGAAGCATT is 5' labeled by the Biotin fluorescent Dye. (eurogentec.com)
  • Last month I explained how the difference in DNA sequence between chimps and humans corresponds to what we would predict from evolutionary theory. (blogspot.com)
  • The first complete, gapless sequence of a human genome. (genome.gov)
  • Video: How to Sequence a Human Genome in 7 'Easy' Steps! (genome.gov)
  • The Human Genome Project ended in 2003, but genomic researchers had not yet determined every last base (or letter) of the human genome sequence. (genome.gov)
  • Using a recent assembly of the human genome sequence, Stewart Scherer has compiled answers to a broad range of questions about the structure and function of the human genome. (cshlpress.com)
  • The techniques the team used weren't available when the human genome sequence was first published. (fredhutch.org)
  • The repetitive sequence at the centromere region, alpha- satellite DNA , was predominantly detected on chromosomes 1, 12, and 19. (bvsalud.org)
Sequencing the human genome1
  • James Watson describes sequencing the human genome using markers and BACs, and Craig Venter explains using cDNA libraries, ESTs, and shotgun sequencing. (cshl.edu)
Chromosomal2
  • Research in the Sullivan Lab is focused on chromosome organization, with a specific emphasis on the genomics and epigenetics of the chromosomal locus called the centromere. (duke.edu)
  • The centromere is a specialized chromosomal site involved in chromosome architecture and movement, and when defective, is linked to cancer, birth defects, and infertility. (duke.edu)
Sequences7
  • Researchers have completed a quest that started 32 years ago, having uncovered the final DNA sequences that make up a human genome. (genome.gov)
  • Which Sequences Are Present at the Centromeres? (cshlpress.com)
  • Alpha satellites are tandemly repeated sequences found in all human centromeres. (srce.hr)
  • All human alpha satellite sequences originate from one pra-ancestral alpha satellite monomer. (srce.hr)
  • A study published Thursday in the journal Science Advances sheds light on large chunks of genomic black holes by revealing the sequences of many human centromeres, the middle regions of our chromosomes essential for cell division. (fredhutch.org)
  • With that method, researchers were able to capture sequences at the edges of centromeres but not the centromeres themselves. (fredhutch.org)
  • Other research teams had modeled what human centromeres might look like based on their immediate flanking sequences. (fredhutch.org)
Antibody1
  • Methods Antibodies of antibody-secreting cells in human salivary glands were produced as recombinant antibodies. (elsevierpure.com)
Centromeric1
  • COURSE GOALS: The course goal is to introduce students to ways of computer identification of repeats and high-order repeats (HOR) and its structure for centromeric region of human genome in GenBank data and to give a preliminary insight into relevant actual theoretical literature. (pmf.hr)
Assembly3
  • Consequently, it has been difficult to achieve genome assemblies at centromeres using traditional next generation sequencing approaches, so that centromeres represent gaps in the current human genome assembly. (duke.edu)
  • Conversely, the simple presence of alpha satellite DNA is not sufficient for centromere function since chromosomes with multiple alpha satellite arrays only exhibit a single location of centromere assembly. (duke.edu)
  • Glunčić M, Rosandić M, Jelovina D, Dekanić K, Vlahović I, Paar V. Global Repeat Map Method for Higher Order Repeat Alpha Satellites in Human and Chimpanzee Genomes (Build 37.2 Assembly). (srce.hr)
Conditional1
  • Conditional labelling experiments show that they both assemble de novo during S phase and G2, increasing approximately three-to fourfold in abundance at centromeres. (nuigalway.ie)
Mammalian1
  • When comparing the human genome to related primate or mammalian genomes two major classes change are: single base pair substitutions and larger structural rearrangements such as insertion of new DNA. (escholarship.org)
Epithelial cells2
  • In primary cultures of human bronchial epithelial cells (HBECs) with 16.6±0.4 mM glucose in the basolateral medium, ASL glucose was 2.2±0.5 mM [ 11 ]. (ersjournals.com)
  • Finally, we demonstrated that CNF induced predominately centromere-positive MN in primary human small airway epithelial cells (SAEC) indicating aneugenic events. (cdc.gov)
Cells8
  • A stem-cell derived human embryo model showing blue cells (embryo), yellow cells (yolk sac) and pink cells (placenta). (planer.com)
  • As each chromosome contains a single centromere, it remains unclear how acentric fragments derived from shattered chromosomes are inherited between daughter cells during mitosis 6 . (nature.com)
  • The ability to knock out human genes using CRISPR technology has opened to door to testing for essential genes in tissue culture cells. (blogspot.com)
  • A classic paper that describes the limited replicative lifespan of normal human cells. (nature.com)
  • Researchers used stem cells obtained from the embryonic tissue of cynomolgus monkeys, a type of macaque commonly used in genetics research because of their similarity to humans. (planer.com)
  • In immortalised human airway cells (line H441) with 10 mM glucose in the basolateral medium, apical ASL glucose was 0.24±0.07 mM [ 10 ]. (ersjournals.com)
  • Centromeres are indispensable guideposts when cells split in two, providing an anchor point for cellular machinery to attach and pull as chromosomes duplicate and then segregate, one copy to each new cell. (fredhutch.org)
  • Those paired repeats dominated centromeres in the male and female human cells the researchers tested. (fredhutch.org)
Genomic2
  • Human centromeres typically form at large megabase-sized arrays of alpha satellite DNA for which there is little genomic understanding due to its repetitive nature. (duke.edu)
  • 2) also if #1 is ok, 98.6% genomic identity between chimp and human (= 22.4 million DNA bases) is highly debatable, because genomic comparison can be done in many different ways and depends on many presuppositions. (blogspot.com)
Repeats2
  • The outer regions, which aren't necessary for centromeres to work, have enough differences among the repeats that the jigsaw puzzle approach works - to a point. (fredhutch.org)
  • The closer you get to the actual centromere, the more identical the repeats become, and the technique starts to fall apart. (fredhutch.org)
Genes4
  • Presenting the genes of the human genome in their biological context, Guide to the Human Genome is an extensive online resource (also available in print) that provides easy access to information about human genes and their roles in specific processes. (cshlpress.com)
  • How many genes are in the human genome? (cshlpress.com)
  • How Does Codon Usage Vary Among Human Genes? (cshlpress.com)
  • The location of the centromere on each chromosome gives the chromosome its characteristic shape, and can be used to help describe the location of specific genes. (medlineplus.gov)
Mitotic2
  • the resulting mitotic configuration shows split centromeres and splayed chromatids in all or most of the chromosomes. (atlasgeneticsoncology.org)
  • The maintenance of a single centromere per chromosome is critical for establishing bipolar microtubule attachments to the mitotic spindle and achieving high-fidelity genome segregation 17 . (nature.com)
Aneuploidy2
  • A recent study has described why human embryos may exhibit such high levels of aneuploidy resulting in early pregnancy loss. (planer.com)
  • Humans exhibit very high rates of aneuploidy, despite being evolutionarily quite advanced. (planer.com)
Urine1
  • Arenaviruses are single-stranded ribonucleic acid (RNA) viruses that cause chronic asymptomatic infections in rodents and zoonotically acquired disease in humans through rodent excreta, especially urine. (medscape.com)
Identity1
  • Human Accelerated Region 1 (HAR1) is defined as the region of the human genome most significantly changed since we diverged from chimpanzees as it contains 18 substitutions in a 118bp region that has been conserved in our genomes from chicken to chimpanzee at 96% identity. (escholarship.org)
Mutants1
  • The analysis uses data from IMPC, along with published data on other mouse mutants, in comparison to human disease reports in OMIM, Orphanet, and DECIPHER. (mousephenotype.org)
Probe1
  • The Pinpoint FISH TP53/CEP 17 Probe Kit is intended to detect the copy number of the LSI TP53 probe target located at chromosome 17q11.1 and of the CEP 17 probe target located at the centromere of chromosome 17. (biocat.com)
Cenpb1
  • Synthetic peptide conjugated to KLH derived from within residues 550 to the C-terminus of Human CENPB. (abcam.com)
Variation1
  • Your degree and research area is bespoke to you, however, our supervisors can provide projects in many areas of current interest in genetics and genomics, ranging from fundamental questions in cell biology to variation and disease in humans. (nottingham.ac.uk)
Cellular1
  • I used molecular, genetic and biochemical methods to assign HAR1 RNAs a cellular function, and to test the hypothesis that the HAR1 containing RNAs contribute to human specific features of brain development. (escholarship.org)
Synonyme1
  • MyBiosourceProduct Short Name: [Creatine Kinase MB]Product Name Synonyme: [Creatine Kinase MB (CK-MB), Mouse Monoclonal anti-Human. (biocheminfo.org)
Mechanisms1
  • Our understanding of the role of glucose transport in the lung and the mechanisms that regulate glucose movement across the human lung epithelium lags far behind that of the gut and kidney. (ersjournals.com)
Mutations1
  • 3) also if #1,2 are ok, 130 mutations fixed in the human population in each generation is another highly debatable issue. (blogspot.com)
Molecular Genetics1
Species5
  • Telomeric Probes (sub-Telos) from KromaTiD are available for the p- and q-arms of all human chromosomes as standard products, other sequenced species are available by request. (biocat.com)
  • First Born - The discovery of a child's skeleton on the edge of the Kalahari desert gave rise to the theory of a species that straddled the boundary between human and ape. (topdocumentaryfilms.com)
  • Exodus - 'The discovery of bones, tools and artefacts at two archaeological sites on Africa's southern coast have helped scientists to calculate that people indistinguishable from the modern human species (homo sapiens) first appeared in Africa about 150,000 years ago. (topdocumentaryfilms.com)
  • This final programme in the series exploring evolution seeks similarities between the Neanderthals and modern humans and, drawing on the latest archaeological findings, re-creates the moment when the two species converged in Europe. (topdocumentaryfilms.com)
  • It was brought to the Americas from sub-Saharan Arfrica 500 ya and though hybridized, mostly with Europeans in N. America and the already- hybridized Bering-Strait migrated Central / S. Americans, it is now holding back the progression of the 2 superior modern human sub-species to the point that they well may be the cause of the failure of technolgy to save civilization as we know it. (topdocumentaryfilms.com)
Syndrome1
  • Other arenaviruses from South America and Africa are classic causes of viral hemorrhagic fever syndrome, whereas others have been identified but not found to cause disease or even infection in humans. (medscape.com)
Vitro4
  • Hayflick, L. The limited in vitro lifetime of human diploid cell strains. (nature.com)
  • In vitro studies in resistive human lung epithelial cell monolayers grown at an air-liquid interface have supported these in vivo observations. (ersjournals.com)
  • Human fibroblasts (0-20g/ml) were used to assess the predictive value of in vitro to in vivo modeling systems. (cdc.gov)
  • Human fibroblast monocultures were found to be insufficient to fully replicate in vivo fibrosis outcomes suggesting in vitro predictive potential depends upon more advanced cell culture in vitro models. (cdc.gov)
Similarity2
  • Phenotype comparisons summarize the similarity of mouse phenotypes with human disease phenotypes. (mousephenotype.org)
  • The table below shows human diseases predicted to be associated to Cenpc1 by phenotypic similarity . (mousephenotype.org)
NCBI1
  • The human genome following NCBI. (cshl.edu)
Mapping1
  • 15477. The public Human Genome Project: mapping the genome, sequencing, and reassembly. (cshl.edu)
Products2
  • Centromere Enumeration Probes (sub-CEPs) from KromaTiD are available for all human chromosomes as standard products. (biocat.com)
  • Whole Chromosome Pinpoint FISH Paints from KromaTiD are available for all human chromosomes as standard products. (biocat.com)
Fluorescent1
  • A portrait of human chromosomes: this process labels the chromosomes with multicolored fluorescent tags, allowing researchers to consistently distinguish between chromosomes. (cshl.edu)
Disease2
  • The target profiling of serum ACA from 269 patients with SS, systemic sclerosis (SSc), primary biliary cirrhosis (PBC) and healthy controls revealed that ACA-positive patients have antibodies against various sites of centromere complex regardless of disease. (elsevierpure.com)
  • Uniquely human features at the DNA level can be interrogated and likely hold the secrets to human evolution of specific traits as well as disease. (escholarship.org)