Loading...
  • cDNA
  • Southern blot analysis of the genomic DNA from 17 individuals showed perfect correspondence between a Bsp 12861 restriction fragment length polymorphism (RFLP) and the Ag(c/g) locus on human apolipoprotein (apo) B. The RFLP polymorphism is caused by a C--------T transition at nucleotide 421 on the cDNA, resulting in a threonine-to-isoleucine conversion. (ahajournals.org)
  • Initial assessment of human gene diversity and expression patterns based upon 83 million nucleotides of cDNA sequence" (PDF). (wikipedia.org)
  • Sequence
  • This approach should be applicable to pedigree and linkage analysis with the apolipoprotein B gene or other tandemly repeated sequence elements. (biomedsearch.com)
  • Sequence analysis suggested that putative CREBH response element (CREBHRE) is located in the human APOA5 promoter region and is highly conserved in both human and rodent. (hindawi.com)
  • However, two naturally occurring point mutations in human apoC3 coding sequence, namely Ala23Thr and Lys58Glu have been shown to abolish the intracellular assembly and secretion of triglyceride-rich VLDL particles from hepatic cells. (wikipedia.org)
  • apoE
  • Methods - Aged apoE knock out (KO) mice, mice overexpressing APP751 in the apoE KO background and mice expressing either human apoE3 or apoE4 and APP751 in the apoE KO background were exposed to permanent occlusion of the middle cerebral artery (MCA). (ahajournals.org)
  • Results - Overexpression of human APP751 significantly increased the infarct volumes in apoE KO mice. (ahajournals.org)
  • Furthermore, this APP751-induced ischemic vulnerability was attenuated by the coexpression of either human apoE isoform. (ahajournals.org)
  • To test the hypothesis that apoE3, but not apoE4, protects against age-related and excitotoxin-induced neurodegeneration, we analyzed apoE knockout ( Apoe −/− ) mice expressing similar levels of human apoE3 or apoE4 in the brain under control of the neuron-specific enolase promoter. (jneurosci.org)
  • These differences in the effects of apoE isoforms on neuronal integrity may relate to the increased risk of Alzheimer's disease and to the poor outcome after head trauma and stroke associated with apoE4 in humans. (jneurosci.org)
  • Apolipoprotein E (ApoE) is a class of proteins involved in the metabolism of fats in the body. (wikipedia.org)
  • Recent findings with apoA-I and apoE suggest that the tertiary structures of these two members of the human exchangeable apolipoprotein gene family are related. (wikipedia.org)
  • They are enzyme coenzymes (C-II for lipoprotein lipase and A-I for lecithin-cholesterol acyltransferase) Lipid transport proteins Ligands for interaction with lipoprotein receptors in tissues ( apoB100 and apoE for LDL-receptors, apoA-I for HDL receptors) There are two major types of apolipoproteins. (wikipedia.org)
  • novel
  • Here, we report CREBH as a novel regulator of human APOA5. (hindawi.com)
  • X. Prieur, H. Coste, and J. C. Rodríguez, "The human apolipoprotein AV gene is regulated by peroxisome proliferator-activated receptor- α and contains a novel farnesoid X-activated receptor response element," Journal of Biological Chemistry , vol. 278, no. 28, pp. 25468-25480, 2003. (hindawi.com)
  • APOA5
  • To clarify whether the human APOA5 promoter is regulated by CREBH, we analyzed the human APOA5 promoter region using a transient transfection assay and determined that transfection of CREBH induced human APOA5 promoter activity. (hindawi.com)
  • Here, we report that a CREBHRE is located in the promoter of human APOA5, which is a critical region for the transcriptional regulation of the APAO5 gene by CREBH. (hindawi.com)
  • Journal of Biolog
  • Apolipoprotein A5, a crucial determinant of plasma triglyceride levels, is highly responsive to peroxisome proliferator-activated receptor α activators," Journal of Biological Chemistry , vol. 278, no. 20, pp. 17982-17985, 2003. (hindawi.com)
  • plasma
  • Especially, it has been reported that CREBH is required for the maintenance of normal plasma TG levels, and hypomorphic or null mutations of CREBH associated with hypertriglyceridemia in human [ 14 ]. (hindawi.com)
  • In contrast, incubation of isolated human apoA-II with control mouse plasma at 37°C decreased PON1 activity and displaced the enzyme from HDL. (ahajournals.org)
  • Lp(a) carries cholesterol and binds atherogenic proinflammatory oxidized phospholipids as a preferential carrier of oxidized phospholipids in human plasma, which attract inflammatory cells to vessel walls and leads to smooth muscle cell proliferation. (wikipedia.org)
  • Human plasma apolipoproteins A-IV-0 and A-IV-3. (wikipedia.org)
  • The product of this gene is one of the minor apolipoproteins found in plasma. (wikipedia.org)
  • mammalian
  • Since apo(a)/Lp(a) derived rather recently in mammalian evolution - only old world monkeys and humans have been shown to harbour Lp(a) - its function might not be vital, but just evolutionarily advantageous under certain environmental conditions, e.g. in case of exposure to certain infectious diseases. (wikipedia.org)