Human etoposide drug screening assays antitumor. Medical search. Frequent questions

MTT assay was applied for evaluation of cytotoxicity assay. (ac.ir)
Based on this method, we performed cytotoxicity assays on 42 AsPCs grown both as monolayers and spheroids. (biomedcentral.com)
In line with these observations, the allogeneic CAR T cells showed superior cytotoxicity in in vitro assays, and they efficiently targeted primary multiple myeloma cells in an ex vivo model. (liangyihui.net)

Topoisomerase II activity in nuclear extracts Topoisomerase II activity in nuclear extracts, either vehicle controlled or incubated for different times with SP600125, was assayed using an assay kit based on the decatenation of kinetoplast DNA. (alk-inhibitors.com)
Human topoisomerase 1B regulates the topological state of supercoiled DNA enabling all fundamental cell processes. (mdpi.com)
The interaction of human topoisomerase 1B and dimethylmyricacene, a compound prepared semisynthetically from myricanol extracted from Myrica cerifera root bark, was investigated using enzymatic activity assays and molecular docking procedures. (mdpi.com)
Etoposide inhibits topoisomerase II , thus stopping DNA replication. (medchemexpress.com)

We then screened a patient-derived xenograft library to identify SCLC as a tumor type with enhanced sensitivity to calicheamicin ADCs. (aacrjournals.org)
miRNAs and lncRNAs control drug sensitivity and the development of tumor resistance towards platinum drugs. (eurekaselect.com)
This mini-review summarizes the resistance mechanisms of gliomas to platinum drugs, as well as molecules and therapies that can improve the sensitivity of gliomas to platinum drugs. (eurekaselect.com)
Early publications examined the relationship between specific genetic alterations in lung cancer and clinically relevant applications including differential drug sensitivity, differentiation of metastases from second primary cancers, and application of patient-specific mutations as epitopes for immunotherapy. (dukecancerinstitute.org)
Correlation of alteration of p16 with drug sensitivity led to identification of a class of CDK4 inhibitor. (dukecancerinstitute.org)
We have developed a 3D (spheroid) functional assay to assess the sensitivity of two PARPis (niraparib and olaparib) in ascites-derived primary cell cultures (AsPCs) from HGSOC patients. (biomedcentral.com)
Global gene expression comparison of 6 PARPi-resistant and 6 PARPi-sensitive 3D AsPCs was indicative for the predominant downregulation of numerous genes and networks with previously demonstrated roles in EOC chemoresistance, suggesting that the PARPis-sensitive AsPCs could display enhanced sensitivity to other chemotherapeutic drugs, commonly applied in cancer management. (biomedcentral.com)
All Luminex assays are tested for sensitivity, intra-assay precision, inter-assay precision and to ensure assay linearity for validated sample types. (bio-techne.com)
Therefore, elimination of the checkpoint increases the sensitivity of human tumor cell lines to anticancer agents. (hsp90-inhibitors.com)

Sphingolipid-targeting drugs have been tested alone or in combination with chemotherapy, exhibiting antitumor activity alone and in synergism with chemotherapy in vitro and in vivo . (frontiersin.org)
In vitro tubulin polymerization assay We prepared 50 ?l of 5 mg ml tubulin at a steady state by incubation at 37 for 30 min in G PEM buffer containing 10 glycerol in a 96 well plate. (alk-inhibitors.com)
To benefit from those effects, it is required that the multitarget drug exhibit balanced in vitro and in vivo activities to match potency for the corresponding targets, as well as optimized PK and safety profiles. (biomedcentral.com)
their structures were elucidated using spectroscopic methods and their bioactivity was evaluated using an in vitro microtubule assembly assay. (liverpool.ac.uk)
taxotere inhibits in-vitro growth of human colonic cancer cell lines. (liverpool.ac.uk)

Ideally, a drug that inhibits PKB should cause both cell cycle arrest and promote apoptosis. (justia.com)

MTT colorimetric assays, colony formation assays and 5‑ethynyl‑20‑deoxyuridine incorporation assays were also conducted to evaluate ACC cell proliferation. (cancerindex.org)
CCK-8 and colony formation verified the various effects of Etoposide on different subtypes of LUAD cell lines. (biomedcentral.com)

The effectiveness or lack of a major therapeutic effect of sphingolipid modulation by some drugs as a cancer therapy and other aspects related to their mechanism of action are discussed in this review. (frontiersin.org)
Moreover, after antitumor treatment, [ 89 Zr]Zr-DFO-KN035 enabled observational imaging for therapeutic efficacy evaluation, which can help predict patient prognosis. (thno.org)
ARV-SNEP could be potentially novel therapeutic approach for the treatment of drug-resistant melanoma. (nadph-oxidasesignaling.com)
Approved and experimental epigenetic drugs are undergoing clinical trials in combination with other therapeutic agents via fused or linked pharmacophores in order to benefit from synergistic effects of polypharmacology. (biomedcentral.com)
Another current trend in therapy is drug repurposing or the re-discovery of a new therapeutic area for a drug used traditionally to treat a given pathology, either through the ability to modulate an additional target or by the involvement of the primary target in multiple pathologies. (biomedcentral.com)
Screening of herbal plants for various therapeutic properties is the hour as it shows promising activity. (biomedcentral.com)
The combination therapy of Etoposide with NK-252 administered p.o. significantly increases the life-span of mice inoculated i.p. with P388/S compared with the corresponding therapeutic effects with Etoposide alone. (medchemexpress.com)

Platinum drugs are a class of chemotherapeutic drugs that affect the cell cycle, and the main site of action is the DNA of cells, which are common chemotherapeutic drugs in clinical practice. (eurekaselect.com)
There are a number of strategies in the administration of chemotherapeutic drugs used today. (worldsbest.rehab)

In cultured primary fibroblasts and cancer cells, the chemotherapeutic drug doxorubicin causes mtDNA damage and release, which leads to cGAS STING dependent ISG activation. (regenerativemedicine.net)

Etoposide is still considered a potent, well-tolerated combination partner in innumerable hematologic and solid tumor treatment schedules, including those for malignant lymphomas and lung cancer. (wikipedia.org)
Thus, the plant may act as a potent antimicrobial drug against resistant strains. (biomedcentral.com)

89 Zr]Zr-DFO-KN035 monitored and predicted the site of adverse reactions in antitumor immunotherapy. (thno.org)
As a novel antitumor therapy, tumor immunotherapy is attracting considerable attention and extensive research worldwide. (thno.org)
Under the tumor immunotherapy strategy, various monoclonal antibody drugs are being developed and marketed to eliminate tumor cells by suppressing tumor immune-checkpoint signaling pathways and reactivating antitumor immune responses. (thno.org)

Microtubule binding drugs are of special interest as they have important roles in the modulation of cellular functions and many of them act as anticancer agents. (rgcb.res.in)

Among the advantages of multitarget drugs vs drug combinations are the more predictable pharmacokinetic (PK) and pharmacodynamic (PD) relationship of the components of a single medicine, the possibility that one motif might improve the bioavailability of the second entity, the greater efficacy against advanced-stage diseases, the lower toxicities, the simultaneous presence of the chemical entities in multiple tissues, and the improved patient compliance [ 2 ]. (biomedcentral.com)
A cholesterol-tethered platinum ii-based supramolecular nanoparticle increases antitumor efficacy and reduces nephrotoxicity. (cetbwh.org)

Development of a facile antibody-drug conjugate platform for increased stability and homogeneity. (cetbwh.org)

Luminex Assays are validated for use with cell culture supernatants, plasma and serum. (bio-techne.com)
The US Preventive Services Task Force (USPSTF) recommends against screening (with serum CA125 level or transvaginal ultrasonography) for ovarian cancer in the general population. (medscape.com)

Here, we describe a complementing xenograft protocol for testing CAR T cells against human leukemia cells in zebrafish embryos. (bvsalud.org)

The result indicates that, the combination of both calprotectin and etoposide is considerably less cytotoxic on gastric cancer cells (AGS) than applying individually. (ac.ir)

Efforts are underway to use chemoinformatics to help understand drug effects from a signal transduction network perspective [ 3 ], to confidently predict new molecular targets for known drugs, and to explain polypharmacology. (biomedcentral.com)
Likewise, though characterizing ashwa gandha and its molecular mechanisms Wadhwa et al provided the first instance that phytochemical have each anti cancer and anti senescent routines and pointed in the direction of the molecular website link in between aging and cancer using usual human fibroblasts by means of decreased accu mulation of molecular harm. (hsp70inhibitor.com)
6. The drug delivery device of claim 1, wherein the polyethyleneimine has a molecular weight between 20,000 and 50,000 Daltons. (patentsencyclopedia.com)

Decreased intracellular drug concentration, enhanced function of cell processing active products, enhanced repair ability of cellular DNA damage, and blockage of related apoptosis pathways play an important role in it. (eurekaselect.com)
Etoposide induces cell cycle arrest, apoptosis and autophagy . (medchemexpress.com)

Teniposide and etoposide were found by Stähelin to have a new mechanism of action: they blocked the entry of cells into mitosis (with arrest in late S or G2 phase), in contrast to SP-G and SP-I, which were spindle poisons. (wikipedia.org)
It is known that the pathogenic properties of glioma cells and the response of glioma towards platinum-based drugs are strongly influenced by non-coding RNAs, particularly, by microRNAs (miRNAs) and long non-coding RNAs (lncRNAs). (eurekaselect.com)
Many of the side effects of chemotherapy can be traced to damage to normal cells that divide rapidly and are thus sensitive to anti-mitotic drugs: cells in the bone marrow, digestive tract and hair follicles. (worldsbest.rehab)
Because of the effect on immune cells (especially lymphocytes), chemotherapy drugs often find use in a host of diseases that result from harmful overactivity of the immune system against self (so-called autoimmunity). (worldsbest.rehab)
Drugs used in chemotherapy, such as temozolomide, work in different ways to stop the growth of tumor cells, either by killing the cells or by stopping them from dividing. (ucbraid.org)
In one from the studies, using non modest lung adenocar cinoma A549 cells, it was proven that right after therapy with DNA damaging anti tumor drugs like caffeine, cells grow to be permanently growth arrested as being a end result of so known as drug induced more hints premature senescence or SIPS. (hsp70inhibitor.com)

tyrosine kinase inhibitors are the most important drugs in the cml therapy and provide long disease-free survival. (liverpool.ac.uk)

Lymphocyte activation gene 3 (LAG3) is an inhibitory immune checkpoint receptor that restrains autoimmune and antitumor responses, but its evolutionarily conserved cytoplasmic tail lacks classical inhibitory motifs. (bvsalud.org)

Therefore, the relationship between autophagy and cell death remains unclear and warrants further study to harness autophagy for the treatment of various human diseases. (nature.com)

Further chemical modifications led to the well-known thenylidene derivative VM-26 (teniposide, Vumon, discovered in October 1965, commercialized by Sandoz in 1976) and to the ethylidene derivative VP-16-213 (etoposide, Vepesid, discovered on 21 October 1966), which remains very clinically successful. (wikipedia.org)
Direct correlation between resistance to chemotherapy and activation of PKB was shown in several prostate cancer cell lines and in human tumorogenic tissues, and elevation of PKB levels in prostate tumor tissues, is clinically associated with HRPC (Yongde et al. (justia.com)

Etoposide is a lipophilic agent that can easily transport from membrane while calprotectin active intracellular pathway, probably by membrane surface receptor. (ac.ir)

Although polypharmacology might be associated with compound promiscuity, it should be considered a different category since the so-called promiscuous drugs are instead a class of compound that show a wide spectrum of biological activities and adverse reactions [ 1 ]. (biomedcentral.com)

The protein concentration was quantified using a Bio Rad detergentcompatible protein assay reagent. (alk-inhibitors.com)
PKB is an anti-apoptotic protein kinase whose activity is strongly elevated in human malignancies. (justia.com)
ADI-TRAIL fusion protein was efficacious in a number of cell lines and synergized with some standard of care drugs. (oncotarget.com)

The modern drug discovery process has largely focused its attention in the so-called magic bullets, single chemical entities that exhibit high selectivity and potency for a particular target. (biomedcentral.com)
Epigenetic drug discovery is a rapidly advancing field, and drugs targeting epigenetic enzymes are in the clinic for the treatment of hematological cancers. (biomedcentral.com)
Luminex Discovery Assays provide the most flexible assays on the market. (bio-techne.com)
These panels are completely customizable using our large menu of human, mouse, and rat analytes, making them the perfect tool for biomarker discovery and screening. (bio-techne.com)
The mouse discovery Luminex Assay is also validated for use with tissue lysates. (bio-techne.com)
Assays for the Luminex platform are offered as High Performance Assays or Discovery Assays . (bio-techne.com)
The Luminex Discovery Assays allow for the maximum number of analytes in a multiplex and is supplied as a premixed kit. (bio-techne.com)

Additionally, based on the properties summarised previously, it is proposed that itraconazole may synergise with a range of other drugs to enhance the anti-cancer effect, and some of these possible combinations are presented in the supplementary materials accompanying this paper. (ecancer.org)
Chemotherapy may be given with a curative intent (which almost always involves combinations of drugs) or it may aim to prolong life or to reduce symptoms (palliative chemotherapy). (worldsbest.rehab)
The human gastric adenocarcinoma cell line was exposed to different concentrations and combinations of calprotectin and etoposide. (ac.ir)

Antibacterial activity was evaluated by using disc and agar diffusion methods and the anti-cancer effect of the rose flower extract (RE) was examined using MTT assay in lung cancer cell line. (biomedcentral.com)

9. A method for treating or preventing adverse effects of an antibiotic to the intestinal flora, comprising administering the drug delivery device of claim 1 to a patient, either before, during, or after administration of the antibiotic. (patentsencyclopedia.com)

After a landmark clinical comparative analysis by F. Muggia and M. Rozencweig, etoposide and teniposide were licensed to Bristol-Myers in 1978 for further clinical development. (wikipedia.org)
The objective of this review was to analyze the results from preclinical and clinical trials of these drugs for the treatment of cancer. (frontiersin.org)
Aslthough all these drugs have produced good results in preclinical studies of multiple cancers, the outcomes of clinical trials have not been similar. (frontiersin.org)
In addition, the regulatory requirements are more complex when the agents are used in combination, since the safety profile of each drug needs to be demonstrated before clinical trials, and this can be further delayed due to regulatory and IP issues, in particular if the two drugs are being developed by different companies [ 1 ]. (biomedcentral.com)
Using two confirmed chordoma cell lines, I screened all regulatory-approved drugs for anti-growth activity to determine whether any could be repurposed for clinical use in patients. (dukecancerinstitute.org)
Itraconazole, a common triazole anti-fungal drug in widespread clinical use, has evidence of clinical activity that is of interest in oncology. (ecancer.org)
Predicting clinical response to anticancer drugs using an ex vivo platform that captures tumour heterogeneity. (cetbwh.org)
The application of this assay and the potential biomarkers with possible predictive significance to PARPis therapy of EOC patients now need testing in the setting of a clinical trial. (biomedcentral.com)

To interrogate for epigenetic dependencies, we performed a CRISPR screen and show that patient-derived H3K27M-glioma neurospheres are dependent on core components of the mammalian BAF (SWI/SNF) chromatin remodeling complex. (dana-farber.org)

Current chemotherapy regimens apply drug treatment in cycles, with the frequency and duration of treatments limited by toxicity. (worldsbest.rehab)

Therefore, the abnormal regulation of PCD is associated with numerous human diseases, including cancers and neurodegenerative diseases. (nature.com)
Disruption of normal PKB/Akt signaling has now been documented as a frequent occurrence in several human cancers and the enzyme appears to play an important role in their progression (Nicholson and Anderson, Cellular Signalling 14, 381, 2002). (justia.com)

Their current application in oncology has revolutionized cancer treatment by improving the antitumor activity of several chemotherapeutic agents. (mdpi.com)

ITZ is commonly available as a generic, prescription-only drug. (ecancer.org)
Application of combined toxic agents such as etoposide and cicplatin are commonly used for chemotherapy purposes. (ac.ir)

the ability of the artus epstein-barr virus (ebv) pcr kit and the abbott realtime ebv pcr assay to detect and quantify plasma ebv dnaemia was compared. (liverpool.ac.uk)

Pizzocaro G, Piva L, Salvioni R, Zanoni F, Milani A. Cisplatin, etoposide, bleomycin first-line therapy and early resection of residual tumor in far-advanced germinal testis cancer. (eurekaselect.com)
This is the very ﬁrst paper investigating a PROTAC class of molecule for the treatment of drug resistant cancer, preformulation and formulation studies. (nadph-oxidasesignaling.com)
However, the use of cocktails or multicomponent drugs to address several targets simultaneously is also popular to treat multifactorial diseases such as cancer and neurological disorders. (biomedcentral.com)
Chemotherapy (often abbreviated to chemo and sometimes CTX or CTx ) is a type of cancer treatment that uses one or more anti-cancer drugs (chemotherapeutic agents or alkylating agents) as part of a standardized chemotherapy regimen. (worldsbest.rehab)
Importantly, the use of drugs (whether chemotherapy, hormonal therapy or targeted therapy) constitutes systemic therapy for cancer in that they are introduced into the blood stream and are therefore in principle able to address cancer at any anatomic location in the body. (worldsbest.rehab)
epstein-barr virus (ebv) is a human γ-herpesvirus associated with cancer, including burkitt lymphoma, nasopharyngeal, and gastric carcinoma. (liverpool.ac.uk)

Notwithstanding the success of combination therapy, the use of a single drug that modulates several targets might be therapeutically advantageous over the use of drugs in combination. (biomedcentral.com)
Examples include the use of the anti-angina drug sildenafil to treat sexual disfunction or the infamous sedative thalidomide as therapy for multiple myeloma. (biomedcentral.com)

In the past year, four antibody-drug conjugates (ADC) were approved, nearly doubling the marketed ADCs in oncology. (aacrjournals.org)

1. Oral drug delivery devices for colonic release of active ingredients, comprising:a) an active agent capable of inactivating an antibiotic, andb) a drug delivery device comprising pectin beads, where the pectin is crosslinked with calcium ions, and reticulated with polyethyleneimine. (patentsencyclopedia.com)

Chemotherapy with platinum drugs such as cisplatin, carboplatin, oxaliplatin, or a combination thereof is used to treat a variety of tumors. (eurekaselect.com)

A comprehensive kinase interaction map for dasatinib was recently described, though assay differences preclude direct comparison of the kinase users of AP24534 and dasatinib. (others-signal.com)

In addition, ligands are being discovered which, as single chemical entities, are able to modulate multiple epigenetic targets simultaneously (multitarget epigenetic drugs). (biomedcentral.com)
These multiple ligands should in principle have a lower risk of drug-drug interactions and drug resistance compared to cocktails or multicomponent drugs. (biomedcentral.com)
Computationally designed antibody-drug conjugates self-assembled via affinity ligands. (cetbwh.org)

A few years later, in November 1983, etoposide was approved by the U.S. Food and Drug Administration. (wikipedia.org)

lymphoblastoid cell lines (lcls) transformed by epstein-barr virus (ebv) serve as an unlimited resource of human genomic dna. (liverpool.ac.uk)

As a matter of fact, calprotectin and etoposide were both applied on human gastric adenocarcinoma cell line (AGS) as antitumor agents. (ac.ir)

Therefore, it is crucial to screen both the whole plant and individual components to determine its medicinal value. (biomedcentral.com)

In this issue of Blood, Shin et al expand our understanding of clonal hematopoiesis (CH) by showing its natural emergence in aged non-human primates (rhesus macaques), and by demonstrating robust expansion of TET2-mutated clones causing hyperinflammation in a CRISPR-Cas9-based autologous transplantation model in macaques. (dana-farber.org)
The overuse of antibiotics in the past decades has led to the emergence of a large number of drug-resistant microorganisms. (mdpi.com)

However, the results of gliomas chemotherapy are unsatisfactory, and resistance to platinum drugs is one of the important reasons. (eurekaselect.com)
The resistance of gliomas to platinum drugs is the result of a combination of influencing factors. (eurekaselect.com)

In 1949, nitrogen mustard (mechlorethamine) became the first chemotherapeutic agent approved by the U.S. Food & Drug Administration, which was rapidly followed by a wave of chemotherapy development 1 . (cancerbiomed.org)

Despite the decline in CRC incidence and mortality over the last decade, mainly attributed to the improvement in early screening methods, CRC still remains incurable and highly lethal in the advanced stage ( 3 ). (frontiersin.org)
Calprotectin was purified from human neutrophil by chromatography methods. (ac.ir)

Arctigenin ((-)-Arctigenin), a biologically active lignan, can be used as an antitumor agent. (medchemexpress.com)
Drug delivery devices that are orally administered, and that release active ingredients in the colon, are disclosed. (patentsencyclopedia.com)
2. The drug delivery device of claim 1, wherein the amount of polyethyleneimine is sufficient to allow a substantial portion of the pectin beads to pass through the gastrointestinal tract to the colon without releasing the active agent, and is also sufficient such that the pectin beads are sufficiently degraded in the colon to release an effective amount of the active agent. (patentsencyclopedia.com)
3. The drug delivery device of claim 1, wherein the active agent is an enzyme capable of inactivating beta-lactam antibiotics. (patentsencyclopedia.com)

In 1969, Stähelin made the key decision to include a test system for immunosuppression into the general screening program at Sandoz for compounds and extracts. (wikipedia.org)

An animal model of the most common MYH9 mutation was created in my lab and demonstrated hematological abnormalities similar to those found in humans. (dukecancerinstitute.org)

A standard curve must be generated each time an assay is run, utilizing values from the Certificate of Analysis provided with each Luminex Assay. (bio-techne.com)

The drug delivery devices are in the form of beads of pectin, crosslinked with calcium and reticulated with polyethyleneimine. (patentsencyclopedia.com)

He discovered two important drugs: etoposide and ciclosporin. (wikipedia.org)

Polypharmacology refers to the ability of drugs to interact simultaneously and specifically with multiple targets (multitarget drugs). (biomedcentral.com)

Anatomy10

Organisms7

Diseases41

Chemicals and Drugs61

Analytical, Diagnostic and Therapeutic Techniques and Equipment36

Phenomena and Processes25

Information Science1

Health Care4

Cytotoxicity3

Topoisomerase4

Sensitivity9

Vitro5

Inhibits1

Colony formation2

Therapeutic7

Chemotherapeutic drugs2

Doxorubicin1

Potent2

Immunotherapy3

Microtubule1

Efficacy2

Antibody-Drug Conjugate1

Serum2

Xenograft1

Cytotoxic1

Molecular3

Apoptosis2

Cells6

Survival1

Immune1

Autophagy1

Clinically2

Receptor1

Compound1

Protein3

Discovery7

Combinations3

Lung cancer1

Adverse1

Clinical8

Patient-derived1

Chemotherapy regimens1

Cancers2

Activity1

Commonly2

Detect1

Cancer6

Therapy2

Oncology1

Colonic1

Carboplatin1

Interaction1

Ligands3

Food and Drug Admin1

Cell lines1

Agents1

Components1

Emergence2

Resistance2

Development1

Methods2

Active4

Compounds1

Hematological1

Analysis1

Beads1

Important drugs1

Targets1